Graficas
4
Tubo V1 Absorvancia 1 0.00 0.000 2 0.02 0.175 3 0.04 0.336 4 0.06 0.411 5 0.10 0.519 6 0.50 0.658 7 1.00 0.622 [S] V 0.00E+00 0.00 2.14E-05 0.12 4.29E-05 0.23 6.43E-05 0.28 1.07E-04 0.35 5.36E-04 0.45 1.07E-03 0.42 Michaelis Menten 0.00E+00 2.14E-05 4.29E-05 6.43E-05 1.07E-0 0.00 0.05 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.00 0.12 0.23 0.28 0.35 f(x) = 0.0732214285714286 x − 0.0285697142857143 R² = 0.943876122558664 Michaelis Menten [S] V
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Grafica en Bioquimica Actividad enzimatica
Transcript of Graficas
Hoja1TuboV1Absorvancia10.000.00020.020.17530.040.33640.060.41150.100.51960.500.65871.000.622
Michaelis Menten[S]V0.00E+000.002.14E-050.124.29E-050.236.43E-050.281.07E-040.355.36E-040.451.07E-030.42
Lineweaver Burk1/[S]1/V
4.667E+048.402.333E+044.381.556E+043.589.333E+032.831.867E+032.239.333E+022.36
Y=0.0001x + 1.7938 1/Vo = (Km/Vmax) * (1/[S]) + 1/Vmaxx= 1/[S]1/Vmax1.7938Y= 1/Vo1/Vmax0.5574757498Cuando 1/Vo = 01/[S] = -1/Km -1/Km = -179380.0000557476x=-1.7938/0.0001-17938
