Gases Exchange in Plants 1

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    GASES

    EXCHANGE IN

    PLANTSPRESENTED BY :Siti Sarah binti Sekeri

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    Introduction

    Gas exchange three major gases with the

    environment

    These are CO (for photosynthesis), O(for respiration) and water vapour

    (transpiration)

    These gases may be taken up or releaseby plants

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    During the day, most plants

    produce oxygen through

    photosynthesis and use up

    carbon dioxide During the night, when there is

    no photosynthesis, oxygen isconsumed for respiratory

    processes

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    In aquatic plants, carbon dioxide

    required for photosynthesis is

    obtained from the surroundingwater in the form of hydrogen-

    carbonate ions from the carbonicacid present

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    In terrestrial plants, the carbondioxide is obtained from the

    atmosphere through the stomata

    found on the surface of leaves,

    stems and branches

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    Structure of Stoma

    Stoma is a pore or aperture that

    penetrates the epidermis of leaves,

    branches and stems of green plants Normally, more stomata are found

    on the lower epidermis compared to

    the upper epidermis of leaves, and

    much less on the epidermis of stems

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    Gaseous exchange in plants

    occurs mainly through porescalled stomata

    They are found on the epidermis

    of leaves & stems of flowering

    plants

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    Lenticels found in the bark ofstem & root hairs with thin walls

    & large surface are also allowgaseous exchange to take place

    Each stoma consist of a stomatapore surrounded by two guardcells

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    Each guard cell is kidney-shapes

    & contains chloroplasts

    It has a thinner outer wall & a

    thicker, less elastic, inner wall

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    Changes in turgid pressure of

    guard cells causes the opening or

    closing of the stomatal pore

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    Besides, stoma is a formed by

    two specialized epidermal cells,

    bordering each other The guard cells play an important

    role in the opening and closing ofthe stoma

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    Next, the guard cells are bean-

    shaped, and are different fromother epidermal cells

    These guard cells contain

    chlorophyll, thus can

    photosynthesise, whereas the

    epidermal cells do not containchlorophyll and thus cannot

    photosynthesise

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    Another unique characteristic ofthe guard cell is that the inner

    cellulose wall bordering thestoma is thicker than the outercellulose wall bordering the

    epidermal cells around the guardcells

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    Functions of Stoma

    Stoma plays important role in the

    gaseous exchange of respiratory

    gases, in particular theabsorption of CO, and the

    regulation of water in the plants

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    O produced during

    photosynthesis diffuses outthrough the stomatal pores into

    the atmosphere, while COdiffuses in from the atmosphere

    into the leaves

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    Under water deficit conditions,

    stoma closes automatically

    This helps to prevent water loss

    from the plants until the waterdeficit diminishes

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    STOMATAL

    OPENING

    STOMATALCLOSING

    STOMATAL

    OPENING

    AND

    CLOSING

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    STOMATAL OPENING

    It has been observed that stomatal opening is

    promoted by high light intensity and low

    mesophyll CO levels.

    Guard cells, which contain chloroplasts,

    generate ATP by photophosphorylation during

    photosynthesis.

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    Blue light (BL) is absorbed by

    BL photoreceptors

    Which activate a proto pump

    (H-ATPase) in the cell

    membrane of the guard cell.

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    ATPs generated by the light-

    dependent reaction ofphotosynthesis are hydrolysed to

    provide energy to drive the proton

    pump. As proton (H) are pumped out of

    the guard cells, the cells become

    increasingly negatively charge.

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    Potassium channels are activated

    and K ions diffuse fromsubsidiary cells through the

    channels down this

    electrochemical gradient into the

    guard cells.

    Chloride ions (Cl) then enter tobalance the charge.

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    In some plants, starch are

    converted into malate (the oldhypothesis proposed that

    starch was converted tosugar).

    The older hypothesis is thestarch-sugar hypothesis.

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    During the daylight, starch is

    converted into soluble sugars

    to lower the water potential of

    cell sap.

    The reverse process occurs at

    night.

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    The accumulation of K ions

    (and malate ions) causes thewater potential in the guard

    cells to become more

    negative.

    Water enters by osmosis from

    neighbouring subsidiary cells

    into the guard cells.

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    The guard cells become turgid.

    The outer wall of guard cell is thinnerand more elastic than the thicker

    inner wall.

    There are cellulose microfibrils

    radially arranged around the cell wall

    and the ends of the two guard cellsare joined.

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    The increased turgor pressure

    therefore causes the guard cells

    to curve outward and the stomaopens.

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    Stomatal closure

    Stomatal closure can be triggered by

    water stress, high temperature,

    increasing CO levels in the leaf

    mesophyll and low light intensity (night-

    time).

    The hormone abscisic acid (ABA) issecreted by plant cell when transpiration

    rate is high and soil water is low.

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    This often occurs around mid-day or

    when a plant begins to wilt. The ABA binds the receptors at the

    cell membrane of the guard cells.

    This increases the permeability of

    calcium channels in the cell

    membrane.

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    Calcium ions (Ca ) enter into the

    guard cell. The influx of Ca also triggers the

    release of Ca from the cell vacuole

    into the cytosol.

    The accumulation of Ca inhibits

    the proton pump.

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    Potassium ions (K) move out of

    the guard cells into subsidiarycells.

    In some plants, Cl and certainorganic ions e.g malate ions also

    move out of the guard cells.

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    In the older hypothesis, at night

    glucose is converted to insolublestarch.

    The water potential in the guard cell

    increases.

    Water diffuses out to neighbouring

    subsidiary cells by osmosis.

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    The turgor pressure in the guard cells

    decreases, the cells become flaccidand the stoma closes.

    At the night, the chloroplasts in the

    guard cells do not photosynthesise.

    Less ATP is produced.

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    There is no active uptake of K

    ions.

    Instead, the K ions diffuse out of

    the guard cells.

    The cells become flaccid and the

    stoma closes.

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    Factors affecting the opening and

    closing of stoma

    Circadian rhythms

    Some plants have biological clock whereby

    stomata open during the day and close at

    night

    Plants were observed to continue opening

    and closing their stoma even when exposed

    to 24 hours of continuous light

    However, some plants in hot dry conditions

    (CAM plants) have an opposite rhythm

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    Water balance

    When there is a shortage of water,

    plants will wilt. Wilting closes stomata

    Concentration of CO

    A low concentration of CO in thesurrounding air causes stomata to open

    Light

    Light causes stomata to open wherebyplants are more sensitive to blue light

    compared to red light