GenikonGenikonThe New 3D Generation of The New 3D Generation of in Situ Fluorescence Hybridisationin Situ Fluorescence Hybridisation

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DapiFitc

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The evolution of the microscopes during last years ad their Automation (z axe control, control of filter set ) gave a big contribution to the

precision of the acquisition of images in FISH technique. The fusion of Nikon microscopes with Genikon “Digital System” gives today the ideal

solution to acquire and to process FISH images in a very quick and simple way. In particular, the automatic control of the times acquisition

of the cameras, gives possible a completely automatic solution.

The new digital head of the microscope 90i/80i gives the

opportunity to be controlled from software in the moving of the

single filter in a fast a very easy way. This opportunity eliminates shifting and bleaching problems.

The software offers list of capturing saved and the complete

control of the shutter of the fluorescence in order to illuminate as less as possible the sample.

With just a click of the mouse the user starts the acquisition process of all the fluorochromes of the list and gives to the system the complete control of the

exposition times in order to obtain at the end a perfect and focussed image.

The Motorization of the 90i Nikon Microscope

Nikon Software interface for the control of the microscope

Epi_fluorescence turret motorization

Fish Analysis on lymphocyte, chromosome 4 (rearranged photo b (Dr.Antonio Novelli, Roma)

Building of the fluorochromes list by the set up of the number of planes of focus

Microscope’s filters control by Genikon interface

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The new tool of automatic focus control, identifies the best contrast for every plane for each fluorochrome independently

from the one fixed at the ocular

Senza

Because in a single plane of fluorescence could happen that one signal doesn’t show the same focus

of the other, the new tool of 3D reconstruction of Genikon became very important. This function will

create one single image which will show all the single signals (for the same fluorochrome) at the same

focus plane.

Single plane image

Fusion Image of every focus planes

Thanks to the new techniques of 3 D

reconstruction and of focus control, it’s

possible to execute a building of Karyotyping

also on Fish samples

The DAPI reverse function it’s showing the probe and helps the user to recognise chromosomes

“With Autofocus tool”

Autofocus control Tool

Set up of Acquisition sequence on every plane of focus

Funzione “Dapi reverse”

Karyotyping on Fish images

HER2/Neu Analysis

Genikon with the 3D for automatic method of counting spot, offers a new procedure fast and precise to obtain the value of the ratio of spots on single nuclei or many nuclei

identified from the user on HER2NEU samples.

“Without Autofocus tool”