Goal: Develop bacterial biocatalysts for consolidated bioprocessing (CBP) of lignocellulosis
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Transcript of Goal: Develop bacterial biocatalysts for consolidated bioprocessing (CBP) of lignocellulosis
Engineering Bacillus subtilis biocatalysts for production of biofuels and chemical feedstocks and biochemicals for
pharmaceutical and nutraceutical applications. Mun Su Rhee, Lusha Wei, James F. Preston
Department of Microbiology and Cell Science, University of Florida
Goal: Develop bacterial biocatalysts for consolidated bioprocessing (CBP) of lignocellulosisa. Secretion of cellulases and xylanases to process cellulose and xylansb. Fermentation of glucose and xylose for production of biofuels and chemicals
Objective: Engineer Bacillus subtilis for CBP of methylglucurono(arabino)xylans (MeG(A)Xn)c. Engineer B. subtilis to convert MeGAXn to lactate for production of bioplasticsd. Engineer B. subtilis to convert MeGXn to xylooligosaccharides : acidic (U-XOS) for
production of anti-inflamatory agents and neutral (XOS) for production of prebiotics
Collaborators: L. O. Ingram, K. T. Shanmugam, W. Vermerris, J. Erickson,
Support: FESC, USDA Biomass Research & Development Initiative
Cellulose fiber32-48%
MeGAXn 18-35% MeGXn
Interaction of Major Polymeric Sugars in Lignocellulosic Biomass
Cellulose fibers form through hydrogen bonding interactions between individual cellulose stands
The noncarbohydrate polymer lignin embeds the interacting cellulose and glucuronoxylan through ester linkages to glucuronoxylan
Monocots: grasses, e.g. sorghum, sugarcane
Dicots: hardwoods, e.g.eucalyptus, sweetgum
Methylglucuronoarabinoxylan, MeGAXn
Methylglucuronoxylan, MeGXn
Pretreatment of O-acetyl-glucuronoarabinoxylan and enzymatic depolymerization of 4-O-methylglucuronoxylan
Schematic for bioconversion of MeGXn to U-XOS by B. subtilis strains. MeGX3, MeGX4 or MeGX4-18 accumulate in the cultures of B.s. 168,
MR42(ΔxynC) or MR44(ΔxynA), allowing production of XOS and/or U-XOS
MeGXn
(Sweetgum xylan)
MeGX4-12 MeGX4
MeGX3
XOSX2, X3
XynC (GH30) XynA (GH11)
XynA (GH11)
MR44(168, ΔxynA )
MeGX3
XynC (GH30)
MR42(168, ΔxynC )
168
X2,X3XOS
Hemicellulose, xylan
Xylanase GH11Xylanase GH30
GH11 GH30
Acidic XOS
XOSPrebiotics
PharmaceuticalsNutraceuticals
Xylanase GH10Xylanase GH67
XOS
GH10 GH67
Fermentation
Biofuel and Biochemicals
Engineering B. subtilis as Biocatalyst for Production of Biofuel and Value-added Products
Bacillus subtilis
Bacillus subtilis
Monocot : MeGAXnDicot : MeGXn
A, XOS