High Processing for seafood meat...
Transcript of High Processing for seafood meat...
High Pressure Processingfor seafood & meat productsRoman Buckow | Michelle Bull23 May 2012
CSIRO ANIMAL, FOOD AND HEALTH SCIENCES
Principles of high pressure processingprocessing
HPP seafood & meat | Roman Buckow2 |
Static High Pressure• Application of high pressures can cause:
• Inactivation of Parasites, Plant cellsV t ti i i
Features:
Why High Pressure Processing?
• Vegetative micro-organisms• Some fungal spores• Many food borne viruses• Enzymes are selectively inactivated• Macro molecules can change conformation• Small molecules (eg flavours) are generally unaffected• Food Systems – as above plus equilibria may change
• High pressure is instantaneously and uniformly applied to the sample
• Compression is fully reversible
Evolution of HPP industrial machines
120130140150160170
es in prod
uction
Oceania5% Asia
15%
Source: Source: HiperbaricHiperbaric
0102030405060708090100110120
Num
ber o
f HHP indu
strial machine
Europa25%
America55%
• Total machine number in production in December 2011 : 167
• Not included : 15 dismantled machines (all installed before 2003)
0
HPP users companies size profile
Very small <106%Group >5000
15%
(number of employees)Source: Source: HiperbaricHiperbaric
10<Small<5023%
50<Medium<25022%
250<Large<125022%
1250<Very Large<5000
12%
• 100 different HPP users companies in the world
• 51% are SME < 250 employees
Vegetable products
Meat products27%
Source: Source: HiperbaricHiperbaricHigh Pressure Processing of Foods
Juices and beverages
11%
32%Seafood and fish
16%
Other products14%
~100 Food Companies
~250.000 t/a Products in 2011
Most Applications:
Cold Pasteurisation in the final package
10000
100000
Pressure [MPa]
Center of Earth
Electrohydraulic Shock10000
100000
Pressure [MPa]
Center of Earth
Electrohydraulic Shock
What is the pressure we need?
10
100
1000
Mariana Trench
Piston Cylinder Press
Ultrasonics
Magnetic Compression
Hydraulic Systems
10
100
1000
Mariana Trench
Piston Cylinder Press
Ultrasonics
Magnetic Compression
Hydraulic Systems
295 (2002)
1
Atmospheric Pressure0.1
Thermal Autoclave1
Atmospheric Pressure0.1
Thermal Autoclave
250 MPa 600 MPaBefore After
Effect of pressure on micro organisms
High pressure can kill microorganisms by interrupting their cellular function without the use of heat that can damage the taste, texture, and nutrition of the food.
200 elephants weighing 3000 kg each standing on a piston with a
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High Pressure Processing
each standing on a piston with a diameter of a CD, create a static pressure of 600 MPa, 6000 bar or 90,000 PSI
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PistonSize CD
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HPP of food – early studies 1899
Hite 1899:
‐Pressure (600 MPa) li dapplied at room
temperature for 60 min can extend the shelf life of raw milk by 4 days
‐ Pressure also affects some native milk enzymes and proteins
‐ Some Coagulation of fat and proteins under pressure
Commercial application of HPP – pressure range
CSIRO.
Volume contraction and compression heating
120
140
WaterPropylene-GlycolHDPEPPPTFE
20
40
60
80
100
Tem
pera
ture
/ °C
0 100 200 300 400 500 600 7000
Pressure / MPa
Compression heating of water, propylene-glycol, HDPE (High density polyethylene), PP (Polypropylene) and PTFE (Polytetrafluoroethylene) at Tini = 10, 50, 90°C.
Compressibility of water and carbon dioxide at 20°C.
Δ H = - 68.2 kJ/kg
ρ = 1.165 kg/L tetrag.
Ice modifications under pressure
Δ H = - 19.0 kJ/kg
ICE III
ICE II
ρ = 1.170 kg/L rhomb.
Δ H = - 42.5 kJ/kg
ICE I
ICE II ρ = 0.920 kg/L hex.
Pressure Temperature Diagram of Inactivation
5 log10 reduction in 60 s
HPP industrial equipment• Indirect compression system
• Horizontal design for traceability and easy installation
• Special design for food industry (stainless steel, cleanable…)
A i l di / l di• Automatic loading / unloading system
• Working pressure up to 600 MPa (6000 bar)
• From 200 kg up to 2 tons/hours
Industrial HPP machines
420 L vessel
160 L vessel
Avure Technologies
350 L vessel
HPP equipment manufacturer
HIPERBARIC AVURE MULTIVAC KOBELCO CHIC
Country Spain USA Germany Japan China
Hiperbaric, S.A.Avure
MULTIVAC Kobe SteelShinko Building,
CHIC FresherTech Global Sales
Address
C/ Condado de Treviño, 6Pol. De Villalonquejar –09001 Burgos, Spain
AvureTechnologies Inc 22408 66th Avenue SouthKent, WA 98032, U.S.A.
Australia PTY. LTD.2 McGregorsDrive3042 TullamarineAustralia
10‐26, Wakinohamacho 2‐chome, Chuo‐ku, KobeHyogo 651‐8585Japan
Global Sales19th Floor, GangTai PlazaNo 700 East YanAn RoadShanghai 200001P.R. China
Phone +34 947473874 +1 253 981 6350 +61 3 8331 2800+81 78 261 5111
+86 21 5385 0085
Fax +34 947473531 +1 253 981 6230 +61 3 8331 2810+81 78 261 4123
+86 21 5385 0005
Emailinfo@ hiperbaric.com
[email protected]@ multivac.com.au
info@ kobelco.co.jp
freshertech@ chicgroup.cn
Website hiperbaric.com avure.com multivac.com kobelco.co.jpchicfreshertech. Com
Industrial HPP systems in operation
HPP systems at CSIRO
•2 mL kinetic, (700 MPa) and temp
•300 mL, pressure (800 MPa) and temp
•2 L, pressure (500 MPa)
( ) d•3L, pressure (800 MPa) and temp
•35L, pressure (690 MPa) and temp2ml kinetic
35 L Avure Technologies3L Stansted
Recent CSIRO HPP publications
High pressure processing | Roman Buckow | Page 22
Microbiological safety and stability of HPP productsstability of HPP products
HPP Microbiology | Michelle Bull 23 |
Risk Management: Finding the Balance
Need to destroymicroorganisms vs
Optimise:Flavour, texture, colour,nutritional quality
2
Innovative Processing
Traditional Processing
HPP Microbiology | Michelle Bull
Spoilage Microorganismse.g. Yeasts, moulds
Lactobacilli
Delay GrowthPrevent GrowthInactivate
Defining the microbial hazard
Lactobacilli
Toxigenic pathogense.g. S. aureus, Cl. botulinum
Infectious pathogens
Prevent GrowthInactivate
Inactivate
p geg Listeria, Salmonella,
E. coli O157Inactivate
The hurdle concept
The microbiology of food is determined by the microbial, processing, intrinsic and extrinsic factors
heat acid salt chill
E h bi ti f h dl d fi th
figure adapted from Leistner & Gould, 2002
Each combination of hurdles defines the microbiological safety & stability of a food
The hurdle concept
By adding new hurdles, the relative level or concentration of existing (less preferred) hurdles may be reduced
HPPheat acid salt chill
Opportunities for product improvement & new product development
figure adapted from Leistner & Gould, 2002
Microbial inactivation by HPP
Lethal effects on microorganisms may include
denaturation of proteins & enzymes
changes in the permeability of membranes = leakage & c a ges t e pe eab ty o e b a es ea age &susceptibility to other hurdles
Differences in sensitivity to HPP
Gram negatives > Yeast/Mould > Gram positives > Spores (only with heat also) “rule of thumb”
Product specific: inactivation dependent on pH, water activity, salt concentration, etc.
+HPP
y, ,
Process specific: exposure time, pressure level, etc.
CFNS| Michelle Bull
2
Listeria monocytogenes after 180s at 600MPaCapitalised on for e.g. alternatives to USDA
FSIS Interim Final Rule re: RTE meats (2003)
High pressure processing
Ambient temperature
moulds
Chi
ll st
able
Am
bient stable
vegetative pathogens
viruses
non-proteolytic proteolytic
moulds
various
vegetative pathogens
CFNS| Michelle Bull
2
e
High temperature
C. botulinum C. botulinum
Bacterial inactivation in different P / T domains
C. jejuni: Chicken meat slurry,Lori et al. (2007)
E. coli: 10 mM Hepes-buffer, pH 5.3,Sonoike et al. (1992)
L. monocytogenes: Prosciutto ham slurry,Buckow et al. (unpublished)
L. casei: Hepes-buffer, pH 5.3,Sonoike et al. (1992)
Z b ilii M t t l
Presentation title | Presenter name
3
Z. bailii: Metwurst slurry,Lori et al. (unpublished)
Virus inactivation in different P / T domains
Avian Influenza Virus: Chicken meat slurry,
( )Isbarn et al. (2007)
Feline Calicivirus: Cell culture medium,Buckow et al. (2008)
Coxackie Virus B5: Cell culture medium,Buckow et al. (submitted)
Rotavirus A: Cell culture medium
Presentation title | Presenter name
3
Rotavirus A: Cell culture medium,Isbarn et al. (submitted)
400 MPa/1 min/20oC 600 MPa/10 min/20oC
Effect of high pressure & storage temperatures on the microbiological quality of cooked poultry meat
Presentation title | Presenter name
3
(Patterson, 2011)
Improved safety through HPPHPP RTE meat Inactivation of Listeria monocytogenes
NaCl
Number of samples positive for growth*
ProductNaCl
(% wt/wt)
p gafter treatment at 600
MPa for210 s 300 s
Chicken 2.1 0/5 0/5Shaved Chicken Breast 4.8 5/5 0/5
Turkey 1 9 0/5 0/5
CFNS| Michelle Bull
3
Turkey 1.9 0/5 0/5*via enrichment: limit of detection greater than 1 CFU in a 25g sample (USDA), inoculum conc. ~104 cfu/g
Youart et al. (2010)
‘no preservatives’
‘salt-reduced’
‘chemical-free’
In‐pack pasteurisation by HPP
Ready-to-eat meats processed at 600MPa for 180sExtension of refrigerated shelf-life from 45-50 days to >98 daysdays to >98 daysApprox. 4-log reduction of L. mono.
CFU
/ml
1 0 2
1 0 3
1 0 4
1 0 5
1 0 6
1 0 7
24 72 26 55 26 57 23 45 23 40 23 43 25 42 23 41 23 42
Hayman et al. 2004
T im e (s)
0 1 0 20 30 4 0 50 60 7 01 0 0
1 0 1
1 0 2
ProductShelf‐life extension
Other quality benefits or
deleterious effectsReference
Shelf‐life extension by HPP – meat products
Shelf-life extension by HPP – meat products
Marinated beef loin (raw)
13‐15 days Some greying of meat Garriga et al. (2004)
Blood sausage (fresh)
28 days None mentioned Diez et al. (2008)
Cooked sliced hamup to 66 days depending on HPP level
No deleterious effects on quality
Slongo et al. (2009)
Improved safety with HPP – seafood
Product Microbial hazard HPP Reference
Live oysters V. parahaemolyticus5‐log10 reduction≥ 350 MPa / 2 min
(1 – 35°C)Kural et al. (2008)
Oysters Hepatitis A1.6-log10 reduction425 MPa / 1 min
Grove et al. (2009)
Oysters Hepatitis A2.2-log10 reduction475 MPa / 2.5 min
Grove et al. (2009)
Shelf‐life extension by HPP – seafood
Product Shelf‐life extensionOther quality benefits or
deleterious effectsReference
Atlantic salmon (fresh)2 days with HPP alone, 6 days with HPP and MA
Colour effects and rancidity for HPP + MA with longer storage
Amanatidou et al. (2000)
Tuna
(minced)
Alternative to canning; >22 d chilled and >93 d
frozen
Protection from lipid oxidation, some colour
change
Ramirez‐Saurez & Morrissey (2006)
Oysters up to 21 days depending Increased cutting Cruz‐Romero et al. y
(live)p y p g
on HPP level g
strength with HPP (2008)
Salmon
(cold smoked)22 days
Little change in odour, some colour change
Medina et al. (2009)
Applications & opportunities
HPP seafood & meat | Roman Buckow38 |
Effects of high pressure on proteins
Effects on covalent
Molten globuleMonomerOligomer Unfolded
Atmospheric pressure
1-2 kbar3-5 kbar
>3 kbar >30 kbar
covalent bonds
Aggregation
CSIRO.
Scheme of the elliptic phase diagram of proteins. The arrows marked by the letters p, h, c show the specific denaturation ways known as pressure, heat and cold denaturation.
25°C
Ref. 100 200 300 400 500 MPa
Effect of high pressure on muscular structuresTurkey breast (M. pectoralis superficialis) after 1 min at 0.1-500 MPa and 25°C.
Ref. 100 200 300 400 500 600 MPa
25°CChicken breast (M. pectoralis superficialis) after 1 min at 0.1-600 MPa and 25°C.
Pork meat (M. longissimus dorsi) after 1 min at 0.1-600 MPa and 25°C.
Ref. 100 200 300 400 500 600 MPa
25°C
Ref. 0.5 s 15 s 30 s
Colour change in minced meat
Minced Meat
e 0 5 s
0.5 s 10 s 20 s 30 s
10 s 20 s 30 s0 5 s 10 s 20 s 30 s0.5 s
Inactivation of E.coli in fresh fermented sausage
-1
0 E. coli30 °C
-5
-4
-3
-2
1
Log
Red
u ctio
n [-
]
5000 bar
-6
5
0 20 40 60 80 100 120Time [s]
6000 bar
HPP Commercial Meat Products• Shelf life increase
• Destruction of pathogens : Listeria, Salmonella, coliforms…
• Stabilisation of preservative‐free or low salt content products Country Year Products Spain 1998 Sliced cooked ham and “tapas”USA 2001 Sliced cooked products and proscuitto ham USA 2001 Poultry products USA 2002 Pre‐cooked chicken and beef strips Spain 2002 Sliced cooked meats products, Serrano cured ham Italy 2003 Proscuitto ham, salami & pancetta Germany 2004 Cured and smoked sliced and diced ham Japan 2004 Nitrites‐free bacon, sausages and sliced meat USA 2005 Ready‐to‐eat meat based products Spain 2005 Cured meat products & Serrano hamCanada 2006 Cured & cooked meat products USA 2006 Whole roasted chicken USA 2006 Sliced cooked turkey and chickenCanada 2006 Ready‐to‐eat meat mealsUSA 2007 Chicken sausages USA 2008 Cooked pork & beef sliced productsUSA 2008 Pet foodCanada 2008 Sausages and baconCanada 2009 German style cooked meat products USA 2009 Sliced RTE meatsCanada 2010 Proscuitto ham and cured meats Australia 2010 Sliced and diced preservative free poultry products Switzerland 2011 Cooked pork sliced products and sausagesUSA 2011 Proscuitto ham and cured meats USA 2011 RTE sliced meatsRumania 2011 RTE pork productsSpain 2011 Serrano ham and cured meats
Why does the meat industry use HPP ?
• Non‐ thermal post packaging pasteurization (approved by FSIS in USA)
• Safest alternative for sliced meat products
iency
• Significant shelf life extension
• No size / volume effect : whole, sliced, stripped & diced meat products
• Suitable for vacuum‐packed or MAP
Effic
Total aerobic microflora
468
FU /
g)
Coliforms
468
FC /
g)
Lactic acid bacteria
468
UFC
/
)
024
0 20 40Storing days at + 6º C
Log
(CF
024
0 20 40Storing days at + 6ºC
Log
(U
024
0 20 40Storing days at + 6º C
Log
(U g
HPP treatment of sliced cooked ham at 500 MPa for 8 min at + 8ºC
Why does the meat industry use HPP ?
• No effect on sensorial quality
• Keep fresh tasteity
Keep fresh taste
• Maintain nutritional value
• Remove chemical preservatives
Qua
li
5
10Colour
SlicedFibrousNon processed
HPP treatment of sliced cooked ham at 500 MPa for 8 min at + 8ºC
0 Adhesiveness
Crunchy
Elasticity
Tenderness
JuicyHPP
Espuña ‐ Spain
• for sanitization of ready‐to‐eat meals and cured meat products
Campofrío ‐ Spain• sliced cooked ham and boneless cured ham (Export toUSA of cured ham “Listeria‐free”)
• “Vuelta y Vuelta” range: gross slices of ham, turkey, and chicken (meat taste, less additives, longer shelf life: 8 weeks): 8 weeks)
• “Sanissimo” (Super Healthy !) : cooked ham withOmega 3 & no added salt
• “Vuelta y Vuelta” marinated chicken and turkey ready‐to eat products
Infantis Freshpress ‐ Greecewww.freshpress.gr
Hormel ‐ USA• HPP dry cured ham in the US market since 2001
• Large range of HPP sliced RTE ham, turkey and beef
• Vacuum‐packed and MAP
• Label “True Taste Technology”• Label True Taste Technology
• Info on HPP in the web site and flyer
Hormel ‐ USA
http://www.hormelnatural.com/
• sliced RTE preservative free meats
Kraft Foods ‐ USA
Maple Leaf ‐ Canda• RTE preservative free meals and salads
CSIRO.
• Shelf life extention of preservative‐free steak tartare
Zwanenberg ‐ The Netherlands
• preservative‐free RTE meals and sausages
MRM ‐ Spain
FressureTM beef patties
Cargill ‐ USA
• Shelf life of 21 days vs 10 days of the non‐HPP equivalentP d• Patented process
Moira Mac’s Fine Food ‐ Australia
HPP Commercial Seafood Products•Shelf life increase
•Shucking bivalves
•Easy shellfish extraction
•Destruction of Vibrio Country Year ProductsUSA 1999 OystersUSA 2001 OystersUSA 2001 OystersUSA 2001 OystersUSA 2003 CrabCanada 2004 SeafoodCanada 2004 LobstersN. Zealand 2004 Mussels shucking for medsItaly 2004 Desalted codUSA 2005 LobstersUSA 2005 LobstersCanada 2006 SeafoodJapan 2007 ShellfishUSA 2008 CrabSpain 2009 RTE fish mealsUSA 2010 OystersCanada 2010 LobstersNew Zealand 2010 Green musselsFrance 2011 Losters & clams
Effect of HPP on appearance of seafood
High pressure treatment of oysters• Usually eaten raw or lightly cooked• Potential for pathogenic bacteria• Different bacteria and spoilagemechanisms (high glycogen)
HPP• Shucking• Appearance improves• Increased moisture retention• Bacterial inactivation• Extended shelf life• Change in microflora ‐ packagingChange in microflora packaging• Lipase increases ~ 7d
• extraction of raw lobster meat
Ocean Choice ‐ Canada
• clams shucking
Mitsunori ‐ Japan
http://www.youtube.com/watch?v=OYSbc1_l7tM
HPP Packaging & Costs
HPP seafood & meat | Roman Buckow66 |
Packaging requirements• Flexible packaging (>15%volume contraction)
• Extra tight seals• Rounded & reinforced edges • Minimal head spaceMinimal head space• MAP possible• Tear strength, puncture
resistance, surface smoothness generally not affected by HPP
• Often flexible pouches or bottles are inserted in secondary cardboard containers or sleaves after processing
Delamination of packaging film
Conventional HPP processing of typical MAP products
Damages could occur when insufficient packaging and
processing is used
Micro defects(„white spots“)
Bending and delaminationof packaging film
In particular modified atmosphere packs
(MAP) have been critical.
Micro defects(„white spots“)
Source: Tobias Richter, Multivac
Examples for a suitable HPP packaging concept
• optimal filling degree
• perfect package solution
(film thickness, form, depth)
no delamination
d dno product damage
no white spots
High pressure treatment: 600 MPa for 3 minSource: Tobias Richter, Multivac
Optimise packaging
Poor Design Better Design Best Design
HPP packaging and presentation formats
Days of production / a 300 300 dHours / d 16 16 h/d
Process pressure 600 600 MPaStarting temperature 5 90 °C
Vessel size 325 125 LNumber of vessels 1 1 U
Cycle time 8.0 8.0 minCompression time 2.5 2.5 min
Filling degree 60 60 %Power (compression) required 292.68 123.82 kW
Capacity per cycle 0.195 0.075 m³/CycleCycles / a 36,000 36,000 1/a
Capacity / h 1.463 0.563 m³/hPacks / h 7,313 2,813 units/h
Production / a 7,020 2,700 m³/aPacks / a 35 100 13 500 1000/a
Processing Costs
• High Pressure Low Temperature (blue):
– 600 MPa, 5 min,
– Tini = 5°C
V l 325 L Packs / a 35,100 13,500 1000/aCycles / d 120.0 120.0 1/d
Electric power 63 69 kWh/m³ productWater 0.765 0.765 m³/m³ product
Facility 1,896,120 1,738,110 USDExtra pumps (if required) 260,717 284,418 USD
Accessories 27,968 30,180 USDInstallation / Commissioning 94,806 94,806 USD
Duty 22,796 21,475 USDFreight 31,602 31,602 USD
Electric power 0.05 0.05 USD/kWhWater 1.00 1.00 USD/m³
Maintenance 6.48 10.8 % of total installation costs per yearInsurances 0.5 0.5 % of total installation costs per yearOther costs 5 5 % of total cost per year
Depreciation period 5 5 aInterest rate per period 7 7 %
Engineer(s) 0.10 0.10 per shift
– Vessel = 325 L
• High Pressure Thermal Sterilisation (orange):
– 600 MPa, 5 min,
– Tini = 90°C
– Vessel = 125 LEngineer(s) 0.10 0.10 per shift
Worker(s) 2.00 2.00 per shiftEngineer costs/a 60000 60000 USD/a
Worker cost/a 40000 40000 USD/aNumber of shifts 2.00 2.00
Overall number of workers 4.2 4.2Electric power 23,989 12,945 USD/a
Water 5,371 2,066 USD/aSpare parts 141,575 221,692 USD/a
Insurance 10,924 10,264 USD/aManning costs 172,000 172,000 USD/a
Depreciation charge 569,243 536,704 USD/aMiscellaneous costs 46,155 43,484 USD/a
Sum 969,257 1,000,436 USD/aCost per m³ product: 138.07 371.65 USD/m³
Cost per tonne product: 131.50 353.95 USD/tCost per cycle: 26.92 27.87 USD/cycle
Disclaimer: Processing costs may vary greatly!
HPP Energy Costs• Major energy consumption during compression phase
• No significant difference between pressure increase at low (e.g. 5°C) or high (e.g. 90°C) temperatures
• Linear increase of energy consumption with pressure and volume due
Sensitivity Analysis
Sensitivity Analysis
?
?
Conclusions
• Generally, the process costs for High Pressure Thermal Sterilisation are considerably higher than those for High Pressure Low Temperature processing
• The costs for HPLT and HPTS for 600 MPa/3min process can be estimated to be 0.15 and 0.25 AUD per kg product, respectively
• Sensitivity analysis: capital cost, filling degree efficiency, production hours, depreciation period, and cycle time greatly affected the processing costs
• Manning and energy costs only had a relative minor impact on the process cost.
ConclusionsConsumers: HPP is a consumer acceptable, environmental friendly, scientifically recognised method to achieve higher quality in certain foods
Processing: Pressure transmission is instantaneous and uniform (not heat transfer controlled, no ‘shadow’, depth, or uneven distribution effect)‐ rapid, short processing times, assured safety in whole pack, suitable for solids and liquids
Quality: retains flavour and nutrition
Environmentally: safe and no process by‐products, no emissions
Packaging: Package design, geometry and format should be tailored for HPP, Packaging films and laminate structure generally survive HPP well, but MAP and HPP at high temperature can cause delamination and defectsOTR and WVTR can be affected by HPP
International Nonthermal Food Processing Workshop ‐ FIESTA 2012
16‐17 October 2012, Melbourne Australia
Innovative processes for sustainable safe and healthy foodsInnovative processes for sustainable, safe and healthy foods
www.innovativefoods2012.com
Poster abstracts close July 15
Supported by the Institute of Food Technology’s Nonthermal Processing Division, the European Federation of Food Science and Technology
and the Australian Institute of Food Science and Technology.Presented by the Food Innovation Emerging Science and Technology ApplicationsPresented by the Food Innovation Emerging Science and Technology Applications
(FIESTA) Conference series.
Thank youProcess Engineering ScienceRoman BuckowResearch Group Leader
t +61 3 9731 3270
Food MicrobiologyMichelle BullStream Leader
t +61 3 9731 3270t +61 3 9731 3270e [email protected] www.csiro.au/fns
CSIRO FOOD AND NUTRITIONAL SCIENCES
t +61 3 9731 3270e [email protected] www.csiro.au/fns