Genomics: Fish DNA Barcoding & Protein Profiling Workshop

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In electrophoresis, molecules such as DNA and proteins on top of an electric field migrate down toward the opposite charged electrode and separate according to their charge and shape. This technique revealsthe number of proteins found in each sample, their size and the similarities or differences between them. Electrophoresis can be done byDNA barcoding, based on using a DNA strand to facilitate species identification,in which various DNA are isolated into a lysis solution. Then, the primer mix is prepared. Later, after setting up the PCR reactions, DNA are amplified in the thermal cycler, which are then loaded into the agarose gel solutions. Electrophoresis can also be done by protein fingerprinting, whichidentifies proteins and evolutionary relationships among fish, in which proteinsare placed in microtubes containing a buffer. The samples are flicked, incubated andheated to denature the proteins before beingloaded into the polyacrylamide gel solutions. After these techniques were presented in the lab, an expert in invertebrate studies displayed his research on analyzing the taxonomy and genetic patterns of Nemertia for being able to build a genealogical tree.

Transcript of Genomics: Fish DNA Barcoding & Protein Profiling Workshop

Page 1: Genomics: Fish DNA Barcoding & Protein Profiling Workshop

In electrophoresis, molecules such as DNA and proteins on top of an electric field migrate down toward

the opposite charged electrode and separate according to their charge and shape. This technique

revealsthe number of proteins found in each sample, their size and the similarities or differences

between them. Electrophoresis can be done byDNA barcoding, based on using a DNA strand to facilitate

species identification,in which various DNA are isolated into a lysis solution. Then, the primer mix is

prepared. Later, after setting up the PCR reactions, DNA are amplified in the thermal cycler, which are

then loaded into the agarose gel solutions. Electrophoresis can also be done by protein fingerprinting,

whichidentifies proteins and evolutionary relationships among fish, in which proteinsare placed in

microtubes containing a buffer. The samples are flicked, incubated andheated to denature the proteins

before beingloaded into the polyacrylamide gel solutions. After these techniques were presented in the

lab, an expert in invertebrate studies displayed his research on analyzing the taxonomy and genetic

patterns of Nemertia for being able to build a genealogical tree.