Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113.
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Transcript of Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113.
![Page 1: Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113.](https://reader036.fdocuments.in/reader036/viewer/2022081420/56649ccc5503460f949959ef/html5/thumbnails/1.jpg)
HematopoeisisVIBS 443/602
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Peripheral blood smear (May-Grunwald-Giemsa)113
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Peripheral blood smear (Leishman-Giemsa)eosinophil, basophil, and neutrophils
110
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Bone marrow 1976119761
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Bone marrow smear; low power view, showing negative images of fat globules
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Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA
1. Nuclei of megakaryocyte
2. Red blood cell
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Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation.
1. Macrophage
2. Lymphocyte
3. Neutrophil
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1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
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1. Pronormoblast
2. Basophilic normoblast
3. Polychromatophilic normoblast
4. Orthochromatic normoblast
5. Myelocyte
6. Late metamyelocytes
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1. Late promyelocyte
2. Late myelocyte/early metamyelocyte
3. Nearly mature PMN
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1. Smudge cell
2. Promyelocyte
3. Myelocyte
4. Metamyelocytes
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1. Pronormoblast
2. Metamyelocytes
3. Band cell
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Bone marrow: EM 12a
• Reticular cell
• Developing red blood cell
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Bone marrow; EM 8a
1. Lymphocyte
2. PNM
3. Red blood cell
4. eosinophil
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Bone marrow; EM 12b
1. PMN
2. Immature neutrophil
3. Red blood cell
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Routine H&E of bone
• Marrow spicules
• Lipid in fat cells
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H&E of bone marrow
1. megakaryocytes
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19761
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H&E of bone marrow:
• Red blood cell
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Rib 420
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Rib 420
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Tibia, fetal421
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32583
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32583
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Red bone marrow smear (May-Grunwald-Giemsa)112
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Pronormoblastround nucleus and thin rim of deep blue cytoplasm
Basophilic normoblastnuclear condensation ("fractured nucleus") with a deep blue cytoplasm
Cells of Erythropoisesis
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Polychromatophilic normoblastcytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red
Orthochromatic normoblastmust have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed
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Reticulocyteno nucleus, mature cytoplasm with blue tint
Red blood celltypical of mature cell seen in circulation
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Myelocytecytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape
Cells of Granulopoiesis
Promyelocyteoval nucleus with deep blue cytoplasm and azurophilic granules
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Metamyelocytedeeply indented nucleus with nearly mature cytoplasm (no longer blue)
Band cellnucleus just prior to lobulation with fully mature cytoplasm
Neutrophil (mature)lobulated nucleus with fully mature cytoplasm
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