Genomics and Epigenomics Shared Resource (GESR)Habtom Ressom, PhDDirector

Catalin Marian, MD, PhDDirector

David Goerlitz, MSManager

Xiaojun Zou, MSResearch Associate

Md Islam, MDResearch Associate

Xin (James) Li, MSBioinformatician

GESR New Service

Quantitative DNA Methylation AnalysisQuantitative DNA Methylation Analysis

by Pyrosequencingby Pyrosequencing• CpG methylation analysis at gene promoter levels• CpG methylation of LINE elements to estimate global

genomic DNA methylation

For more details visit http://lombardi.georgetown.edu/research/sharedresources/gesr

GESR New Service

Custom Low- to Mid-Plex SNP Genotyping• The Illumina GoldenGate Genotyping Assay allows you to

create your own custom panels to interrogate 48-, 96-, 144-, 192-, and 384-plex SNP loci/sample in 96 samples simultaneously.

• This assay utilizes the Illumina BeadXpress platform.

For more details visit http://lombardi.georgetown.edu/research/sharedresources/gesr/

GESR New Service

MicroarraysMicroarrays

SNP and CNV Analysis:•New Affymetrix Genome-Wide Human SNP Array 6.0 features 1.8 million genetic markers

•Includes more than 906,600 single nucleotide polymorphisms (SNPs) and more than 946,000 probes for the detection of copy number variation.

For more details visit http://lombardi.georgetown.edu/research/sharedresources/gesr/

GESR New ServiceMicroarraysMicroarrays

Whole Transcriptome Expression Analysis:

•Each of 28,869 genes on the GeneChip® Human Gene 1.0 ST Array is represented on the array by approximately 26 probes spread across the full length of the gene

•Provides more complete and more accurate picture of gene expression than 3’ based expression array designs.

For more details visit http://lombardi.georgetown.edu/research/sharedresources/gesr/

GESR New ServicesMICRO RNA PROFILING

•miRNA analysis using the Applied Biosystems TaqMan® Human MicroRNA Array Set.

•Set enables accurate measurement of all mature human miRNAs in various biospecimens, including FFPE tissue and liquid samples

For more details visit http://lombardi.georgetown.edu/research/sharedresources/gesr/

Contact Information:Contact Information:Directors: Habtom Ressom, PhD, Telephone: (202) 687-2283,

hwr@georgetown.eduCatalin Marian, MD, PhD, Telephone: (202) 687-4438,

cm444@georgetown.edu

Manager: David Goerlitz, MS, Telephone: (202) 687-0832,

dsg3@georgetown.edu

Research Associates: Xiaojun Zou, MS, Telephone: (202) 687-7776, zoux@georgetown.edu Md Islam, MD, Telephone: (202) 687-7776, mni3@georgetown.edu

Bioinformatician: Xin (James) Li, MS, Telephone: (202) 687-8533,

xl35@georgetown.edu

Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01

  In your publications, remember to mention support provided from the GHUCCTS.