Haematology and Blood Transfusion 26-------------------------------Hämatologie und Bluttransfusion

Edited by H. Heirnpel, Ulrn . D. Huhn, München C. Mueller-Eckhardt, Gießen G. Ruhenstroth-Bauer, München

Modern Trends in Human Leukemia IV Latest Results in Clinical and Biological Research Including Pediatric Oncology

Organized on behalf of the Deutsche Gesellschaft für Hämatologie und Onkologie and the Deutsches Krebsforschungszentrum. Wilsede, June 16-19, 1980

Wilsede Joint Meeting on Pediatric Oncology I Hamburg, June 20/21, 1980

Editedby R. Neth, R. C. Gallo, T. Graf, K. Mannweiler and K. Winkler

With 252 Figures and 118 Tables

Springer-Verlag Berlin Heidelberg NewYork 1981

Dr. Rolf Neth, Universitäts-Kinderklinik, Molekularbiologisch-hämato­logische Arbeitsgruppe, Martinistraße 52, D-2000 Hamburg 20, Federal Republic of Germany

Dr. Robert C. Gallo, National Cancer Institute, Laboratory of Tumor Cell Biology, Bethesda, Maryland 20014, USA

Dr. Thomas Graf, Institut für Virusforschung, Im Neuenheimer Feld 280, D-6900 Heidelberg 1, Federal Republic of Germany

Dr. Klaus Mannweiler, Heinrich-Pette-Institut für Experimentelle Viro­logie und Immunologie an der Universität Hamburg, Martinistraße 52, D-2000 Hamburg 20, Federal Republic of Germany

Dr. Kurt Winkler, Universitäts-Kinderklinik, Abteilung für Haematolo­gie und Onkologie, Martinistr. 52, D-2000 Hamburg 20, Federal Republic of Germany

Supplement to

BLUT - Journal Experimental and Clinical Hematology

Organ of the Deutsche Gesellschaft für Hämatologie I1nd Onkologie der Deutschen Gesellschaft für Bluttransfusion und Immunohämatologie and of the Osterreichi­schen Gesellschaft für Hämatologie und Onkologie

ISBN-13: 978-3-540-10622-7 e-ISBN-!3: 978-3-642-67984-1 DOI: I 0.1 007/ 978-3-642-67984-1

This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned specifically those of translation, reprinting, re-use of illustrations, broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Lawwhere copies are made for other than private use a fee is payable to "Verwertungsgesell­schaft Wort", Munich.

© Springer-Verlag Berlin Heidelberg 1981

The use of registered names, trademarks etc. in this publication does not imply even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.

2127/3321/543210

Scientilic Organisation of the Sessions

Clinical Aspects

Frei, Emil II!, Sidney Farber Cancer Institute, 44, Binney Street, Boston, MA 02115, USA Lister, Andrew, Dept. Medical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, U.K.

Cytogenetic and Lymphomas

Kaplan, Henry S., Dept. Radiology, Cancer Biology Research Laborato­ry, Stanford University Medical School, Stanford CA 94305, USA Klein, George, Dept. Tumor Biology, Karolinska Institutet 104 Stock­holm 60, Sweden

Cell Biology

Greaves, Mel F., Imperial Cancer Research Fund Laboratories P.O. Box 123, Lincoln's Inn Fields, London WC2A3PX, u.K. Moore, MaJcolm, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Immunology

Eichmann, Klaus, Krebsforschungszentrum - Immunologie, Im Neuen­heim er Feld 280, 6900 Heidelberg, West Germany Mitchison, Nicholas Avrion, Tumor Immunology Unit, University College London, Gower Street, London WCIE6BT, U.K.

Virology and Molecular Biology

Duesberg, Peter, Dept. Molecular Biology, WendeIl M. Stanley Hall, University of California, Berkeley, CA 94720, USA Gallo, Robert c., National Cancer Institute, Bethesda, MD 20014, USA

Wilsede Joint Meeting on Pediatric Oncology I

Kabisch, Hartmut, Universitätskinderklinik, Abt. für Hämatologie und Onkologie, Martinistr. 52, 2000 Hamburg 20, West-Germany Winkler, Kurt, Universitätskinderklinik, Abt. für Hämatologie und Onkologie, Martinistr. 52, 2000 Hamburg 20, West-Germany

v

Contents

Participants of the Meeting .. . . . . . . . . . . . . . . . . . . . . . . . . . . .. XV Wilsede Scholarship Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . .. XIX Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. XXI Acknowledgement .................................... XXV

Frederick Stohlman Jr. Memorial Lecture

Klein, G.: The Relative Role of Viral Transformation and Specific Cytogenetic Changes in the Development of Murine and Human Lymphomas ......................................... 3

Kaplan, H. S.: On the Biology and Immunology of Hodgkin's Disease . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

Clinical Aspects

Rowley, J. D.: Chromosome Studies in Children and Adults with Leukemia1 ••••.••••••..••••......•••••••.•....•..... 27

Georgii, A., Thiele, J., Vykoupil, K.-F.: Myelqid Dysplasia: the Histopathology of Preleukemia . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

Lister, T. A., Johnson, S. A. N., Bell, R., Henry, G., Malpas, J. S.: Progress in Acute Myelogenous Leukemia .................. 38

Mayer, R. J., Weinstein, H. J., Rosenthai, D. S., Coral, F,'S., Nathan, D. G., Frei, E. III.: VAPA10 : A TreatmenfProgram for Acute Myelocytic Leukemia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Preisler, H. D.: Rational Approaches to the Treatment oi Leukemia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Moloney, W. c., Rosenthai, D. S.: Treatment of Early Acute Nonlymphatic Leukemia with Low Dose Cytosine Arabinoside .. 59

Rohatiner, A., Balkwill, F., Malpas, J.S., Lister, T. A.: A Phase I Study of Human Lymphoblastoid Interferon in Patients with Hematological Malignancies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

VII

Mertelsmann, R., Moore, M. A. S., Clarkson, B.: Methods and Clinical Relevance of Terminal Deoxynuc1eotidyl Transferase Determination in Leukemie Cells2 ........................ 68

Hiddemann, W., Andreeff, M., Clarkson, B. D.: Quantitation of Chemotherapy-Induced Cytoreduction in Acute Leukemia2 . . . . . 73

Miller, D. R., Leikin, S., Albo, V., Sather, H., Karon, M., Hammond, D.: Intensive Therapy and Prognostic Factors in Acute Lymphoblastic Leuk~mia of Childhood: CCG 141. AReport from Childrens Cancer Study G roup2 .......................... 77

Henze, G., Langermann, H.-J., Ritter, J., Schellong, G., Riehm, H.: Treatment Strategy for Different Risk Groups in Childhood Acute Lymphoblastie Leukemia: AReport From the BFM Study G roup2 ............................................. 87

Rivera, G.: Recurrent Childhood Lymphocytie Leukemia: Outco-me of Marrow Relapses After Cessation of Therapy2 . . . . . . . . . . . 94

Sinks, L. F., Wang, J. J., Freeman, A. 1.: TheTreatmentofPrimary Childhood Acute Lymphocytic Leukemia with Intermediate Dose Methotrexate2 ....................................... 99

Chessells, J. M., Ninane, J., Tiedemann, K.: Present Problems in Management of Childhood Lymphoblastie Leukaemia: Experien-ce from the Hospital for Siek Children, London2 .............. 108

Zintl, F., Plenert, W., Hermann, J.: Different Therapy Protocols for High Risk and Standard Risk ALLin Childhood ........... 115

Sallan, S. E.: T -Cell Acute Lymphoblastic Leukemia in Child-ren2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 121

Rodt, H., Thierfelder, S., Kolb, H. J., NetzeI, B., Haas, R J., Wilms, K., Bender-Götze, C.: The Concept of GvHD-Suppression by In Vitro Treatment with Antisera2 . . . . . . . . . . . . . . . . . . . . . .. 124

Storb, R.: Bone Marrow Transplantation for the Treatment of Leukemia . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 136

Morgenstern, G. R, Powles, R L.: Allogeneic Bone Marrow Transplantation for Acute Myeloid Leukemia in First Remission.. 139

Furusawa, M., Iino, T.: Use of Erythrocyte Ghosts forTherapy . .. 143

Hardesty, B., Henderson, A. B., Kramer, G.: A Possible Bioche­mieal Basis for the Use of Hyperthermia in the Treatment of Cancer and Virus Infection .............................. 146

Cytogenetic Aspects of Lymphomas

Rowley, J. D.: The Implications of Nonrandom Chromosome Changes for Malignant Transformation . . . . . . . . . . . . . . . . . . . .. 151

VIII

Carrano, A. v., Lebo, R V., Yu, L. c., Kan, Y. W.: Regional Gene Mapping of Human Chromosomes Purified by Flow Sorting ..... 156

Levan, G., Mitelman, F.: The Different Origin of Primary and Secondary Chromosome Aberrations in Cancer .............. 160

Cline, M. J., Stang, H. D., Mercola, K, Salser, W.: Insertion of NewGenesintoBoneMarrowCellsofMiee ................. 167

Scheer, D. 1., Srimatkandada, S., Kamen, B. A., Dube, S., Bertino, J. R: Organization of the Methotrexate Resistant Mouse L5178YR Dihydrofolate Reductase Gene and Transformation of HumanHCT-8CellsbyThisGene ........................ 171

Hauser, H., Graf, T., Beug, H., Greiser-Wilke, 1., Lindenmaier, W., Grez, M., Land, H., Giesecke, K, Schütz, G.: Structure of the Lysozyme Gene and Expression in the Oviduct and Macropha-ges ................................................ 175

Zeuthen, J., Klein, G.: Some Recent Trends in Studies of Human Lymphoid Cells: B-Cells, Epstein-Barr Virus, and Transforma-tion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 179

Wolf, H., Wilmes, E., Bayliss, G. J.: Epstein-BarrVirus: Its Siteof Persistence and Its Role in the Development of Carcinomas ..... 191

Kaschka-Dierich, c., Bauer, 1., Fleckenstein, B., Desrosiers, R C.: Episomal and Nonepisomal Herpesvirus DNA in Lymphoid Tumor Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 197

Brade, L., Gissmann, L., Mueller-Lantzsch, N., zur Hausen, H.: B-Lymphotropic Papovavirus (LPV) - Infections of Man? ...... 204

Purtilo, D. T.: Immunopathology of the X-Linked Lymphoprolife-rative Syndrome ...................................... 207

Nilsson, K., Forsbeck, K., Gidlund, M., Sundström, c., Tötterman, T., SälIström, J., Venge, P.: Surface Characteristies of the U-937 Human Histiocytic Lymphoma Cell Line: Specific Changes During Inducible Morphologie and Functional Differentiation In Vitro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 215

Radzun, H. J., Parwaresch, M. R., Lennert, K: Enzyme Polymor­phism as a Biochemical Cell Marker: Application to the Cellular Origin and Homogeneity of Human Macrophages and to the Classification of Malignant Lymphomas .................... 222

Wielckens, K, Garbrecht, M., Schwoch, G.,Hilz, H.: Human Leukemie Lymphocytes - Biochemical Parameters of the Altered Differentiation Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 225

Diehl, V., Kirchner, H. H., Schaadt, M., Fonatsch, c., Stein, H.: Lymphoproliferation and Heterotransplantation in Nude Miee: Tumor Cells in Hodgkin's Disease . . . . . . . . . . . . . . . . . . . . . . . .. 229

IX

Cell Biological and Immunological Aspects

Moore, Mo Ao So: Geneticand Oncogenic Influences on Myelo-poiesis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 237

Broxmeyer, Ho Eo, Bognacki, Jo, Dörner, Mo Ho, deSousa, Mo, Lu, L.: Acidic Isoferritins as Feedback Regulators in Normal and Leukemic Myelopoiesis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 243

Messner, Ho Ao, Fauser, Ao Ao, Buick, Ro, Chang, L. Jo-Ao, Lepine, Jo, Curtis, Jo Co, Senn, Jo, McCulloch, Eo A.: Assessment of Human Pluripotent Hemopoietic Progenitors and Leukemic Blast-For-ming Cells in Culture 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 246

Afanasiev, B. Vo, Elstner, Eo, Saidali, Mo Ao, Zabelina, To So: Proliferation and Maturation of Hemopoietic Cells in Adult Patients with Different Forms of Acute Leukemia and Chronic M yeloid Leukemia in Agar and Liquid Cultures 0 0 0 0 0 0 0 0 0 0 0 0 0 0 251

Burk, Eo, Chennaoui-Antonio, L., Beiersdorf, Ho, Hellwege, Ho Ho, Winkler, Ko, Heinisch, Bo,Küstermann, Go, Krause, Uo, Kitschke, Ho Jo, Voigt, Ho, Neth, R.: Cytological and Cytochemical Analysis of Plasma Clot Cultures and Peripheral Granulocytes Related to Long Term Survival in Childhood ALL and Cancer Patients UnderCytostatica Therapy 000 0 0 0 000 0 000 0 0 0 0 000 0 0 0 255

Hoelzer, Do, Harriss, Eo Bo, Carbonell, Fo: Maturation of Blast Cells in Acute Transformation of Chronic Myeloproliferative Syndrome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 261

Lau, B., Jäger, Go, Thiel, Eo, Pachmann, K., Rodt, Ho, Thierfelder, So, Dörmer, Po: Patterns of Cell Surface Differentiation of cALL Positive Leukemic Blast Cells in Diffusion Chamber Culture 0 0 0 0 0 265

Forbes, Po, Dobbie, Do, Powles, Ro, Alexander, Po: Maturation of Human Peripheral Blood Leukemic Cells in Short-Term Culture 0 0 268

Dexter, To Mo: Regulator-Dependent Hemopoiesis and Hs Possi-ble Relevance to Leukemogenesis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 272

Gartner, So, Kaplan, Ho So: Long-Term Culture of Normal and LeukemicHumanBoneMarrow 00000000000000000000000000 276

Greenberg, Ho Mo, Parker, L. Mo, Newburger, Po Eo, Said, Jo, Cohen, Go 1., Greenberger, Jo So: Corticosteroid Dependence of Continuous Hemopoiesis In Vitro with Murine or Human Bone Marrow 000000000000000000000000000000000000000000000 289

Mitchison, No A.: Cloning Cells ofthe Immune System 0 0 0 0 0 0 0 0 0 294

Greaves, Mo Fo, Robinson, Jo Bo, Delia, Do, Ritz, Jo, Schlossman, So, Sieff, Co, Goldstein, Go, Kung, Po, Bollum, Fo J 0' Edwards, Po A. Wo: Comparative Antigenic Phenotypes of Normal and Leukemic Hemopoietic Precursor Cells Analysed with a "Library" of Monoclonal Antibodies2

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 296

x

van den Engh, G., Trask, B., Visser, J.: Surface Antigens of Pluripotent and Committed Haemopoietic Stern Cells . . . . . . . . .. 305

BeverIey, P. C. L., Linch, D., Callard, R. E.: Human Leucocyte Antigens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. 309

Godal, T., Lindrno, T., Ruud, E., Heikkilä, R., Henriksen, A., Steen, H. B., Marton, P. F.: Immunologic Subsets in Human B-Cell Lymphomas in Relation to Normal B-Cell Development .. 314

Hengartner, H.: Clones ofMurine Functional TCells .......... 320

Minowada, J.: Marker Profiles of Leukemia-Lymphoma Cell Lines2 . . . • . . . • . . . • . . . . . • • . . . . . . . • • • . . . . . . . • • . . . . . . .. 323

Newman, R. A., Sutherland, D. R., Greaves, M. F.: Biochemical Characterization of an Antigen Associated with Acute Lympho­blastic Leukemia and Lymphocyte Precursors2 ...........•... 326

Becker, W.-M., Schmiegel, W.-H., Kabisch, H., Arndt, R., Thiele, H.-G.: SerologicSubtypingofcALL2 ...................... 329

Baker, M. A., Roncari, D. A. K., Taub, R. N., Mohanakumar, T., Falk, J. A.: Acute Myeloblastic Leukemia-Associated Antigens: Detection and Clinicallmportance2 ....................... 332

Andersson, L. c., von Willebrand, E., Jokinen, M., Karhi, K. K., Gahmberg, C. G.: Glycophorin A as an Erythroid Marker in Normal and Malignant Hematopoiesis2 . . . . . . . . . . . . . . . . . . . .. 338

Eichmann, K.: The Network Concept and Leukemia . . . . . . . . . .. 343

Klein, E.: Interpretation and In Vivo Relevance of Lymphocyto-toxicity Assays ....................................... 345

Milleck, J., Jantscheff, P., Thränhardt, H., Schöntube, M., Gürtler, R., Seifart, D., Pasternak, G.: Acute Leukemia, and Nonspecific Killer Cells .......................................... 351

Teodorescu, M., Hsu, C., Bratescu, A., DeBoer, K. P., Nelson, R., Kleinman, R., Wen, C. M.-J., Mayer, E. P., Pang, E. J.-M.: The Use of Bacteria as Markers of Leukemic Lymphocytes and for the Isolation of Natural Killer Cells ........................ _'.. 355

McGrath, M. S., Jerabek, L., Pillemer, E., Steinberg, R. A., Weissman, I. L.: Receptor Mediated Murine Leukemogenesis: Monoclonal Antibody Induced Lymphoma Cell Growth Arrest 360

Stoye, J. P., Moroni, c.: Effect of Brombdeoxyuridine on Endogenous Retrovirus Production in Differentiating Murine Lymphocytes ........................................ 365

Schreiber, H., Flood, P. M., Kripke, M. L., Urban, J. L.: Modulation of Growth of Malignant Cells by Anti-Idiotypic Immunity ........................................... 368

XI

Kyewski, B., Hunsmann, Go, Friedrich, R., Ketelsen, Uo-Po, Wekerle, Ho: Thymic Nurse Cells: Intraepithelial Thymocyte Sojourn and Its Possible Relevance for the Pathogenesis of AKR Lymphomas 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 372

Schirrmacher, Vo, Bosslet, Ko: Generation of Stable Antigen Loss Variants from Cloned Tumor Lines - An Example of Immuno­adaptation During Metastasis 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 377

Virological and Molecularbiological Aspects

Duesberg, Po Ho, Bister, Ko: Transforrning Genes of Retroviruses: Definition, Specificity, and Relation to Cellular DNA 0 0 0 0 0 0 0 0 0 0 383

Erikson, Eo, Erikson, R. L. : A Cellular Protein Phosphorylated by the Avian Sarcoma Virus Transforming Gene Product 0 0 0 0 0 0 0 0 0 0 397

Friis, R. R., Ziemiecki, Ao, Bosch, Vo, Boschek, C. Bo, Bauer, Ho: Correlated Loss of the Transformed Phenotype and pp60src-Asso-ciated Protein Kinase Activity 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 402

Moelling, K., Owada, Mo K., Donner, Po, Bunte, To: The Transformation-Specific Protein pp60src from an A vian Sarcoma Virus 00000000000000000000000000000000000000000000000 405

Konze-Thomas, Bo, von der Helm, K.: Proteolytic Processing of Avian and Simian Sarcoma and Leukemia Viral Proteins 0 0 0 0 0 0 0 0 409

Kramer, Go, Grankowski, No, Hardesty, Bo: Control of Protein Synthesiso Phosphorylation and Dephosphorylation of Eukaryotic Peptide Initiation Factor 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 414

Graf, To, Royer-Pokora, Bo, Korzeniewska, Eo, Grieser, So, Beug, Ho: Characterization of the Hematopoietic Target Cells of Defecti-ve Avian Leukemia Viruses by Velocity Sedimentation and Density Gradient Centrifugation Analyses 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 417

Vogt, Po K., Neil, Jo c., Moscovici, c., Breitman, Mo L.: PRCII, a Representative of a New Class of Avian Sarcoma Virus es .0 0 0 0 0 0 424

Souza, L. Mo, Bergmann, Do Go, Baluda, Mo Ao: Identification of theAvianMyeloblastosisVirusGenome 0 0 0 0 0 0 0 0 0 0 0 000000000 429

Coffin, Jo Mo, Tsichlis, Po No, Robinson, Ho L.: Genetics of Leukemogenesis by Avian Leukosis Viruses 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 432

Hayward, Wo So, Neel, B. Go, Fang, Jo, Robinson, Ho L., Astrin, So Mo: Avian Lymphoid Leukosis is Correlated with the Appear­ance of Discrete New RNAs Containing Viral and Cellular Genetic Information 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 439

Kung, Ho Jo, Fung, Yo K., Crittenden, L. Co, Fadly, A., Dube, So K.: The Molecular Basis of Avian Retrovirus-Induced Leukemogene-sis 000 0 0 0 0 0 0 0 0 000 0 0 0 0 0 0 0 000 000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 445

XII

Löliger, Ho-C., von dem Hagen, Do, Hartmann, Wo: Studies of the Association of Leukemogenic and Oncogenic Properties in Avian Leukemia Viruses 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 452

Aaronson, So Ao, Barbacid, Mo, Dunn, C. Yo, Reddy, Eo Po: Genetic Approaches Toward Elucidating the Mechanisms of Type-C Virus-InducedLeukemia 0000000000000000000000000000000 455

Blair, Do Go, Oskarsson, Mo, McClements, Wo L., Vande Woude, Go Fo: The Long Terminal Repeat of Moloney Sarcoma Provirus Enhances Transformation 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 460

Waneck, Go L., Rosenberg, No: Ontogeny of Abelson Murine Leukemia Virus Target Cells 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 467

Evans, L. Ho, Duesberg, Po Ho, Linemeyer, Do L., Ruscetti, So Ko, Scolnick, Eo Mo: Structural and Functional Studies of the Friend Spleen Focus-Forming Virus: Structural Relationship of SFFV to Dualtropic Viruses and Molecular Cloning of a Biologically Active SubgenomicFragmentofSFFVDNA 0000000000000000000000 472

van den Berg, Ko Jo, Krump-Konvalinkova, Vo, Bentvelzen, Po: Enhancing Effect of Murine Leukemia Virus on Fibroblast Transformation by Normal BALB/C Mouse DNA Fragments 0 0 0 479

Essex, Mo, Grant, Co K., Cotter, So Mo, Hardy, Wo Do Jr.: Role of Viruses in the Etiology of Naturally Occuring Feline Leukemia 0 0 0 483

Snyder, Ho Wo Jr., Dutta-Choudhury, Mo, Hardy, Wo Do Jr.: Relationship of the Feline Oncornavirus Associated Cell Membra-ne Antigen to a Feline Sarcoma Virus Encoded Polyprotein 0 0 0 0 0 488

Hardy, Wo Do Jr., McClelland, Ao Jo, Zuckerman, Eo Eo, Snyder, Ho Wo Jr., MacEwen, Eo Go, Francis, Do, Essex, Mo: Feline Leukemia Virus Nonproducer Lymphosarcomas of Cats as a Model for the EtiologyofHumanLeukemias 000000000000000000000000000 492

Kettmann, Ro, Marbaix, Go, Mammerickx, Mo, Burny, Ao: Geno-mic Integration of Bovine Leukemia Provirus and Lack of Viral RNA Expression in the Target Cells of Cattle with Different Responses to BLV Infection 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 495

Portetelle, Do, Bruck, c., Mammerickx, Mo, Burny, Ao: Natural Antibodies to BLV gp51 Are Reactive Against the Carbohydrate Moiety ofthe Glycoprotein 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 498

Gallo, R. c., Poiesz, B. Jo, Ruscetti, F. Wo: Regulation of Human T -Cell Proliferation: T -Cell Growth Factor and Isolation of a New Class of Type-C Retroviruses from Human T -Cells 0 0 0 0 0 0 0 0 0 0 0 0 502

Reitz, Mo So Jr., Poiesz, B. Jo, Ruscetti, Eo Wo, Gallo, R. Co: Characterization by Nucleic Acid Hybridization of HTLV, a Novel Retrovirus from Human Neoplastic T -Lymphocytes 0 0 0 0 0 0 0 0 0 0 0 515

XIII

Torelli, U., Torelli, G., Narni, F., Donelli, A., Ferrari, S., Franchini, G., Calabretta, B.: Different Frequency Classes of Sequences in Heterogeneous Nuc1ear RNA of Normal Promyelocytes and Lymphoblasts and of Leukemic Blast Cells of Circulating Blood and of the HL60 Line .................................. 517

Thiel, H.-J., Matthews, T., Broughton, E., Butchko, A., Bolognesi, D.: Characterization of Antigens in SSV Nonproducer Cells . . . .. 520

Bergholz, C. M.: Viral Gene Expression in Cells Transformed by Simian Sarcoma Virus, an Infectious Primate Type C Retrovirus .. 524

Jore, J., Dubbes, R., Coolen, J., Nooter, K.: Cell Fusion as a Tool for the Detection of Viral Footprints in Childhood Leukemia .... 527

Hehlmann, R., Schetters, H., Erfle, V.: ELISA for the Detection of Antigens Cross-Reacting with Primate C-Type Viral Proteins (p30, gp70) in Human Leukemic Sera . . . . . . . . . . . . . . . . . . . . .. 530

Erfle, V., Hehlmann, R., Schetters, H., Schmidt, J., Luz, A.: Radiation-Induced Murine Leukemias and Endogenous Retro­viruses: The Time Course of Viral Expression . . . . . . . . . . . . . . .. 537

Löwer, J., Löwer, R., Stegmann, J., Frank, H., Kurth, R.: Retrovirus Partic1e Production in Three of Four Human Teratocar­cinomaCeliLines .,................................... 541

General Summary

Deinhardt, F.: General Summary 547

Subject Index ........................................ 551

1 Speciallecture far the Wilsede Joint Meeting on Pediatric Oncology I 2 Were also presented in the Wilsede Joint Meeting on Pediatric Oncology I

XIV

Participants of the Meeting

Aaronson, A., Laboratory of RNA Tumor Viruses, National Cancer Institute, Bethesda, MD 20014, USA

Andersson, L., Transplantation Laboratory, University of Helsinki, Haartmanin­katu 3A, 00290 Helsinki 29, Finland

Andreeff, M., Memorial Sloan-Kettering Cancer Center, 1275 Y ork Avenue, New York, NY 10021, USA

Baluda, M. A., UCLA Cancer Center, Dept. Microbiology and Immunolgy, 921 Westwood Boulevard, Los Angeles, CA 90024, USA

Barbacid, M., Laboratory of RNA Tumor Viruses, National Cancer Institute, Bethesda, MD 20014, USA

Beck, J. D., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Bentvelzen, P., Radiobiological Institute, 151 Lange Kleiweg, Rijswijk, The Netherlands

Bertino, J., Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA

Beverley, P. C. L., ICRF Human Tumour Immunology Group, University of London, University Street, London WC1E6JJ, U.K.

Bister, K., Dept. Molecular Biology, Wendel! M. Stanley Hall, University of Califomia, Berkeley, CA 94720, USA

Brade, Lore, Institut für Virologie im Zentrum für Hygiene, Hermann-Herder­Straße 11,7800 Freiburg i. Br., West-Germany

Bumy, A., Faculte des Sciences Agronomiques de l'Etat, Chaire de Zootechnie, Gembloux, Belgium '

Carrano, A., Biomedical Division, L-452, Lawrence Livermore Labs. Livermore, CA 94550, USA

Chermann, J.-C., Institut Pasteur, 25, Rue du Docteur Roux, 75724 Paris, Cedex 15, France

Cline, M., UCLA Center for Health Sciences, Dept. Medicine, Los Angeles, CA 90024, USA

Coffin, J. M., Dept. Molecular Biology, Tufts University, 1.36 Harrisbn Avenue, Boston, MA 02111, USA

Deinhardt, F., Max v. Pettenkofer-Institut, Pettenkoferstraße 9a, 8000 München 2, West-Germany

Dexter, T. M., Christie Hospital & Holt Radium Institute, Paterson Laboratories, Manchester M20 9BX, U.K.

Diehl, V., Medizinische Hochschule Hannover, Depärtment für Innere Medizin, Karl-Wiechert-Allee 9, 3000 Hannover-Kleefeld, West-Germany

Duesberg, P., Dept. Molecular Biology, Wendel! M. Stanley HaI!, University of Califomia, Berkeley, CA 94720, USA

Eichmann, K., Krebsforschungszentrum - Immunologie, Im Neuenheimer Feld 280, 6900 Heidelberg, West-Germany

Elstner, Elena, Abt. Zellkinetik, Zentralinstitut für Molekularbiologie, Linden­berger Weg 70, 1115 Berlin-Buch, GDR

xv

van den Engh, G., Radiobiological Institute 151 Lange Kleiweg, Rijswijk, The Netherlands

Erfle, V., Gesellschaft für Strahlen- und Umweltschäden, Ingolstädter Landstraße 1,8042 Neuherberg, West-Germany

Erikson, Eleanor, Health Sciences Center, University of Colorado, 4200 East Ninth Avenue, Denver, CO 80262, USA

Essex, M., Dept. Microbiology, Harvard School of PubIic Health, 665 Huntington Avenue, Boston, MA 02115, USA

Evans, L., Dept. Molecular Biology, WendelI M. Stanley Hall, University of CaIifomia, Berkeley, CA 92720, USA

Feldman, M., The Weizmann Institute of Science, P.O. Box 26, Rehovot 76 100, Israel

Fleckenstein, B., Institut für Klinische Virologie, Loschgestraße 7,8520 Erlangen, West-Germany

Forbes, Penelope, Chester Beatty Research Institute, CIifton Avenue, Belmont, Sutton, Surrey SM2 5PX, U.K.

Frei IH, E., Sidney Farber Cancer Institute, 44, Binney Street, Boston, MA 02115, USA

Friis, R. R., Institut für Virologie, Justus-Liebig-Universität, Frankfurter Straße 107,6300 Gießen, West-Germany

Furusawa, M., Dept. Biology, Osaka City University, Sugimoto-Cho, Surniyoshi­Ku, Osaka 558, Japan

Gallo, R. c., National Cancer Institute, Bethesda, MD 20014, USA Gartner, Suzanne, Dept. Radiology, Cancer Biology Research Laboratory,

Stanford University Medical Center, Stanford, CA 94305, USA Georgii, A., Medizinische Hochschule Hannover, Pathologisches Institut, Karl­

Wichert-Allee 9, 3000 Hannover-Kleefeld, West-Germany Godal, T., Norsk Hydro's Institute for Cancer Research, Montebello, Oslo 3,

Norway Graf, T., Institut für Virusforschung, Im Neuenheimer Feld 280, 6900 Heidelberg

1, West-Germany Greaves, M. F., Imperial Cancer Research Fund Laboratories, P.O. Box 123,

Lincoln's Inn Fields, London WC2A 3PX, U.K. Greenberger, J. S., Dept. Radiation Therapy, Harvard Medical School, 44, Binney

Street, Boston, MA 02115, USA Hardesty, B., Dept. Chemistry, The Clayton Foundation Biochemical Institute,

University of Texas, Austin, TX 78712, USA Hausen, H. zur, Institut für Virologie im Zentrum für Hygiene, Hermann-Herder­

Straße 11, 7800 Freiburg i. Br., West-Germany Hayward, W., Rockefeller University, 1230 York Avenue, NewYork, NY 10021,

USA Hehlmann, R., Institut für Biologie, Ingolstädter Landstraße 1,8042 Neuherberg,

West-Germany Helm, K. von der, Max v. Pettenkofer-Institut, Pettenkoferstraße 9a, 8000

München 2, West-Germany Hengartner, H., Experimentelle Pathologie, Universität Zürich, Schmelzbergstra­

ße 12, 8091 Zürich, Switzerland Hölzer, D., Zentrum für Innere Medizin und Kinderheilkunde, Universität lIlm,

Steinhövelstraße 9, 7900 Ulm, West-Germany Kabisch, H., Universitäts-KinderkIinik, Abt. für Hämatologie und Onkologie,

Martinistr. 52, 2000 Hamburg 20, West-Germany Kaplan, H. S., Dept. Radiology, Cancer Biology Research Laboratory, Stanford

University Medical School, Stanford, CA 94305, USA Kettmann, R., Faculte des Sciences Agronomiques de l'Etat, Chaire de Zootech-

nie, Gembloux, Belgium Klein, Eva, Dept.Tumor Biology, KaroIinska Institutet, 104 Stockholm 60, Sweden Klein, G., Dept. Tumor Biology, KaroIinska Institutet, 104 Stockholm 60, Sweden Kirsten, W. H., Dept. Pathology, University of Chicago, 950 East 50th Street,

Chicago, IL 60637, USA Kramer, Gisela, Dept. Chernistry, The CIayton Foundation Biochernical Institute,

University of Texas, Austin, TX 78712, USA

XVI

Kurth, R., Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Spe­mannstraße 37-39, 7400 Tübingen, West-Germany

Lajtha, L. G., Christie Hospital & Holt Radium Institute, Paterson Laboratories, Manchester M20 9BX, U.K.

Lau, Barbara, Gesellschaft für Strahlen- und Umweltforschung mbH, Abteilung Experimentelle Hämatologie und Immunologie, Landwehrstraße 61, 8000 München 15, West-Germany

Levan, G., Genetiska Institutionen, Göteborgs Universitet, Stigbergsliden 14, 41463 Göteborg, Sweden

Linemeyer, D. L., National Cancer Institute, Bethesda, l'vID 20014, USA Lister, A., Dept. Medical Oncology, St. Bartholomew's Hospital, West Smithfield,

London EC1A 7BE, U.K. Löliger, H.-Ch., Institut für Kleintierzucht, Dörnbergstraße 25-27, 3100 Celle,

West-Germany Mannweiler, K., Heinrich-Pette-Institut für Experimentelle Virologie und Immu­

nologie an der Universität Hamburg, Martinistraße 52, 2000 Hamburg 20, West-Germany

Mayer, R., Sidney Farber Cancer Institute, 44, Binney Street, Boston, MA 02115, USA

McCredie, K. B., Leukemia Service, M. D. Anderson Hospital, 6723 Bertner Avenue, Houston, TX 77030, USA

McGrath, M., Dep!. Pathology, Stanford University Medical Center, Stanford, CA 94305, USA

Mercola, Karen, Dept. Medicine, University of California, Los Angeles, CA 90024, USA

Mertelsmann, R., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Messner, H., The Ontario Cancer Institute, 5000 Sherbourne Street, Toronto, Ontario M4X, 1K9, Canada

Milleck, J., Zentralinstitut für Krebsforschung, Lindenbergerweg 80,115 Berlin­Buch, GDR

Miller, D. R., Memorial Sloan-Kettering Cancer Center, 1275 YorkAvenue, New York, NY 10021, USA

Minowada, J., Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263, USA

Mitchison, N. A., Tumor Immunolgy Unit, University College London, Gower Street, London WC1E 6BT, U.K.

Mölling, Karin, Max-Planck-Institut für Molekulare Genetik, Ihnestraße 63-73, 1000 Berlin 33, West-Germany

Moloney, W. c., Peter Bent Brigham Hospital. Hematology Division, 721 Huntington Avenue, Boston, MA 02115, USA

Moore, M. A. S., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Munk, K., Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 6900 Heidelberg, West-Germany

Neth, R., Universitäts-Kinderklinik Hamburg-Eppendorf, Martinistraße 52, 2000 Hamburg 20, West-Germany

Newman, R., Imperial Cancer Research Fund Laboratories, P.O. Box 123, Lincoln's Inn Fields, London WC2A 3PX, U.K.

Nilsson, K., The Wallenberg Laboratory, Box 562, University of Uppsala, 75122 Uppsala, Sweden

Nooter, K., Radiobiological Institute, 151 Lange Kleiweg, Rijswijk, The Nether­lands

Pragnell, 1., Beaton Institute for Cancer Research, Switchback Road, Bearsden, Glasgow G61 IBD, Scotland

Preisler, H., Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263, USA

Purtilo, D. T., Dept. Pathology, University of Massachusetts Medical Center, Worcester, MA 01605, USA

Radzun, H. J., Klinikum der Christian-Albrechts-Universität Kiel, Institut für Pathologie, Hospitalstraße 42, 2300 Kiel, West-Germany

XVII

Reitz, M., National Cancer Institute, Bethesda, MD 20014, USA Riehm, H. J., Kinderklinik der Freien Universität Berlin, Heubnerweg 6, 1000

Berlin 19, West-Germany Rivera, G., St. Jude Children's Research Hospital, 332 North Lauderdale, P.O.

Box 318, Memphis, TN 38101, USA Rodt, H., Institut für Hämatologie, Abteilung Immunologie, Landwehrstraße 61,

8000 München 2, West-Germany Rohatiner, A., Dept. Medical Oncology, St. Bartholomew's Hospital, West

Smithfield, London EC1A 7BE, U.K. Rosenberg, Naomi E., Cancer Research Center, Tufts University School of

Medicine, 136 Huntington Avenue, Boston, MA 02111, USA Rowley, Janet D., Dept. Medicine, Section of Hematology/Oncology, Box 420,

950 East 59th Street, Chicago, IL 60637, USA Sallan, S. E., Sidney Farber Cancer Institute, 44, Binney Street, Boston, MA

02115, USA Scherrer, K., Institut de Recherche Biologie-Moleculaire, F-75221 Paris Cedex 5,

France Schirrmacher, V., Institut für Virusforschung, Im Neuenheimer Feld 280, 6900

Heidelberg 1, West-Germany Schreiber, H., Dept. Pathology, La Rabida Children's Hospital and Research

Center, East 65th Street at Lake Michigan, Chicago, IL 60649, USA Simon, M. M., Krebsforschungszentrum - Immunologie, Im Neuenheimer Feld

280, 6900 Heidelberg, West-Germany Stegmann, J., Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft,

Spemannstraße 37-39, 7400 Tübingen 1, West-Germany Stehelin, D., Institut Pasteur de Lilie, 15 rue Camille Guerin, B. P. 3415,

59019 Lilie, France Storb, R., Division of Oncology, Providence Hospital, 500 17th Avenue, Seattle,

WA 98122, USA Stoye, J., Friedrich-Miescher-Institut, Grenzacherstraße 487, 4002 Basel, Swit­

zerland Stuhlmann, Heidi, Heinrich-Pette-Institut für Experimentelle Virologie und

Immunologie an der Universität Hamburg, Martinistraße 52, 2000 Hamburg 20, West-Germany

Teich, Natalie M., Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London WC2A 3PX, U.K.

Teodorescu, M., Dept. Microbiology, Medical Center, University of Illinois, Chicago, IL 60612, USA

Thiel, J., Medical Center, Dept. Surgery, Duke University, Durham, NC 27710, USA

Torelli, U., Instituto di Patologia Speciale Medica e Metodologia Clinica, Via dei Pozzo, 71, 41100 Modena, Italy

Van de Woude, G., National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20014, USA

Vogt, P. K., Dept. Microbiology, USC Medical Center, 2025 Zonal Avenue, Los Angeles, CA 90034, USA

Wernet, P., Medizinische Klinik, Abteilung Innere Medizin, Otfried-Müller-Stra-ße, 7400 Tübingen, West-Germany "

Wielckens, K., Physiologisch-Chemisches Institut, Martinistraße 52, 2000 Ham­burg 20, West-Germany

Wekerle, H., Max-Planck-Institut für Immunbiologie, Stübeweg 51, 7800 Frei­burg-Zähringen, West-Germany

Witz, 1. P., Faculty of Life Science, Dept. Microbiology, Tel-Aviv University, Tel Aviv, Israel

Wolf, H., Max v. Pettenkofer-Institut, Pettenkoferstraße 9a, 8000 München 2, West-Germany

Yohn, D. S., OSU Comprehensive Cancer Center, McCampbell Hall, Columbus, OH4310, USA

Zeuthen, J., Institute for Human Genetics, Bartholin Byggningen, Aarhus University, 8000 Aarhus C, Denmark

Zintl, F., Universitäts-Kinderklinik, Kochstraße 2, 69 Jena, GDR

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Wilsede Scholarship Holders (Granted by the Deutsche Krebshilfe and the Leukemia Society of America)

Andreesen, Reinhard, Medizinische Univ.-Klinik, Hugstetter Straße 55, D-7800 Freiburg

Bamekow, Angelika, Institut für Virologie der Justus-Liebig Universität Gießen, Fachbereich Humanmedizin, Frankfurter Straße 107, D-6300 Gießen

Bergholz, Carolyn, Dept. Microbiology, Medical Sciences Building, University of Illinois, Urbana, IL 61801, USA

Berhold, Rosemarie, Univ.-Kinderpoliklinik Gießen, Feulgenstraße 12, D-6300 Gießen

Breu, Herbert, Kinderklinik der Westfälischen Wilhelms-Universität, Abteilung für Hämatologie und Onkologie, Robert-Koch-Str. 31, D-4400 Münster

Broxmeyer, HaI. E., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA

Carbonell, Felix, Abt. für klinische Physiologie, Universität Ulm, Oberer Eselsberg, D-7900 Ulm

Dölken, Gottfried, Klinikum der Albert-Ludwigs-Universität, Med. Univ.-Klinik, Hugstetter Straße 55, D-7800 Freiburg

Dube, Shyam, Dept. Epiderniology, Yale University, 60 College Street, New Haven, er 06510, USA

Freudenstein, Christa, Deutsches Krebsforschungszentrum - Institut für Virusfor­schung, Im Neuenheimer Feld 280, D-6900 Heidelberg

Gaedicke, Gerhard, Zentrum für Kinderheilkunde, Abt. Hämatologie II, Pritt­witzstr. 43, D-7900 Ulm

Hagner, Gerhard, Freie Universität Berlin, Universitätsklinikum Charlottenburg, Abt. Innere Medizin m.S. Hämatologie und On:kologie, Spandauer Damm 130, D-1000 Berlin 19

Harbers, Klaus, Heinrich-Pette-Institut für Experimentelle Viorologie und Immunologie an der Universität Hamburg, Martinistr. 52, D-2000 Hamburg 20

Hardy, William Jr., Memorial Sloan-Kettering Cancer Center, Laboratory of Veterinary Oncology, 1275 York Avenue, New York, NY 10021,. USA

Henze, Günter, Universitätskinderklinik, Heubnerweg 6, D-1 000 B~rlin 19 Heilbronn, Regine, Stadtstraße 4, D-7800 Freiburg IsseIs, Rudolf, Ludwig-Maxirnilians-Universität München, Klinikum Großhadern,

Med. Klinik III, Marchionistr. 15, D-8000 München 70 Janka, Gritta, Abt. f. Hämatologie und Onkologie, Kinderklinik der Universität

München im Dr. von Haunerschen, Kinderspital, Lindwurmstr. 4, D-8000 München •

Koeppen, Klaus-Michael, Krankenhaus Neukölln, II. Innere Abteilung, Rudower Str. 56, D-1000 Berlin 47

Konze-Thomas, Beate, Max v. Pettenkofer-Institut für Hygiene und Med. Mikrobiologie der Ludwig-Maxirnilians-Universität, Pettenkoferstr. 9a, D-8000 München 2

Meier, Claus Richard, Gesellschaft für Strahlen- und Umweltforschung mbH, Landwehrstr. 61, D-8000 München

XIX

Meyer, Peter, Eberhard-Karls-Universität, Abt. Innere Med. 11, Otfried-Müller-Str. 25, D-7400 Tübingen

Müller, Stephan, Universitätskinderklinik, Heubnerweg 6, D-I000 Berlin 19 Nerl, Christoph, Straßberger Str. 20, D-8000 München 40 Ritter, Jörg, Universitäts-Kinderklinik, Abt. für Hämatologie und Onkologie und

der Poliklinik, Robert-Koch-Str. 31, D-4400 Münster Schmitz, N., Zentrum für Innere Medizin am Klinikum der Justus-Liebig-Univer­

sität, D-6300 Gießen Seelis, Rudolfo E. A., Abt. Innere Medizin 11 der Med. Fakultät an der

Rhein-Westf.-Techn. Hochschule Aachen, Goethestr. 27-29, D-5100 Aachen Siegert, Wolfgang, Med. Klinik 111, Klinikum Großhadern, Marchionistr. 15,

D-8000 München Snyder, Harry, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue,

New York, NY 10021, USA Stünkel, Klaus, Gesellschaft für Strahlen- und Umweltforschung mbH, Land­

wehrstr. 61, D-8000 München Wernicke, Dorothee, Friedrich-Miescher-Laboratorium der Max-Planck-Gesell­

schaft, Spemannstr. 37-39, D-7400 Tübingen Weh, Hans-Josef, Akazienstr. 13, D-6501 Klein-Winternheim Ziegler, Andreas, Med. Klinik, Innere Abteilung, Eberhard-Karls-Universität,

Otfried-Müller-Str., D-7400 Tübingen

xx

Preface

Gut ist eine Lehrart, wo man vom Bekannten zum Unbekannten fortschreitet; schön ist sie, wenn sie sokratisch ist, d.i. wenn sie dieselben Wahrheiten aus dem Kopf und Herzen des Zuhörers herausfragt. Bei der ersten werden dem Verstand seine Uberzeugungen in Form abgefordert, bei der zweiten sie ihm abgelockt.

Professor Friederich Schiller Jena, in a letter written on 23 February 1793 to his friend and supporter Körner, father of the poet Theodor Körner.

Established clinicians and scientists as weil as students aga in tried the Wilsede experiment for three days and nights and learned from each other. In our fourth Wilsede meeting on "Modern Trends in Human Leukemia" we concentrated once again on questions re gar ding the practical application of research and its benefits to the patient.

The main emphasis of leukemia research has changed since the first Wilsede meeting in 1973. Virology is no longer the sole interest. Advances in immunology and cell genetics and a better understanding of

Dr. h. c. Alfred Toepfer speeking with participants of the meeting in Wilsede

XXI

Arrival and discussion of participants in front of the meeting pI ace " De Emmenhoff"

XXII

Personal and scientific discussion in Wilsede June 1980 Fotos : R. Völs

XXIII

the mechanisms regulating normal and pathological blood cell differen­tiation have had a considerable impact on the direction of leukemia research.

I would like to thank all chairmen for their efforts in organizing a program which included most of the important results and also future aspects of human leukemia. We have learned at the Wilsede meeting that the individual fields of interest raise similar cell biological questions, the solution of which require increasing efforts for multidisciplinary collabo­ration. At the next Wilsede meeting in 1982 new insights certainly will have become apparent through additional knowledge in the field of cytogenetics of cancer cells and through new possibilities in the application of monoc1onal antibodies and purification of specific regula­tory proteins with inhibitory and stimulating effects on blood cell differentiation.

During the meeting we usually stayed in De Emmenhoff in Wilsede. We would like to express our gratitude to all Wilsede people for their hospitality and also to the Verein Naturschutzpark e.V., and last but not least to Dr. Alfred Toepfer and his associates for their idealistic efforts to preserve this peaceful place for uso

Hamburg, April 1981 RolfNeth

XXIV

Acknowledgement

We should like to thank all who made this workshop possible:

Deutsche Forschungsgemeinschaft* Deutsche Krebshilfe Deutsches Krebsforschungszentrum Heidelberg Freie und Hansestadt Hamburg Hamburger Landesverband für Krebsbekämpfung und Krebsforschung e.V. Hamburgische Wissenschaftliche Stiftung Internationale Union Against Cancer (UICC)** Leukemia Society of America Paul Martini Stiftung Niedersächsisches Sozialministerium Universität Hamburg (Hertha-Grober-Stiftung)

For generous hospitality we thank the Stiftung F. V. S. zu Hamburg, the Amerikahaus in Hamburg, the Freie and Hansestadt Hamburg, the Hamburger Staatsoper and the HADAG Seetouristik und Fährdienst AG

* Contract No. 4851/10/79 ** National Cancer Institute, National Institutes of Health, Contract No.

N01-CO-65341

xxv