G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4
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DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM EXPOSURE TO FIRE COMBUSTION PRODUCTS G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4 1 School of Civil and Environmental Engineering 2 School of Chemical sciences 3 School of Chemical Engineering and Industrial Chemistry 4 School of Safety Science The University of New South Wales, Sydney 2052, Australia
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DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM EXPOSURE TO FIRE COMBUSTION PRODUCTS. G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4 1 School of Civil and Environmental Engineering 2 School of Chemical sciences - PowerPoint PPT Presentation
Transcript of G. Chattopadhyay 1 , Paul Hallahan 2 , Paul Brockbank 3 , F. Lestari 4 , A. Hayes 4 , A. Green 4
DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM
EXPOSURE TO FIRE COMBUSTION PRODUCTS
G. Chattopadhyay1, Paul Hallahan2, Paul Brockbank3, F. Lestari4, A. Hayes4, A. Green4
1School of Civil and Environmental Engineering2School of Chemical sciences
3School of Chemical Engineering and Industrial Chemistry4School of Safety Science
The University of New South Wales, Sydney 2052, Australia
TECHNIQUES INCORPORATED IN THE APPARATUS
• Fire combustion dynamics measurement (TGA analysis)
• In vitro cytotoxicity measurement – Impinger– Direct exposure
• Sampling for chemical analysis
• GC-MS Chemical analysis– Thermal desorption– Cryo-focusing
TGA data Acc
Flow control
Load cell for TGA data
Tube furnace
Mixing chamber
Basic components of the apparatus
Frame
Temperature profile inside tube furnace at 680oC furnace setting
0
100
200
300
400
500
600
700
800
0 10 20 30 40
Distance from the bottom (cm)
Tem
per
atu
re (
oC
)
Tube Furnace
Quartz tube 1000 mm long X 40 mm diameter
Load Cell for TGA data
TGA data cable
Gas inlet
Bottom of the quartz Tube furnace
Ground glass joint Fits onto the tubefurnace
Hole in polycarbonateEnclosure to tarebalance
Polycarbonate enclosure
Mixing Chamber
Sampling lines
Exhaust line from mixing chamberto lab suction system
Exhaust line to lab suction system fromTube furnace enclosure
Mixing Chamber : 310 (L) X 300(W) X
310 (H)
Volume: 28.83 (L)
Flow control components
Temperature control forDirect exposure cells
Flow control for gas and sampling streams
Impinger
Suction pump for Collecting samples
Temperature control For tube furnace
Air sampling using culture medium (serum free)
96 well platesMTS assay
Experiment flowchart
Impinger method
inletoutlet
membrane
Membrane preparationSnapwell insert
Navicyte chamber MTS Assay
Direct exposure at the air/liquid interface
Chemical analysis for combustion products
Sorption in a solid sorbent
ATD-GCMS
Tube DesorbTube Desorb
Cold trap at -20° CCold trap at -20° C
To GC column transfer lineTo GC column transfer line
Trap DesorbTrap Desorb
To GC column transfer lineTo GC column transfer line
PMMA Mass Loss Data (Non-Flaming)
0
2
4
6
8
10
12
14
16
0 500 1000 1500 2000
Time (second)
weig
ht
(gra
m)
Preliminary result CO2
Impinger methodDirect exposure
0
20
40
60
80
100
120
0 20 40 60 80 100
Dose %
% C
ell v
iabi
lity
Air
A549
HepG2
Fibroblast
0%
20%
40%
60%
80%
100%
120%
Control CO2
% C
ell v
iabi
lity
A549
HepG2
Fibroblast
4h post exposure incubation time
0
20
40
60
80
100
120
140
0 20 40 60 80 100
Dose (%)
%ce
ll vi
abili
ty
A549
Fibro
HepG2
24 h postexposure incubation time
0
20
40
60
80
100
120
0 20 40 60 80 100
Dose (%)
% c
ell v
iabi
lity
A549
Skin Fibroblast
HepG2
PMMA cytotoxicity under non-flaming condition at different post exposure time
Potential applicationsPotential applications
• Fire toxicity testing for materials or products
• Screening and early sign of fire hazard to fire fighters (biosensor)
• Toxicity studies for gases, particulates, non-hydrosoluble compounds, complex mixtures, and organic volatile compounds
• Many applications in odour analysis and odour management
