Genetics News
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Transcript of Genetics News
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Genetics NewsEditBase
Mystery Sequence
EditBase format
Pure text format
Problem Set 4
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Study Question 3
What is the minimum number of bases to specify the number of different amino acids found in protein?
G A C T
1 base fixed length nonoverlapping
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Study Question 3
What is the minimum number of bases to specify the number of different amino acids found in protein?
2 base, fixed length, nonoverlapping
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Study Question 3What is the minimum number of bases to specify the
number of different amino acids found in protein?
3 base, fixed length, nonoverlapping
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Growth of E. coli on a plate
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Infection by phage T4
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Infection by phage T4
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Infection by phage T4
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Growth of E. coli B + phage T4
Seed plate withE.coli B and
phage T4(not visible)
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Growth of E. coli B + phage T4
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Growth of E. coli B + phage T4
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Growth of E. coli B + phage T4
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Growth of E. coli B + phage T4
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Growth of E. coli B + phage T4
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rII region of phage T4
rII rII
T4 rII+ T4 rII -
T4 DNA T4 DNA
rIIA rIIB rIIA- rIIB
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Distinguishing T4 rII+ from rII-
T4 rII+
T4 rII -E. coli B x T4 rII+
T4 rII -E. coli K() x
T4 rII - cannot grow on E. coli K()
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Why is T4rII- defective?
T4 rII -
T4 rII+
E. coli K()
E. coli K()
No infection
Lysis
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Crick et al frameshift experiment
rIIA-
rIIA-
rIIA-
rIIA+
proflavin
proflavin
proflavin
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How to explain frameshift results?Role of proflavin in experiment
CACTCGGATA
CACTCG??GATA
GTGAGC
GTGAGCAC
Proflavin causes one base insertions
(or deletions)
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How to explain frameshift results?Wild type rIIA
rIIA+
… THE FAT CAT ATE THE BIG RAT...
Wld type rIIA
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How to explain frameshift results?Single frameshift
rIIA-
THE FAT CAR TAT ETH EBI GRA …
… THE FAT CAT ATE THE BIG RAT...
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How to explain frameshift results?Double frameshift
rIIA-
… THE FAT CAR TAT ETH EBI GRA …
… THE FAT CAT ATE THE BIG RAT...
… THE FAT CAR TAR TET HEB IGR …
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How to explain frameshift results?Triple frameshift
rIIA-
… THE FAT CAR TAT ETH EBI GRA …
… THE FAT CAT ATE THE BIG RAT...
… THE FAT CAR TAR TET HEB IGR …
… THE FAT CAR TAR TOE THE BIG ...
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Study Question 7
When will proflavin-induced mutation suppress?
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Study Question 8
Results of frameshift experiment if variable length code?
This is a variable length code
This is a varitable length code
Variable length code with GATC?