Genetic polymorphism at nine STR loci in two ethnic groups of China

Announcement of population data

Genetic polymorphism at nine STR loci in two

ethnic groups of China

Teng Chen, Shengbin Li*

Department of Forensic Science, Medical College of Xi’an Jiaotong University, Xi’an 710061, PR China

Received 24 September 2003; received in revised form 2 February 2004; accepted 5 April 2004

Available online 17 June 2004

Abstract

Genotype polymorphism studies at nine short tandem repeat (STR) loci (D3S1358, FGA, TH01, D7S820, VWA, CSF1PO,

D5S818, D13S317, TPOX) were carried out in two Ethnic Groups (Tu, Sala) living in Qinhai, a North–West province of China.

All loci were in accordance with Hardy–Weinberg equilibrium (P ¼ 0:05).

# 2004 Elsevier Ireland Ltd. All rights reserved.

Keywords: Forensic science; Short tandem repeats (STR); Population genetics; China

Population: The Tu (n ¼ 120) and Sala (n ¼ 125) Ethnic

Group selected for present study living in Qinhai belong to

North–West China. Blood samples were collected from

unrelated healthy individuals who self-declared their ethnic

origin over four generations.

Extraction: Genomic DNA was extracted by the salting

out method and quantified with electrophoresis.

PCR: Amplification was performed using 5 ng genomic

DNA of template according to the manufacture’s protocol

[1] (AmpF‘STR1 ProfilerTM PCR Amplification kit,

Applied Biosystems, Foster City, USA).

Typing: The amplified DNA products were separated

and detected using an ABI PrismTM377 DNA sequencer

(Applied Biosystems). The resultant data analysis and allele

designation were carried out using the GeneScanTM and

GenotypeTM softwares.

Analysis of date: To check genetic stability of Tu and

Sala Ethnic Groups, the allele frequencies data were ana-

lyzed based upon Hardy–Weinberg probability test. The

discrimination power (DP), paternity of exclusion (CE)

and the heterozygosity (H) were calculated [2,3].

Results: The DP, CE, H, and the polymorphism informa-

tion content (PIC) for the nine STR loci in Tu and Sala ethnic

groups from China are given in Tables 1 and 2, respectively.

Access to date: The complete data are available to any

interested reader upon request made to Shengbin Li, MD,

Forensic Science International 146 (2004) 53–55

Table 1

Allele frequency for nine STR loci in Tu population of China (n ¼ 120)

Allele D3S1358 VWA TH01 FGA TPOX CSF1PO D5S818 D7S820 D13S317

5 – – 0.0096 – – – – – –

6 – – 0.0913 – 0.0048 – – – –

7 – – 0.2548 – 0.0192 0.0048 0.0096 0.0096 0.0048

8 – – 0.0913 – 0.4952 – 0.0096 0.1827 0.2260

9 – – 0.4663 – 0.1394 0.0240 0.0673 0.0865 0.1106

9.3 – – 0.0625 – – – – – –

10 – – 0.0240 – 0.0192 0.2019 0.2308 0.1250 0.1442

* Corresponding author. Tel.: þ86-29-82656244; fax: þ86-29-82656244.

E-mail address: [email protected] (S. Li).

0379-0738/$ – see front matter # 2004 Elsevier Ireland Ltd. All rights reserved.

doi:10.1016/j.forsciint.2004.04.004

Table 1 (Continued )


11 – – – – 0.2981 0.2644 0.3462 0.3029 0.2452

12 – – – – 0.0240 0.4471 0.1923 0.2404 0.1971

13 – 0.0048 – – – 0.0529 0.1346 0.0481 0.0577

14 0.0481 0.2115 – – – 0.0048 0.0048 0.0048 0.0096

15 0.3510 0.0288 – – – – 0.0048 – 0.0048

15.2 0.0048 – – – – – – – –

16 0.3606 0.1923 – – – – – – –

17 0.1490 0.2837 – – – – – – –

18 0.0817 0.1731 – 0.0529 – – – – –

19 0.0048 0.0913 – 0.0817 – – – – –

20 – 0.0096 – 0.0577 – – – – –

20.2 – – – 0.0048 – – – – –

21 – – – 0.0913 – – – – –

21.2 – – – 0.0048 – – – – –

22 – – – 0.1587 – – – – –

22.2 – – – 0.0048 – – – – –

23 – – – 0.2308 – – – – –

23.2 – – – 0.0240 – – – – –

24 – – – 0.1202 – – – – –

25 – – – 0.0962 – – – – –

25.2 – – – 0.0096 – – – – –

26 – – – 0.0481 – – – – –

27 – – – 0.0048 – – – – –

28 – – – 0.0048 – – – – –

29 – – – 0.0048 – – – – –

H 0.7156 0.7985 0.6963 0.8736 0.6452 0.6860 0.7671 0.7916 0.8135

PD 0.8560 0.9257 0.8632 0.9691 0.7964 0.8504 0.9084 0.9197 0.9289

CE 0.5722 0.7410 0.5856 0.8838 0.4610 0.5015 0.6480 0.7219 0.7417

PIC 0.7089 0.7871 0.6888 0.8735 0.6353 0.6569 0.7436 0.7771 0.8043

H: observed heterozygosity; DP: discrimination power; CE: paternity of exclusion; PIC: polymorphism information content.

Table 2

Allele frequency for nine STR loci in Sala population of China (n ¼ 125)


6 – – 0.0847 – – – – – –

7 – – 0.2661 – 0.0040 – 0.0121 – 0.0040

8 – – 0.0323 – 0.5081 – 0.0040 0.1895 0.2460

9 – – 0.5081 – 0.1210 0.0323 0.0605 0.0645 0.1008

9.3 – – 0.0968 – – – – – –

10 – – 0.0121 – 0.0605 0.2782 0.2298 0.2097 0.1290

11 – – – – 0.2823 0.2581 0.3750 0.3065 0.2460

12 – – – – 0.0202 0.3831 0.1976 0.1694 0.1734

13 0.0040 – – – 0.0040 0.0323 0.1210 0.0605 0.0766

14 0.0524 0.1653 – – – 0.0161 – – 0.0242

15 0.3468 0.0323 – – – – – – –

16 0.3387 0.3065 – – – – – – –

17 0.2218 0.2702 – – – – – – –

18 0.0363 0.1371 – 0.0202 – – – – –

19 – 0.0806 – 0.0565 – – – – –

20 – 0.0081 – 0.0363 – – – – –

21 – – – 0.1411 – – – – –

21.2 – – – 0.0040 – – – – –

54 T. Chen, S. Li / Forensic Science International 146 (2004) 53–55

Department of Forensic Science, Medical College of Xi’an

Jiaotong University, Xi’an 710061, PR China.

Other remarks: All loci were found to be highly poly-

morphic and no deviation from Hardy–Weinberg equili-

brium was observed in the two ethnic groups (P ¼ 0:05).

The discrepancy of the two ethnic groups in this study is not

notable, but they are different compared to Kazak ethnic

group of China for TH01 locus. We also compared the two

ethnic groups with Han nation population and Japanese

population, respectively, there are no significant differences

observed [4–6].

This paper follows the guidelines for publication of

population data requested by the journal [7].

Acknowledgements

This Study is supported by National Nature Science

Foundation of China (grant no. 39970401). The authors

are thankful to Dr. Jianghua Lai and Yanjiong Chen for

their assistance.

References

[1] AmpF‘STR ProfilerTM PCR Amplification Kit User’s Manual.

PE Applied Biosystems, San Jose CA, 1998.

[2] Y. Ohno, I.M. Sebetan, S. Akaishi, A simple method for

calculating the probability of excluding paternity with any

number of codominant alleles, Forensic Sci. Int. 19 (1982)

93–98.

[3] M. Nei, A.K. Roychoudhury, Sampling variances of hetero-

zygosity and genetic distance, Genetics 76 (1974) 379–390.

[4] K. Yoshida, N. Mizuno, K. Fujii, H. Senju, K. Sekiguchi, K.

Kasai, H. Sato, Japanese population database for nine STR loci

of the AmpF‘STR Profiler kit, Forensic Sci. Int. 132 (2003)

166–167.

[5] X.H. Wang, S.H. Gao, J.H. Lai, S.B. Li, STR data for the

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[6] S.B. Li, J.D. Feng, S.D. Li, J.L. Yu, H.M. Yang, GeneScan

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477–484.

[7] P. Lincoln, A. Carracedo, Publication of population data of

human polymorphisms, Forensic Sci. Int. 110 (2000) 3–5.

Table 2 (Continued )


22 – – – 0.1653 – – – – –

22.2 – – – 0.0202 – – – – –

23 – – – 0.1613 – – – – –

23.2 – – – 0.0363 – – – – –

24 – – – 0.2016 – – – – –

25 – – – 0.1008 – – – – –

25.2 – – – 0.0040 – – – – –

26 – – – 0.0363 – – – – –

27 – – – 0.0161 – – – – –

H 0.7118 0.7794 0.6533 0.8677 0.6435 0.7069 0.7491 0.7897 0.8157

PD 0.8650 0.9113 0.8342 0.9629 0.8142 0.8672 0.9008 0.9277 0.9336

CE 0.5802 0.7028 0.5295 0.8630 0.4824 0.5005 0.6107 0.6854 0.7580

PIC 0.6997 0.7624 0.6468 0.8675 0.6352 0.6869 0.7217 0.7752 0.8081

H: observed heterozygosity; DP: discrimination power; CE: paternity of exclusion; PIC: polymorphism information content.

T. Chen, S. Li / Forensic Science International 146 (2004) 53–55 55

Genetic polymorphism at nine STR loci in two ethnic groups of China

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