GHS Science CAPT Embedded Task Review
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Transcript of GHS Science CAPT Embedded Task Review
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GHS ScienceCAPT Embedded Task Review
Lab Investigation: Enzymes
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Your Task
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Lab Design
1. Place filter paper in a funnel and put funnel in a flask.
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Funnel with filter paper in it
Flask
Most GHS students used a procedure that looked something like this:
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Lab Design
2. Set up three flasks with funnels.
5 A B C
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Lab Design
3. Pour exactly 20 g of apple sauce into each funnel.
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20 g of apple sauce
20 g of apple sauce
20 g of apple sauce
A B C
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Lab Design
4. Add a different enzyme to each funnel.
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8 ml of Pectinase
8 ml of Cellulase
No enzyme added
A B C
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Lab Design
5. Let each flask sit for exactly 10 minutes.
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Lab Design
6. After 10 minutes, measure how much juice was produced from each flask using a graduated cylinder.
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Graduated Cylinder
A B C
Apple Juice Produced
Apple Juice Produced
Apple Juice Produced
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Sample DataEnzyme(s)
Flask APectinase
Flask BCellulase
Flask CNo Enzyme
AddedAmount of
Juice Produced3.0 ml 5.0 ml 0.5 ml
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Analysis Questions
Directions: On a separate sheet of paper, answer the following questions in complete sentences.
1. Identify the following for this experiment:
a. Problem statementb. Independent variablec. Dependent variable
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Analysis Questions
2. Identify two variables that were held constant in this
experiment.
3. Explain why it is important to hold these variables constant.
4. What was the substrate and the product(s) in this experiment?
5. Why was it important that “flask C” was used?
6. Based on the data, what would your conclusion be for this
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Going Further… Challenge Questions, Set 1
7. After performing this experiment, you learn from the apple juice
company that Pectinase and Cellulase cost different amounts:
a. Pectinase: $.50 per ml
b. Cellulase: $1.00 per ml
The apple juice company wants you to be as cost-effective as
possible in producing the most amount of juice.
Explain to the company which enzyme is most cost-effective (ml/$)?
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Going Further… Challenge Questions, Set 2
A group of students are convinced that pectinase is the most cost-
effective enzyme to choose to produce apple juice for the company.
They think that temperature may affect how much juice pectinase can
produce.
In a new experiment, they test their hypothesis. Using the same basic
design as the original experiment, the students heat apple sauce with
pectinase to different temperatures to see which temperature is
optimal for producing the most apple juice.
The temperatures are: 0°C, 20°C, 40°C, 60°C, 80°C16
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Going Further… Challenge Questions, Set 2
The results of the students’ new experiment are shown below:
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0 20 40 60 80Degrees Celcius
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Going Further… Challenge Questions, Set 2
8. Explain the behavior of pectinase in temperature range 0-20 °C.
9. Explain the behavior of pectinase in temperature range 50-80 °C.
10. What would be an appropriate conclusion for the new experiment?
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