Genetic Engineering I. Nucleotides Building blocks of DNA and RNA DNA –Genetic material...
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Transcript of Genetic Engineering I. Nucleotides Building blocks of DNA and RNA DNA –Genetic material...
![Page 1: Genetic Engineering I. Nucleotides Building blocks of DNA and RNA DNA –Genetic material –“information” storage RNA –Structural and information expression.](https://reader036.fdocuments.in/reader036/viewer/2022062422/56649edf5503460f94bef60f/html5/thumbnails/1.jpg)
Genetic Engineering I
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Nucleotides
• Building blocks of DNA and RNA
• DNA– Genetic material– “information” storage
• RNA– Structural and information expression– Catalysis– Probable first biopolymer
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Terminology
• Bases– Nitrogen containing ring
• Nucleoside– Base attached to sugar
• Nucleotide– Nucleoside + phosphate
• Nucleic Acid– Polymers of nucleotides
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A nice, convenientnumbering scheme?
Polymerize at 5’and 3’ positions
DNA is deoxy at2’ position
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Common Bases
DNA vs. RNA
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A=T or A=U pairsG=C pairs
What is effect of high GC content?
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B DNA
Other forms exist
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Biotechnology
• The discovery of restriction endonucleases (enzymes) was essential to the development of biotechnology.– Cleave at specific DNA sequences
• hundreds to thousands of bp separation
– Usually recognizes a palindromic sequence– Likely part of a host defense mechanism *– Allows you to cut and paste pieces of DNA
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Plasmids
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Today:Satellite DNAfrom E. coli picked from gel
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