Henry J. Klassen, M.D., Ph.D. Gavin Herbert Eye Institute

University of California, Irvine Dept. of Ophthalmology & Stem Cell Research Center

Curriculum Vitae: January 8, 2012

Current Positions: Associate Professor and Director, Stem Cell & Retinal Regeneration Program, Department of

Ophthalmology, University of California, Irvine, 2011-present Adjunct Associate Scientist, the Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, 2004-present Visiting Professor, He College of Ophthalmology and Visual Science, Shenyang, China, 2008-

present Prior Positions Assistant Professor and Director, Stem Cell & Retinal Regeneration Program, Department of

Ophthalmology, University of California, Irvine, 2006-2011 Visiting Professor, Peking University Third Hospital, Beijing, 2007-2009 Senior Clinician-Scientist, Singapore Eye Research Institute, 2006 Visiting Associate Researcher, Stem Cell and Regeneration Program, the Burnham Institute,

2004-2009 Faculty, Human Embryonic Stem Cell Culture Training Course, Children’s Hospital of Orange County/Burnham Institute/National Institutes of Health, 2004-2009 Visiting Associate Researcher, Department of Ophthalmology, University of California, Irvine, 2003-2006 Director, Stem Cell Research, Children’s Hospital of Orange County, 1998-2006

Education and Training B.A., Neurobiology, University of California, Berkeley, 1979 M.S., Psychology, University of California, Santa Cruz, 1983 Ph.D., Neurobiology, Anatomy and Cell Science, University of Pittsburgh, 1989 M.D., University of Pittsburgh, 1991 Internship, Cambridge Hospital, Cambridge, MA, 1991-1992 Residency in Ophthalmology, Yale University, 1992-1995 Visiting Ophthalmologist, Princess Margaret Hospital, Nassau, Bahamas, 1994 Fellowship, Medical Retina and Retinal Research, Moorfields Eye Hospital and Institute of Ophthalmology, London, United Kingdom, 1995-1997 Clinical and Research Fellowships First Year Graduate Student Fellowship, University of California, 1980-1981 Mellon Foundation Fellowship, 1986-1991 Student Research Program Fellowship, University of Pittsburgh, 1986 Charles E. Culpeper Foundation Fellowship, 1987-1988 Clinical Fellow in Medicine, Harvard Medical School, 1991-1992 Foundation Fighting Blindness, Career Development Award, 1995 NATO Student Fellowship, June 1997 Awards

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B.A. degree with Distinction in General Scholarship, 1979 Pittsburgh Neuroscience Society Annual Award for Excellence in Basic Science Research, 1987 Citation for Excellence in Research, University of Pittsburgh School of Medicine, 1989 Outstanding Presentation of the 1989 Eastern Student Research Forum Certificate of Recognition for Excellence in Medical Student Research, AMA, 1989 Yale Alumni in Ophthalmology Resident Research Prize, 1994 Career Development Award, Foundation Fighting Blindness, 1997 Stewardship Stars Award, University of California, Irvine, 2011 Dean's Junior Physician/Scientist Award, Research Associates, UCI School of Medicine, 2011 Professional Societies American Association for the Advancement of Science Association for Research in Vision and Ophthalmology International Society for Cellular Therapy International Society for Eye Research International Society for Stem Cell Research Sigma Xi Society for Clinical and Translational Science Consultancies: University of Southern California, Metaphors for Connectivity, 2002-2003 Research Corporation Technologies, Tucson, 2002-2003 Schepens Eye Research Institute, Boston, 2004-2011 Human Embryonic Stem Cell Culture Training Course, 2004-2009 ReNeuron, Ltd., Guildford, Surrey, UK, 2006-2011 Foundation Fighting Blindness, Stem Cell Consortium, 2006-present AVOS Life Sciences, ad hoc, 2009 French National Research Agency (ANR), ad hoc, 2009 Stem Cells, Inc., Palo Alto, ad hoc, 2009 Davis Research, The Aim Forum, Long Beach, ad hoc, 2011 GlaxoSmithKline, ad hoc, 2011 Conference Organizational Committees Pink Tank I: Stem Cell Transplantation in the Pig, Boston, 2002 XVI International Congress of Eye Research, Retinal Stem Cell Symposium Co-Chair, Sydney,

Australia, 2004 Pink Tank II: Stem Cell Transplantation in the Pig, Arild, Sweden, 2006 Asia ARVO, Stem Cell Biology Symposium Co-Chair, 2007 Pacific Ocular Regenerate Biology Conference XII, Retina Stem Cell Session Chair, 2007 Pink Tank III: Modeling AMD Repair in the Pig Retina, Lead Organizer, Univ. of Calif. Irvine

Medical Center, 2007 XVIII International Congress of Eye Research, Retinal Stem Cell Symposium Co-Chair, Beijing,

China, 2008 Pink Tank IV: Modeling AMD Repair in the Pig Retina, Co-Organizer, Rockport, MA, 2008 ARVO Annual Meeting, Moderator, Poster Session 353, Stem Cells: Translational Research, Ft.

Lauderdale, 2010 XIX Biennial Meeting of the International Society for Eye Research, Session Chair, Drug

Delivery for Treatment of Retinal Degeneration, Montreal, 2010

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Invited Presentations at Professional Meetings American Medical Association, 1st Annual Symposium for Scientific Advancement and

Excellence in Medical Student Research, Chicago, 1989 6th Annual Vision Research Conference, ARVO Annual Meeting, Ft. Lauderdale, 2002 XV International Congress of Eye Research, Geneva, Switzerland, 2002 XVI International Congress of Eye Research, Sydney, Australia, 2004 Vision Down Under, ICER Satellite Meeting, Fraser Island, Australia, 2004 All-Myanmar Ophthalmology Symposium, Rangoon, Burma, 2005 Nanotechnology Applied to Ophthalmology, ARVO Special Topic Session, Ft. Lauderdale, 2005 Asia ARVO, Stem Cell Biology Symposium, Singapore, 2007 International Molecular Medicine Tri-Conference, Stem Cell Congress, San Francisco, 2008 XXXI World Ophthalmology Congress; Immunol., Mol. Biol. And Genetics; Hong Kong, 2008 XXXI World Ophthalmology Congress; New Technology: Bioeng./Nanotech.; Hong Kong, 2008 XVII International Congress of Eye Research, Beijing, China, 2008 XXXII SIMASP-UNIFESP, Sao Paulo, Brazil, 2009 ARVO Annual Meeting, Special Interest Group, Stem Cell Therapy: Challenges in Translation to

the Clinic, Ft. Lauderdale, 2009 24th Congress of the Asia-Pacific Academy of Ophthalmology, Nusa Dua, Indonesia, 2009 XXXII World Ophthalmology Congress, Berlin, 2010 XIX Biennial Meeting of the International Society for Eye Research, Montreal, 2010 4th Annual Retinal Innovations Symposium, Guest Speaker, Newport Beach, 2011 ARVO Annual Meeting, Special Interest Group, Cellular Therapy for the Dysfunctional RPE and

Retina: Delivery Methods including Cell Suspension and Cellular Sheets; Therapeutic Goal: Functional Replacement versus Neuroprotection, Ft. Lauderdale, 2011

Visual Restoration: Regenerative Medicine in Ophthalmology, University of Pittsburgh, Pittsburgh, 2011

5th Annual Gavin Herbert Eye Institute Colloquium, Newport Beach, 2011 Hereditary Retinal Diseases-from Research to Treatment, Department of Ophthalmology, Oslo

University Hospital and Norwegian Retinitis Pigmentosa Association, Oslo, 2011 Invited Presentations: Educational/Governmental Institutions University of Pittsburgh, Center for Neuroscience, 1987 University of Pittsburgh, School of Medicine, 1989 University of Wisconsin, Department of Ophthalmology, 1996 University of California, Los Angeles, Department of Pathology, 2000 Johns Hopkins University, Wilmer Eye Institute, Baltimore, 2000 University of California, Irvine, Tissue Engineering Group, 2001 Research Corporation Technologies, Tucson, 2001 University of Southern California, Design Fundamentals, 2001 University of California, Irvine, Visual Culture, 2002 University of Miami, Bascom Palmer Eye Institute, 2002 Children's Hospital of Los Angeles, Department of Ophthalmology, 2002 Pink Tank: Stem Cell Transplantation in the Pig, Boston, 2002 University of California, San Diego, Department of Bioengineering, 2002 Neural Stem Cell Think Tank, University of California, Irvine, 2003 Institute of Ophthalmology, London, England, 2003 University of California, Irvine, Center for Molec. and Mitochondrial Med. and Genetics, 2003 Harbor-UCLA Medical Center, Research & Education Inst., Los Angeles, 2003 University of Missouri, Columbia, Division of Biological Sciences, 2004

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University of Missouri, Columbia, Department of Ophthalmology, 2004 Burnham Institute, Human Embryonic Stem Cell Course, 2004 Children's Hospital of Orange County, Grand Rounds, 2004 University of Louisville, Kentucky, Department of Ophthalmology, 2004 The Glaucoma Foundation, Think Tank, New York City, 2004 Harbor-UCLA Medical Center, Research & Education Inst., Los Angeles, 2004 Peking University Eye Center, Beijing, China, 2004 Singapore Eye Research Institute, Singapore General Hospital, Singapore, 2005 Children's Hospital of Orange County, Human Embryonic Stem Cell Course, 2005 University of California, Irvine, Department of Ophthalmology, 2005 Iowa State University, Ames, Iowa, 2005 Discovery Eye Foundation, Los Angeles, 2005 Singapore National Eye Centre, Singapore, 2006 Retinitis Pigmentosa Society Singapore, 2006 Pink Tank II: Stem Cell Transplantation in the Pig, Arild, Sweden, 2006 ReNeuron, Guildford, UK, 2006 Peking University Eye Center, Beijing, China, 2006 Discovery Eye Foundation, Los Angeles, 2006 Foundation Fighting Blindness, Stem Cells for Inherited Retinal Diseases, Washington DC, 2006 Grand Rounds, University of California, Irvine, Department of Ophthalmology, 2007 Stem Cell Research Center, Stem Cells: Patient, Physician, Scientist, U.C. Irvine, 2007 Children's Hospital of Orange County, Human Embryonic Stem Cell Course, 2007 Peking University, Third Hospital, Beijing, China, 2007 City of Hope, Beckman Research Institute, Division of Neurosciences, 2007 Pink Tank III: Modeling AMD Repair in the Pig Retina, Univ. of Calif. Irvine Med. Center, 2007 Discovery Eye Foundation, Los Angeles, 2007 Southern California College of Optometry, Fullerton, CA, 2008 Children's Hospital of Orange County, Human Embryonic Stem Cell Course, 2008 He College of Ophthalmology and Visual Sciences, Shenyang, China, 2008 School of Medicine, Gyeongsang National University, Jinju, South Korea, 2008 Graduate Program, Gyeongsang National University, Jinju, South Korea, 2008 Pink Tank IV: Modeling AMD Repair in the Pig Retina, Rockport, MA, 2008 California State University Fullerton, Stem Cell Biology, Fullerton, CA, 2008 Children's Hospital of Orange County, Human Embryonic Stem Cell Course, 2009 Stem Cell Research Center, Journal Club, U.C. Irvine, 2009 Nippon Medical School, Department of Ophthalmology, 2009 Los Angeles Vision Symposium, Skirball Cultural Center, Los Angeles, 2009 California State University Fullerton, Stem Cell Biology, Fullerton, CA, 2009 Discovery Eye Foundation, Los Angeles, 2009 The National Academy of Sciences, Distinctive Voices @ The Beckman Center, Irvine, 2010 Discovery Eye Foundation, Los Angeles, 2010 University of California, Riverside, Department of Cell Biology and Neuroscience, 2010 Complutense University of Madrid, Ramon Castroviejo Institute of Ophthalmological

Investigation, 2010 Orange County Vision Symposium, U.C. Irvine, 2010 University of California, Irvine, Stem Cell Research Center, Stem Cell Techniques Course, 2010 University of California, Irvine, Stem Cell Research Center, Graham Group Reception, 2011 Foundation Fighting Blindness, Speaker Series, Braille Institute, Anaheim, 2011

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Invited Participation at International Meetings Beckman Initiative for Macular Research, Irvine, CA, 2009 4th Asia Bio-Business Partnering, Shenyang, China, 2009 Beckman Initiative for Macular Research, Irvine, CA, 2010 CIRM-Germany Stem Cell Workshop, San Francisco, 2010 (deferred) Beckman Initiative for Macular Research, Irvine, CA, 2011 Beckman Initiative for Macular Research, Irvine, CA, 2012 (pending)

Research Funding Previous Grants Edmond & Marianne Blaauw Grant for Research in Ophthalmology, Sigma Xi, 1988 Chairman's Grant for Research, Yale Eye Center, 1992 Postdoctoral Research Fellowship (NRSA), National Eye Institute, 1995-1997 CHOC Foundation and Padrinos, Stem Cell Research, 1999-2001, PI CHOC Padrinos, Immunogenicity of retino-tropic stem cells, 2001-2002, PI CHOC Foundation, Characterization and uses of CNS stem cells, 2002-2005, PI CHOC Foundation, Characterization and transplantation of human CNS stem cells from donated post-natal tissue, 2002-2005, Co-PI Stem Cell Research Foundation, Isolation of stem cells from the human neural retina, 2002-2003, renewed for 2003-2004, PI NIH/NINDS, Characterization of transgenic neural stem cells, R21 NS44060-01, 2002-2004, PI NIH/NINDS, Administrative supplement 3 R21 NS44060-01S1 for study of human embryonic stem cells, one time award, PI NIH/NINDS, Human embryonic stem cell culture training course, T15 HL074286-01, Co-PI Hoag Foundation, Development of a therapy for degeneration of the central retina, 2004-2005, PI NIST Advanced Technology Program, Novel technologies for adult stem cell therapeutics,

Proposal Number 2004-00-6034, 2005-2007, Collaborator Biomedical Research Council (A*Star BMRC Grant, Singapore), Isolation of multipotent

progenitor cells from the retina of the GFP-transgenic pig and transplantation to the pig retina in conjunction with biodegradable polymer scaffolds, 2006, PI

Foundation Fighting Blindness, Multipotent progenitor cells: isolation and transplantation to the retina, 2006-2007, PI

California Institute for Regenerative Medicine. Stem cells for neuroprotection of photoreceptors in retinitis pigmentosa, CIRM Disease Team Planning Award, 2008-2009, PI

Present Grants Lincy Foundation/Discovery Eye Foundation. Functional reconstruction of the outer retina,

2007-2011, Group Leader and Co-PI Andrei Olenicoff Memorial Foundation, unrestricted funds for research on RP, 2008-2010, PI Polly and Michael Smith Foundation, unrestricted funds for research on AMD, 2010-2011, PI Allergan. Development of a novel retinal stem cell-based therapy for retinitis pigmentosa. 2010,

Co-PI. Beckman Initiative for Macular Research, GDNF-microspheres for treatment of atrophic AMD, 2010, PI California Institute for Regenerative Medicine. CIRM Early Translational II Research Award

(RFA 10-01) Application TR2-01794: Human retinal progenitor cells as candidate therapy for retinitis pigmentosa, PI, Total Funds Requested (3 Years): $3,855,277. Scientific Review Final Score 93. Status: start date May 1, 2011.

Josephine Herbert Gleis Foundation, 2011, PI, $5,000

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NIH Study Sections National Heart, Lung, and Blood Institute, ad hoc reviewer, 2002 Neurogenesis and Cell Fate, ad hoc reviewer, 2005 hESC Challenge Grants, Subject Matter Expert, Special Expert Stage 1 reviewer, 2009

Other External Grant Reviews LA BioMed at Harbor-UCLA Medical Center, 2006 King/Drew Medical Center, 2007 Fight for Sight, UK, 2007 The M. J. Murdock Charitable Trust, 2008 Medical Research Council, UK, 2008 Agence Nationale de la Recherche, Programme Blanc, France, 2009 Scott & White Healthcare, Research Grants Program, 2009 Medical Research Council, UK, 2010 Fight for Sight, UK, 2010 CIMIT, Center for Integration of Medicine & Innovative Technology, Boston, 2010 Agence Nationale de la Recherche, Laboratories for Excellence, France, 2011 (invited, no need) CIMIT, Center for Integration of Medicine & Innovative Technology, Boston, 2011 Guide Dogs for the Blind Association, Hillfields, Reading, Berkshire, UK, 2011 Editorial Service Investigative Ophthalmology and Visual Science: ad hoc reviewer, since 1999 Experimental Eye Research: ad hoc reviewer, since 2001 Ophthalmic Research: ad hoc reviewer, since 2002 Human Gene Therapy: ad hoc reviewer, since 2003 Stem Cells: ad hoc reviewer, since 2003 Leonardo: expert opinion, 2003 Experimental Neurology: ad hoc reviewer, since 2004 Nature Biotechnology: ad hoc reviewer, since 2005 Developmental Brain Research: ad hoc reviewer, since 2005 Journal of Neuroscience: expert opinion, 2005 Cancer Cell International: ad hoc reviewer, since 2005 Acta Ophthalmologica Scandinavica: ad hoc reviewer, since 2006 Cell Death and Differentiation: ad hoc reviewer, since 2006 Journal of Neural Engineering: ad hoc reviewer, since 2006 European Journal of Neuroscience: ad hoc reviewer, since 2006 Journal of Neuroscience Methods: ad hoc reviewer, since 2007 Cell Research: ad hoc reviewer, since 2007 Stem Cells: ad hoc reviewer, since 2007 Neuroscience: ad hoc reviewer, since 2007 Molecular and Cellular Neuroscience: ad hoc reviewer, since 2008 Journal of Biomedical Materials Research: Part A: ad hoc reviewer, since 2008 Ophthalmic Research: ad hoc reviewer, since 2008 Current Stem Cell Research & Therapy: ad hoc reviewer, since 2008 Nature Protocols: ad hoc reviewer, since 2008 Clinical and Experimental Ophthalmology: ad hoc reviewer, since 2009 Stem Cells and Development: ad hoc reviewer, since 2009 Tissue Engineering: ad hoc reviewer, since 2009

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Journal of Neuroscience Research: ad hoc reviewer, since 2009 Regenerative Medicine: ad hoc reviewer, since 2009 Cell Proliferation: ad hoc reviewer, since 2009 Journal of Ocular Biology Diseases and Informatics: ad hoc reviewer, since 2009 Expert Opinion On Biological Therapy: ad hoc reviewer, since 2010 PLoS ONE: ad hoc reviewer, since 2010 Stem Cell Reviews and Reports, ad hoc reviewer, since 2010 Journal of Neuroscience: ad hoc reviewer, since 2010 Gene Therapy and Regulation: ad hoc reviewer, since 2010 Journal of Tissue Engineering and Regenerative Medicine: ad hoc reviewer, since 2011 ACS Nano: ad hoc reviewer, since 2011 Stem Cells International, ad hoc reviewer, since 2011 Neurotherapeutics, ad hoc reviewer, since 2011 Science Translational Medicine, ad hoc reviewer, since 2011 Lead Guest Editor, Special Issue: Stem Cells in Large Animal Models of Retinal and Neurological Disease, Stem Cells International, due 2012 (in progress)

External Faculty Examiner or Evaluator Christina Flaxel, MD, promotion to Clinical Associate Professor, Doheny Eye Institute, 2002 Anita Wojciechowski, doctoral dissertation, Lund University, 2004 Yau Wen Yee, Thesis: Bachelor of Medical Science, University of Melbourne, 2004 Mark Kirk, PhD, promotion to Professor, University of Missouri, 2004 Hui Shao, MD, promotion to Associate Professor, University of Louisville, 2005 Leonard Hetherington, doctoral dissertation, University of Sheffield, 2005 Sophia Liao, MS, predoctoral CIRM scholarship, University of California, Irvine, 2007 Sara Dawen Qi, Thesis: Bachelor of Medical Science, University of Melbourne, 2010 University Service, U.C. Irvine Faculty interviewer, student applicants to School of Medicine, 2010-present Executive Committee, Department of Ophthalmology, 2010-present

Intellectual Property Klassen, H., Whiteley, S., Young, M. Low dose IL-1β-induced photoreceptor rescue without retinal dysplasia. International Patent Application PCT/US00/12565. Abandoned. Young, M., Gage, F., Ray, J.,Whiteley, S., Klassen, H. Integration of transplanted neural progenitor cells into neural tissue of immature and mature recipients. International Patent Application PCT/US00/03534. Abandoned. Klassen, H., Schwartz, M., Young, M. Isolation of neural stem cells using gangliosides and other surface markers. U.S. Patent No. 7,419,825. Young, M., Klassen, H., Shatos, M., Mizumoto, K. Isolation and transplantation of retinal stem cells. U.S. Patent No. 7,514, 259. European patent No. 01 907 195.0 (1 261 357).

Young, M. and Klassen, H. Composite graft for treatment of retinal diseases. U.S. Patent Application No. 60/343,402. Abandoned.

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Desai, T., Tao, S., Young, M., Klassen, H. Topographically engineered structures and methods for using the same in regenerative medicine applications. International Application Number: PCT/US2008/056436. Tao, S, Klassen, H., Young, M. Ocular scaffolds and methods for subretinal repair of Bruch’s membrane. International Application Number: PCT/US2009/0306772. Klassen, H., Yang, J. Composition and methods for treating retinal diseases. Provisional application Number: 61487419. Refereed Journal Articles 1. Bridgeman, B. and Klassen, H. (1983) On the origin of stroboscopic induced motion. Percep. Psychophys. 34:149-154. 2. Klassen, H. and Lund, R. (1987) Retinal transplants can drive a pupillary reflex in host rat brains. Proc. Natl. Acad. Sci. USA 84: 6958-6960. 3. Klassen, H. and Lund, R. (1988) Anatomical and behavioral correlates of a xenograft-mediated pupillary reflex. Exp. Neurol. 102:102-108.

4. Klassen, H. and Lund, R. (1990) Retinal graft-mediated pupillary responses in rats: restoration of a reflex function in the mature mammalian brain. J. Neurosci. 10:578-587. 5. Klassen, H. and Lund, R. (1990) Parameters of retinal graft-mediated responses are related to underlying target innervation. Brain Res. 533:181-191.

6. Huncharek, M., Klassen, H. and Klassen, M. (1994) Splenic artery aneurysm and upper gastrointestinal bleeding in a nulliparous woman--a case history. Angiology 45:733-735. 7. Filatov, V., Tom, D., Alexandrakis, G., Skolik, S., Klassen, H. and Liggett, P. (1995) Branch retinal artery occlusion associated with directional coronary atherectomy after percutaneous transluminal coronary angioplasty. Am. J. Ophthalmol. 120:391-393. 8. Klassen, H., Klassen, M. and Huncharek, M. (1995) Group B streptococcal meningitis presenting as stroke in a nondebilitated man. Yale J. of Biol. and Med. 68:1-6. 9. Sauvé, Y., Klassen, H., Whiteley, S., and Lund, R. (1998) Visual field loss in RCS rats and the effect of RPE cell transplantation. Exp. Neurol. 152(2):243-250. 10. Schnell, L., Fearn, S., Klassen, H., Schwab, M., Perry, V.H. (1999) Acute inflammatory responses to mechanical lesions in the CNS: differences between brain and spinal cord. Eur. J. Neurosci. 11:3648-3658.

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11. Sauvé, Y., Klassen, H., Whiteley, S., and Lund, R. (1999) Age-dependent visual field loss in RCS rats and the relative benefits of subretinal over intravitreal RPE transplantation. Dig. J. Ophthalmol. 5(1). 12. Young, M., Ray, J., Whiteley, S., Klassen, H., Gage, F. (2000) Neuronal differentiation and morphological integration of hippocampal progenitor cells transplanted to the retina of immature and mature dystropic rats. Mol. Cell. Neurosci. 16 (3):1997-2005. 13. Shatos, M., Mizumoto, K., Mizumoto, H., Kurimoto, Y., Klassen, H., Young, M. (2001) Multipotent stem cells from the brain and retina of green mice. e-biomed: The Journal of Regenerative Medicine 2:13-15. 14. Mizumoto, H., Mizumoto, K., Whiteley, S., Shatos, S., Klassen, H., Young, M. (2001) Transplantation of human neural progenitor cells to the vitreous cavity of the Royal College of the Surgeons rat. Cell Transplant. 10:223-233. 15. Klassen, H., Whiteley, S., Young, M., Lund, R. (2001) Graft location affects functional rescue following RPE cell transplantation in the RCS rat. Exp. Neurol. 169:114-121. 16. Klassen, H., Schwartz, M., Bailey, A., Young, M. (2001) Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein epitopes. Neurosci. Lett. 312:180-182. 17. Whiteley, S., Klassen, H., Coffey, P., Young, M. (2001) Photoreceptor rescue after low dose intravitreal interleukin-1β injection in the RCS rat. Exp. Eye Res. 73:557-568. 18. Huncharek, M., Klassen, H., Kupelnick, B. (2001) Dietary beta-carotene intake and the risk of epithelial ovarian cancer: a meta-analysis of 3,782 subjects from five observational studies. In Vivo 15:339-343. 19. Huncharek, M., Kupelnick, B., Klassen, H. (2001) Paternal smoking during pregnancy and the risk of childhood brain tumors: Results of a meta-analysis. In Vivo 15:535-542. 20. Huncharek, M., Kupelnick, B., Klassen, H. (2002) Maternal smoking during pregnancy and the risk of childhood brain tumors: A meta-analysis of 6,566 subjects from twelve epidemiological studies. J. Neurooncol. 57(1):51-57. 21. Klassen, H., Imfeld, K., Ray, J., Young, Gage, F., M., Berman, M. (2003) The immunological properties of adult hippocampal progenitor cells. Vision Res. 43(8):947-956. 22. Mizumoto, H., Mizumoto, K., Shatos, M., Klassen, H., Young, M. (2003) Retinal transplantation of neural progenitor cells derived from the brain of GFP transgenic mice. Vision Res. 43(16):1699-1708. 23. Hori, J., Ng, T.-F., Shatos, M., Klassen, H., Streilein, J. W., Young, M. (2003) Neural stem cells lack immunogenicity and resist destruction as allografts. Stem Cells. 21(4):405-416. 24. Klassen, H., Imfeld, K., Kirov, I., Gage, F., Young, M., Berman, M. (2003) Expression of cytokines by multipotent neural progenitor cells. Cytokine. 22(3-4):101-106.

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25. Schwartz, P., Bryant, P., Fuja, T., Su, H., O'Dowd, D., Klassen, H. (2003) Isolation and characterization of neural progenitor cells from post-mortem human cortex. J. Neurosci. Res. 74:838-851. 26. Klassen, H., Ziaeian, B., Kirov, I., Young, M., Schwartz, P. (2004) Isolation of retinal progenitor cells from postmortem human tissue with comparison to autologous brain progenitors. J. Neurosci. Res. 77(3):334-343. 27. Klassen, H., Ng, T.-F., Kurimoto, Y., Kirov, I., Shatos, M., Coffey, P., Young, M. (2004) Multipotent progenitor cells express developmental markers, differentiate into retinal neurons, and preserve light-mediated behavior. Invest. Ophthalmol. Vis. Sci. 45:4167-4173. 28. Lavik, E., Klassen, H., Warfvinge, K., Langer, R., Young, M. (2005) Fabrication of degradable polymer scaffolds to direct the integration and differentiation of retinal progenitors. Biomaterials 26:3187-3196. 29. Zahir, T., Klassen, H., Young, M. (2005) Effects of ciliary neurotrophic factor on differentiation of late retinal progenitor cells. Stem Cells 23: 424-432. 30. Warfvinge, K., Kiilgaard, J., Lavik, E., Scherfig, E., Langer, R., Klassen, H., Young, M. (2005) Retinal progenitor cell xenografts to the pig retina: morphological integration and cytochemical differentiation. Arch. Ophthalmol. 123:1385-1393. 31. Schwartz, P, Nethercott, H., Kirov, I., Ziaeian, B., Young, M., Klassen, H. (2005) Expression of neurodevelopmental markers by cultured porcine neural precursor cells. Stem Cells 23:1286-1294. 32. Tomita, M., Lavik, E., Klassen, H., Zahir, T., Langer, R., and Young, M. (2005) Biodegradable polymer composite grafts promote the survival and differentiation of retinal progenitor cells. Stem Cells 23:1579-1588.

33. Warfvinge, K., Kiilgaard, J., Klassen, H., Zamiri, P., Scherfig, E., Streilein, J.W., Prause, J., Young, M. (2006) Retinal progenitor cell xenografts to the pig retina: immunological reactions. Cell Transplant. 15(7):603-612. 34. Yang, J., Klassen, H., Pries, M., Wang, W., Nissen, M. (2006) Aqueous humor enhances the proliferation of rat retinal precursor cells in culture and this effect is partially reproduced by ascorbic acid. Stem Cells 24:2766-2775. 35. Zahir, T., Tomita, M., Klassen, H., Young, M. (2006) Sorbitol promotes differentiation of late retinal progenitor cells into Müller glia in vitro. Molec. Vis. 12:1606-1614. 36. Klassen, H. (2007) Recruitment of endogenous neural progenitor cells by malignant neoplasms of the central nervous system. Curr. Stem Cell Res. & Ther. 2:113-119. 37. Zhang, Y., Klassen, H., Tucker, B., Perez, M-T, Young, M. (2007) CNS progenitor cells promote a permissive environment for neurite outgrowth via an MMP-dependent mechanism. J. Neurosci. 27:4499-4506.

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38. Klassen, H., Kiilgaard, J., Zahir, T., Ziaeian, B., Kirov, I., Scherfig, E., Warfvinge, K., Young, M. (2007) Progenitor cells from the porcine neural retina express photoreceptor markers after transplantation to the subretinal space of allorecipients. Stem Cells 25:1222-1230. 39. Tao, S., Young, C., Redenti, S., Zhang, Y., Klassen, H., Desai, T., Young, M. (2007) Survival, migration and differentiation of retinal progenitor cells transplanted on micro-machined poly(methyl methacrylate) scaffolds to the subretinal space. Lab Chip 7:695-701. 40. Klassen, H., Schwartz, P., Nethercott, H., Ziaeian, B., Young, M., Narfstrom, K. (2007) Neural precursors isolated from the developing cat brain show retinal integration following transplantation to the retina of the dystrophic cat. Vet. Ophthalmol. 10: 245-253. 41. Jiang, C., Moore, M., Zhang, X., Klassen, H., Langer, R., Young, M. (2007) Intravitreal injections of GDNF-loaded biodegradable microspheres are neuroprotective in a rat model of glaucoma. Molec. Vis. 13:1783-1792. 42. Neeley, W., Redenti, S., Klassen, H., Young, M., Langer, R. (2008) A microfabricated scaffold for retinal progenitor cell grafting. Biomaterials 29: 418-426. 43. Tucker, B., Klassen, H., Yang, L., Chen, DF, Young, M. (2008) Elevated MMP expression in the MRL mouse retina creates a permissive environment for retinal regeneration. Invest. Ophthalmol. Vis. Sci. 49:1686-1695. 44. Redenti, S., Tao, S., Yang , J., Gu, P., Klassen, H., Saigal, S., Desai, T., Young, M. (2008) Retinal tissue engineering using mouse retinal progenitor cells and a novel biodegradable, thin-film poly(e-caprolactone) nanowire scaffold. J. Oc. Biol. Dis. Informat. 1: 19-29. 45. Klassen, H., Warfvinge, K., Schwartz, P, Kiilgaard, J., Shamie. N., Jiang, C., Samuel, M., Scherfig, E., Prather, R., Young, M. (2008) Isolation of progenitor cells from GFP-transgenic pigs and transplantation to the retina of allorecipients. Cloning Stem Cells 10:391-402. 46. Yang, J., Klassen, H., Pries, M., Wang, W., Nissen, M. (2009) Vitreous humor and albumin augment the proliferation of cultured retinal precursor cells. J. Neurosci. Res. 87:495-502. 47. Redenti, S., Neeley, W., Rampani, S., Saigal, S., Yang, J., Klassen, H., Cepko, C., Langer, R., Young, M. (2009) Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation. Biomaterials 30:3405-3414. 48. Voss Kyhn, M., Warfvinge, K., Scherfig, E., Kiilgaard, J., Prause, J., Klassen, H., Young, M., la Cour, M. (2009) Acute retinal ischemia caused by controlled low ocular perfusion pressure in a porcine model. Electrophysiological and histological characterisation. Exp. Eye Res. 88:1100-1106.

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49. Aftab, U., Jiang, C., Tucker, B., Kim, J.-Y., Klassen, H., Miljan, E., Sinden, J., Young, M. (2009) Growth kinetics and transplantation of human retinal progenitor cells. Exp. Eye Res. 89:301-310. 50. Schmitt, S., Aftab, U., Jiang, C., Redenti, S., Klassen, H., Miljan, E., Sinden, J., Young, M. (2009) Molecular characterization of human retinal progenitor cells. Invest. Ophthalmol. Vis. Sci. 50:5901-5908. 51. Gu, P., Yang, J., Young, M., Klassen, H. (2009) Sequential changes in the gene expression profile of murine retinal progenitor cells during the induction of differentiation. Molec. Vis. 15:2111-2122. 52. Kyhn, M.V., Klassen, H., Johansson, U., Warfvinge, K., Lavik, E., Kiilgaard, J., Prause, J., Scherfig, E., Young, M., la Cour, M. (2009) Delayed administration of glial cell line-derived neurotrophic factor (GDNF) protects retinal ganglion cells in a pig model of acute retinal ischemia. Exp. Eye Res. 89:1012-1020. 53. Tucker, B., Redenti, S., Jiang, C., Swift, J., Klassen. H., Smith, M., Wnek, G., Young, M. (2010) The use of progenitor cell/biodegradable MMP2-PLGA polymer constructs to enhance cellular integration and retinal repopulation. Biomaterials 31:9-19. 54. Steedman, M., Tao, S., Klassen, H., Desai, T. (2010) Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues. Biomed. Microdevices 12:363-369. 55. Jiang, C., Klassen, H., Zhang, X., Young, M. (2010) Laser injury promotes migration and integration of retinal progenitor cells into host retina. Molec. Vis. 16:983-990.

56. Sodha, S., Wall, K., Redenti, S., Klassen, H., Young, M., Tao, S. (2010) Microfabrication of a three-dimensional polycaprolactone thin film scaffold for retinal progenitor cell encapsulation. J. Biomater. Sci. Polym Ed. June 21. Epub ahead of print. 57. Cho, S., Lee, Y., Lee, J., Yang, J., Klassen, H., Kong, I. (2010) Conformation of germ-line transmission in the red fluorescence protein (RFP) transgenic cloned male cat. Cell. Reprogram. 12:739-747. 58. Ejstrup, R., Kiilgaard, J., Tucker, B., Klassen, H., Young, M. La Cour, M. (2010) Pharmacokinetics of intravitreal glial cell line-derived neurotrophic factor: experimental studies in pigs. Exp. Eye Res. 91:890-895. 59. You, X.J., Gu, P., Wang, J., Song, T., Yang, J., Liew, C.G., Klassen, H. (2011) Efficient transduction of feline neural progenitor cells for delivery of glial cell line-derived neurotrophic factor using a feline immunodeficiency virus-based lentiviral construct. J. Ophthalmol. pii: 378965, Epub September 20, 2010. 60. Wang, J., Yang, J., Gu, P., Klassen, H. (2010) Effects of glial cell line-derived neurotrophic factor on cultured murine retinal progenitor cells. Molec. Vis. 16:2850-2866.

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61. Tucker, B., Park, I.H., Klassen, H., Redenti, S., Jiang, C., Qi, S., Daley, G., Young, M. (2011) Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in retinal degenerative mice. PLoS ONE 6(4): e18992. doi:10.1371/journal.pone.0018992. 62. Warfvinge, K., Schwartz, P., Kiilgaard, J., la Cour, M., Young, M., Scherfig, E., Klassen, H. (2011) Xenotransplantation of human neural progenitor cells to the subretinal space of non-immunosuppressed pigs. J. Transplant. 2011:948740, doi:10.1155/2011/948740. 63. Christiansen, A., Kiilgaard, J., Smith, M., Ejstrup, R., Wnek, G., Prause, J., Young, M., Klassen, H., Kaplan, H., la Cour, M., The influence of brightness on functional assessment by mfERG; a study on scaffolds used in retinal cell transplantation in pigs. Stem Cells Intl. In press. 64. Torrez, L.B., Perez, Y., Yang, J., zur Nieden, N., Klassen, H., Liew, C. Derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells. Stem Cells Intl. In press.

65. Christiansen, A., Tao, S., Smith, M., Wnek, G., Prause, J., Young, M., Klassen, H., Kaplan, H., la Cour, M., Kiilgaard, J. Subretinal implantation of electrospun, short nanowire and smooth poly (ε-caprolactone) (PCL) scaffolds to the subretinal space of porcine eyes. Stem Cells Intl. In press. 66. You, J., Yang, J., Gu, P., Liew, C., Klassen, H. Feline neural progenitor cells II: Use of a novel plasmid vector and hybrid promoter to drive expression of a glial cell line-derived neurotrophic factor transgene in feline neural progenitor cells. Stem Cells Intl. In press. Articles in Progress

Voss Kyhn, M., Kiilgaard, J., Garcia Lopez, A., Klassen, H., Young, M., Scherfig, E., la Cour, M. The induced multifocal electroretinogram (mfERG) in the pig. In revision.

Klassen, H., Kiilgaard, J.F., Warfvinge, K., Prather, R., Petters, R., la Cour, M., Young, M. Cultured retinal progenitor cells exhibit an advanced level of photoreceptor differentiation following transplantation to the retinal dystrophic pig. In revision. Yang, J., Gu, P., Menges, S., Klassen, H. Quantitative changes in gene transcription during induction of differentiation in porcine neural progenitor cells: implications for graft tolerance. In revision. Jiang, C., Klassen, H., Zhang, X., Young, M. Repopulation of outer retina following retinal laser injury by retinal progenitor cell transplantation. In review. Yang, J., Wang, J., Gu, P., You, J., Klassen, H. Feline neural progenitor cells I: Long-term expansion under defined culture conditions. In review.

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Reviews

1. Klassen, H., Sakaguchi, D., Young, M. (2004) Stem cells and retinal repair. Prog. Retin. Eye Res. 23:149-181. 2. Klassen, H. (2006) Transplantation of cultured progenitor cells to the mammalian retina. Expert Opin. Biol. Ther. 6:443-451.

Book Chapters

1. Lund, R., Radel, J., Hankin, M., Klassen, H., Coffey, P. and Rawlins, J. (1989) Developmental and functional integration of retinal transplants with host rat brains. Brain Repair Symposium, Wenner Gren Center International Conference, Stockholm, Sweden. 2. Flaxel, C., Klassen, H. and Gregor, Z. (1997) Antiangiogenic agents in vitreoretinal disease. In Ophthalmology Clinics of North America: Advances in Ocular Pharmacology. 10(3):421-433. 3. Lund, R., Klassen, H. and Young, M. (2000) Retinal transplantation and the pupillary light reflex. In Nervous Control of the Eye, Burnstock, G. and Sillito, A., eds., Volume 13 in the series: The Autonomic Nervous System, Harwood Academic Publishers, Amsterdam. 13:211-234. 4. Klassen, H., Young, M., Ritch, R., Richards, J., Borras, T., Levin, L. (2007) Retinal stem cell research. Chapter 68 in Vitreoretinal Surgical Techniques, 2nd edition, Peyman, Meffert, and Conway, eds. Abingdon, Oxfordshire, UK: Taylor & Francis. 668-676.

5. Yau, J., Klassen, H., Zahir, T., and Young, M. (2007) Stem cells and retinal transplantation. Chapter 20 in Retinal Degenerations: Biology, Diagnostics, and Therapeutics, Tombran-Tink, J. and Barnstable, C. eds. Humana Press. 385-400. 6. Li, S., Kirov, I., Klassen, H., Schwartz, P. (2007) Characterization of human stem cells using reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR (rtqRT-PCR). Chapter 10 in Human Stem Cell Manual: A Laboratory Guide, Loring, J, Wesselschmidt, R., and Schwartz, P., eds., Elsevier. 129-150. 7. Ng, T.-F., Klassen, H., Hori, J., and Young, M. (2007) Retinal transplantation. In Immune Response and the Eye, 2nd edition. Niederkorn, J. and Kaplan, H., eds. A volume in the series Chemical Immunology and Allergy. Karger AG (Basel). 92:300-16. 8. Hori, J., Ng, T.-F., Shatos, M., Klassen, H., Streilein, J. W., Young, M. (2007) Neural stem cells lack immunogenicity and resist destruction as allografts. In Ocul. Immunol. Inflamm. 15(3):261-273. 9. Sakaguchi, D., Klassen, H., Young, M. (2007) Transplantation of neural progenitors to the developing retina. In Strategies for retinal tissue repair and regeneration in vertebrates: from fish to human. Chiba, C., ed., Research Signpost. 113-137.

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10. Klassen, H. (2008) Progenitor cell transplantation for retinal disease. In Stem Cell Research and Therapeutics, Shi, Y., and Clegg, D., ed.s. Springer. 25-44. 11. Tucker, B., Klassen, H., Young, M. (2010) Cellular repopulation of the retina. Chapter 77 in Ocular Disease: Mechanisms and Management. Levin, L., and Albert, D., eds. 607-611. 12. Tucker, B., Klassen, H., Young, M. (2010) Injury and repair: stem cells and transplantation. Chapter 221 in Encyclopedia of the Eye. Dart, D., ed. Elsevier. 421-427. 13. Redenti, S., Klassen, H., Young, M. (2011) Tissue Engineering of Organs: Eye/retina. In The Tissue Engineering Book: State of the Art, Visions, and Limitations. Pallua, ed. Springer. Part 2, 335-346. 14. Tao, S., Klassen, H. Tissue engineering in the retina. In Stem Cell Transplantation to the Retina: Development, Plasticity, Regeneration and Repair. Sakaguchi, D., Young, M., and Klassen H., eds. World Scientific. In press. 15. Klassen, H., translated from English to Portuguese by Abud, M. (2010) Perspectivas do potencial das células progenitoras e seu uso para o tratamento de doenças da retina (Perspective on the potential use of progenitor cells for treatment of retinal disease). Chapter 37 in Vitrectomia 20, 23 E 25G. Avila, M., Isaac, D., and Santos, R., eds. Cultura Medica. 387-394.

Invited Commentaries and Published Letters 1. Klassen, H. (1990) The discovery of the graft-mediated pupillary reflex. JAMA 263:760. 2. Huncharek, M., Klassen, H. (1993) Acyclovir in varicella pneumonia in healthy adults. Respiration 60:254-255. 3. Young, M., Klassen, H. (2006) Polymeric delivery of neuroprotective compounds to the vitreous cavity: a potential treatment for glaucoma. Arch. Soc. Esp. Oftalmol. 81:429-432. 4. Young, M., Tucker, B., Klassen, H. (2007) Stemming the tide of retinal degeneration. Retinal Physician. May issue, online article 100301. 5. Klassen, H. and Reubinoff, B. (2008) Stem cells in a new light. Nature Biotechnol. 26: 187-188.

Published Abstracts 1. Thind, K., Neiman, R. and Klassen, H. (1985) Contact liquid crystal thermography in carpal tunnel syndrome. Proceedings of the 1985 Meeting of the Archives of Physical Medicine and Rehabilitation.

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2. Klassen, H. and Lund, R. (1987) Retinal transplants mediate a pupillary reflex in host rats. Soc. for Neurosci. Abstr. 13:290. 3. Klassen, H. and Lund, R. (1988) Anatomical and behavioral correlates of xenograft-mediated pupillary reflex. Soc. for Neurosci. Abstr. 14:1276. 4. Klassen, H. and Lund, R. (1988) Retinal transplants can make functional connections with the mature brain. Soc. for Neurosci. Abstr. 14:1277. 5. Radel, J., Klassen, H. and Lund, R. (1988) Transplant and host retinal inputs interact to drive pupillary reflex. Soc. for Neurosci. Abstr. 14:1277. 6. Radel, J., Klassen, H. and Lund, R. (1989) Functional interactions of transplant and host retinal inputs: the pupillary reflex. IIIrd International Symposium on Neural Transplantation, Restorative Neurology and Neuroscience, Suppl. 7. Klassen, H. and Lund, R. (1990) The magnitude of a graft-mediated reflex correlates with the amount of graft-specific immunoreactivity in the appropriate target nucleus. The Second Annual Bristol-Myers Squibb Symposium on Neuroscience Research: Plasticity and Pathology in the Damaged Brain. 8. Young, M., Klassen, H. and Lund, R. (1991) Effects of lesions of the olivary pretectal nucleus on the direct and consensual pupillary reflexes. Soc. for Neurosci. Abstr. 17:558. 9. Klassen, H., Klassen, M. and Barnstable, C. (1993) Cotransplantation of embryonic whole eye and tectum to the anterior chamber of the rat. Invest. Ophthalmol. & Vis. Sci. 34 (4, suppl): 1097. 10. Klassen, H., Klassen, M., Chaudhry, N., Liggett, P. and Barnstable, C. (1993) Successful development of mammalian embryonic eye transplants within the anterior chamber. Soc. for Neurosci. Abstr. 19:1513. 11. Klassen, H., Klassen, M., Chaudhry, N. and Liggett, P. (1994) Transplantation of embryonic eyes to the rabbit anterior chamber. Invest. Ophthalmol. & Vis. Sci. 35 (4, suppl):1524. 12. Klassen, H., Chaudhry, N., Klassen, M. and Liggett, P. (1995) In vivo evaluation of vascular integrity in intraocular grafts using fluorescein angiography. Invest. Ophthalmol. & Vis. Sci. 36 (4, suppl):551. 13. Sauvé, Y., Klassen, H., Whiteley, S., Litchfield, T. and Lund, R. (1996) Functional preservation following RPE grafting: I. Retinotopic mapping in the superior colliculus. Invest. Ophthalmol. & Vis. Sci. 37 (3, suppl):93. 14. Whiteley, S., Klassen, H., Sauvé, Y., Litchfield, T. and Lund, R. (1996) Functional preservation following RPE grafting: II. Correlation of SC responses with pupillometry and anatomy. Invest. Ophthalmol. & Vis. Sci. 37 (3, suppl):93.

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15. Sauvé, Y., Klassen, H., Whiteley, S., Litchfield, T. and Lund, R. (1996) Retinotopic analysis of response parameters in the superior colliculus of RCS rats following retinal pigment epithelial cell transplantation. Soc. for Neurosci. Abstr. 22:1978. 16. Klassen, H., Sauvé, Y., Whiteley, S., and Lund, R. (1997) Pigment epithelial cell grafting in the dystrophic rat: Correlation of function and anatomy. Invest. Ophthalmol. & Vis. Sci. 38 (suppl):946. 17. Whiteley, S., Klassen, H., and Lund, R. (1998) Altitudinal position of subretinal RPE grafts affects pupillary performance in RCS rats. Invest. Ophthalmol. & Vis. Sci. 39 (4, suppl):440. 18. Klassen, H., Whiteley, S., and Lund, R. (1998) Reaction of non-neuronal cells to subretinal injury in the dystrophic rat. Invest. Ophthalmol. & Vis. Sci. 39 (4, suppl):441. 19. Young, M., Klassen, H., Whiteley, S., and Lund, R. (1998) Long-term assessment of photoreceptor rescue in the RCS rat following laser photocoagulation. Invest. Ophthalmol. & Vis. Sci. 39 (4, suppl):3839. 20. Klassen, H., Whiteley, S., and Young, M. (1999) Photoreceptor rescue in the RCS rat following intravitreal IL-1β injections without concomitant retinal dysplasia. Invest. Ophthalmol. & Vis. Sci. 40 (suppl):43. 21. Whiteley, S., Ray, J., Klassen, H., Young, M., and Gage, H. (1999) Survival and integration of neural progenitor cells transplanted into the dystrophic mouse retina. Invest. Ophthalmol. & Vis. Sci. 40 (suppl):113. 22. Young, M., Ray, J., Whiteley, S., Klassen, H., and Gage, H. (1999) Integration of transplanted neural progenitor cells into the retina of the dystrophic rat. Invest. Ophthalmol. & Vis. Sci. 40 (suppl):113. 23. Klassen, H., Imfeld, K., Whiteley, S., Ray, J., Young, Gage, F., Berman, M. (1999) Immunological characteristics of clonally-derived adult hippocampal progenitor cells. EMBO Workshop: Stem Cells, Growth Factors and Cancer. 24. Klassen, H., Imfeld, K., Young, M., Berman, M. (1999) Production of interleukin-1β by human neural progenitor cells. Soc. for Neurosci. Abstr. 25:1535. 25. Whiteley, S., Mizumoto, K., Mizumoto, H., Klassen, H., Young, M. (1999) Transplantation of human embryonic progenitor cells into an animal model of retinal degeneration. Soc. for Neurosci. Abstr. 25:213. 26. Young, M., Ray, J., Whiteley, S., Klassen, H., and Gage, F. (1999) Neuronal differentiation and morphological integration of hippocampal progenitor cells transplanted to the retina of immature and mature dystrophic rats. Soc. for Neurosci. Abstr. 25:2263. 27. Klassen, H., Imfeld, K., Young, M., Ray, J., Gage, F., Berman, M. (2000) Immunological characteristics of a progenitor cell line capable of retinal integration. Invest. Ophthalmol. & Vis. Sci. 41 (suppl):15.

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28. Shatos, M., Mizumoto, K., Mizumoto, H., Whiteley, S., Klassen, H., Young, M. (2000) Isolation and characterization of retinal progenitor cells from GFP transgenic mice. Invest. Ophthalmol. & Vis. Sci. 41 (suppl):747. 29. Klassen, H., Young, M. (2000) Adult hippocampal progenitor cells: immunological characteristics in vitro. ISDN 2000:63. 30. Young, M., Klassen, H. (2000) CNS stem cell transplants in rodent models of retinal degeneration. ISDN 2000:67. 31. Klassen, H., Imfeld, K., Young, M., Ray, J., Gage, F., Berman, M. (2000) Rat hippocampal progenitor cells do not elicit an immunological response in vitro. Soc. for Neurosci. Abstr. 26: 12. 32. Klassen, H., Young, M. (2001) Transplantation of stem cells to the retina. Twenty First Moorfields Alumni Meeting.

33. Klassen, H., Gage, F., Young, M. (2001) Retino-tropic neural stem cells up-regulate transplantation antigens in response to IFN-γ. Invest. Ophthalmol. & Vis. Sci. 42 (suppl):198. 34. Lauritzen, D., Kurimoto, Y., Gage, F., Klassen, H., Young, M. (2001) Retinal transplantation of neural progenitor cells in rats transgenic for mutant rhodopsin. Invest. Ophthalmol. & Vis. Sci. 42 (suppl):198. 35. Whiteley, S., Klassen, H., Coffey, P., Young, M. (2001) IL-1β ameliorates visual function deterioration and photoreceptor degeneration in the RCS rat. Invest. Ophthalmol. & Vis. Sci. 42 (suppl):631. 36. Schwartz, M., Bailey, A., Young, M., Klassen, H. (2001) Surface marker expression by neural progenitor cells. Soc. for Neurosci. Abstr. 27:1525. 37. Ng, TF, Hori, J, Klassen, H., Streilein, JW, Young, M. (2001) Comparison of the immunogenicity of neonatal brain and neural stem cells. Soc. for Neurosci. Abstr. 27:1565. 38. Klassen, H., Schwartz, M., Shatos, M., Young, M. (2002) Multipotent progenitor cells from the neural retina express identified surface markers and respond to cytokines. Invest. Ophthalmol. & Vis. Sci. 43 (suppl):157. 39. Klassen, H., Young, M. (2002) Expression of transplantation antigens by CNS stem cells. Sixth Annual Vision Research Conference, Retinal Cell Rescue. 40. Klassen, H., Young, M. (2002) Expression of MHC antigens by stem cells of the central nervous system. XV International Congress of Eye Research. 41. Warfvinge, K., Kiilgaard, J., Lavik, E., Klassen, H., Scherfig, E., Langer, R., Young, M. (2003) Survival, integration and differentiation of retinal progenitor cells from GFP transgenic mice transplanted subretinally to adult, normal pigs. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 483.

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42. Kiilgaard, J., Scherfig, E., Klassen, H., Warfvinge, K., Prause, J., Young, M. (2003) Transplantation of xenogeneic retinal stem cells to pig subretinal space. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 487. 43. Lavik, E., Klassen, H., Warfvinge, K., Scherfig, E., Kiilgaard, J., Langer, R., Young, M. (2003) Polymer scaffolds provide support and guidance for retinal stem cells in retinal degeneration models. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 508. 44. Zahir, T., Lavik, E., Klassen, H., Langer, R., Young, M. (2003) Differentiation of retinal progenitor cells into specific cell types. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1666.

45. Klassen, H., Schwartz, M., Zahir, T., Schwartz, P., Young, M. (2003) Human retinal progenitors can be cultured from cadaveric tissue and express GD2 ganglioside and other known surface markers. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1691. 46. Shatos, M., Klassen, H., Scherfig, E., Kiilgaard, J., Warfvinge, K., Prause, J., Young, M. (2003) Isolation, characterization, and expansion of porcine retinal progenitor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1694. 47. Klassen, H., Schwartz, P., Ziaeian, B., Kirov, I., Young, M. (2003) Human retinal progenitors can be cultured from postmortem tissue and express a range of known primitive and mature markers. Soc. for Neurosci. Abstr. 453.3 48. Schwartz, P., Ziaeian, B., Kirov, I., Fuja, T., Su, H., Bryant, P., O'Dowd, D., Klassen, H. (2003) Banking human neural stem cells from postmortem brain. Soc. for Neurosci. Abstr. 453.4 49. Warfvinge, K., Kiilgaard, J., Klassen, H., Scherfig, E., Shatos, M., Holmqvist, B., Young, M. (2003) Survival, integration and differentiation of retinal progenitor cells from fetal pigs after subretinal transplantation to adult normal pigs. Soc. for Neurosci. Abstr. 453.5 50. Ng, T.-F., Young, M., Mwidau, A., Klassen, H., Coffey, P. (2003) Photoreceptor rescue, differentiation and light perception In Rhodopsin knock-out mice with transplantation of retinal progenitor cells. Soc. for Neurosci. Abstr. 453.6 51. Lavik, E., Coffey, P., Klassen, H., Warfvinge, K., Kiilgaard, J., Scherfig, E., Langer, R., Young, M. (2003) Biodegradable polymer scaffolds promote survival and differentiation of retinal progenitor cells. Soc. for Neurosci. Abstr. 697.9 52. Klassen, H., Lavik, E., Warfvinge, Langer, R., Young, M. (2003) Polymer scaffolds and stem cells in retinal transplantation. 7th US-Japan Symposium on Drug Delivery Systems Abstr. 13

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53. Shamie, N., Almeda, T., Schwartz, P., Klassen, H., Kenney, M. (2004) Localization of P-63 to fluorescent GFP expressing cells in a corneal epithelial cell culture derived from cornea-scleral rim explants of GFP transgenic pig. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1440. 54. Zahir, T., Shatos, M., Young, M., Klassen, H. (2004) Ubiquitous expression of protein kinase C (PKC) alpha by retinal progenitor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5391. 55. Klassen, H., Warfvinge, K., Kiilgaard, J., Scherfig, E., Prause, J., Shatos, M., Samuel, M., Prather, R., Young, M. (2004) Transplantation of retinal progenitor cells from GFP-transgenic pigs to the injured retina of allogeneic recipients. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5400. 56. Shatos, M., Klassen, H., Schwartz, P., Doherty, J., Ziaeian, B., Kirov, I., Nethercott, H., Samuel, M., Prather, R., Young, M. (2004) Isolation of progenitor cells from the retina and brain of the GFP-transgenic pig. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5406. 57. Klassen, H., Young, M., Schwartz, P. (2004) Human retinal progenitor cells from postmortem tissue. XVI International Congress of Eye Research. 58. Klassen, H., Young, M. (2004) Marker expression by cultured CNS progenitor cells. Vision Down Under, Fraser Island, Australia.

59. Schwartz, P., Loring, J., Derzic, S., Nethercott, H., Ziaeian, B., Kirov, I., Klassen, H. (2004) Differences among neural progenitor cell cultures determined by gene expression analysis. Soc. for Neurosci. Abstr. 60. Klassen, H. (2005) Roles for micro- and nanotechnology in retinal ganglion cell rescue and replacement. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1087. 61. Klassen, H., Warfvinge, K., Schwartz, P., Young, M., Bragadóttir, R., Meyer, J., Nethercott, H., Kirk, M., Narfström, K. (2005) Isolation and characterization of feline neural progenitor cells and transplantation to the retina of the Abyssinian cat with hereditary rod cone degeneration. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3218. 62. Tomita, M., Lavik, E., Klassen, H., Zahir, T., Langer, R., Young, M. (2005) Enhanced survival and differentiation into photoreceptor in retinal progenitor cells transplantation using polymer composite graft. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3227. 63. Warfvinge, K., Schwartz, P., Kiilgaard, J., Nethercott, H., Scherfig, E., Young, M., Klassen, H. (2005) Transplantation of human neural progenitor cells to pig subretinal space. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3228.

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64. Young, M., Klassen, H., Warfvinge, K. (2005) Morphological characterization of fetal GFP transgenic pig retinal progenitor cells after subretinal transplantation to adult normal pigs. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3251. 65. Yang, J, Wang, W, Pries, M, Ropke, C, Klassen, H., Nissen, M (2006) The role of aqueous humor in the proliferation of retinal progenitor cells Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5739. 66. Zhang, Y., Klassen, H., Perez, M-T, Young, M. (2006) CNS progenitor cells promote a permissive environment for neurite outgrowth via an MMP-dependent mechanism. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5751. 67. Li, S., Loudon, W., Lavik, E., Young, M., Klassen, H. (2006) Monitoring marker gene expression of stem cells cultured in biodegradable polymer scaffolds. Gordon Conference. 68. Klassen, H. (2007) Culturing progenitor cells from the mammalian retina. Asia ARVO, presentation S13.2. 69. Yang, J., Klassen, H., Pries, M., Wang, W., Nissen, M. (2007) Vitreous humor enhances the proliferation of retinal precursor cells. Asia ARVO, abstract 260. 70. Voss Kyhn, M., Kiilgaard, J., Young, M., Klassen, H., Lavik, E., Scherfig, E., Warfvinge, K., la Cour, M. (2007) Neuroprotection of ischemic injury in pig retina, Part I: The injury model. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 566. 71. Eftekhari, S., Voss Kyhn, M., Lavik, E., Kiilgaard, J., Klassen, H., Scherfig, E., Young, M., Warfvinge, K. (2007) Neuroprotection of ischemic injury in pig retina, Part II: Ganglion cell rescue. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 567. 72. Lavik, E., Voss Kyhn, M., Warfvinge K., Kiilgaard, J., Scherfig, E., la Cour, M., Klassen, H., Young, M. (2007) Neuroprotection of ischemic injury in pig retina, Part III--Functional assessment. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 568. 73. Redenti, S., Tao, S., Young, C., Zhang, Y., Klassen, H., Desai, T., Young, M. (2007) Retinal progenitor cells delivered on micro-machined poly(methyl methacrylate) scaffolds survive, migrate and differentiate in host retina after sub-retinal transplantation. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4067. 74. Yang, J., Klassen, H., Pries, M., Wang, W., Nissen, M. (2007) Vitreous humor enhances the proliferation of retinal precursor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4075.

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75. Narfstrom, K., Warfvinge, K., Schwartz, P., Young, M., Tullis, G., Eftekhari, S., Hamm C., Hainsworth, D., Klassen, H. (2007) Integration of feline neural precursor cells after subretinal transplantation into Abyssinian cats affected with hereditary retinal degeneration. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4064. 76. Aftab, U., Jiang, C., Klassen, H., Miljan, E., Sinden, J., Young, M. (2007) Isolation, growth kinetics and differentiation of human retinal progenitor cells in culture. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4076. 77. Steedman, M., Tao S.L., Klassen, H., Desai, T. (2007) Biodegradable thin film scaffolds for retina. Biomedical Engineering Society, Annual Fall Meeting. 78. Tucker, B., Redenti, S., Klassen, H., Smith, M., Wnek, G., Young, M. (2007) Delivery of active matrix metalloproteinase-2 via a biodegradable polymer induces degradation of inhibitory ECM molecules and enhanced stem cell migration. Pacific Ocular Regenerative Biology Conference. 79. Redenti, S., Tao, S., Klassen, H., Desai, T., Young, M. (2007) Tissue engineering using mouse retinal progenitor cells and a novel biodegradable poly(ε-caprolactone) nanofiber scaffold. Pacific Ocular Regenerative Biology Conference. 80. Jiang, C., Klassen, H., Young, M. (2007) Reconstruction of outer retina following retinal laser injury by retinal progenitor cell transplantation. Pacific Ocular Regenerative Biology Conference. 81. Aftab, U., Jiang, C., Klassen, H., Miljan, E., Sinden, J., Young, M. (2007) Isolation, growth kinetics and differentiation of human retinal progenitor cells in culture. Pacific Ocular Regenerative Biology Conference. 82. Gu, P., Yang, J., Young, M., Klassen, H. (2008) Sequential changes in the gene expression profile of retinal progenitor cells during the induction of differentiation. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3560.

83. Yang, J., Gu, P., Tao, S., Desai, T., Klassen, H. (2008) Sequential quantitative profiling of porcine CNS progenitor cells on biodegradable nanowire scaffolds. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4777.

84. Jiang, C., Klassen, H., Zhang, X., Young, M. (2008) Repopulation of outer retina following retinal laser injury by retinal progenitor cell transplantation. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3556. 85. Tucker, B., Redenti, S., Klassen, H., Smith, M., Wnek, G., Young, M. (2008) Enhanced progenitor cell integration following transplantation of progenitor cell/MMP2-polymer

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constructs. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3567. 86. Schmitt, S., Aftab, U., Jiang, C., Tucker, B., Stevanato, L., Chander, S., Miljan, E., Sinden, J., Klassen, H., Young, M. (2008) Molecular characterization and transplantation of human retinal progenitor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3571. 87. Klassen, H. (2008) Transplantation of CNS progenitor cells to the mammalian retina. International Molecular Medicine Tri-Conference, Stem Cell Congress, San Francisco, 2008 88. Klassen, H. (2008) Stem Cell Transplants in Animal Models of Retinal Degeneration. World Congress of Ophthalmology, Program on New Technology: Bioengineering/Nano-technology. 89. Klassen, H. (2008) Cultured Retinal Progenitor Cells. World Congress of Ophthalmology, Program on Immunology, Molecular Biology and Genetics.

90. Gu, P., Yang, J., Young, M., Klassen, H. (2008) Quantitative changes in the gene expression profile of murine retinal progenitor cells during early differentiation. XVIII International Congress of Eye Research.

91. Yang, J., Gu, P., Tao, S., Desai, T., Klassen, H. (2008) Quantitative changes in the gene expression of porcine CNS progenitor cells cultured on nanowire scaffolds. XVIII International Congress of Eye Research. 92. Klassen, H. (2008) Cultured CNS progenitors in animal models of retinal disease. XVIII International Congress of Eye Research. 93. Yang, J., Gu, P., Klassen, H. (2009) CNTF induces rapid expression of neuronal and glial transcripts in cultured porcine retinal progenitor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1266. 94. Gu, P., Yang, J., Lee, Y.-S., Narfstrom, K., Kong, I.-K., Klassen, H. (2009) Isolation, proliferation, and characterization of retinal progenitor cells from domestic and RFP-transgenic cats. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 5137. 95. Wang, J.-M., Yang, J., Gu, P., Young, M., Klassen, H. (2009) The proliferation of murine retinal progenitor cells is fundamentally dependant on EGF yet relatively independent of GDNF. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1272. 96. Redenti, S., Neeley W., Rampani, S., Yang, J., Klassen, H., Langer, R.,Young, M. (2009) Engineering retinal progenitor cell and scrollable poly(Glycerol-Sebacate) composites for expansion and subretinal transplantation. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 2409.

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97. Ejstrup, R., Kiilgaard, J., Tucker, B., Klassen, H., Young, M., la Cour, M. (2009) Pharmacokinetics of intravitreal glial cell line-derived neurotrophic factor: experimental studies in pigs. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 6234. 98. Jiang, C., Aftab, U., Klassen, H., Zhang, X., Young, M. (2009) Human retinal progenitor cells integrate as photoreceptors after transplantation into the subretinal space of mice. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1289. 99. Tucker, B., Park, I.H., Klassen, H., Redenti, S., Jiang, C.H., Qi, S., Daley, G., Young, M. (2010) Transplantation of adult mouse iPS cell-derived photoreceptor precursors restores retinal structure and function in retinal degenerative mice. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 1233. 100. Yang, J., Gu, P., Klassen, H. (2010) Molecular characterization of human retinal progenitor cells during long term culture. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 2649. 101. Wang, J., Yang, J., Klassen, H. (2010) GDNF augments proliferation and reduces apoptosis of murine retinal progenitor cells. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 2650. 102. Narfstrom, K., Yang, J., Lewis, G., Bragadottir, R., Luna, G., Fisher, S., Whiting, R., Kong, I., Klassen, H. (2010) Development of Müller-like cells after subretinal transplantation of feline red fluorescent retinal progenitors in Abyssinian cat hereditary retinal degeneration. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 3148.

103. Klassen, H. (2010) Progenitor-based delivery of neurotrophic factors for photoreceptor degenerations. XIX Biennial Meeting of the International Society for Eye Research. Abstract number O330. 104. Yang, J., Wang, J., Checa, P., Molina-Martinez, I., R Herrero-Vanrell R., Klassen, H. (2011) Effects of Glial Cell Line-Derived Neurotrophic Factor (GDNF) releasing Poly Lactic-co-Glycolic Acid (PLGA) microparticles on retinal progenitor cells (RPCs). Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 2245. 105. Kamjoo, S., Sharma, V., Yang, J., Checa, P., Molina-Martinez, I., Herrero-Vanrell, R., Limb, G.A., Klassen, H., Kenney, M.C., Kuppermann, B. (2011) Glial Cell Line-Derived Neurotrophic Factor (GDNF) Enriched Poly Lactic-co-Glycolic Acid (PLGA) Microspheres Suppress Apoptosis In Vitro. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract 4443.

106. Yang, J., Klassen, H. (2012) Effects of clinically relevant agents on human retinal progenitor cells (hRPCs) in culture: a pre-clinical cytotoxicity study. Association for Research In Vision and Ophthalmology, Annual Meeting, accessed at www.arvo.org, abstract submitted.