J ALLERGY CLIN IMMUNOL

VOLUME 127, NUMBER 2

Abstracts AB155

MONDAY

587 Dental Caries In The Primary Dentition Of Mexican ChildrenRelationship with asthma

F. Vazquez-Nava1, E. M. V�azquez-Rodr�ıguez2, M. D. Barrientos-Go-

mez3, J. A. Cordova-Fernandez4, D. Lin-Ochoa3, F. J. Beltr�an-Guzman3,

C. F. V�azquez-Rodr�ıguez3, G. M. Mart�ınez-Perales3, A. H. Sald�ıvar-Gonz�alez3; 1Social Securirty Mexican Institute. Tampico Faculty of Med-

icine. Universidad Autonoma de Tamaulipas., Ciudad Madero, MEXICO,2Tampico Faculty of Medicine. Universidad Autonoma de Tamaulipas.,

Ciudad Madero, MEXICO, 3Tampico Faculty of Medicine. Universidad

Autonoma de Tamaulipas., Tampico, MEXICO, 4Social Securirty Mexi-

can Institute. Tampico Faculty of Medicine. Universidad Autonoma de

Tamaulipas., Tampico, MEXICO.

RATIONALE: Asthma has been associated with a great number of nega-

tive health outcomes. The aimwas to study the association between asthma

and dental caries in the primary dentition.

METHODS: Data were obtained on a cohort of 1,160 Mexican children

aged 4-5 years. We used the questionnaire of the International Study of

Asthma and Allergic Diseases in Childhood (ISAAC). Classification of

asthma was based on parents’ reports. The caries index was measured as

the number of decayed (d), missing (m), and filled (f), teeth (t) (dmft) or

surfaces (dmfs). Decayed teeth included initial caries in this study.

Adjusted Odds ratios (adjusted ORs) were determined for asthma using lo-

gistic regression model. Gender, sugary products consumption, and oral

hygiene habits were utilized as covariates.

RESULTS: Dental caries prevalence was 17.9%. Approximately 226

(19.6%) children were identified with asthma. Among the children with

asthma, 166 (73.4%) suffered from asthma symptoms only during the

day and 60 (26.6%), suffered asthma symptoms during the day and night.

Prevalence of caries in children with asthmawas 19.9%. Percentage of car-

ies was higher among children who suffered nocturnal asthma symptoms

than thosewho suffered asthma symptoms only during the day. Logistic re-

gression model showed that asthma (adjusted OR51.24; 95% Confidence

interval [95% CI]50.84-1.81), was not associated with caries. We found a

significant association between nocturnal asthma symptoms (adjusted

OR51.85; 95% CI51.00-3.44) and dental caries.

CONCLUSION:Asthmawas not associated with caries. Nocturnal asthma

symptoms appear to be associated with caries in the primary dentition.

588 Sensitization to Aeroallergens in Asthmatic Children Living inRural or Urban Area in Montes Claros, Minas Gerais, Brazil

M. A. Quadros-Coelho1, C. P. Barbosa1, M. F. Silveira1, D. Sol�e2; 1UN-

IMONTES, Montes Claros, BRAZIL, 2UNIFESP-EPM, S~ao Paulo,

BRAZIL.

RATIONALE: To live in rural area is associated with less allergic sensi-

tization and allergic diseases.

OBJECTIVES: To investigate the sensitization to inhalant allergens in

children and adolescents previously assessed by standard questionnaire

from the International Study of Asthma and Allergies in Childhood

(ISAAC) and inhabiting urban or rural area.

METHODS: 551 schoolchildren (257 rural and 294 urban) aged from 6 to

14 years old, were submitted to skin prick test (SPT)withDermatophagoides

pteronyssinus (DF) Blomia tropicalis (BL), Blatella germanica (BG),

Periplaneta americana (PA), dog dander, cat dander, mix of pollens, mix

of molds, histamine and saline (FDAAllergenic�). Papulewith mean diam-

eter exceeding 3 mm characterized the SPT positive. In both areas students

were classified as asthmatic (A, affirmative answer to have had wheezing ep-

isode in the last year) and non-asthmatic (NA, negative answer).

RESULTS: The overall rate of positive SPTwas 72.4% for A and 53.5%

for NA. There were no significant differences between rural and urban

areas (urban area: A572.0% vs NA552.3%; rural: A572.7% vs

NA554.7%). The allergens most prevalent in urban areas were: DP

(53.4%), BL (47.3%), PA (23.8%) and BG (25.9%) and in rural: DP

(44.7%), BL (44.0%), BG (30.0%) and PA (23.0%).

CONCLUSION: sensitization to aeroallergens in children living in rural or

urban areas inMonteGerais is similar.Miteswere themain allergens respon-

sible for positive prick tests, found in asthmatic children in both areas.

589 Gene-gene Interactions for Asthma and BronchodilatorResponsiveness in Chinese Adults

T. F. Leung1, F. W. S. Ko2, H. Y. Sy1, H. Y. Chu2, I. H. S. Chan3, G. W. K.

Wong1, D. S. C. Hui2; 1Department of Pediatrics, The Chinese University

of Hong Kong, Hong Kong, HONG KONG, 2Department of Medicine and

Therapeutics, The Chinese University of Hong Kong, Hong Kong, HONG

KONG, 3Department of Chemical Pathology, The Chinese University of

Hong Kong, Hong Kong, HONG KONG.

RATIONALE: Asthma is a complex disease resulting from interactions

between multiple candidate genes. b2-agonists, being widely used anti-

asthma drugs, relieve bronchoconstriction and asthma symptoms.

Genetic study for spirometric parameters could help to predict responses

to b2-agonists.METHODS:We investigated the interactions among 15 single-nucleotide

polymorphisms (SNPs) in 8 candidate genes for asthma diagnosis and

bronchodilator responsiveness (BDR) in 345 Chinese asthmatics and 464

non-allergic controls. Generalized multifactor dimensionality reduction

(GMDR) was used to analyze gene-gene interactions for asthma

phenotypes.

RESULTS:Asthma diagnosis was associated with rs7216389 inORMDL3and rs3756780 in ARG1 (P50.039 and 0.007 respectively), whereas BDR

was associated with rs1007654 in GSDM1, rs1003929 in CRHR2 and

rs2749935 in ARG1 (P50.020-0.049). When analyzed by GMDR,

rs7216389 in ORMDL3 and rs3756780 in ARG1 interacted significantly

for asthma risk (P50.006). Subjects with high-risk genotypes formed by

these two genes had odds ratio 1.66 (95% confidence interval [CI] 1.24-

2.23, P50.0005) for asthma when compared with those with low-risk gen-

otypes. One two-locus model consisting of rs2749935 in ARG1 and

rs2190242 in CRHR2 was associated with BDR (P50.007). Specifically,

FEV1 reversibility following salbutamol inhalation was higher in high-

risk patients than low-risk patients (mean [95% CI]: 10.7 [8.6-12.9] %

vs 6.8 [5.9-7.6] %; P50.001), the latter group also higher than that of

high-risk controls (2.8 [1.4-4.3] %; P<0.0001).

CONCLUSIONS:Asthma is associated with ARG1 and SNPs on chromo-

some 17q21 in Chinese adults. Significant gene-gene interactions were

found between ARG1 and ORMDL3 for asthma, and between ARG1 and

CRHR2 for BDR in asthmatic patients.

590 Correlates Of Repeated Admissions For Asthma In An UrbanTertiary Care Hospital In Saudi Arabia

A. Hasan, E. Yousef; King Faisal Specialist Hospital &Research Centre,

Jeddah, SAUDI ARABIA.

RATIONALE: The purpose of this investigation was to determine the risk

factors for repeated hospital admissions for asthma in an urban setting in

Saudi Arabia.

METHODS:Medial Records of athamatic patients whowere hospitalized

two or more times between 2000 and 2010 were reviewed. A questionnaire

was completed for each admission for 44 patients aged 2-70 years.

RESULTS: The results demonstrated a male-to-female ratio of 1:5, with a

mean age of 26 years. All the subjects were residing in urban environment

in one of the largest city in Saudi Arabia. The mean number of hospital ad-

missions was 3.6. Noncompliance was noted in 62%. 32% were exposed to

smoke. Medical records revealed following active triggers; URI (83%), non-

compliance at the time of admission (62%), change in weather conditions

(52%). In addition 58% were having heart or joint problems requiring

Aspirin. 21% had history of depression.Medications at time of admission in-

cluded albuterol (100%), inhaled corticosteroids (79%, however was started

only within 48 hrs prior to admission) and oral steroids (59%). 28% percent

of the subjects had their asthmamanaged by specialists(Allergist and pulmo-

nologists) compared to seventy two percent subjects whose asthma was

managed by generalists (Family physicians, Internists and Pediatricians).

CONCLUSIONS: These data suggest that repeated hospitalization due to

asthma in patients residing in urban area in Saudi Arabia was associated

with common occurrence in female patients, URI, change in weather,

non compliance, lack of access to asthma specialists, smoke exposure, car-

diovascular problems and use of aspirin.