High-throughput Cytological Discovery System · cells labeled with multi-fluorescent dyes. Two...
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Yokogawa Technical Report English Edition Vol.60 No.2 (2017) New Products 67 119 In the field of new drug discovery, the high content analysis (HCA) system is becoming popular for screening several hundred thousand compounds to identify promising candidates for new drugs. In HCA, cells are imaged with a microscopic camera, and their temporal changes are analyzed. To observe biological changes in living cells and identify the effect of the candidate compounds, it is necessary to observe living cells for a long time. Also, there is an increasing need to eliminate the effect of fluorescent labeling in cells and observe cell behavior without labeling, although intracellular functional molecules and organelles are generally labeled with fluorescent dyes for HCA analysis. However, automatic image analysis of label-free cells is challenging because their image contrast is lower than that of labeled cells. The CellVoyager CV8000 satisfies these latest needs. MAJOR FEATURES Optimal cell-culture environment enabling long-term observation of living cells The incubator mechanism of the system has been redesigned to improve the sealing of the incubator. As a result, cells can be cultured in a more uniform environment. Software for analyzing images of label-free cells and 3D-cultured cells The CellVoyager CV8000 comes with CellPathfinder, which is new image analysis software that can recognize and analyze images of unlabeled cells with the help of pattern recognition and machine learning functions. This software also has a 3D image analysis function for observing 3D-cultured cells similar to those in living organisms. Quick imaging of multicolor-labeled cells The CV8000 can accommodate four cameras (three in the previous CellVoyager CV7000). The time required for imaging cells stained with four colors is reduced by more than 20% compared with the CV7000. The number of selectable laser wavelengths has been increased from four to five to offer wider options for experiments and various observations of cells labeled with multi-fluorescent dyes. Two Yokogawa Nipkow disks with industry-leading performance The CellVoyager series uses Yokogawa’s original confocal scanning disk technology based on the Nipkow disk. The conventional Yokogawa Nipkow disk with 50 µm pinhole diameter is suited for observing cells with weak fluorescence intensity. The CV8000 can also accommodate a disk with 25 µm pinhole diameter for capturing high-definition images especially with low- magnification objective lenses. Users can obtain clear images by using the two disks depending on the fluorescence intensity and the sample conditions. MAJOR SPECIFICATIONS ● Optical system Confocal unit: Microlens-enhanced wide-view Nipkow disk confocal scanner Excitation laser wavelength: 405 nm, 445 nm, 488 nm, 561 nm, 640 nm Transmission-illumination source: LED Objective lens: 2× to 60× (phase contrast, water immersion, long working distance) Camera: High-sensitivity sCMOS camera (up to 4 units) ● Environment control (stage incubator) Temperature control, CO2 conc. control, forced humidification ● Built-in dispenser (option) Designated disposable chips needed ● Output data format Image: TIFF, PNG Numerical data: CSV APPLICATION EXAMPLE ● Measurement of calcium flux into cells Ionomycin of various concentrations was added to cells using the dispenser of the CV8000. Kinetic changes in calcium concentration in the cells are shown in the graph below. Contact us: Life Science Center, Measurement Business Headquarters TEL: +81-76-258-7032 FAX: +81-76-258-7029 For worldwide locations, please see the back cover. * CellVoyager is a registered trademark of Yokogawa Electric Corporation. CO2 incubator CV8000 Top: Before incubation Bottom: After 68 hours of incubation CV8000 CO2 incubator Total area covered by cells at 68 hours of incubation (normalized by the area before incubation, average of 96 wells) 0 2 4 6 8 Ionomycin was applied to A10 cells pre-incubated with Fluo-4, which were imaged at 100 ms intervals. Mean intensity Mean intensity/ionomycin conc. Ionomycin conc. (nM) 0.00 19.53 78.13 312.50 1250.00 5000.00 Time High-throughput Cytological Discovery System CellVoyager CV8000
Transcript of High-throughput Cytological Discovery System · cells labeled with multi-fluorescent dyes. Two...
Yokogawa Technical Report English Edition Vol.60 No.2 (2017)
New Products
67 119
In the field of new drug discovery, the high content analysis (HCA) system is becoming popular for screening several hundred thousand compounds to identify promising candidates for new drugs. In HCA, cells are imaged with a microscopic camera, and their temporal changes are analyzed. To observe biological changes in living cells and identify the effect of the candidate compounds, it is necessary to observe living cells for a long time. Also, there is an increasing need to eliminate the effect of fluorescent labeling in cells and observe cell behavior without labeling, although intracellular functional molecules and organelles are generally labeled with fluorescent dyes for HCA analysis. However, automatic image analysis of label-free cells is challenging because their image contrast is lower than that of labeled cells. The CellVoyager CV8000 satisfies these latest needs.
MAJOR FEATURES
� Optimal cell-culture environment enabling long-term observation of living cells
The incubator mechanism of the system has been redesigned to improve the sealing of the incubator. As a result, cells can be cultured in a more uniform environment.
� Software for analyzing images of label-free cells and 3D-cultured cells
The CellVoyager CV8000 comes with CellPathfinder, which is new image analysis software that can recognize and analyze images of unlabeled cells with the help of pattern recognition and machine learning functions. This software also has a 3D image analysis function for observing 3D-cultured cells similar to those in living organisms.
� Quick imaging of multicolor-labeled cellsThe CV8000 can accommodate four cameras (three in the previous CellVoyager CV7000). The time required for imaging cells stained with four colors is reduced by more than 20% compared with the CV7000. The number of selectable laser wavelengths has been increased from four to five to offer wider options for experiments and various observations of cells labeled with multi-fluorescent dyes.
� Two Yokogawa Nipkow disks with industry-leading performance
The CellVoyager series uses Yokogawa’s original confocal scanning disk technology based on the Nipkow disk. The
conventional Yokogawa Nipkow disk with 50 µm pinhole diameter is suited for observing cells with weak fluorescence intensity. The CV8000 can also accommodate a disk with 25 µm pinhole diameter for capturing high-definition images especially with low-magnification objective lenses. Users can obtain clear images by using the two disks depending on the fluorescence intensity and the sample conditions.
MAJOR SPECIFICATIONS
● Optical systemConfocal unit: Microlens-enhanced wide-view Nipkow
disk confocal scannerExcitation laser wavelength: 405 nm, 445 nm, 488 nm,
561 nm, 640 nmTransmission-illumination source: LEDObjective lens: 2× to 60× (phase contrast, water immersion,
long working distance)Camera: High-sensitivity sCMOS camera (up to 4 units)
● Environment control (stage incubator)Temperature control, CO2 conc. control, forced humidification
● Built-in dispenser (option)Designated disposable chips needed
● Output data formatImage: TIFF, PNGNumerical data: CSV
APPLICATION EXAMPLE
● Measurement of calcium flux into cellsIonomycin of various concentrations was added to cells using the dispenser of the CV8000. Kinetic changes in calcium concentration in the cells are shown in the graph below.
Contact us:Life Science Center, Measurement Business Headquarters
TEL: +81-76-258-7032FAX: +81-76-258-7029
For worldwide locations, please see the back cover.* CellVoyager is a registered trademark of Yokogawa Electric Corporation.
CO2 incubatorCV8000
Top: Before incubationBottom: After 68 hours of incubation
CV8000 CO2 incubatorTotal area covered by cells at 68 hours of incubation (normalized by the area before incubation, average of 96 wells)
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Ionomycin was applied to A10 cells pre-incubated with Fluo-4, which were imaged at 100 ms intervals.
Mea
n in
tens
ity
Mean intensity/ionomycin conc. Ionomycin conc. (nM)
0.00 19.53 78.13 312.50 1250.00 5000.00
Time
High-throughput Cytological Discovery System
CellVoyager CV8000
