Gene Engineering Division - Riken
Transcript of Gene Engineering Division - Riken
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Head
Gene Engineering Division
Goal
Activities
The Gene Engineering Division (RIKEN DNA Bank) is a
nonprofit resource archive that provides genetic resources,
technical services and educational program to qualified
investigators in private industries, governments and academic
organizations around the world. RIKEN DNA Bank has been
selected as a central facility for collecting, preserving and
delivering DNAs of animals and microorganisms through
the National BioResource Project sponsored by the Ministry
of Education, Culture, Sports, Science and Technology of
JAPAN (MEXT). Our division conducts research to ensure
1. Collection, preservation and distribution of genetic
resources
The Gene Engineering Division (RIKEN DNA Bank) is
divided into seven sections for DNA Banking.
(1) Cloned-DNA Set Bank. This handles a cloned collection
of full-length cDNAs assembled with specific research
areas such as hormones, cytokines, apoptosis, cell cycle,
signal cascades, transcription factors, replication factors,
and ubiquitination. These representative cloned sets were
isolated from cDNA libraries, phage, cosmid, BAC, YAC,
PAC, P1 and phosmid libraries.
(2) Japanese-Specific DNA Bank. This handles human HLA
class I clones that are unique to Japanese and SEREX
clones coding cancer antigens of Japanese origin and to
HistoryRIKEN DNA Bank was established in June 1987 when a
committee of scientists recognized the need for a central
collection of recombinant DNA that would serve scientists in
Asia. In 2001, RIKEN BioResource Center (RIKEN BRC)
was established and then the DNA Bank was reorganized into
the Division of Gene Engineering. In 2002, the division was
Kazunari K. YOKOYAMA, Ph.D.
the authenticity of the genetic materials in the collection, and
to improve and standardize the methods of characterization,
maintenance, preservation and distribution of genetic
materials. We distribute cloned DNAs, gene libraries
(e.g., cDNAs, phages, cosmids, BAC, phosmids, and YAC
libraries), vectors, hosts, recombinant viruses and ordered
library sets from humans, mice, microorganisms, viruses
and other animals. Our division also performs and sponsors
research to improve and standardizes for the advancement,
validation and application of scientific knowledge.
recognized as the central archive for the collection of “animal
DNA and microorganism DNA” in the National BioResource
Project (NBRP) program (Phase I), sponsored by MEXT. In
2007, it was also recognized as the central archive for the
collection of “DNA” in the NBRP (Phase II), sponsored by
MEXT.
other clones for Japanese heredity.
(3) Recombinant-Virus Bank. This handles recombinant
viruses constructed by inserting a full-length cDNA into
viral vectors, generating the viral particles as resources.
The viruses are examined of their qualities by methods
developed in our division. The DNA fragments derived
from human and mouse full-length cDNA libraries were
used as donors of recombinant viruses.
(4) Promoter Bank. This handles promoter DNA fragments
fused to reporter genes such as luciferase, lacZ, GFP and
Cre recombinase. Transgenic promoter Cre mice are also
generated by collaborating with the Animal Resource
Center of Tsukuba University and the Experimental
Animal Division of RIKEN BRC. The second phase of
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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MembersHead
Kazunari K. YOKOYAMA, Ph.D. (2001.4~)
Senior Research Scientist
Takehide MURATA, Ph.D. (2001.4~) Jianzhi PAN, Ph.D. (2005.4~)
Makoto KIMURA, Ph.D. (2003.7~2007.9)
Contract Researcher
Koji NAKADE, Ph.D. (2004.1~)
Visiting Scientist
Tetsuo HIRANO, Ph.D. (2001.4~) Michiya NOGUCHI, Ph.D. (2008.5~)
JRA (~2008.3)
Visiting Technician
Tsuyoshi AOKI (2008.4~)
Technical Staff Ⅱ
Kumiko INABE (2002.4~) Takahito YAMASAKI (2003.4~)
Yukari KUJIME (2004.4~) Megumi HIROSE (2004.4~)
Satoko MASUZAKI (2006.4~) Sanae YAMAOKA (2004.4~2008.3)
Agency Staff
Kazuko UENO (2001.4~)
Carlo Dante T.NATIVIDAD, Ph.D. (2008.4~2008.11)
this Bank is to address the promoters of p53-related genes
with reporter constructs.
(5) Archives of large-genome cloned library. This handles
cDNA and genome libraries from various animals. This
section was based on the NBRP for collecting all genome
resources produced by the NBRP (Phases I and II) such
as those from Xenopus, Japanese monkeys, rats, mice,
humans, yeasts, Ciona and Thermus thermophilus.
(6) Basic domain of DNA Bank. This handles individual
cDNAs, genome DNA clones and vectors as well as host
cells.
(7) Bioinformatics section. This handles the informatics of our
genetic resources for DNA Banking.
We distribute genetic resources to only qualified researchers
associated with certain research, medical or educational
organizations. We also report the activities of our division in
an annual report, qualified by the “Resource Committee”. We
also discuss the future plan of our mission. The “Resource
Ethics Committee” confirms the banking activity of genetic
resources of humans every year. The “Advisory Council”
is held to evaluate the activities of RIKEN BRC every other
year. We evaluate not only the activities of our DNA Banking
but also the research activities of developing technologies
related to DNA Banking.
2. Development of new technology to ensure the
authenticity of genetic materials
The development and improvement of methods for the
standardization and characterization of genetic resources
are also conducted by our division. These technologies,
as described below, are necessary and entail the following:
1) identification of mutation sites in genetic resources; 2)
preparation of novel vectors and an adenovirus vector for
the controlled expression system of genes; 3) preparation of
an artificial reporter vector with different cis-elements and
promoter-reporter constructs; 4) development and validation
of new gene-transferring system using targeted promoter/
enhancer element; 5) development of a new system for
studying gene expression in eukaryotic cells and animals; and
6) production of modified proteins in Escherichia coli on a
large scale.
3. Education and training of scientists.
Our division offers a training program for young scientists and
students that teaches the best use of adenoviral vectors.
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Specific Aims
I. Collection, preparation and distribution of genetic materials.
1. Banking system
We have collected the following numbers of genetic materials:
host, vector, cloned DNA, 3,451; Nakamura-White RELP
marker clone, 123; human genomic YAC clone library,
35,712; human full length cDNA cloned library, 307,200;
mouse 15 K cDNA cloned library, 15,000; mouse 7.4 K
cDNA cloned library, 7,407; mouse cDNA cloned library,
45,216; cDNA library, 47; mouse BAC cloned library,
193,152; human SEREX clone, 584; Japanese HLA class I,
40; recombinant virus, 502; NBRP Japanese macaque genome
library, 200,064; Tokushima Univ. mouse cDNA cloned
library, 374,208; common marmoset cDNA cloned library,
353,664; chimpanzee 22nd chromosome genome library,
13,824; NBRP Xenopus laevis cDNA cloned library, 186,400;
NBRP Xenopus tropicalis cDNA cloned library, 44,544;
cricket cDNA cloned library, 60,288; Thermus thermophilus
HB8 expression/KO plasmids, 2,753; Schizosaccharomyces
pombe cDNA cloned library, (ORFeome library) 14,436;
human genome library, 399,456, and Ciona intestinalis cDNA
cloned library, 452,352.
Operation of automated DNA-extraction machine
Masato OKUBO (2008.4~) Chitose KURIHARA (2008.4~)
Yuri NAKANO (2008.4~) Yuka KUSA (2008.4~)
Miyuki YAMAMOTO (2003.6~2008.4) Reiko OHTO (2007.4~2008.4)
Student Trainee
Rei KAWASHIMA, M.D. (2005.4~) Shinji ENDO (2007.4~)
Atsushi HIGA (2007.5~2008.2) Mariko WAKAYAMA (2002.4~2007.3)
Yusuke IDE (2005.4~2006.2)
Nakade, Arita, Katsuya, Kusa, Inabe, Hirose, Masuzaki, Nakajima, Sato, Kimura, NoguchiKurihara, Kujime, Fujisawa, Takahara, Hattori, Hiraguri, Ueno, Nakano, Furuya
Natividad, Kawashima, Pan, Yokoyama, Murata, Yamasaki, Okubo
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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Operation of centrifugation
Operation of PCR amplification machine
Confirmation of nucleotide sequence by DNA sequencing
2. An E-mail News 34-72 version is sent to users.
3. Number of users registered to RIKEN DNA Bank is
6,651.
4. DNA materials (241,479) have been distributed to users
worldwide.
5. The size of the DNA collection archives is now third in
the world.
6. The Cre-zoo project was completed in 2008.
II. Technological development We have performed the following research projects to develop
a new technology for DNA Banking.
1. Detection of mutation of DNA samples
We have developed novel techniques for detecting the
mutation of genetic resources with high sensitivities and
reproducibilities. These techniques are used for validating the
quality of genetic resources.
2. Development of controlled expression system for genes
with modification enzymes
Recent progress in recombinant DNA technology focused on
the modification system for genes such as epigenesis, protein
degradation, phosphorylation and the addition of sugar and
lipid moieties to core proteins and DNAs. We have developed
a new two-vector or one-vector system for modifying gene
products with genes encoding methylation/demethylation-,
kinase/phosphatase-, acetylase/deacetylase-, ubiquitination/
deubiquitination-, sumoylation/desumoylation- and sugar/
lipid-related enzymes.
3. Development of a system for targeted gene delivery
using a specific promoter and generation of transgenic
mice with controlled gene expression
We have developed a regulated gene expression system using
reporter constructs of tissue-specific promoters and generated
novel transgenic mice with a Cre-loxP cassette with the tissue-
specific promoter (in collaboration with Tsukuba University
and the Experimental Animal Division of BRC). We are now
focusing on the promoters of the p53-targeted gene family.
4. Application of adenovirus vectors to cancer gene
therapy, regeneration biology and molecular biology
We have developed a novel gene delivery system for
cancerous cells and embryonic stem cells as well as for model
animals using tumor suppressor genes and suicide genes. We
have also developed an efficient system for gene transfer using
novel adenoviral vectors with E1-Rb mutants, chimeric fibers
and modified fibers for gall bladder cancer, biliary tract cancer
and liver cancer (in collaboration with Tsukuba University and
Sapporo Medical University). We have focused on embryonic
stem cells for the gene delivery of modified adenoviral
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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vectors.
5. Development of gene expression system and evaluation
system of expressed genes in eukaryotic cells and animal
models
We have developed a novel strategy for evaluating gene
expression in chromatin and for evaluating the system for
expressed gene products in cells. We focused on the AP-1
family of transcription factors and on chromatin-modified
factors such as those involved in histone acetylation and
methylation as well as in sumoylation in eukaryotic cells and
model animals.
6. Efficacy of artificial promoter vectors
A controlled reporter system with the DNA binding sites
(cis-elements) of transcription factors has been developed
and examined of their efficacies for various cells including
neoplastic, normal diploid, and embryonic stem cells as well
as germ cells.
III. Evaluation of activities of the “National BioResource Project (NBRP; phase I)”.
The NBRP committee evaluated the banking activities of
RIKEN BRC with the highest score “S” among those of other
groups.
Administration care of MTA for Banking
Development of technology using Promoter-GFP DNA (Promoter Bank)
Initiation of adipocyte differentiation of MEF-JDP2(-/-) (DNA-set Bank)
IV. Introduction and distribution of our banking activities
We have set up the homepage http://www.brc.riken.jp/dna/en/
index.html and connected with the database of the National
Institute of Genetics. We have made e-mail news, catalogs and
other related notices of our DNA Bank in RIKEN BRC for
researchers worldwide. We have also introduced our banking
activities in annual domestic conferences such as those of the
Molecular Biology, Biochemistry, Cancer, Gene Therapy, as
well as in some international conferences such as the Cold
Spring Harbor meetings.
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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Saito S., Sawai K., Minamihashi A., Ugai H., Murata
T., Yokoyama K.: “Derivation, maintenance and
differentiation of equine ES cells.” Nonhuman embryonic
steme cell. Protocols; vol. 1 (ed. by K. Turksen) (Humana
Press Inc., Totowa, NJ), Methods in Molecular Biology
329, pp.59-79 (2005).*
Seo E., Abei M., Wakayama M., Fukuda K., Ugai
H., Murata T., Todoroki T., Tanaka N., Hamada H.,
Yokoyama K.: “Effective gene therapy for biliary
tract cancer by a conditionally replicative adenovirus
expressing uracil phosphoribosyltransferase: Significance
of timing of 5-fluorouracil administration.” Cancer
Research 65, 546-552 (2005).*
Yokoyama K., Murata T., Ugai H., Suzuki E., Terashima
M., Kujime Y., Inamoto S., Hirose M., Inabe K.,
Yamasaki T.: “Recombinant Virus BANK for gene
delivery.” Science 307, 1722 (2005).*
Ugai H., Yamasaki T., Hirose M., Inabe K., Kujime Y.,
Terashima M., Liu B., Tang H., Zhao M., Murata T.,
Kimura M., Pan J., Obata Y., Hamada H., Yokoyama
K.: “Purification of infectious adenovirus in two hours
by ultracentrifugation and tangential flow filtration.”
Biochem. Biophys. Res. Commun. 331, 1053-1060
(2005).*
Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki E.,
Nakata H., Kujime Y., Inamoto S., Hirose M., Inabe K.,
Terashima M., Yamasaki T., Liu B., Nakade K., Pan J.,
Kimura M., Saito I., Hamada H., Obata Y., Yokoyama
K.: “A database of recombinant viruses and recombinant
viral vectors available from the RIKEN DNA bank.” J.
Gene Med. 7, 1148-1157 (2005).*
Dietze E. C., Bowie M. L., Mrozek K., Caldwell E., Neal
C., Marjoram R. J., Troch M. M., Bean G. R., Yokoyama
K., Ibarra C. A., Seewaldt V. L.: “CREB-binding protein
regulates apoptosis and growth of HMECs grown in
reconstituted ECM via laminin-5.” J. Cell Science 118,
5005-5022 (2005).*
Publications
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【Original Papers】 (*Peer reviewed journals)
Kojima Y., Honda K., Kotegawa H., Kushihata F.,
Kobayashi N., Yokoyama K., Hamada H.: “Adenovirus-
mediated p53 gene transfer to the bile duct by direct
administration into the bile in a rat cholangitis model.” J.
Surgical Res. 128, 126-131 (2005).*
Ugai H., Inabe K., Yamasaki T., Murata T., Obata Y.,
Hamada H., Yokoyama K.: “Accumulation of infections
mutants in stocks during the propagation of fiver-
modified recombinant adenoviruses.” Biochem. Biophys.
Res. Commu. 337, 806-814 (2005).*
Yamasaki T., Noguchi M., Liu B., Nakade K., Wang P-C.,
Murata T., Yokoyama K.: “Jun dimenigation protein 2 : A
multifunctional transcription factor in mammalian cells.”
Current Genomics 6, 351-364 (2005).*
Mitsuzawa H., Kimura M., Kanda E., Ishihama A.:
“Glyceraldehyde-3-phosphate dehydrogenase and actin
associate with RNA polymerase II and interact with its
Rpb7 subunit.” FEBS Letters 579, 48- 52 (2005).*
Hayashi K., Watanabe T., Tanaka A., Furumoto T., Sato-
Tsuchiya C., Kimura M., Yokoi M., Ishihama A., Hanaoka
F., Ohkuma Y.: “Studies of Schizosaccharomyces pombe
TFIIE indicate conformational and functional changes in
RNA polymerase II at transcription initiation.” Genes to
Cells 10, 207-224 (2005).*
Abiko M., Akibayashi K., Sakata T., Kimura M.,
Kihara M., Itoh K., Asamizu E., Sato S., Takahashi H.,
Higashitani A.: “High-temperature induction of male
sterility during barley (Hordeum vulgare L.) anther
development is mediated by transcriptional inhibition.”
Sex Plant Reprod 18, 91-100 (2005).*
Wang Y., Onishi Y., Kakinuma N., Roy BC., Aoyagi T.,
Kiyama R.: “Alternative splicing of the human Kank
gene produces two types of Kank protein.” Biochem.
Biophys. Res. Commun. 330, 1247-1253 (2005).*
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Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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Saito S., Yokoyama K., Tamagawa T., Ishiwata I.:
“Derivation and induction of the differentiation of animal
ES cells as well as human pluripotent stem cells derived
from fetal membrane.” Human Cell 18, 135-141 (2005).*
Liu B., Yamasaki T., Noguchi M., Murata T., Wang P.,
Yokoyama K.: “Gene silencing and histone modification
by JDP2, a AP-1 repressor during the differentiation of
embryonic stem cells.” Current Topics in Genetics 1,
1-17 (2006).*
Da L., Yokoyama K., Li T., Zhao M.: “Dual promoters
control the cell-specific expression of the human cell
death-inducing DFF45-like effector B gene.” Biochem. J.
393, 779-788 (2006).*
Jin C., Kato K., Chimura T., Yamasaki T., Nakade K.,
Murata T., Li H., Pan J., Zhao M., Sun K., Chiu R., Ito
T., Nagata K., Horikoshi M., Yokoyama K.: “Regulation
of histone acetylation and nucleosome assembly
by transcription factor JDP2.” Nature Structural &
Molecular Biology 13, 331-338 (2006).*
Hayashi R., Goto Y., Ikeda R., Yokoyama K., Yoshida
K.: “CDCA4 is an E2F transcription factor family-
induced nuclear factor that regulates E2F-dependent
transcriptional activation and cell proliferation.” J. Biol.
Chem. 281, 35633-35648 (2006).*
Lu Y-C., Song J., Cho H-Y., Fan G., Yokoyama K., Chiu
R.: “Cyclophilin a protects peg3 from hypermethylation
and inactive histone modification.” J. Biol. Chem. 281,
39081-39087 (2006).*
Wakayama M., Abei M., Kawashima R., Seo E., Fukuda
K., Ugai H., Murata T., Tanaka N., Hyodo I., Hamada H.,
Yokoyama K.: “E1A, E1B double-restricted adenovirus
with RGD-Fiber modification exhibits enhanced
oncolysis for CAR-deficient biliary cancers.” Clinical
Cancer Research. 13, 3043-3050 (2007).*
Nakade K., Pan J., Yoshiki A., Ugai H., Kimura
M., Liu B., Li H., Obata Y., Iwama M., Itohara S.,
Murata T., Yokoyama K.: “JDP2 suppresses adipocyte
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differentiation by regulating histone acetylation.” Cell
Death and Differentiaton 14, 1398-1405 (2007).*
Noguchi M., Yamasaki T., Masuzaki S., Higa A., Nakade
K., Pan J., Murata T., Nagata K., Shiota K., Yokoyama
K.: “Transcription factor Jun dimerization protein 2
(JDP2) has histone-chaperone activity that suppresses
gene expression.” Current Topics in Biochemical
Research 9, 27-44 (2007). *
Yamasaki T., Murata T., Jin C., Kato K., Noguchi M.,
Nakade K., Pan J., Nagata K., Yokoyama K.: “Assays
of nucleosome assembly and the inhibition of histone
acetyltransferase activity.” Nature Protocols DOI:
10.1038/nprot.2007.332 (2007).*
Saito S., Sawai K., Murayama Y., Fukuda K., Yokoyama
K.: “Nuclear Transfer to study the nuclear reprogramming
of human stem cells.” Methods in Molecular Biology
438, 151-169 (2008). *
Murata T., Shinozuka Y., Obata Y., Yokoyama K.:
“Phosphorylation of two eukaryotic transcription factors,
Jun dimerization protein 2 and activation transcription
factor 2, in Escherichia coli by Jun N-terminal kinase 1.”
Anal. Biochem. 376, 115-121 (2008).*
Hirano T., Ike F., Murata T., Obata Y., Utiyama H.,
Yokoyama K.: “Genes encoded within 8q24 on the
amplicon of a large extrachromosomal element are
selectively repressed during the terminal differentiation
of HL-60 cells.” Mutation Research 640, 97-106 (2008).*
Haase M. G., Klawitter A., Bierhaus A., Yokoyama
K., Kasper M., Geyer P., Baumann M., Baretton G.
B.: “Inactivation of AP1 Proteins by a Nuclear Serine
Protease Precedes the Onset of Radiation-Induced
Fibrosing Alveolitis.” Radiation Research 169, 531-542
(2008).*
Kovacevic-Grujicic N., Yokoyama K., Stevanovic M.:
“Trans-activation of the human sox3 promoter by mazin
NT2/D1 cells.” Arch. Biol. Sci., Belgrade 60, 379-387
(2008).*
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Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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Kimura M.: “IRF2-binding protein-1 is a JDP2 ubiquitin
ligase and an inhibitor of ATF2-dependent transcription.”
FEBS Letters 582, 2833-2837(2008).*
Honda A., Hirose M., Inoue K., Ogonuki N., Miki H.,
Shimozawa N., Hatori M., Shimizu N., Murata T., Hirose
M., Katayama K., Wakisaka N., Miyoshi H., Yokoyama
K., Sankai T., Ogura A.: “Stable embryonic stem cell
lines in rabbits: potential small animal models for
human research.” Reproductive BioMedicine Online 17,
706-715 (2008).*
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Fukuda K., Abei M., Kawashima R., Ugai H., Seo E.,
Wakayama M., Murata T., Endo S., Hamada H., Hyodo I.,
Yokoyama K.: “E1A, E1B-double restricted replicative
adenovirus at low dose augments the efficacy of tumor-
specific suicide gene therapy for gallbladder cancer.”
Cancer Gene Therapy (in press).*
Sato I., Obata Y., Kasahara K., Nakayama Y., Fukumoto
Y., Yamasaki T., Yokoyama K., Saito T., Yamaguchi, N.:
“Differential trafficking of c-Src, Lyn, c-Yes, and Fyn
is specified by the state of palmitoylation in the SH4
domain.” J. Cell Science (in press).*
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Oral Presentations
Yokoyama K., Nakade K., Pan J., Liu B., Yamasaki T.,
Kimura M., Abei M., Murata T.: “Histone acetylation
and de-acetylation is critical for cell-differentiation in
response to retinoic acid (RA).” International Symposium
on Molecular cell Biology of Macrophages 2005, Omiya,
Saitama, Japan, pp.112, Jun. (2005).
Nakade K., Pan J., Liu B., Yamasaki T., Kimura
M., Murata T., Yokoyama K.: “Inhibition of histon
acetylation by transcription factor JDP2 is critical during
differentiation of F9 cells.” 70th Symposium, Molecular
Approaches to Controlling Cancer, Cold Spring Harbor,
NY. pp.166, Jun. (2005).
Yokoyama K., Yamasaki T., Liu B., Nakade K., Pan J.,
Kimura M., Murata T.: “Inhibition of histon acetylation
by transcription factor JDP2 is critical for differentiation
of F9 cells.” Mechanisms of Eukaryotic Transcription,
Cold Spring Harbor Meeting, Cold Spring Harbor, NY,
pp.321, Aug. (2005).
Murata T., Ugai H., Inabe K., Pan J., Hamada H.,
Yokoyama K.: “Genotypic study of accumulated
infectious mutants in stocks during the preparation
of fiber-modified recombinant adenoviruses.” Target
Definition & Vector Design, Cold Spring Harbor
Meeting, Cold Spring Harbor, NY, pp. 31 Nov. (2005).
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Liu B., Ugai H., Pan J., Murata T., Hamada H.,
Yokoyama K.: “Purification of infectious adenovirus
in two hours by ultracentrifugation and tangential flow
filtration.” Target Definition & Vector Design, Cold
Spring Harbor Meeting, Cold Spring Harbor, NY, pp. 27
Nov. (2005).
Yokoyama K., J in C., Yamasaki T. , Murata T.:
“Regulation of histone acetylation and nucleosome
assembly by transcription factor JDP2.” International
Symposium on Molecular Cell Biology of Macrophages
and Dendritic cells 2006, Hongo, Tokyo, Japan, pp.102,
Jun. (2006).
Yokoyama K., Yamasaki T., Noguchi M., Ide Y., Liu B.,
Wang Y., Nakade K., Pan J., Kimura M., Jin C., Murata
T.: “Inhibition of histone acetylation by transcription
factor JDP2 is critical for differentiation of F9 cells.”
20th IUBMB International Congress of Biochemistry and
Molecular Biology and 11th FAOBMB Congress, Kyoto,
Japan, pp.770, Jun. (2006).
Murata T., Terashima M., Pan J., Kimura M., Yokoyama
K.: “Establishment of the Recombinant Virus Database
and the gene delivery system LICCA.” 20th IUBMB
International Congress of Biochemistry and Molecular
Biology and 11th FAOBMB Congress, Kyoto, Japan,
pp.323, Jun. (2006).
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【International Conferences】
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
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Kimura M., Ishihama A., Yokoyama K.: “Structure-
function analysis of the Rpb4 subunit of RNA
polymerase II in Schizosaccharomyces pombe.” 20th
IUBMB International Congress of Biochemistry and
Molecular Biology and 11th FAOBMB Congress, Kyoto,
Japan, pp.236, Jun. (2006).
Pan J., Murata T., Nakade K., Liu B., Yoshiki A., Itohara
S., Yokoyama K.: “AP-1 repressor JDP2 control cell
proliferation by regulating the cell cycle.” 20th IUBMB
International Congress of Biochemistry and Molecular
Biology and 11th FAOBMB Congress, Kyoto, Japan,
pp.715, Jun. (2006).
Nakade K., Pan J., Yoshiki A., Ugai H., Murata T.,
Yokoyama K.: “JDP2 controls adipogenesis by regulating
the expression of C/EBPdelta via histone acetylation.”
20th IUBMB International Congress of Biochemistry and
Molecular Biology and 11th FAOBMB Congress, Kyoto,
Japan, pp.246, Jun. (2006).
Wang Y., Murata T., Pan J., Yokoyama K.: “Subcellular
localization of mouse JDP2 protein.” 20th IUBMB
International Congress of Biochemistry and Molecular
Biology and 11th FAOBMB Congress, Kyoto, Japan,
pp.623, Jun. (2006).
Liu B., Inabe K., Noguchi M., Yamasaki T., Shiota K.,
Murata T., Yokoyama K.: “Gene therapy of neoplastic
cancer cells by a conditionally replicative adenovirus
expressing transcription factor JDP2.” 20th IUBMB
International Congress of Biochemistry and Molecular
Biology and 11th FAOBMB Congress, Kyoto, Japan, pp.
246, Jun. (2006).
Yamasaki T., Noguchi M., Ide Y., Liu B., Yamaguchi N.,
Shiota K., Murata T., Yokoyama K.: “Analysis of DNA-
Binding domain, Histone-Binding domain and domain of
inhibition of histone acetyltransferase (INHAT) of JDP2
for transcriptional regulation.” 20th IUBMB International
Congress of Biochemistry and Molecular Biology and
11th FAOBMB Congress, Kyoto, Japan, pp.246, Jun.
(2006).
Wakayama M., Kawashima R., Abei M., Murata T.,
Hamada H., Yokoyama K.: “Enhanced Oncolysis and
Safety of a Conditionally Replicative Adenovirus (CRAd)
with RGD Fiber Modification for Biliary Cancers.” 20th
IUBMB International Congress of Biochemistry and
Molecular Biology and 11th FAOBMB Congress, Kyoto,
Japan, pp.593, Jun. (2006).
Noguchi M., Yamasaki T., Murata T., Liu B., Pan J.,
Kimura M., Shiota K., Yokoyama K.: “Analysis of
the mechanism of histone acetylation regulated by
transcription factor JDP2.” 20th IUBMB International
Congress of Biochemistry and Molecular Biology and
11th FAOBMB Congress, Kyoto, Japan, pp.245, Jun.
(2006).
Yokoyama K., Jin C., Yamasaki T., Noguchi M., Murata
T.: “Regulation of histone acetylation and nuclosome
assembly by transcription factor JDP2.” Mechanisms &
Models of Cancer, Cold Spring Harbor Meeting, Cold
Spring Harbor, NY, pp.305, Aug. (2006).
Pan J., Murata T., Kimura M., Nakade K., Inabe K.,
Yamasaki T., Kujime Y., Hirose M., Yamamoto M.,
Yamaoka S., Yokoyama K.: “RIKEN DNA Bank,
A Global Recombinant DNA Depository Institution
For Advancing Post-genomic Research.” 4th ISGO
International Conference on Structural Genomics,
Beijing, China, pp.188, Oct. (2006).
Pan J., Nakade K., Yamasaki T., Murata T., Yokoyama
K.: “AP-1 repressor JDP2 suppresses adipocyte
differentiation by regulating histone acetylation.” 16th
International Symposium on Molecular Cell Biology of
Macrophages 2007, Shizuoka, Japan, pp.75, Jun. (2007).
Pan J., Nakade K., Yamasaki T., Kimura M., Murata
T., Yokoyama K.: “AP-1 repressor JDP2 suppresses
adipocyte differentiation by regulating histone
acetylation.” Mechanisms of Eukaryotic Transcription,
Cold Spring Harbor Meeting, Cold Spring Harbor, NY,
pp.179, Aug. (2007).
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
Gene Engineering DivisionRIKEN BRC Annual Report 2005 ~ 2007
― 74 ―
Yokoyama K., Nakade K., Pan J., Murata T.: “AP-1
repressor JDP2 is histone chaperone to repress the viral
promoter activity.” 7th Awaji International Forum on
Infection and Immunity, Awaji, Japan, pp.74, Sep. (2007).
Nakade K., Pan J., Yamasaki T., Murata T., Yokoyama
K.: “AP-1 repressor JDP2 regulates differentiation and
proliferation of adipocytes and replicative senescence of
mouse embryonic fibroblasts.” Mechanisms & Models
of Cancer, Cold Spring Harbor Meeting, Cold Spring
Harbor, NY, pp.177, Aug. (2008).
Yokoyama K., Pan J. Nakade K., Murata T.: “Anti-viral
factor JDP2 regulates differentiation and proliferation
of adipocytes and replicative senescence.” 8th Awaji
International Forum on Infection and Immunity, Awaji,
Japan, pp.68, Sep. (2008).
21.
22.
23.
【Domestic Conferences】 Total 60