Biotechnology and Genetic Engineering Genetic Engineering: Introduction Genetic Engineering.
Genetic engineering Course Overview
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Course Overview Mr. Veerendra Singh Nagoria Assistant Professor Biotechnology, School of Life Science, Rai University Ahmedabad [email protected] ,
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Transcript of Genetic engineering Course Overview
Course Overview
Mr. Veerendra Singh NagoriaAssistant Professor Biotechnology, School of Life Science, Rai University [email protected], [email protected]
Minimum Attendance requirement to sit for final semester examination is 75%
No Assignment after defined time limit Open to discussion on Web over Google
group. PPTs can be taken from my Slideshare
account but it is limited in nature consult books
Participation during lecture session will be having additional weightage
CONTINUOUS EVALUATION 30 MarksMid-Term Assessment As per examination
Assignment 1 10 MarksViva on Assignment 1 5 Marks
Assignment 2 10 MarksViva on Assignment 2 5 Marks
Class Presentation 15 Marks
Class Participation 5 Marks
END SEMESTER EVALUATION 70 Marks
Introduction to concepts, Definitions, Discoveries
Overview of fundamental processes: Replication, Transcription, Translation
Basic Methodology Restriction modification system and
Restriction Enzymes DNA modification enzymes:
Nucleases, Polymerases, Enzymes modifying the ends of DNA molecule
Vectors: Plasmids, Bacteriophages, Cosmid, Phagemid, Artificial chromosomes
Host cell types- prokaryotic and eukaryotic
Transfer of vector into host cell: Transformation, Transfection, Packaging of phage DNA, Alternative DNA delivery methods
Cloning from mRNA, Genomic DNACloning after restriction digestion;
blunt and cohesiveCreation of cloning site by PCRCloning using Linkers and AdaptersCloning by recombination; GATEWAY
technologyTA Cloning, TOPO Cloning, Ligation
free cloning
Screening methods; Chromogenic substance, Insertional inactivation, Complementation of defined mutation
Selecting recombinant clones; Reporter gene, elimination of non-transformed cells, identification of clones having Recombinant DNA
Sequence Dependent screening; Colony hybridization, Chromosome walking, Screening by PCR, Gene Tagging
Genetic Engineering & Biotechnology; protein production, protein engineering, scale up
Medicinal & Forensic Application of Gene Manipulation; Diagnosis, Treatment and DNA profiling
