Hippo Signaling
Transcript of Hippo Signaling
For pathway key and background, please visit:
www.cst-science.com/hippo
Hippo Signaling
SCFβTrCP
AMOT
Hippo Signaling
RT
K
RT
K
Crb YAP/TAZYAP/TAZ YAP/TAZ
YAP/TAZ
AMOTCrb
AMOT
AMOT
PTPN14
PTPN14
FRMD6
FRMD6
DestructionComplex
aPKC
α-Cateninα-Catenin
14-3-314-3-3
14-3-3
Ajuba/LIM
YAP/TAZ
ON-State (YAP/TAZ Inactive)OFF-State (YAP/TAZ Active)
Cytoplasm
Nucleus Cytoplasm
Nucleus
Target Gene Transcription
YAP/TAZ YAP/TAZ YAP/TAZ
YAP/TAZ
YAP/TAZYAP/TAZ
YAP/TAZ
LATS1/2
MST1/2
YAP/TAZ
YAP/TAZ YAP/TAZ
YAP/TAZYAP/TAZ
TEADs ERBB4 EGR-1
MOB1SAV1
MAPK
AMPK TAO1-3
MAPK4Ks
Rho
Rho
TBX5 SMADs RUNXTEADs ERBB4
PRP4K
EGR-1 TBX5 SMADs RUNX
GPCRSignaling
Growth FactorSignaling
ECM GPCRSignaling
Integrins
ECM/MechanicalSignaling
Integrins
ZO-2
Adhe
rens
Jun
ctio
nTi
ght J
unct
ion
ZO-2
Hippo Kinase Core
LATS1/2
MST1/2
MOB1
SAV1
Hippo Kinase Core
Ajuba/LIM
Adhe
rens
Jun
ctio
nTi
ght J
unct
ion
NF2NF2
YES1
ABL
NF2NF2
Ras PI3K
PDK1 MARK4 RASSF
STRIPAK
SLMAP
STRIPAK
SLMAP
KIBRA KIBRA
SRC
SAV1NF2
PAR
CK1
Gα12/13
Gαq/11
Gαi/o
ActinCytoskeleton
GαS
YAP/TAZSequestration
YAP/TAZSequestration
YAP/TAZDegradation
ActinCytoskeleton
rev. 02/14/20
No ContactInhibition
ContactInhibition
For R
esea
rch
Use
Only
. Not
For
Use
In D
iagn
ostic
Pro
cedu
res.
19-
CEP-
2995
3-PW
H
© 2020 Cell Signaling Technology, Inc. All trademarks are the property of their respective owners. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom. For the most up-to-date trademark information, please visit www.cellsignal.com/trademarks
For pathway key and background, please visit:
www.cst-science.com/hippo
Key Products for Hippo SignalingVisit www.cellsignal.com for a full listing of antibodies, related kits, and reagents.
ANTIBODIES APPLICATIONS REACTIVITY
34648 Ajuba (D4D8P) Rabbit mAb WB, IP H, Mk
43130 Angiomotin (D2O4H) Rabbit mAb WB, IP, IF-IC H
14688 FRMD6 (D8X3R) Rabbit mAb WB, IP H, M, R
8774 KIBRA Antibody WB, IP H, Mk
3477 LATS1 (C66B5) Rabbit mAb WB, IP H, M, Mk
8654 Phospho-LATS1 (Thr1079) (D57D3) Rabbit mAb
WB H, M
9153 LATS1 Antibody WB H, Mk
9157 Phospho-LATS1 (Ser909) Antibody WB H
5888 LATS2 (D83D6) Rabbit mAb WB H
13245 LIMD1 Antibody WB H, M
92427 MAP4K3 (D1L4G) Rabbit mAb WB, IP H, Mk
6995 Merlin (D1D8) Rabbit mAb WB, IP H, M, R
12888 Merlin (D3S3W) Rabbit mAb WB, IP, IF-IC H, M, R, Hm, Mk
12896 Merlin (D6N8H) Rabbit mAb WB, IP H, M, R, Hm, Mk
13730 MOB1 (E1N9D) Rabbit mAb WB, IP H, M, R, Hm, Mk
8843 Phospho-MOB1 (Thr12) (D2E3) Rabbit mAb
WB H, M, R, Mk
8699 Phospho-MOB1 (Thr35) (D2F10) Rabbit mAb
WB, IHC-P H, M, R, Mk
3863 MOB1 Antibody WB H, M, R
14946 MST1 (D8B9Q) Rabbit mAb WB, IP H, M, R
49332 Phospho-MST1 (Thr183)/MST2 (Thr180) (E7U1D) Rabbit mAb
WB H, M
3952 MST2 Antibody WB, IP H, M, R, Mk, B
8577 PRP4K (D27A1) Rabbit mAb WB, IP H, R, Mk
13808 PTPN14 (D5T6Y) Rabbit mAb WB H, M, R
86026 RASSF1 (E7T6O) Rabbit mAb WB H
2117 RhoA (67B9) Rabbit mAb WB H, M, R, Mk, B
13301 SAV1 (D6M6X) Rabbit mAb WB, IP, IF-IC H, M
3507 Sav1 Antibody WB, IP H, R, Mk
70148 TAZ (D3I6D) Rabbit mAb WB, IP, ChIP, ChIP-seq H, M
83669 TAZ (E8E9G) Rabbit mAb WB, IP, IF-IC, ChIP H, M, R, Mk
59971 Phospho-TAZ (Ser89) (E1X9C) Rabbit mAb WB, IP, IHC-P H, M, R
71192 TAZ (E5P2N) Mouse mAb WB, IP H, M, R, Mk
ANTIBODIES APPLICATIONS REACTIVITY
4883 TAZ (V386) Antibody WB, ChIP H, M, R
72804 TAZ (E9J5A) XP® Rabbit mAb WB, IHC-Bond, IHC-P H, M, Mk
13295 Pan-TEAD (D3F7L) Rabbit mAb WB, IP H, M, R, Mk
12292 TEAD1 (D9X2L) Rabbit mAb WB, IP, IHC-P, IF-IC H, M, Mk
13224 TEAD3 Antibody WB, IP H, M, R
11984 ß-TrCP (D12C8) Rabbit mAb WB H, M, R, Mk
11831 WBP2 Antibody WB H, M, R, Mk
14074 YAP (D8H1X) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq
H, M, R, Hm, Mk
53749 Phospho-YAP (Ser109) (E5I9G) Rabbit mAb
WB, IP H, M, R, Mk
13008 Phospho-YAP (Ser127) (D9W2I) Rabbit mAb
WB, IP, IHC-P H, M, R
13619 Phospho-YAP (Ser397) (D1E7Y) Rabbit mAb
WB, IP H, M, R
12395 YAP (1A12) Mouse mAb WB, IP, IHC-P H, M, R, Hm, Mk
4912 YAP Antibody WB, IP H, M, R, Mk
4911 Phospho-YAP (Ser127) Antibody WB H, M, R
46931 Phospho-YAP (Ser109) Antibody WB, IP H, M, R, Mk
29495 Non-phospho (Active) YAP (Ser127) (E6U8Z) Rabbit mAb
WB H
8418 YAP/TAZ (D24E4) Rabbit mAb WB, IP, IHC-P H, M, Mk
KITS
56612 Hippo Pathway: Upstream Signaling Antibody Sampler Kit
8579 Hippo Signaling Antibody Sampler Kit
48804 PathScan® Phospho-YAP (Ser127) Sandwich ELISA Kit
ELISA H
57046 PathScan® Phospho-YAP (Ser397) Sandwich ELISA Kit
ELISA H
32939 PathScan® Total YAP Sandwich ELISA Kit
ELISA H, M
52420 Phospho-YAP/TAZ Antibody Sampler Kit
56674 YAP/TAZ Transcriptional Targets Antibody Sampler Kit
TAZ (E9J5A) XP® Rabbit mAb #72804: IHC analysis of paraffin-embedded human breast carcinoma using #72804.
kDa
YAP
β-Actin
140
10080
60504030
20
10
5040
HeLa
– + YAP knock-out
200
YAP (D8H1X) XP® Rabbit mAb #14074: WB analysis of HeLa cell extracts, untreated (-) or YAP knock-out (+), using #14074.
C
YAP (D8H1X) XP® Rabbit mAb #14074: Confocal IF analysis of low confluence MCF 10A (A), high confluence MCF 10A (B), and YAP negative RL-7 (C) cells using #14074 (green). Blue pseudocolor in lower images = DRAQ5® #4084 (fluorescent DNA dye). Increased nuclear localization of YAP protein is seen in low confluence (proliferating) cells.
BA
TAZ (E9J5A) XP® Rabbit mAb #72804: IHC analysis of paraffin-embedded human colon carcinoma using #72804.