HEART DISSECTION LAB. Procedure 1.Obtain a dissection pan and dissecting kit.
Gross Dissection of Heart
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Evaluation of External Cardiac and GreatVessel Morphology
Dissection of Coronary Arteries
Dissection of Heart Dissection of Cardiac Conduction System
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Examineepicardium◦ Pericarditis
◦ “Soldier’s patches” “Milk spots”
◦ Petechiae
◦ Trauma
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Atrial-ventricularconcordance
Relative proportion
of right and leftventricles
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Anatomy Origins
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Anatomy◦ Right dominant
◦ Left dominant
◦ Balanced dominance
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Anatomy Origins
Dissection◦
Transversesectioning 0.3 cmapart
◦ Estimation of luminalstenosis
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Anatomy Origins Dissection
◦ Decalcification Not typically performed Dissect origin of
coronary artery alongwith button of aorta
Fix LCA and RCA with
their respectivebranches
Decalcify andsubsequently section
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Lines of Flow◦ Typically reserved for
pediatric hearts
◦ Utilize Mayo scissors
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◦ IVC R atrialappendage Assess patency of
foramen ovale
Assess tricuspid valve
◦ IVC – posterior RVadjacent to septum RV apex
◦ RV apex – anterior RVadjacent to septum Pulmonary artery Assess RV thickness ~
1cm distal to TV
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◦ Connect R pulmonaryvein and L pulmonaryvein orifices Assess mitral valve
◦ R pulmonary vein – posterior LV adjacentto septum LV apex
◦ LV apex – anterior LV– serpiginous cut to
avoid pulmonaryartery aorta Assess LV thickness ~
1cm distal to MV
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Lines of Flow◦ Typically reserved for
pediatric hearts
◦ Utilize Mayo scissors
Transversesectioning◦ Aka “breadloafing”
◦ Utilize knife
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Anterior toposterior slicesstarting at apex◦ ~ 1cm in thickness
◦ Stop ~ 1cm distal toAV valves
The atria andremaining portion
of ventricles aredissected by linesof flow
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Remove anyresidual atrial andventricular clot
Section aorta andpulmonary artery ~1cm distal to valves
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Examine for◦ Antemortem
thrombus◦ Subendocardial
fibrosis◦ Cusp/leaflet
morphology◦ Ventricular
dilatation◦ Ventricular
hypertrophy◦ Acute / remote
infarction◦ Myocarditis
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Preparation◦ Gently pack atria with
cheesecloth
◦ Fix overnight in 20%
formalin
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SA Node◦ 1st cut – Anterior to
crista terminalisthrough pectinatemuscles
◦ 2nd cut - Along upperborder of atrium intoSVC
◦ 3rd cut – SVC to IVCalong right atrial
septum◦ 4th cut – complete the
rectangle
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SA Node◦ Section along the
long axis to produce6 – 8 sections
◦ Look for sinus nodeartery
◦ Ask for trichrome
stain
Ages 10 – 90 yrs % ofcollagen expectedwithin node is ~ equalto age
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AV Node◦ Remove rectangle of
tissue containingTriangle of Koch
◦ Section along longaxis to produce 6 – 10 sections Label AV1 – AV#,
coronary sinus ventricular septum
◦ Ask for trichrome
stain
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