HERITAGE PROJECT #

HERITAGE PROJECT TITLE PROJECT PROPONENT Total Award FY 12 Expended FY 13 Expended BALANCE REASON FOR EXTENSION REQUEST

12-30Population genetic diversity and connectivity in Nevada's mule deer.

UNR (Marjorie Matocq) $ 30,000.00 $ - $ 6,627.43 $ 23,372.57

Experienced considerable delay in obtaining all necessary samples to complete our portion of the work; we are making excellent progress, given the breadth of collaborations project entails, we should have requested a longer project period from the start

12-36 Investigation of cause-specific mortality in Nevada mule deer

UNR (Kelly Stewart Cody Schroeder)

$ 25,139.58 $ - $ 2,049.17 $ 23,090.41 Funding through university not made available to spend until March 13, 2013

13-03Pine Nut Vegetation and Sage-Grouse Research Project (bi-state population)

Mark Freese (NDOW) $ 40,000.00 $ 22,290.00 $ 17,710.00

Collars to be placed on sage-grouse during the spring of 2013; however, sage-grouse were not found on the only known active lek within the Pine Nuts, data shows birds have relocated south near the Bodie Hills. BLM is currently soliciting public comments, expecting a decision in September 2013. Remaining balance to be applied to data acquisition and habitat improvement.

13-04 Overland Bigwash Caleb McAdoo (NDOW) $ 65,000.00 $ 65,000.00 Unforeseen permitting delays, currently in the NEPA process , anticipate a record of decision later this year which will allow us to proceed with the project.

13-05 Spruce Mountain Alan Jenne (NDOW) $ 20,000.00 $ - $ 20,000.00 Implementation of the project has been delayed due to NEPA appeals which were only recently decided in the project's favor.

13-06Disease Diagnostics and Continued Disease Surveillance for Big Game in Nevada

Peregine Wolff (NDOW) $ 30,000.00 $ 11,256.96 $ 18,743.04 To continue disease surveillance and establishment of base line information on the health of Nevada’s game species

13-07 Wildlife Health Database Peregine Wolff (NDOW) $ 13,000.00 $ - $ 13,000.00

Currently using the database. Will need time to complete the contract process with CCWHC (Canada) and Oregon Department of Fish and Wildlife in order to make payment.

13-08 Duck Creek Valley Sage Grouse Habitat Improvement Project

Lee Turner (NDOW) $ 35,000.00 $ - $ 35,000.00 Project is delayed due to NEPA work being sidetracked during last fall's busy fire season

13-12 Bear DNA Sampling and DispersalCarl Lackey (NDOW) & Marjorie Matocq (UNR)

$ 40,000.00 $ 3,006.26 $ 36,993.74

Project is on track. Requesting more time, recently established collaboration with California, hired personnel, started purchasing supplies. Rapid progress is expected this summer and early fall.

13-20Investigation of trace mineral levels and forage quality of Nevada mule deer

Cody Schroeder (UNR) $ 20,000.00 $ - $ 20,000.00 Funding through university not made available to spend until March 13, 2013

HERITAGE PROJECT EXTENSION REQUESTS for JUNE 2013 CONSIDERATION

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Dear Heritage Committee: Please accept this email as a request for extension on our Heritage project #12-30 concerning population genetic structure of mule deer in Nevada. As detailed in the attached update progress report, we experienced considerable delay in obtaining all necessary samples to complete our portion of the work. In hindsight, given the breadth of collaborations that this project entails, we should have requested a longer project period from the very start. Regardless, we are making excellent progress on this research and look forward to sharing more results with you soon. I am aware of the Heritage extension request meeting. Unfortunately, because of other commitments, I will not be able to attend to answer questions that may arise in the meeting. However, I will be available by phone (you have my contact information) or could answer any questions that arise in advance upon initial review of this request. Please let me know if there is any additional information I can provide to facilitate your favorable review of our request. Thank you in advance for your time- Marjorie _____________ Marjorie Matocq Associate Professor Department of Natural Resources and Environmental Science Program in Ecology, Evolution, and Conservation Biology University of Nevada Reno Reno, Nevada 89557 775-784-4621

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Progress Report Heritage Award #12-30

Population genetic diversity and migration in Nevada’s mule deer Prepared by Dr. Marjorie Matocq

University of Nevada, Reno June 4, 2013

Associated with our request for a no-cost extension on this award, we would

like to take this opportunity to share our progress on this research. This project represents a strong collaboration between the University of Nevada, Reno, the Nevada Department of Wildlife, the sportsmen of Nevada and collaborators in California. Because amassing the samples for this research required close coordination across all of these groups, delays were experienced. Nonetheless, as of Spring 2013, adequate sampling is now in hand to begin a thorough investigation of genetic diversity in mule deer across the State of Nevada. Here, we share some of our earliest insights. Given that this report will be quickly outdated, we are happy to update these findings at the time of their review. Sampling distribution. The Nevada Department of Wildlife has generously shared blood samples from their extensive, ongoing direct handling of mule deer across the State. To augment our view of genetic resources across the State, and not just limited to the focal areas of intensive NDOW study, we turned to the sportsmen of Nevada. Through their efforts and additional assistance from several taxidermists, we now have quite extensive coverage from key areas (Figure 1). My laboratory has now catalogued a total of 901 samples. As shown in Figure 1, the areas of highest sampling density are those associated with ongoing ecological and behavioral research, augmenting the importance of the genetic data that we will generate from these. However, even from the localities with fewer samples, in combination, these provide a broad geographic perspective on the overall distribution of genetic resources .

Figure 1. Spatial distribution of 901 mule deer samples now available to this project.

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Central to our efforts of establishing patterns of genetic diversity and genetic connectivity within Nevada, is the need to place these patterns in a broader geographic context. To achieve this goal, we are working closely with the laboratory of Dr. Robert Wayne at the University of California, Los Angeles. Dr. Wayne’s group recently completed a thorough investigation of genetic structure of deer in California and has generously agreed to share samples with us so that we can place Nevada’s diversity within a larger western view of this taxon. With this effort, we will have a clear view of how much gene flow is occurring across state boundaries and, in particular, assessing any possibility of genetic exchange between mule deer and black-tailed deer. Mitochondrial DNA. Variation in mitochondrial DNA (mtDNA) has long been used as a measure of genetic diversity. Latch et al. (2009) conducted a broad survey of mtDNA variation in mule deer and black-tailed deer across North America and included two sampling locations from within Nevada. Their work discovered 14 mitochondrial haplotypes in Nevada. To date, we have obtained sequence data from the same portion of the mtDNA control region as Latch et al. (2009) for 62 deer. We discovered 14 of the same haplotypes as Latch et al. (2009) but also an additional 7 haplotypes unique to Nevada. The 21 haplotypes that exist in Nevada form two distinct evolutionary lineages (haplogroups or networks; labeled orange and a combined blue/green to be consistent with Latch et al. 2009; Figure 2).

Our geographic range of sampling also extends our understanding of the spatial distribution of mtDNA in Nevada. Both mtDNA lineages are spatially intermixed and appear to be represented throughout the State, although the green/blue lineage appears to exist in higher frequency and additional sample processing from the central portion of the state is needed to confirm the presence of the orange lineage in this area.

Figure 2. Spatial distribution of two distinct mitochondrial DNA lineages within Nevada.

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Microsatellite variation. While mtDNA variation is an important index of diversity, especially in efforts to establish historic patterns of colonization and movement, resolution of ongoing movement and finer spatial scale processes can only be elucidated by hyper-variable regions of the genome such as nuclear microsatellites. Our efforts to date have focused on 9 microsatellite loci, although we have recently completed optimization of an additional 14 loci. The initial loci, genotyped for a total of 79 deer thus far, are moderately variable with a mean of 6.4 alleles per locus (range 3-13) and a mean heterozygosity of 0.63 (range 0.48-0.89). Our initial analysis based on principle coordinates does not identify distinct genetic groups (Figure 3). Rather, at this initial stage, there appears to be genetic continuity at a broad spatial scale, even with respect to the mitochondrial lineages that exist in Nevada (Figure 3). Further sample processing and augmenting our number of loci are necessary before solid conclusions can be reached concerning spatial structure or lack thereof.

Ongoing work. We have now optimized conditions on an additional 14 loci, so we are poised to generate data for a total of 25 loci. This summer and fall, we will genotype additional samples to address both broad and regional scale patterns of variation and connectivity in this system.

Coor

d. 2

Coord. 1

Principal Coordinates

1

2

Figure 3. Principle coordinates analysis of nuclear microsatellite variation at 9 loci in mule deer from Nevada. Colors refer to the mtDNA haplotype lineage of that individual as reflected in Figure 2.

HERITAGE PROJECT #12-36 EXTENSION REQUEST Dear Heritage Project Committee, I would like to request an extension for proposal #12-36 Cause specific mortality in mule deer, authors are myself and Cody Schroeder. We did not receive the funding for this grant until March 13, 2013 we were unable to spend out the grant by the July 1 deadline. I will attend the Commission meeting on June 21, to testify regarding our needs for extending this grant. Thanks, Kelley Stewart Large Mammal Ecologist Assistant Professor University of Nevada Reno 1664 N. Virginia St / Mail Stop 186 Reno, NV 89557 775-784-4314 775-784-4583 (fax) 775-313-5280 (cell)

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STATE OF NEVADA DEPARTMENT OF WILDLIFE

1100 Valley Road · Reno, Nevada 89512

(775) 688-1500 Fax (775) 688-1595

MEMORANDUM June 17, 2013 To: Heritage Commitee Cc: Shawn Espinosa, Steven Siegel From: Mark Freese Subject: Pine Nut Vegetation and Sage-grouse Research Heritage Project #13-03 I am writing to request an extension of unused Heritage funds for the Pine Nut Vegetation and Sage-grouse Research Project. To date, approximately $22,290 has been spent purchasing GPS collar hardware. Of the $17,710 remaining to date, approximately $8,500 dollars will be used for the data acquisition software to obtain collaring data from the collars. The remaining balance of $9,210 will be applied towards the habitat improvement work as described in the original proposal. Collars were going to be placed on sage-grouse during the spring of 2013; however, sage-grouse were not found on the only known active lek within the Pine Nuts. Based upon data from some collared birds, many of the Pine Nut birds have relocated south near the Bodie Hills. Currently, NDOW is reassessing our approach. It is likely that collars will go toward birds elsewhere in the Bi-State Sage-grouse population such as on Mt. Grant where collaring information is needed and a high prioirty. The BLM is currently soliciting public comments on the “Pine Nut Land Health Project” and expecting a decision in September 2013. Once a decision is complete, habitat improvement work will commence. The “Pine Nut Land Health Project” evaluates and prioritizes habitat improvement projects in the Pine Nut Mountains for the benefit of sage-grouse. I am requesting an extension on unused funds through June 30, 2014 to allow for the establishment of appropriate funding mechanisms (e.g. subgrant) to be established and provide flexibility with the collaring component of the project.

HERITAGE #13-04 EXTENSION REQUEST

On Jun 4, 2013 2:33 PM, "Caleb McAdoo" <cmcadoo@ndow.org> wrote: ------------------------------------------- From: Caleb McAdoo[SMTP:CALEB.MCADOO@GMAIL.COM] Sent: Tuesday, June 04, 2013 2:33:01 PM To: Caleb McAdoo Subject: Re: FW: HERITAGE EXTENSION REQUESTS Auto forwarded by a Rule

I request an extension for the Overland Project13-04. Due to unforseen permitting delays, we were unable to utilize the funding this year. We are still currently in the NEPA process , but anticipate a record of decision later this year which will allow us to proceed with the project.

Thank you,

Caleb

On Jun 4, 2013 12:50 PM, "Caleb McAdoo" <cmcadoo@ndow.org> wrote:

Heritage Project Funding Extension Request

Project: Spruce Mountain Heritage Project #13-05 Project Manager: Alan Jenne Eastern Region Supervising Habitat Biologist 775-777-2306 ajenne@ndow.org Awarded Funding: $20,000.00 Unspent Balance: $20,000.00 Carry Forward Request: $20,000.00 Justification For Request: Implementation of the project has been delayed due to NEPA appeals which were only recently decided in the Projects favor. These delays did not allow ample time to schedule the treatment within FY-13. However, it is fully expected that treatment will commence during late summer or early fall of FY-14 and all Heritage funds will be expended.

Request for extension of Heritage Grant # 13 – 06

Disease Diagnostics and Continued Disease Surveillance for Big Game in Nevada

Award amount: $30,000

Amount spent: $approx. $15,000

This funding was used to support continued disease surveillance in our mule deer herds sampled across western, central and eastern Nevada. Funding also supported collaborative ongoing work in mule deer, bighorn sheep and mountain goats to fully understand if there are population level impacts on these three species in Area 10 where there is a high prevalence of exposure and transient infection with Bovine Virus Diarrhea Virus. Monies were also used to support sampling of additional bighorn sheep and mountain goats in the East Humboldt’s and Ruby Mountains ecosystem to determine the continued prevalence of Mycoplasma ovipneumoniae and pathogenic Pasteurella sps. which have documented population limiting effects and thus management implications on these two species. Funding was also spent on diagnostics for individual dead wildlife including deer, elk, mtn. lions, bears and upland game bird species. Of particular interest was the identification of Pigeon paramyxovirus -1 in Eurasian-collared doves in Las Vegas. This virus has been recognized in a number of nearby states including AZ and CA and has caused localized mortality in Eurasian-collared doves. There have been reports that native dove species (white winged doves) can be infected. To date NV has only confirmed the virus in Eurasian-collared doves. I realize that doves are not a big game species however there was much concern by Nevada’s dove hunters on the impact of this virus on dove species.

I am requesting an extension of this grant to continue disease surveillance and establishment of base line information on the health of Nevada’s game species.

Request for extension of Heritage Grant # 13 – 07 - Wildlife Health Database

AWARD AMOUNT $13,000

Amount spent $0.00

The original agreement for the purchase of the database was that the Oregon Department of Fish and Wildlife (ODFW) and the Nevada Department of Wildlife (NDOW) would split the original licensing cost as well as the annual maintenance fee. The original plan would be that ODFW working with CCWHC through an MOU would pay these costs and that NDOW would reimburse ODFW. In January 2013, ODFW inquired as to whether NDOW could work directly with CCWHC to pay for our half of the licensing fee and annual maintenance fee. Due to a number of factors involving the contract approval process, NDOW and CCWHC were unable to sign a contract agreement before June 30th. At this time, ODFW has purchased the license for database at full cost. NDOW will continue to work on an agreement with ODFW and CCHWC to fund our ½ of the database and annual fee.

Both NDOW and ODFW are currently authorized to begin using the database and training sessions with CCWHC on data entry have been conducted. Funding that was earmarked for this project that is non-renewable is currently being used to support a temporary hire to conduct data entry.

I am requesting an extension of this project so that we have the time to complete the contract process with CCWHC to support NDOW’s cost for the database and annual licensing fee.

Heritage Committee: I would like an extension on Project # 13-08, Duck Creek Valley Sage Grouse Project. Project is delayed due to NEPA work being sidetracked during last fall's busy fire season. Ely BLM is finishing that NEPA work now and should be done by Sep 2013.

Lee Turner, PhD Nevada Department of Wildlife Office: 775-688-1542 leeturner@ndow.org

Dear Heritage Committee: Please accept this email as a request for extension on our Heritage project #13-12 concerning population genetic structure of bears in Nevada. We are progressing well on our anticipated two-year timeframe for this research. To date we have assembled and catalogued all bear samples recently collected in Nevada (courtesy C. Lackey), coordinated to have older samples returned from a testing lab in Colorado, and established a collaboration with a laboratory in California to obtain representative samples of the genetic groups recently discovered in California. We have hired the personnel for this project and started purchasing necessary reagents to allow rapid progress in this work over the summer and early fall. I am aware of the Heritage extension request meeting. Unfortunately, because of other commitments, I will not be able to attend to answer questions that may arise in the meeting. However, I will be available by phone (you have my contact information) or could answer any questions that arise in advance upon initial review of this request. Please let me know if there is any additional information I can provide to facilitate your favorable review of our request. Thank you in advance for your time- Marjorie _____________ Marjorie Matocq Associate Professor Department of Natural Resources and Environmental Science Program in Ecology, Evolution, and Conservation Biology University of Nevada Reno Reno, Nevada 89557 775-784-4621

Dept. of Natural Resources & Environmental Science 1664 N. Virginia Street / 330

Reno, Nevada 89557 (775)784-4020 office

(775)784-4583 fax www.cabnr.unr.edu/nres

MEMORANDUM

DATE: 5/20/2013

TO: Nevada Department of Wildlife Heritage Trust Committee

FROM: Cody Schroeder, Graduate Student

University of Nevada

SUBJECT: Extension of Heritage Grant #13-20

We would like to request an extension for our Wildlife Heritage Grant #13-20 titled “Investigation of trace mineral levels and forage quality of Nevada mule deer”, in the amount of $20,000 dollars. The reason we are requesting an extension is because the funds were not made available for us to spend until March 13, 2013 by the University of Nevada’s Office of Sponsored Projects. This prevented us from having sufficient time for spending the available funds on equipment and sub-agreements with the laboratory that will be conducting the forage quality analysis. We should be able to spend the funds and complete this project by December 2013. Please find the attached supporting documents showing our time constraints and sub-agreement with the fecal nitrogen analyses laboratory.

Best Regards,

Cody A Schroeder Cody Schroeder

University of Nevada Reno

From: Perry Barboza [mailto:psbarboza@alaska.edu] Sent: Wednesday, May 15, 2013 4:07 PM To: Schroeder, Cody Subject: Re: Fecal analysis #13-20 Cody: I think your best chance of assessing diet quality from your fecal samples is the analysis of N and fiber. Microhisitology or DNA sequencing will identify plant species or groups that will then be related to the composition, quality and biomass of the original items. However, I don't think you will necessarily need the details of a species identification to answer your question about differences between migratory and sedentary populations and between males and females. We should be able to run the samples this summer but I don't think we can finish all administration of the funds and the work before 1 July. Can you obtain a no-cost extension for your funds beyond 1 July? I will need to prepare a scope of work (a very brief proposal) with a budget for my administration at UAF to submit to UNR. If UNR accepts that proposal, UAF will allow me to spend funds and then request reimbursement from UNR when the work is completed. Are the samples dried or frozen? What is the average amount of material available from each sample (g wet or g dry)? Best Regards, Perry Barboza

Rev. 02/2012

13-Mar-13

TO:Principal Investigator(s): Dept Code

153 52 AG-EXP STATION G&C NATURAL RESOURCE & ENV SCI

153 52 AG-EXP STATION G&C NATURAL RESOURCE & ENV SCI

FROM:

Acct Number Amount

ACCOUNT NUMBER: L

OSPA:

Sponsor Award Number:

Sponsor Name: 632

Project Title:

AWARD: Sponsor Award:

Modification Amount:

Total Sponsor Award:

Memo Amount:

Memo Modification Amount:

Total Memo Amount: -

Total Amount

COST SHARE: Cost Sharing:

Modification Cost Sharing:

Total Cost Sharing:

TOTAL PROJECT COSTS:

PROJECT DATES:

START: 07/01/12 END: L = Lead, M = Memo, P = Program Income

Total Awarded Amt 20,000.00$

Comments:

Allocable and Allowable Costs Hiring of Personnel Fly America Act Provisions (Foreign Travel)

Human and Animal Subjects Committed and Actual Effort

Environmental Health & Safety Performance of Sub Awardees and Contractors

Conflict of Interest Award Reporting

It is the responsibility of the Principal Investigator to comply with all terms and conditions of this award as specified or

referenced by the award document. This may include, but is not limited to the following compliance topics:

Sponsored Projects/MS 325

Ph: 784-3561 Fax: 784-6680

Lana Caddel

caddel@unr.edu

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NV-DOW

TRACE MINERAL LEVELS/MULE DEER

06/30/13

1321 153 521X

1209086

13-20

20,000.00

-

Summary of Multiple Accounts

UNIVERSITY OF NEVADA, RENO

Department

STEWART, K.M.

NOTIFICATION OF SPONSORED AWARD

SCHROEDER,C.A.

DATE:

College

Investigators are also responsible to ensure that the work performed is consistent with the Scope of Work as approved. All

changes to the Scope of Work must be reported to sponsor by the Office of Sponsored Projects. Contact your pre award

analyst for assistance.

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20,000.00$

20,000.00$

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20,000.00$

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Original Modification Memo Memo-N/C Ext Off_Memo Stop Order