Mechanism of the genetic instability of the TRS during replication.
Genome instability in human oocytes and embryoscme-utilities.com/mailshotcme/Material for...
Transcript of Genome instability in human oocytes and embryoscme-utilities.com/mailshotcme/Material for...
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Eva R Hoffmann
DNRF Center for Chromosome Stability
University of Copenhagen
Denmark
Basic science session:
Genome instability in human oocytes and
embryos
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Disclosure
- Funding and collaboration: Illumina Inc., Cooper Genomics, iGenomix
- Funding: Novo Nordisk Foundation, ERC, ReproUnion (Ferring)
- Research conducted under ethics license: H-16044731
- Data protection license (GDPR): SUND-2016-50
- Declare no competing interests
28355183
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Genetic diversity and genome instability
• > 7,000 genetic disorders
• 50-75% with impact on pediatric health
• 6% of children have a genetic predisposition to ‘serious’ disease
• Majority are rare (< 1:2,000)
(Wright et al., Nat Gen 2017)
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Technology-driven discovery
>7,000 genomic
disorders
(Blanshard et al., Methods in Mol. Biol.,2018)
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Germline: origins of genome instability
cleavage stage blastocystmature
MII
zygote births
Tissue differentiationEmbryonic mitosisMeiosis (& PGC)
“black box”
Birth
fetus
sperm
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Hypothesis: understand the basics and translate
(Butler, Nature, 2008)
“Valley of Death”
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Events in pregnancy losses and live births
Major of aneuploidy in pregnancy losses and live births
are whole chromosomes from meiosis
(Hassold and Hunt, Nat. Rev. Genet. 2001)
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Natural fertility in human is ‘inefficent’
Euploid
eggs
Human
fertility
(Gruhn, J.R.*, Zielinska, A.P.*, Shukla, V.*, Blanchard, R.*, ... Yding Andersen, Schuh, M., and E.R. Hoffmann, Science , 2019)
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Whole chromosome aneuploidy shapes natural fertility
Euploid
eggs
Human
fertility
(Gruhn, J.R.*, Zielinska, A.P.*, Shukla, V.*, Blanchard, R.*, ... Yding Andersen, Schuh, M., and E.R. Hoffmann, Science , 2019)
Meiotic errors in oocytes follow a U shape
Error types differ w. age
Chromosomes afflicted differ
Cohesion loss drives aneuploidy in AMAs
Very young females?
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Idea is that meiotic errors result in uniform, aneuploid embryo
Selection (PGT-A: noninvasive)
Improve clinical endpoints
(live births, PL, reduce twin pregnancies)
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Heritability of in- and subfertility?
Common phenotype: common variants, each with small effect
Rare, pathogenic mutations, with high penetrance (explain very
few cases- e.g. TUBB8)
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Genetic regulation of aneuploidy in human eggs?
Jonathan M. Chernus1, Emily G. Allen2, Zhen Zeng3, Eva R. Hoffman4, Terry J. Hassold5,
Eleanor Feingold1,3*¶, Stephanie L. Sherman2
“A candidate gene analysis and GWAS for genes associated with maternal
nondisjunction of chromosome 21”
(PLoS Genet., in press)
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Genetic regulation of aneuploidy in human eggs?
Jonathan M. Chernus1, Emily G. Allen2, Zhen Zeng3, Eva R. Hoffman4, Terry J. Hassold5,
Eleanor Feingold1,3*¶, Stephanie L. Sherman2
Translational perspective: Polygenic risk scores?
(PLoS Genet., in press)
“A candidate gene analysis and GWAS for genes associated with maternal
nondisjunction of chromosome 21”
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Current model for maternal meiotic errors in AMA
(Capablo, Hoffmann et al, HRU, 2017)
Ottolini et al., Nat Genet, 2015)
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Capa
Maternal age
‘cohesion weakning/loss’, ‘epigenetic drift’
Recombination counteracts cohesion loss (with age)
(Capablo, Hoffmann et al, HRU, 2017)
Ottolini et al., Nat Genet, 2015)
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Capa
Maternal age
‘cohesion weakning/loss’, ‘epigenetic drift’
Recombination counteracts cohesion loss (with age)
> 200 genes
> 80 genes
(Capablo, Hoffmann et al, HRU, 2017)
Ottolini et al., Nat Genet, 2015)
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Clinical utility?
(Gruhn, J.R.*, Zielinska, A.P.*, Shukla, V.*, Blanchard, R.*, ... Yding Andersen, Schuh, M., and E.R. Hoffmann, Science , 2019)
Personalized risk?
Aging features?
(surrogate, biomarker)
Genetics?
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Mosaicism
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Mosaicism and segmental variations
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Technological limitations and challenges
• Biopsy
• Technology (FISH, WGA, SNP, NGS)
• Validation (problematic for ‘true mosaics’)
• Algorithms
• Resolution (segmentals) (Fragouli et al., HRU, 2019)
(Capalbo and Rienzi, Fert Ster, 2017)
(Weissmann et al., Fert Ster, 2017)
(Harton, et al., Fert Ster, 2017)
(Vera-Rodriguez and Rubio, Fert Ster, 2017)
(Munne and Wells, Fert Ster, 2017)
(Marin, et al., Curr Opin Obstet Gynecol, 2017)
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Origins of mosaic embryos
cleavage stage blastocystmature
MII
zygote births
Tissue differentiationEmbryonic mitosisMeiosis (& PGC)
“black box”
Birth
fetus
sperm
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cleavage stage blastocystmature
MII
zygote births
Tissue differentiationEmbryonic mitosisMeiosis (& PGC)
“black box”
Birth
fetus
70-90% 5-25% 1-2% 0.5%
(Vanneste et al., Nat Med, 2009)
(van Echten-Arends, HRU, 2011)
(Fragouli et al., HRU, 2019)
(Capalbo and Rienzi, Fert Ster, 2017)
(Weissmann et al., Fert Ster, 2017)
(Harton, et al., Fert Ster, 2017)
(Vera-Rodriguez and Rubio, Fert Ster, 2017)
(Munne and Wells, Fert Ster, 2017)
(Marin, et al., Curr Opin Obstet Gynecol, 2017)
(Levy et al., Obst Gyn, 2014)
Origins of mosaic embryos
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Origins and fates of mosaic embryos
cleavage stage blastocystmature
MII
zygote births
DifferentiationEmbryonic mitosisMeiosis (& PGC)
“black box”
Birth
fetus
70-90% 5-25% 1-2% 0.5%
Maternal to zygotic transition (embryonic genome activation)
30-40% of embryos arrest
(markers of genome instability: yH2AX, micronuclei,DNA bridges,
fragmented cells)
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Unstable maternal to zygotic transition
(McCoy et al., Science, 2015)
(McCoy et al., HMG, 2018)
(Zhang et al., Fert Ste, 2017)rs2305957
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Unstable maternal-zygotic transition: tripolar spindles?
rs2305957
(McCoy et al., Science, 2015)
(McCoy et al., PloS Genet, 2015)
(McCoy et al., HMG, 2018)
(Zhang et al., Fert Ste, 2017)
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(McCoy et al., Science, 2015)
(McCoy et al., PloS Genet, 2015)
(McCoy et al., HMG, 2018)
(Zhang et al., Fert Ste, 2017)
Unstable maternal-zygotic transition: tripolar spindles?
rs2305957
• ‘Chaotic karyotype’
• Day 3 embryos
• Arrest
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Mechanism leading to tripolar spindles
…and when the two spindles
fail to merge, embryos with
‘tripolar’-like spindles and
multinucleated cells
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Conservation in human embryos?
• Human embryos maintain separate pronuclei after fertilization
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Conservation in human embryos?
• Spacial separation of parental genomes in human zygotes
(van der Werken et al., Nat. Comm., 2014)
• Human embryos maintain separate pronuclei after fertilization
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Conservation in human embryos?
• Spacial separation of parental genomes in human zygotes
(van der Werken et al., Nat. Comm., 2014)
• Human embryos maintain separate pronuclei after fertilization
• Failure to align spindles in mouse produces clinical phenotypes in human
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Conservation in human embryos?
• Spacial separation of parental genomes in human zygotes
(van der Werken et al., Nat. Comm., 2014)
• ‘Tripolar spindles’ have been observed in human (Santhananthan et al., PNAS, 1991; Chatzimeletiou et al., Hum Repro. 2005)
• Human embryos maintain separate pronuclei after fertilization
• Failure to align spindles in mouse produces clinical phenotypes in human
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Conservation in human embryos?
• Spacial separation of parental genomes in human zygotes
(van der Werken et al., Nat. Comm., 2014)
• ‘Tripolar spindles’ have been observed in human (Santhananthan et al., PNAS, 1991; Chatzimeletiou et al., Hum Repro. 2005)
• Infer that chaotic, ‘tripolar’ segregation events (McCoy et al., Hum Mol Genet. 2018)
• Human embryos maintain separate pronuclei after fertilization
• Failure to align spindles in mouse produces clinical phenotypes in human
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Conservation in human embryos?
• Spacial separation of parental genomes in human zygotes
(van der Werken et al., Nat. Comm., 2014)
• ‘Tripolar spindles’ have been observed in human (Santhananthan et al., PNAS, 1991; Chatzimeletiou et al., Hum Repro. 2005)
• Infer that chaotic, ‘tripolar’ segregation events (McCoy et al., Hum Mol Genet. 2018)
• Chaotic karyotypes result in cellular attrition and embryo arrest (Ottolini et al.,2017)
• Human embryos maintain separate pronuclei after fertilization
• Failure to align spindles in mouse produces clinical phenotypes in human
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Basic research provides mechanistic insight
What are the mechanisms- and how many patients do they account for?
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….towards personalized medicine?
One gene, one
therapy
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Acknowledgements
MPI- Göttingen
Melina Schuh
Agata Zielinska
University Hospitals, Denmark
Marie Louise Grøndahl
Claus Yding Andersen
Stine Gry Kristensen
Erik Ernst
Anne-Mette Bjørn
Lotte Colmorn
Vitrolife
Robert Blanshard
Alan Handyside
INVICTA, PL
Krzysztof Łukaszuk
Joanna Liss
Marta Krapchev
GENERA, IT
Danilo Cimadomo
Laura Rienzi
Filippo Ubaldi
Catello Scarica
iGENOMIX
Antonio Capalbo
Warwick University, UK
Geraldine Hartshorne
Deborah Taylor
Sussex University, UK
Louise Newnham
Johns Hopkins, US
Rajiv McCoy
Eva
Amruta
Ivan
Becca
Kristina
Rehannah
Marie Louise
AndyVallari
Jazib
Ajuna
SylviaDitteJenny
Bobby
Acknowledgements: All the girls and women who participated
CooperSurgical
Tony Gordon
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Acknowledgements
MPI- Göttingen
Melina Schuh
Agata Zielinska
University Hospitals, Denmark
Marie Louise Grøndahl
Claus Yding Andersen
Stine Gry Kristensen
Erik Ernst
Anne-Mette Bjørn
Lotte Colmorn
Vitrolife
Robert Blanshard
Alan Handyside
INVICTA, PL
Krzysztof Łukaszuk
Joanna Liss
Marta Krapchev
GENERA, IT
Danilo Cimadomo
Laura Rienzi
Filippo Ubaldi
Catello Scarica
iGENOMIX
Antonio Capalbo
Warwick University, UK
Geraldine Hartshorne
Deborah Taylor
Sussex University, UK
Louise Newnham
Johns Hopkins, US
Rajiv McCoy
Eva
Amruta
Ivan
Becca
Kristina
Rehannah
Marie Louise
AndyVallari
Jazib
Ajuna
SylviaDitteJenny
Bobby
Acknowledgements: All the girls and women who participated
CooperSurgical
Tony Gordon