Grds international conference on health and life (19)
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Transcript of Grds international conference on health and life (19)
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Evaluation of wound healing activity of oral administration of ethanolic extract of Michelia champaca in diabetic wistar rats Shenoy S*, Gowda A, Shanbhag T, Prabhu K,
Nelluri V
*Department of Pharmacology, Kasturba Medical College, Manipal, India
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Background
Wound healing - impaired in patients with diabetes
mellitus. Michelia champaca - family Magnoliaceae.
Flowers used by traditional healers for
ulcers, skin disease and wounds
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Objective
To assess the effect of oral administration of ethanolic
extract of flowers of Michelia champaca on
healing of incision and excision wounds in
diabetic wistar rats.
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Methods
Approval from Institutional Animal Ethics Committee obtained
Ethanolic extract of flowers of Michelia champaca
(MC) was prepared
Suspension of the extract made by using CMC 0.5% (carboxymethylcellulose)
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Wound models
Incision wound
Excision wound
Animals
Five groups in each model
Six rats in each group
Total adult albino rats, wistar strain = 60
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Group Drug, route
Group I (non-diabetic control)
CMC 0.5%, oral
Group II (diabetic control )
CMC 0.5%, oral
Group III (diabetic test)
100 mg/kg of Michelia champaca extract, oral
Group IV (diabetic test)
200 mg/kg of Michelia champaca, extract, oral
Group V (diabetic test)
400 mg/kg of Michelia champaca , extract, oral
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Induction of diabetes
Streptozotocin (STZ) solution :
intraperitoneally - 30mg/kg
- four groups of rats
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Incision wound
Two 6 cm long paravertebral straight incisions - through
entire thickness of the skin on the back of the rats.
closed with intermittent sutures
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Parameter assessed
10th postoperative day - breaking strength of the wound –
continuous constant waterflow technique of Lee
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Excision wound model Parameters assessed
Rate of wound contraction (%) =
initial wound size – specific day wound size
initial wound sizex 100
Period of epithelization
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wound created on day 7 after induction of diabetes
anesthesia - ketamine (80 mg/kg) i.p.
round seal of 2.5 cm diameter - on the dorsal interscapular region, 5 cm away from the ears
full thickness skin from demarcated area excised
→ wound - 400 mm2 & 2 mm depth
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Duration of drug administration
from day 0 (day of wounding)
to day 21 or
till wound was completely healed,
whichever occurred earlier
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Histopathology
10th postoperative day,
granulation tissue from wound area –
using a punch biopsy needle (5 mm), under
ketamine anesthesia
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Statistical analysis
one-way ANOVA followed by
Tukey Post-hoc test
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Results
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Effect of Michelia champaca on breaking strength of incision wound
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Groups Drugs Breaking strength (g)
( Mean ± SEM)
Group ICMC 0.5%, oral
(nondiabetic control) 256.56 ± 6.54
Group IICMC 0.5%, oral(diabetic control) 222.61 ± 4.62*
Group III100 mg/kg Michelia
champaca extract, oral 238.17 ± 5.56
Group IV200 mg/kg of Michelia champaca extract, oral 245.78 ± 6.79
Group V400 mg/kg of Michelia champaca extract, oral 270.50 ± 4.40**
*p < 0.05 vs nondiabetic control ; **p < 0.05 vs diabetic control
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Effect of Michelia champaca on healing
of excision wound
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Groups Drug Period of epithelization - days ( Mean ± SEM)
Group I CMC 0.5%, oral(nondiabetic control)
18.86 ± 0.82
Group II CMC 0.5%, oral(diabetic control)
22.16 ± 0.45 *
Group III 100 mg/kg of Michelia champaca extract, oral
21.67 ± 0.22
Group IV 200 mg/kg of Michelia champaca extract, oral
20.72 ± 0.34
Group V
400 mg/kg of Michelia champaca extract, oral
19.31 ± 0.37 **
*p < 0.05 vs nondiabetic control ;
**p < 0.05 vs diabetic control
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Groups Wound contraction (%) (Mean ± SEM) / drugs Day 4 Day 8 Day 12 Day 16
I- CMC 0.5% 18.62±3.66 40.53±2.47 60.88±5.21 81.24±4.12
II-CMC 0.5% 15.62±1.72 28.26±5.27* 43.92±3.76* 64.1±4.04*
III-MC extract100 mg/kg
17.45±4.23 31.57±1.45 49.28±5.46 70.97±3.13
IV - MC extract 200 mg/kg
18.36±4.64 35.60±3.26 52.32±4.10 73.77±5.26
V- MC extract 400 mg/ kg
21.86±2.70 37.97±4.87 62.94±3.48** 82.42±3.58**
*p < 0.05 vs nondiabetic control ; **p < 0.05 vs diabetic control
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(c)
(e)
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Discussion
Diabetic wounds – reduced
cellular infiltration, formation of granulation tissue,
collagen synthesis, breaking strength
Michelia champaca –
promoted better organization of collagen, reepithelization,
increased wound contraction rate, breaking strength
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Michelia champaca – has
tannins, triterpenoids
antioxidant properties
antimicrobial actions
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Conclusion
Ethanolic extract of Michelia champaca on oral
administration has a beneficial influence on wound
healing in diabetic rats
.
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