GENE CLONIN GAND DNA ANALYSI SAn Introductio n

T.A . BROWN

Fourth Edition

Preface to the Fourth Edition, vi i

Preface to the Third Edition, ix

Preface to the Second Edition, x

Preface to the First Edition, xii

Part 1 : The Basic Principles of Gene Clonin gand DNA Analysis, 1

1 Why Gene Cloning and DNA Analysis are Important, 3

2 Vehicles for Gene Cloning : Plasmids and Bacteriophages, 1 4

3 Purification of DNA from Living Cells, 28

4 Manipulation of Purified DNA, 5 2

5 Introduction of DNA into Living Cells, 8 5

6 Cloning Vectors for E. coli, 104

7 Cloning Vectors for Eukaryotes, 13 0

8 How to Obtain a Clone of a Specific Gene, 156

9 The Polymerase Chain Reaction, 17 9

Part 2: The Applications of Gene Cloning an dDNA Analysis in Research, 195

10 Studying Gene Location and Structure, 19 7

11 Studying Gene Expression and Function, 21 7

12 Studying Genomes, 246

Part 3: The Applications of Gene Cloning an dDNA Analysis in Biotechnology, 269

13 Production of Protein from Cloned Genes, 271

14 Gene Cloning and DNA Analysis in Medicine, 293

15 Gene Cloning and DNA Analysis in Agriculture, 31 3

16 Gene Cloning and DNA Analysis in Forensic Science, 32 9

Glossary, 34 1

Index, 357