8/19/2019 General Genetics Assignment 3 Question 3

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3. A piece of DNA 900 bp long is cloned and then cut out of a vector for analysis.

Digestion of this linear piece of DNA with three different restriction enzymes singly

and in all possible pairs of enzymes gave the following restriction fragment size data.

Construct a restriction map from these data.

nzymes !estriction "ragment #izes Eco !$ %00 bp& '00 bp Hin d$$$ 300 bp& (00 bp Bam )$ *0 bp& 3*0 bp& *00 bp Eco !$ + Hin d$$$ ,00 bp& %00 bp& (00 bp Eco !$ + Bam )$ *0 bp& ,*0 bp& %00 bp& *00 bp Hin d$$$ + Bam )$ *0 bp& ,00 bp& %*0 bp& *00 bp

Eco !$ + Hind $$$

Eco !$

Hind $$$

)

%00bp ,00bp (00bp

EcoRI + BamHI

EcoRI

BamHI

- -

*0bp ,*0bp %00bp *00bp

Hind $$$ + Bam )$

Hind $$$

8/19/2019 General Genetics Assignment 3 Question 3

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)

Bam )$

- -

*0bp %*0bp ,00bp *00bp

-y comparing all the restriction fragment sizes& we can construct a restriction map

from the data. he restriction map is shown as below.

/et -1 be Bam )$.

/et 1 be Eco !$.

/et )1 be Hind $$$.

- ) -

0 *0 %00 300 200 900 bp4