Protein Synthesis: Transcription and Translation CP Biology.
Heat Shock Factor Protein Family of Transcription Factors
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Transcript of Heat Shock Factor Protein Family of Transcription Factors
Heat Shock Factor Protein Family Heat Shock Factor Protein Family of Transcription Factorsof Transcription Factors
By Brian YuBy Brian Yu
So…What is the question?
What
Leads to the formation of:
What is a Heat Shock Factor Protein?
• It is a group of proteins that are formed when in the presence of heat (also seen when there is some stress to the organism)
• Found in all biological levels.
• Generally, these proteins serve as a way to help other proteins maintain their shape as heat will tend to denature the protein.
Genotyping Gels: At5g62020 At4g11660
General Information about the two genes:
At5g62020 At4g11660
Gene Size (bp)
1555 bp 2076 bp
# amino acids
300 aa 378 aa
#TDNA inserts (actual)
2 2
#TDNA inserts
(predicted)
1 1
Graphical Interpretation of tDNA insert area
5’ UTR Exon Exon 3’ UTR
At5g62020
5’ UTR
At4g11660
Exon Exon 3’ UTR
TDNA insert 5’->3’TDNA 3’->5’
TDNA insert 3’ -> 5’TDNA insert 5’ -> 3’
*not to scale
SALK predicted location (Wrong orientation)
SALK predicted location (wrong orientation)
RT-PCR data and GeneChip data for At5g62020
RT PCR Results for At4g11660
Promoter Region Experiment:
EcoR1 Digest for pAt5g62020
EcoR1 Digest for pAt4g11660
Orientation of the insert in Topo Plasmid (At5g62020)
Topo Plasmid
Promoter RegionSP6 Site
T7 Site5’ 3kB 3’
Any Observable phenotypes in the seed?
WT
Mut
At5g62020
Mut
At4g11660
Observable Phenotypic Variations in the seeds?
WT
Mut
At5g62020
Mut
At4g11660
The future of the experiment
• Sequence the plasmids for the experiment on At4g11660.
• Use the plasmids in further experiments with Green Fluorescence Protein to determine where in the promoter area is the “functional code”