Fridge -4 o C Pilot test 12 15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C...

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Fridge -4 o C Pilot test 12 – 15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans each One pack per treatment 11 Nov 07 Each pack undergone their respective treatment 24 hours, started12pm 12 Nov 07 12pm

description

Preparation of Rice water Made a mark on the scoop (around 15mL of water) 10 grams of rice poured into a scoop. 10 g Rice 10 g Rice 1 min

Transcript of Fridge -4 o C Pilot test 12 15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C...

Page 1: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Fridge-4oC

Pilot test 12 – 15 Nov 07

Incubator37oC

Incubator32oC

Room temperature23oC

Purchased at Chinese Medicine Store

Packs of 20 beans each

One pack per treatment

11 Nov 07

Each pack undergone their respective treatment

24 hours, started12pm

12 Nov 07

12pm

Page 2: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Fold into two and roll up and place along side of the cup

200mL

6cm

6.5cm

4.3cm

20 beans below the first line on the cup

200mL

6cm

6.5cm

4.3cm

Pilot test

Page 3: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Pilot test

Preparation of Rice water

Made a mark on the scoop (around 15mL of water)

10 grams of rice poured into a scoop.

10 g Rice1 min

Page 4: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Pilot test

Watering of mung beans

Page 5: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Pilot test

Room

Window

Desk

light

drawers

door

Placement of set-ups

Page 6: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Total 12 treatments with 1 set up each = Total 12 set ups

Randomised arrangement

Grown in closed drawer darkness,homogenised environment (same temperature, humidity)

Watered at around 6pm everyday (13-16 Nov 07) (sun already set hence no effect from natural light from window)

Drawer was opened for around 45 minutes for watering.

With certain light exposure (turning on of the lights in the room)

Pilot test

Ctrl-4oC

Red-4oC

White32oC

RedRT

CtrlRT

Red37oC

White37oC

Red32oC

Ctrl32oC

Ctrl37oC

WhiteRT

White-4oC

Placement of set-ups

Page 7: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Actual test (17 – 21 Nov 07) 5 days

Watered at around 6pm everyday for 5 days

Ctrl-4oC

Ctrl-4oC

Ctrl-4oC

Ctrl-4oC

Ctrl-4oC

Ctrl-4oC

Red-4oC

Red-4oC

Red-4oC

White32oC

White32oC

White32oC

RedRT

RedRT

RedRT

CtrlRT

CtrlRT

CtrlRT

WhiteRT

WhiteRT

WhiteRT

Red32oC

Red32oC

Red32oC

Ctrl32oC

Ctrl32oC

Ctrl32oC

White-4oC

White-4oC

White-4oC

Total 9 treatments with 3 replicates each = Total 27 set ups

Rice type / Temperature (oC) White Red Control (no rice)

-4 White/-4 Red/-4 Ctrl/-4

23 (Room temperature) White/RT Red/RT Ctrl/RT

32 White/32 Red/32 Ctrl/32

Page 8: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Watering process

• Room light off, Desk light on

• Small amount of light used during watering of plants

• Each drawer was opened for around 30mins

Actual test (17 – 21 Nov 07) 5 days

6pm (after sunset)Window

Desk

light

drawers

door

chai

rch

air

Page 9: Fridge -4 o C Pilot test 12  15 Nov 07 Incubator 37 o C Incubator 32 o C Room temperature 23 o C Purchased at Chinese Medicine Store Packs of 20 beans.

Data collection

• One time point• Using normal ruler, we

measure the length of the shoot and root to an accuracy of 1 d.p.

shoot

root