Harvard Systems Biology 200 Handout
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Transcript of Harvard Systems Biology 200 Handout
Scaffold assembly equilibria.
Jerome Feret, Walter FontanaNovember 10, 2007
The simple“star” case: a central hub B with n binding sites for one type of ligand A
1. B is a scaffold with n sites, each of which can bind another protein - or ligand for short - ofjust one type A. Bi is the number of cases in which B has i molecules of A bound to it.
2. The sites of B can be bound in any order.
Question: what is the equilibrium concentration of fully occupied B’s, Bn, as a function of B, thetotal concentration of B in the mixture?
Let na be the number of binding sites available for binding an A molecule. nb is the number ofavailable binding sites that can bind a B. nab is the number of existing bonds between A’s andB’s in the system. This is a local view in terms of sites, rather than agents bearing those sites.With regard to agents, let A and B denote the total number of agents of type A and B,respectively.
We have:
nanb = Kdnab (1)nB = na + nab (2)A = nb + nab (3)
with Kd = k−1/k1. The first equation is the equilibrium condition, the other two equationsexpress site conservation. This allows us to express nab as a function of Kd, A, B, n. In addition,from ligand binding equilibria calculations (see pertinent handout), we have (using currentnotation):
Bn
B=
(nb
Kd + nb
)n
. (4)
nb – the number of free sites (in equilibrium) available for binding to B – is the same as S in thelecture slides or the ligand binding handout of lecture 9.
Combining equations (3) and (4), we obtain:
Bn = B
(A− nab
Kd + A− nab
)n
. (5)
Now we need to get nab. Substituting nb from equation (3) into equation (2), we express na in
1
0 1000 2000 3000 4000
B
0
100
200
300
400
500B n
Figure 1: Star equilibrium. n = 2, A = 1000, Kd = 1. Red: analytical [equations (5) with (8)],black: simulation
terms of nab and constants:
na = Kdnab
A− nab. (6)
Substituting (6) into (3) yields a quadratic equation for nab:
0 = n2ab − nab(Kd + A + nB) + nAB, (7)
with solution
nab =12
((Kd + A + nB)−
√(Kd + A + nB)2 − 4nAB
). (8)
The other solution is physically meaningless, because it violates site conservation.
Using (8) in (5) yields the solution, whose graph is the solid line in Figure 1. The wiggly line ismade of equilibrium ”measurements” of Bn from stochastic simulations (using the KappaFactory).
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