h Plc Detectors
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HPLC Bulk Property Detectors
Refractive Index Detector
Refractive Index Detector
Plus:1) Measures a bulk property2) Nearly Universal (different RI than mobile phase)3) Comparable response for different analytes4) Detects species with no chromophores
Minus:1) Temperature dependent2) Poor sensitivity (LOD ≈ 100 ng)3) No gradient elution
HPLC Bulk Property Detectors
Evaporative Light Scattering Detector
Solid state laser
HPLC Bulk Property Detectors
Plus:1) Measures a bulk property2) Nearly Universal (must be non-volatile)3) Does not detect liquids (gradients are ok)4) Detects species with no chromophores
Minus:1) Signal not linear with concentration2) Fair sensitivity (LOD ≈ 1 ng)3) No salts or buffers in mobile phase
Evaporative Light Scattering Detector
HPLC Bulk Property Detectors
Conductivity Detector
HPLC Bulk Property Detectors
Plus:1) Measures a bulk property2) Nearly Universal (must be ionic)3) Common for Ion Exchange LC4) Detects species with no chromophores5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) No salts or buffers in mobile phase3) Gradients a challenge
HPLC Bulk Property Detectors
Conductivity Detector
HPLC Specific Property Detectors
Conventional UV-Vis Absorption Detector
Hg PenLamp
254 nm
Photodiode 2
Photodiode 1
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
DeuteriumLamp
PhotodiodeArray
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
HPLC Specific Property Detectors
Multi-wavelength UV-Vis Absorption Detector
Plus:1) Measures a specific property2) Nearly Universal (must have chromophore)3) Most common of all detectors (~75%)4) Potential to provide qualitative info.5) Simple, robust
Minus:1) Fair sensitivity (LOD ≈ 1 ng)2) Expensive with PDA, limited with Hg Lamp3) Misses some important analytes
HPLC Specific Property Detectors
Fluorescence Detector
HPLC Specific Property Detectors
Fluorescence Detector
HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (must fluoresce)3) Second most common of all detectors (~15%)4) High sensitivity (LOD ≈ 0.01 ng)5) Can interrogate very small volumes
Minus:1) Not Universal2) Limited Applications
Fluorescence Detector
HPLC Specific Property Detectors
Electrochemical Detector
HPLC Specific Property Detectors
Plus:1) Measures a specific property2) Highly Selective (depends on reduction potential)3) High sensitivity (LOD ≈ 0.01 ng)
Minus:1) Not Universal2) Must have electrolyte in mobile phase3) Mobile phase must be aqueous4) Gradients not possible
Electrochemical Detector
HPLC Element Specific Detectors
ICP Detector Detector
Plus:1) Can be Highly Selective2) Can be nearly Universal3) Can have single sensitivity for all analytes
Minus:1) $$$2) Mobile phase must be aqueous4) Mobile phase must not contain element of interest
ICP Detector Detector
HPLC Element Specific Detectors
UV-Vis detection of sugars in juice
UV-Vis detection of sugars in juice
ICP Carbon detection of sugars in juice
ICP Carbon detection of sugars in orange juice
ICP Carbon detection of amino acids
ICP Carbon detection of amino acids
Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
Tag analytes with fluorophoreBefore or After Separation
HPLC Derivatization Detectors
Detect the change in a constant signalas the analyte passes
HPLC Indirect Detectors
Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector
Recent HPLC Detector
Hollow Cathode Detector
Recent HPLC Detector