Group leader: Mireille Ansaldi, Research Directorlcb.cnrs-mrs.fr/IMG/pdf/fiche_ansaldi-2.pdf ·...
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1 2 3 4 5 L.C.B LABORATOIRE DE CHIMIE BACTERIENNE PHAGE CYCLE AND BACTERIAL METABOLISM Group leader: Mireille Ansaldi, Research Director [email protected] 04 91 16 45 85 Research in our group focuses on several aspects of bacteriophage biology and their applications. On one hand, we aim at elucidating host-phage interactions utilizing up to date molecular and imaging approaches. We mainly focus on temperate phage infection and lysogenic conversion to understand how this shapes bacterial genome evolution and influences the host physiology using several bacterial models such as Escherichia coli, Salmonella enterica and Xylella fastidiosa. The elucidation of the regulatory interplays between prophage and host genes and the deciphering of how the host regulatory network influences prophage gene expression and prophage maintenance are central topics in our work. On the other hand, we are involved in translational projects based on the knowledge acquired on phage biology. We develop phage-based biotechnological tools such as highly specific biosensors that only detect live bacteria. We are also interested in the use of virulent phages as therapeutic tools and on their synergistic effect in combination with existing therapeutic strategies. Lysogenic infection by a temperate bacteriophage © E. Wolfson & M. Ansaldi Bacteriophages Lysogeny Host-phage interactions Genetic regulation Genome evolution Biotechnologies Phage-based tools Puvirajesinghe TM, Elantak L, Lignon S, Franche N, Ilbert M, Ansaldi M. (2012) DnaJ (Hsp40 protein) binding to folded substrate impacts KplE1 prophage excision efficiency. J Biol Chem. 287(17):14169-77. Panis G, Franche N, Méjean V, Ansaldi M. (2012) Insights into the functions of a prophage recombination directionality factor. Viruses. 4(11):2417-31. Menouni R, Champ S, Espinosa L, Boudvillain M, Ansaldi M. (2013) Transcription termination controls prophage maintenance in Escherichia coli genomes. Proc Natl Acad Sci U S A. 110(35):14414-9. Vinay M, Franche N, Grégori G, Fantino JR, Pouillot F, Ansaldi M. (2015) Phage-Based fluorescent biosensor prototypes to specifically detect enteric bacteria such as E. coli and Salmonella enterica Typhimurium. PLoS One. 10(7):e0131466. Franche N, Vinay M, Ansaldi M. (2016) Substrate-independent luminescent phage-based biosensor to specifically detect enteric bacteria such as E. coli. Environ Sci Pollut Res Int. PUBLICATIONS Laboratoire de Chimie Bactérienne - UMR 7283 31, Chemin Joseph AIGUIER 13009 MARSEILLE
Transcript of Group leader: Mireille Ansaldi, Research Directorlcb.cnrs-mrs.fr/IMG/pdf/fiche_ansaldi-2.pdf ·...
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L.C.BLABORATOIRE DE CHIMIE BACTERIENNE
PHAGE CYCLE AND BACTERIAL METABOLISM Group leader: Mireille Ansaldi, Research Director
[email protected] 04 91 16 45 85
Research in our group focuses on several aspects of bacteriophage biology and their applications. On one hand, we aim at elucidating host-phage interactions utilizing up to date molecular and imaging approaches. We mainly focus on temperate phage infection and lysogenic conversion to understand how this shapes bacterial genome evolution and influences the host physiology using several bacterial models such as Escherichia coli, Salmonella enterica and Xylella fastidiosa. The elucidation of the regulatory interplays between prophage and host genes and the deciphering of how the host regulatory network influences prophage gene expression and prophage maintenance are central topics in our work. On the other hand, we are involved in translational projects based on the knowledge acquired on phage biology. We develop phage-based biotechnological tools such as highly specific biosensors that only detect live bacteria. We are also interested in the use of virulent phages as therapeutic tools and on their synergistic effect in combination with existing therapeutic strategies.
Lysogenic infectionby a temperate bacteriophage
© E. Wolfson & M. Ansaldi BacteriophagesLysogenyHost-phage interactionsGenetic regulation
Genome evolutionBiotechnologiesPhage-based tools
Puvirajesinghe TM, Elantak L, Lignon S, Franche N, Ilbert M, Ansaldi M. (2012) DnaJ (Hsp40 protein) binding to folded substrate impacts KplE1 prophage excision efficiency. J Biol Chem. 287(17):14169-77.
Panis G, Franche N, Méjean V, Ansaldi M. (2012) Insights into the functions of a prophage recombination directionality factor. Viruses. 4(11):2417-31.
Menouni R, Champ S, Espinosa L, Boudvillain M, Ansaldi M. (2013) Transcription termination controls prophage maintenance in Escherichia coli genomes. Proc Natl Acad Sci U S A. 110(35):14414-9.
Vinay M, Franche N, Grégori G, Fantino JR, Pouillot F, Ansaldi M. (2015) Phage-Based fluorescent biosensor prototypes to specifically detect enteric bacteria such as E. coli and Salmonella enterica Typhimurium. PLoS One. 10(7):e0131466.
Franche N, Vinay M, Ansaldi M. (2016) Substrate-independent luminescent phage-based biosensor to specifically detect enteric bacteria such as E. coli. Environ Sci Pollut Res Int.
PUBLICATIONS
Laboratoire de Chimie Bactérienne - UMR 728331, Chemin Joseph AIGUIER 13009 MARSEILLE
