Hints Sheet for PEPCK Kinetics Paper
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Transcript of Hints Sheet for PEPCK Kinetics Paper
HintsSheetfor“InhibitionandAllostericRegulationofMonomericPhosphoenolpyruvateCarboxykinaseby3-MercaptopicolinicAcid”MarkD.Balan,MatthewJ.Mcleod,WilliamR.Lotosky,MarkGhaly,andToddHolyoakMetabolicpathwaythatPEPCKisinvolvedin:
PEPCK
O
O
-O
O
O-
oxaloacetate
P
O
O--O O
O
O-
phosphoenolpyruvate
Glycolysisroleinmetabolism:BreakdownglucosetocreateATPandpyruvate.PyruvateisthenusedinthecitricacidcycletoproducemoreATP.Gluconeogenesisroleinmetabolism:Makeglucosefromprecursorsincell,esp.pyruvate.
Hypoglycemicagent=somethingthatleadstolowlevelsofglucose(lowbloodsugar)P-loopmotif=Manyphosphate-bindingproteinshaveaphosphate-bindingloop(P-loop).TheconsensussequenceforthisstructureisGXXXXGKT/S.SkiptheExperimentalsectionunlessyouareinterestedinhowtodocrystallography(wewillnotdiscussitinclass).ReactionmechanismofPEPCK:from:"StructuralInsightsintotheMechanismofPhosphoenolpyruvateCarboxykinaseCatalysis"CarlsonandHolyoak,JBiolChem.2009Oct2;284(40):27037–27041.InthesectionoftheResultsandDiscussionenitledCrystallography,youdon'tneedtopayattentiontothedetailsofthestructuredetermination.Whentheytalkaboutdisorderedloopstheymeanthatsomeloopsintheproteinareflexibleandsoaremissingfromthex-raystructurebecauseitisa"snapshot"oftheproteinstructure.Anythinginmotionisnotclearlyvisible.