HANDS-ON WORKSHOPS DESCRIPTION

BECKMAN COULTER

LUNCH WORSHOP | Sunday, 22.9., 12:40CytoFLEX – High sensitivity and resolution flow Cytometer – The history, present and the future of the technology CytoFLEX family of Flow Cytometers are known for their extraordinary sensitivity and resolution connected with ease of use and reliability. Some interesting applications to demonstrate the performance will be shown with the future of this cutting-edge technology.

LUNCH WORSHOP | Monday, 23.9., 12:00Dura Innovation – Duraclone, ClearLLab 10 color, Custom design service of dry cocktails of antibodies and it´s performance.Duraclone panels are well known and established on the market for their ease of use, effectiveness and performance. Recently this family has been extended by the ClearLLab 10color panels (CE-IVD) which have moved this technology towards the real Clinical usage. Don´t you find an appropriate design of panels for your purpose? Custom Design Service might be the option. Panel design, sample preparation and data analysis using Clinical Kaluza C software will be shown.

HANDS-ON WORKSHOPS

Sunday, 22.9. 14:35 | Monday, 23.9., 11:00 | Tuesday, 24.9., 9:00

CytoFLEX LX – 6 lasers/21 colors instrument – best in class sensitivity, flexibility and performance for simple analysis even for complex multicolor experiments – introduction, technology, data analysis

Monday, 23.9., 9:00, 14:30 | Tuesday, 24.9., 10:10

Kaluza C – Opened and flexible software platform now certified as CE-IVD – demonstration of analyzing multicolor flow cytometry data (e.g. ClearLLab 10c panels and much more)

LOBBY HALL

Monday, 23.9., 10:20, 15:30

Dura Innovation – come in contact and discuss well-known Duraclone panels (RUO) but also the new ClearLLab 10 color panels (CE-IVD) or Custom Design Service of DRY Multicolor Cocktails for flow cytometry

THERMO FISHER SCIENTIFIC

LUNCH WORSHOP | Sunday, 22.9., 12:40Discover Invitrogen™ EVOS™ M5000 the newest all-in-one Cell Imaging System

Fabrizio Cozzani, Technical Sales Specialist/Thermo Fisher Scientific

The new Invitrogen™ EVOS™ M5000 system integrates precision components with a unique modern design that enables high-quality fluorescence and color imaging with unprecedented flexibility. It is a fully integrated system that combines precision optics, an 18.5“ high-resolution articulated LCD monitor, and a highly sensitive 3.2 MP monochrome CMOS camera (2,048 x 1,536) with 3.45 μm pixel resolution. Designed by biologists for biologists, the EVOS M5000 microscope is remarkably easy to use. Following seamless image acquisition, you can analyze, edit, and annotate your images using a set of convenient tools available in both live mode and for saved images.For common applications, we have created easy-to-use image analysis tools driven by sophisticated segmentation algorithms. With a few clicks you can get a total count of your DAPI-stained cells or an estimate of confluence for reproducibility when you split your cells. Once you have edited and analyzed your images, save the images and data to the embedded hard drive, an external USB device, a local network, or to Thermo Fisher Cloud.

LUNCH WORSHOP | Monday, 23.9., 12:00Next-Generation Flow Cytometry – combining high-speed and precision for improved cell analysis

New developments in cancer research include the detection of rare cells in tissue homogenates and liquid biopsy using the Attune NxT with its unique acoustic focusing technology. In addition, improved assays to characterize immune effector function include cell-based assays to monitor killing of target cells by T cells or antibody-mediated cell killing by Natural Killer cells. We are transitioning these assays into more biologically-relevant models such as whole blood analysis. The

RED HALL

characterization and absolute counting of a range of cell/particle sizes, from very small particles like exosomes or viruses to phenotyping of very large cells, show the transformative performance of the instrument.

HANDS-ON WORKSHOPS

Next-Generation Flow Cytometry – improved assays for cancer research

New developments in cancer research include the detection of rare cells in tissue homogenates and liquid biopsy using the Attune NxT with its unique acoustic focusing technology. In addition, improved assays to characterize immune effector function include cell-based assays to monitor killing of target cells by T cells or antibody-mediated cell killing by Natural Killer cells. We are transitioning these assays into more biologically-relevant models such as whole blood analysis.

Next-Generation Flow Cytometry – Detection of small particles

The characterization and absolute counting of a range of cell/particle sizes, from very small particles like exosomes or viruses to phenotyping of very large cells, show the transformative performance of the Attune NxT flow cytometer

Sunday, 22.9.14:35 Next-generation flow cytometry – improved assays for cancer research

Monday, 23.9.9:00 Next-generation flow cytometry – Attune NxT detection of small particles10:20 Next-generation flow cytometry – improved assays for cancer research14:30 Hands-on Attune NxT performance - focus on whole blood analysis15:30 Hands-on Attune NxT performance - focus on small particles

Tuesday, 24.9.9:00 Hands-on Attune NxT performance - focus on multicolour assays10:10 Hands-on Attune NxT performance - focus on plate assays

BECTON DICKINSON

SUNDAY, 22.3.

Lunch workshop, 12:40 – 14:00 Sorting Brilliant – BD Technology for everyone!Dr. Uwe Speck, Application Specialist Sorter, BD Life Sciences – Biosciences

During the workshop you will be walked through a development of sorting technology in BD. Since the release of BD FACSAriaTM in 2003 the need of cell sorting has become more critical across different laboratory focus. Unsurpassed ease of use, sensitivity, and resolution using lower powered lasers without sacrificing sort performance was transferred into new cell sorter BD FACSMelodyTM to enable the technology to everyone in lab. Contrary to meet the specific needs of highly focused labs BD has developed a program of customized solutions for high parameter cell sorting now introducing BD FACSymphony™ S6 system with up to 9 spatially separated lasers. Come to learn more about 6-way sorting capabilities, ultra-quiet VPX electronics system and index sorting.

Hands-on workshop, 14:35 – 15:10Dri Tubes - see an example of how BD handles the real request for one Dri Multicolor Cocktail.

Mgr. Ondřej Pelák, Application Specialist, BD Life Sciences – BiosciencesMgr. Marcela Vlková, Ph.D., Ústav klinické imunologie a alergologie, FNUSA

BD offers dried products for flow cytometry. Designed for specific experiment requirements, BD Horizon Dri Multicolor Cocktails are pre-aliquoted formats which can reduce variability between experiments, making these reagents ideal choices for multisite or longitudinal studies. A wide variety of reagents and dyes are available for cocktailing, including BD Horizon BrilliantTM dye reagents. BD Horizon Dri Multicolor Cocktails also have an extended shelf life compared to liquid reagents with stability of ≥ 1 year when stored at room temperature. In addition, researchers can easily incorporate these dried down reagents into automated workflow processes, providing greater consistency, precision, standardization and improved lab efficiency. Attend this workshop to see an example of how BD handles the real request for one Dri Multicolor Cocktail and how difficult it may be.

BLUE HALL

MONDAY, 23.9.

Hands-on workshop, 9:00 – 9:30Q&A session with BD Application Specialists.RNDr. Jiří Šinkora, Ph.D., Mgr. Ondřej Pelák, BD Life Sciences – Biosciences

Come to discuss any ideas, concerns or scientific questions related to flow cytometry and cell sorting. Use the opportunity to meet our experts and share with us your experiences.

10:20 – 10:40BD FACSymphonyTM – high-end complex multicolor solution.

Mgr. Ondřej Pelák, Application Specialist, BD Life Sciences – Biosciences

The BD FACSymphonyTM system is a novel cell analyzer that leverages the inherent benefits of flow cytometry and enables the simultaneous measurement of up to 50 different characteristics of a single cell. This high parameter flow cytometer is a powerful analytical tool that enables scientists to identify and analyze distinctive phenotypes in heterogeneous populations. We will show great possibilities with this unique instrument.

11:00 – 11:20BD FACSLyricTM – clinical flow was never that streamlined, now in 12 color configuration.

Mgr. Ondřej Pelák, Application Specialist, BD Life Sciences – Biosciences

Flow cytometry is a powerful tool for the single-cell analysis of patient samples. As clinical flow experiments increase in size and complexity, there is a need for instrument solutions that deliver both a streamlined, user-friendly workflow and exceptional performance. The BD FACSLyricTM flow cytometry solution combines simplicity, speed and automation to ease workflow and improve productivity. This next-generation flow cytometer enables standardization and collaboration through consistent results and unique assay portability capabilities. It is built on a foundation of excellence, experience and expertise. BD FACSLyricTM is a new diagnostic standard for clinical cell analysis, transforming the way your lab does flow cytometry. We will demonstrate that the BD FACSLyricTM system provides unique tools to the clinical user to help achieve reproducible and consistent results across time and instruments.

Lunch workshop, 12:00 – 13:30Colorful particles – next step in flow cytometry detection.

Dr. Tina van den Broeck, Scientific Affairs Scientist, BD Life Sciences – Biosciences

Nowadays we may see that the research is accelerated by uncovering deeper scientific insights across different applications. More often spoken topic is the analysis of smaller particles than cells which are a standard sample type in flow

cytometry. BD keeps an eye on the interest and is working on an additional module enabling Extracellular Vesicle detection. First data were obtained on SORPCelesta and will be shown during the mini-workshop together with ‘tips & tricks’ and some hints to the products to come.

Hands-on workshop, 14:30 – 15:00BD FACSCelestaTM – working together in perfect harmony.

RNDr. Jiří Šinkora, Ph.D., Application Specialist, BD Life Sciences – Biosciences

BD FACSCelestaTM, a compact and cost effective 3-laser and 12 color analyzer with new, low noise VPX electronics will be demonstrated. FACSCelestaTM is driven by BD FACSDivaTM, the gold standard among flow cytometry acquisition software allowing for full control of detectors, automatic and manual compensation, firmware setup changes, ex-post fine tuning of compensation and automatic detector settings standardization using the CS&T bead calibrators and quality control module. Sticking to the basic rules of regular and automatic signal intensity standardization results in preserving the elements of compensation matrix; rerunning single stained compensation controls is recommended once in two months on the BD FACSCelestaTM analyzers. BD FACSCelestaTM configurations will be discussed with the emphasis on UV-equipped devices suited for multicolor brilliant UV polymer dye immunophenotyping having the advantage of lower spectral overlaps and lower sample autofluorescence.

Hands-on workshop, 15:30 – 16:00BD FACSMelodyTM – the most user-friendly cell sorter for everyone.

RNDr. Jiří Šinkora, Ph.D., Application Specialist, BD Life Sciences – Biosciences

BD FACSMelodyTM, a compact, 3-laser and 9 color cell sorter based on the BD FACSAriaTM technology driven by a newly developed, easy to use BD FACSChorusTM software will be demonstrated. BD FACSChorusTM is a user-friendly software allowing for a very fast, step by step daily quality control and drop delay setup procedures that can be skipped on user’s request. As such, the system needs very short training and it can be used by users with almost null knowledge in the field of flow cytometry theory, no operators are needed, which will be demonstrated, too. All formerly operator-requested and/or driven procedures like regular quality control (QC), side stream setup, drop delay definition and cell deposition are based on image analysis and without a need of intervention from the operator side. The use of embedded standardization based on polychromatic CS&T beads and BD patented gain-independent compensation matrix, the feature invented in the most recent pieces of BD software, allow for skipping frequent compensation procedures. Compensation matrix recalculation upon changes of detector gains will also be shown.

TUESDAY, 24.9.

Hands-on workshop, 9:00 – 9:30Q&A session with BD Application Specialists.

RNDr. Jiří Šinkora, Ph.D., Mgr. Ondřej Pelák, BD Life Sciences – Biosciences

Come to discuss any ideas, concerns or scientific questions related to flow cytometry and cell sorting. Use the opportunity to meet our experts and share with us your experiences.

Hands-on workshop, 10:10 – 10:30BD FACSCelestaTM knowledge shoot-out quiz to win 2-months on site demo.

Ing. Tereza Hájková, MBA, BD Life Sciences – Biosciences

As our last mini-workshops we organize a knowledge shoot-out quiz to win BD FACSCelestaTM for your laboratory for 2 months on site demo. Throughout the whole conference you will learn about this flow cytometer platform, use the opportunity and apply all the gathered information about the system in the quiz. Based on the result of knowledge quiz we announce 3 winners (3 different laboratories) with highest number of correct answers.

MILTENYI BIOTEC

LUNCH WORSHOP | Sunday, 22.9., 12:40 MACSQuant® Tyto Cell Sorter

The gentleman among cell sorters.

Microfluidic sorting offers a number of advantages compared to conventional jet-in-air cell sorters. High sample throughput, purity, yield, and low cell stress can be achieved simultaneously. The MACSQuant Tyto Sorter is a benchtop instrument that uses a sterile disposable cartridge with a microfluidic chip to purify samples in a closed environment. At the heart of the microchip is the world’s fastest valve that enables precise flow switching every 10 µs. Therefore, valve actuation rates of up to 30.000 events per second can be achieved. The droplet-free microfluidic approach minimizes cell stress during the sort process, resulting in high cell viabilities and preserved cell functionality. In addition, the close cartridge system prevents aerosol formation, ensuring es-operator safety during the sort process. Simple workflows without complicated setups and alignment procedures allow for sorting with just a few mouse clicks.

HANDS-ON WORKSHOPS

Monday, 23.9., 9:00, 11:00, 15:30 | Tuesday, 24.9., 9:00

MACSQuant® Tyto Cell Sorter

See the MACSQuant® Tyto Cell Sorter in action!

Discover the gentlest way of cell sorting and see benefits coming from increased cell viabilities, contamination-free sorting and increased operator safety. The MACSQuant Tyto Sorter is a benchtop instrument that uses a sterile disposable cartridge with a microfluidic chip to purify samples in a closed environment.

Monday, 23.9., 10:20, 14:30 | Tuesday, 24.9., 10:10

MACSQuant® Analyzer 16

Learn about the MACSQuant® Analyzer 16 automated features!

Come to see a demonstration of the fully automated features of the MACSQuant® Analyzer 16. Explore automated acquisition modes, automated experiment configuration, “smart gains” multi-instrument alignment technology, as well as evaluation of different research applications.

GLASS HALL

miltenyibiotec.com

X

21.-24.9.2019

Hotel Jezerka, Seč-Ústupky, Czech Republic

International Conference Analytical Cytometry

MACS and MACSQuant are registered trademarks of Miltenyi Biotec GmbH. Copyright © 2019 Miltenyi Biotec GmbH. All rights reserved.Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.

research applications.

configuration, multi-instrument alignment technology, as well as evaluation of different

Analyzer 16. Explore automated acquisition modes, automated experiment

Come to see a demonstration of the fully automated features of the MACSQuant®

Learn about the MACSQuant® Analyzer 16 automated features!

viabilities, contamination-free sorting and increased operator safety.

Discover the most gentle way of cell sorting and see benefits coming from increased cell

See the MACSQuant® Tyto Cell Sorter in action!

September 23–24, 2019

Practical hands-on workshops Miltenyi Biotec and Biohem

lunch refreshment will be provided!

Make sure to join us for our lunch session:

Speaker: Dr. Yvonne Diener

sortersThe gentleman among cell

SorterMACSQuant® Tyto Cell

Skleněný salonek12:40-14:00

Sunday, September 22

Lunch workshop

10:10-10:40 MACSQuant® Analyzer 16

09:00-09:30 MACSQuant®Tyto Cell Sorter

Tuesday, September 24

15:30-16:00 MACSQuant®Tyto Cell Sorter

14:30-15:00 MACSQuant® Analyzer 16

11:00-11:30 MACSQuant®Tyto Cell Sorter

10:20-10:50 MACSQuant® Analyzer 16

09:00-09:30 MACSQuant® Tyto Cell Sorter

Monday, September 23

Skleněný salonek

I.T.A. INTERTACT

Lunch workshop, Sunday 22.9., 12:40

Sony ID7000 – The world‘s most powerful cytometer with 7 lasers and 188 detectors

Vendula Šinkorová, Sony Biotechnology

The ID7000 uses spectral technology to collect continuous light from 360 nm to 920 nm using up to 188 detectors, allowing researchers to obtain more data than from any traditional cytometer. With this technology, data can be measured more accurately, and both spectrally adjacent fluorochromes and fluorescent proteins can be used in a panel. The system enables researchers to perform experiments using 44 colors, limited only by the fluorochromes available.

Hands-on workshop, Sunday 22.9., 14:35 | Monday 23.9.2019, 14:30

Sony MA900 – High-end cell sorting has never been so easy

Vendula Šinkorová, Sony Biotechnology

This multi-application sorter offers high level of automation from startup through aseptic cleaning, QC to sort setup (automated drop delay and side stream calibration), all operated by a touch of a button. Siple wizards guide you through all procedures. High level of flexibility includes detection of up to 14 parameters from up to 4 lasers and 4-way sorting into various tube or plate formats. To minimize contamination of your precious samples you can quickly and easily exchange sorting chips, which are automatically loaded and aligned to ensure high level of standardization and reproducibility. Focus on biology instead of spending your time setting and adjusting the sorter!

Hands-on workshop, Tuesday 24.9., 9:00

Sony SA3800 – discover the world of spectral cytometry for your everyday research

Jozef Janda, I.T.A.-Intertact

With a lot of automated features, SA3800 is your perfect companion for your entry into spectral cytometry. Patented spectral technology increases the sensitivity for dim signals by collection of photons from full spectrum from 420 to 800 nm. Spectral unmixing allows combinations of spectrally highly overlapping fluorochromes together in a panel. Multiple autofluorescence populations can be identified and autofluorescence can either be eliminated or studied as a dedicated parameter. Easy to use spectral library eliminates the need to repeatedly measure single stained controls.

YELLOW HALL

Hands-on workshop, Monday 23.9., 9:00

Cytognos - Complete solution for hematology based on Euroflow Next Generation Flow

Jozef Janda, I.T.A.-Intertact

A complete solution from sample preparation and data acquisition to expert-guided automated reporting. Omnicyt™ CE-IVD cytometer’s unique and innovative features make it the perfect complement to work with MM MRD samples following the EuroFlow™ protocols. Its acoustic focusing and the volumetric non-pressurized acquisition system allows for the acquisition of up to 25 million events per file without losing relevant information by electronic abort. Acoustic focusing allows measurement of samples at high sample flow rates (up to 1000 ul/min) without compromising data quility. Infinicyt™ software includes innovative automated data analysis tools that, in combination with the EuroFlow™ reference database, allows a fast identification and characterization of all cell populations.

Hands-on workshop, Monday 23.9., 10:20

Luminex - Immunophenotyping Extracellular Vesicles using modern flow cytometry

Michal Konieczny, Luminex

Only recently has the importance of extracellular vesicles (EVs) as key mediators of intercellular communication been appreciated. EVs are membrane derived structures that include exosomes, microvesicles and apoptotic bodies. Exosomes have been shown to transfer molecules between cells and have the potential to transfer signals between cells. Quantifying and characterizing EVs in a reproducible and reliable manner has been difficult due to their small size (exosomes range from 30 – 100 nm in diameter). Attempts to analyze EVs using traditional PMT based flow cytometers has been hampered by the limit of detection of such small particles and low refractive index. To overcome these limitations we have employed ImageStreamX imaging flow cytometer and Luminex recently developed CellStreamTM flow cytometer. The CellStreamTM utilizes the Amnis® imaging technology, having the advantage of high throughput flow cytometry with higher sensitivity to small particles due to the time-delay-integration image capturing system. Join us during the lecture and discover the potential of flow cytometers that provides unparalleled flexibility and performance.

Lunch workshop, Monday 23.9., 12:00

Luminex - High speed, high content & ultra-high sensitivity Imaging Flow Cytometry analysis and next generation image processing

Michal Konieczny, Luminex

By combining the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insight of microscopy, the Amnis® ImageStream®X Mk II and Amnis® FlowSight® Instruments overcome the

limitations of both techniques and open the door to an extensive range of novel applications. Ability of collecting spectrally separated images for a large number of cells together with next generation image processing strategies incorporating machine learning and deep learning algorithms leads to unrevealed insights in cellular forms and functions. Join us during the workshops and discover the potential of Imaging Flow Cytometers

IBA LIFESCIENCES

Hands-on workshopSunday 22.9., 14:35Monday 23.9., 10:20, 11:00, 14:30Tuesday 24.9., 10:10

Fab-TACS® - cell separation reinvented

Dr. Claudia Wrzos, Product Manager Cell Selection & Expansion, IBA Lifesciences

Finding a suitable method for cell separation can be challenging. The method should preserve the biology of targeted cells rather than only the population of certain cell types. In our workshop you will learn all about our automated and manual cell separation solutions for your immunological or cell biological applications. The technology is based on an affinity chromatography system for non-magnetic isolation of immune cells. It works with Twin-Strep-tagged Fab fragments, which reversibly capture and release the target cells. The Fab-TACS® technology delivers label-free, non-activated cells, making them perfectly suitable for demanding immunological or cell biological uses.We hope to see you in the workshop!

GREEN HALL

ACCELA

Hands-on workshop, Sunday 21.9., 14:35

Spectral Cytometry with the Cytek Aurora – New Approaches in the use of highly overlapping dyes for flow cytometryRecent advances in instrumentation and the development of polymer dyes have resulted in a dramatic increase in the ability to design and perform multicolour assays in flow cytometry. Spectral flow cytometers, such as the Cytek Aurora, provide the ability to utilise highly overlapping dyes with no detrimental effect to assay sensitivity thereby increasing the options of dyes that can be used in combination. This workshop will present data from 3 colour and 16 colour leucocyte panels that effectively identify T cell subsets stained with highly overlapping dyes including: BV421, Super Bright 436 and BV510. The spectrum signatures of these highly overlapping dyes will be analysed and compared, and a multicolor analysis with spectral unmixing will be demonstrated

Workplan This workshop will utilise the 3-colour an 16 colour demo panel of pre-stained Lyophilised PBMCs have been previously run and then analysed on the day through offline analysis of pre-recorded data through a projector. Slides of the dye spectra also be displayed to show how conventional cytometry is limited in its ability to discriminate the BV421 and SB436. This walkthrough could be rapidly achieved in a 25-minute window particularly from a purely data analysis approach with pre-designated templates. This could even be expanded onto the 16-colour panel if determined as a more appropriate approach to display the capabilities of the Aurora.

Hands-on workshop, Monday 22.9., 9:00

Spectral Cytometry with the Cytek Aurora – Hide & Seek: Using Autofluorescence Extraction (AFE) to Better Resolve Your Population of InterestQuestion – Is your dim population proving difficult to see? Spectral flow cytometry empowers users experiencing high autofluorescence to treat the autofluorescence as its own fluorescence tag during unmixing allowing them to now easily resolve dim or poorly separated populations. Do you see a way that autofluorescence extraction (AFE) will be able to help your flow cytometry experiment? Experimental Plan – To best understand how AFE can improve the resolution of hard to see populations, we utilize large highly auto fluorescent human myeloid GFP+ and GFP- K562 cells. Gating only on live cells using a viability dye, we demonstrate unmixing with and without AFE to look at the resolution of GFP+ cells from GFP- cells by extracting out the background autofluorescence of the GFP- population used in our unstained

COLOUR ROOM

control.Workplan The plan for this would be to utilise the workshop data that our client has set up recently this year for her flow course. The approach consist of a guided analysis to show how the data looks with and without autofluorescence extract to demonstrate how AFE can result in improved resolution of populations and is data analysis implications.

Hands-on workshop, Monday 22.9., 10:20

Spectral Cytometry with the Cytek Aurora – Taste the Rainbow: Comparing two fluorochromes, how do we tell if signatures are unique?Question – What is a spectral signature in spectral flow cytometry how does one determine if a fluorochrome spectral signature is “unique enough” to use in combination with others? What can be done when two signatures are visually very similar to each other?Experimental Plan – This workshop will demonstrate data from two samples of Antibody Capture (AbC) beads stained with different reagents that have been run on the Aurora. The median fluorescence from each channel will be used to compare signatures to determine possible overlap utilising our spectral overlap tool for Excel. Based on overlap in the collected spectra we will demonstrate the practicality of using these dyes in combination together in the same sample. Are they unique enough? If the user decides to use these in combination together, what considerations need to be made during panel design?

Hands-on workshop, Monday 22.9., 14:30

See what you‘ve been missing - workshop on unique data analysis generated on Imaging Flow Cytometer Imaging Flow Cytometry (IFC) combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the Luminex ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system’s imaging abilities to locate and quantitate the distribution of signals on in or between cells.

Hands-on workshop, Tuesday 23.9., 9:00

The new CellStream – Benchtop flow cytomter with unparelleled sensitivity and flexibility for cell and small particles analysisThe new Luminex CellStream Benchtop Flow Cytometer delivers unparalleled sensitivity and flexibility for cell and small particles analysis. This compact system contains patented optics technology unique to our state-of-the-art Amnis flow cytometers and has a fully configurable 7-laser capacity. With highly sensitive and configurable optics, researchers benefit from multiparameter detection capabilities, while maintaining the flexibility to tailor and expand the system according to their research needs and budget. The CellStream is a highly-customizable and compact flow cytometer that is the first to use a camera for detection. Only recently has the importance of extracellular vesicles as key mediators of intercellular communication been appreciated. Attend this workshop to learn more about how the unique Amnis time delay integration and camera technology inside the CellStream System enables immunophenotyping of extracellular vesicles.