FOOD DIAGNOSTICS

Drug Residue Testing for the Dairy Industry

Cost effective milk screening solution

Randox Food Diagnostics offer excellent tools for screening of antimicrobials and growth hormones in milk. Excellent limits of detection and simple sample preparations can be obtained using either ELISA screening or the unique multi-analyte screening, our patented Biochip Array Technology.

Randox presents the world’s only multi-analyte quantitative Drug Residue analyser, the Evidence Investigator. The use of the Evidence Investigator allows simultaneous detection of multiple antibiotics from a single sample. The system utilises Randox patented Biochip Array Technology. Generally the milk sample will only require a simple dilution prior to application, enhancing the already user-friendly protocol.This unique multi-analyte testing platform will reduce labour costs, increase throughput and guarantee overall productivity improvement.

ELISA

Antibiotic screening tests available:Biochip Multi-analyte Screening

Randox Food Diagnostics milk testing

AvermectinBeta-AgonistChloramphenicolClenbuterolFlumequineRactopamineStreptomycinSulphamethoxazoleSulphaquinoxalineSulphadiazineSulphamethazineBeta-LactamQuinolonesNitrofuran-AOZ-AMOZ-AHD-SEM

Antimicrobial Array 1 Plus (EV3775)SulphadimethoxineSulphadiazineSulphadoxineSulphamethizoleSulphachlorpyridazineSulphamethoxypyridazineSulphamerazineSulphisoxazoleSulphathiazoleSulphamethazineSulphaquinoxalineSulphapyridineSulphamethoxazoleSulphamonomethoxineTrimethoprim

Anthelmintics Array(EV3770)Amino BenzimidazolesAvermectins (generic)BenzimidazolesLevamisoleMoxidectinThiabendazoleTriclabendazole

Antimicrobial Array II(EV3524A)QuinolonesCeftiofurThiamphenicolStreptomycinTylosinTetracyclines

Antimicrobial Array III(EV3695)AOZAMOZAHDSEMChloramphenicol

Antimicrobial Array III CAP only (EV3738)ChloramphenicolChloramphenicol Glucuronide

Beta-Lactam ArrayPenicillinsAmoxicillinAmpicillinCloxacillinDicloxacillinNafcillinOxacillinPenethamatePenicillin G

CephalosporinsCefacetrilCefazolinCefoperazoneCefquinomeCeftiofurCefuroximeCephalexinCephaloniumCephapirin

Cost Effective Antibiotic Milk Screening

‘Will reduce costs, increase throughput and guarantee overall productivity improvement’

ELISA kits available for milk testing

ELISA Tests for milk testing

Randox Food Diagnostics ELISA kits allow screening of drug residues in various matrices. Randox ELISA kits show favourable comparison with confirmatory methods.

Randox ELISA Kit Benefits:• Easy to use, highly specific, cost effective screening method

• Intra and inter assay precision typically <10% CV

• Fast – up to 40 samples can be analysed in 90 minutes

• Lower set-up costs as no expensive equipment required

• Randox ELISA tests can be fully automated

• Simple sample preparation - generally milk sample will

only require simple dilution

• Excellent generic antibodies

• Quinolones - detects 17 analytes

• β - Lactams - detects 11 analytes

Cost Effective Antibiotic Milk Screening

Drug Class Cat No LOD (ppb)

βeta-Agonist SU2148 0.2βeta-Lactam BL3448 0.38Chloramphenicol CN1469 0.1Clenbuterol CB1418 0.1Quinolones QL3454 0.25Ractopamine RT3451 0.07Sulphadiazine SZ2147 5Sulphamethazine SM2146 5Sulphaquinoxaline SQ2145 5Streptomycin STP3468 3

Drug Class Cat No LOD (ppb)

βeta-Agonist SU2148 0.2βeta-Lactam BL3448 0.15Chloramphenicol CN1469 0.08Clenbuterol CB1418 0.1Ractopamine RT3451 0.15

ELISA kits available for milk powder

Centrifuge

Apply Ractopamine

Clenbuterol

Chloramphenicol

Beta Lactam

Beta-Agonist

40 samples/200 tests

One step sample preparation

Simple and rapid milk preparation using the Randox antimicrobial arrays

1. Centrifuge milk sample at 2880rcf for 10 minutes and dilute if required

2. Apply buffers/calibrators/ samples/controls to the applicable biochip wells

3. Incubate for 30minutes using our customised thermoshaker

4. Apply detection labelled conjugate to the applicable biochip wells

5. Incubate for 60 minutes using our customised thermoshaker

6. Perform six wash steps on each biochip to remove any unbound material

7. Apply chemiluminescent substrate to each biochip well

8. Measure light output using the Evidence Investigator analyser to obtain results

Cost Effective Antibiotic Milk Screening

Ideal for milk drug residue laboratories• Multi-analyte screening for antibiotic residues

• Consolidation of testing to provide multiple test results from a single sample

• Same sample preparation method can be applied across a number of arrays

• Simple sample preparations - minimum technical expertise required.

• Excellent generic nature of antibodies allows detection of 50 different

antimicrobials from a single sample

Multi-analyte screening for veterinary drug residues. The ultimate drug residue screening platform

Key Benefits• Cost savings

• High Quality

• Minimal Consumable Costs

• Less Labour

• Faster Turnaround time

• Higher Throughput

• 50 Target Screens

• Simple Protocols

• >65 New Tests in Development

• Consolidation of Tests on the same platform

Biochip multi-analyte antibiotic milk screening

Specificity/ Limits of Detection (LOD)

Biochip multi-analyte antibiotic milk screening

Cost Effective Antibiotic Milk Screening

Assay Compound Specificity (%CR)

AOZ 4-NP-AOZFurazolidone

1008

AMOZ 4-NP-AMOZFuraltadone

10041

AHD 4-NP-AHDNitrofurantoin

10042

SEM 4-NP-SEM5-Nitro-2-furaldehydeSemicarbazone

100

14

Chloramphenicol ChloramphenicolChloramphenicol Glucuronide

10075.1

08/102/296, 09/121/296CR= Cross Reactivity*Milk preparation for nitrofurans currently indevelopment

Antimicrobial III Array (EV3695)

08/367,377,382,387/295/11/059/490-10

Assay Compound Specificity (%CR)

LOD(ppb)

Quinolones NorfloxacinPefloxacin Enrofloxacin CiprofloxacinOfloxacin EnoxacinPipemidic acid FleroxacinLevofloxacinNadifloxacinOrbifloxacinDanofloxacinMarbofloxacinOxolinic acidDifloxacinPazufloxacin Sarafloxacin

100 84 76595754363232 2723 20161287 6

1

Ceftiofur Ceftiofur 100 1.5

Thiamphenicol FlorphenicolThiamphenicol

10053

0.5

Streptomycin Streptomycin Dihydrostreptomycin

100 182

2

Tylosin TylosinTilmicosin

10037

2.5

Tetracyclines Tetracycline 4-epitetracyclineRolitetracycline 4-epioxytetracycline OxytetracyclineChlortetracycline Demeclocycline Doxycycline4-epichlortetracyclineMethacycline

100 876752525141232011

1

Antimicrobial II Array (EV3524A)

CR = Cross Reactivity

Assay Compound Specificity (%CR)

LOD(ppb)

Sulphadimethoxine Sulphadimethoxine 100 0.6

Sulphadiazine Sulphadiazine 100 0.5

Sulphadoxine Sulphadoxine 100 0.5

Sulphamethizole SulphamethizoleSulphachlorpyridazine

10013.3

0.5

Sulphachlorpyridazine Sulphachlorpyridazine 100 0.5

Sulphamethoxypyridazine SulphamethoxypyridazineSulphaethoxypyridazine

10056

0.5

Sulphamerazine Sulphamerazine 100 0.5

Sulphisoxazole Sulphisoxazole 100 0.5

Sulphathiazole SulphathiazoleSulphadiazine

1006.2

0.5

Sulphamethazine Sulphamethazine 100 2.5

Sulphaquinoxaline Sulphaquinoxaline 100 0.5

Sulphapyridine SulphapyridineSulphasalazine

10012.9

0.5

Sulphamethoxazole Sulphamethoxazole SulphamethizoleSulphachlorpyridazine

10092

12.1

0.5

Sulphamonomethoxine Sulphamonomethoxine 100 2.0

Trimethoprim Trimethoprim 100 0.5

Antimicrobial Array 1 Plus (EV3775)

05/192/SP; 07/578/SF; 09.1026.346

Assay Compound Specificity (%CR)

LOD (ppb)

Chloramphenicol ChloramphenicolChloramphenicol Glucuronide

10075.1

0.04

Antimicrobial III Array Chloramphenicol Only Array (EV3738)

11/046/047-490-08

Cost Effective Antibiotic Milk Screening

Biochip Multi-analyte Antibiotic Milk Screening

Specificity (%CR)

LOD(ppb)

Benzimidazoles (BZS) 0.2Albendazole 2-amino sulphone 141

Amino-flubendazole 99

Amino-mebendazole 141

Avermectins 0.7Ivermectin 100

Abamectin 178

Doramectin 75

Emamectin Benzoate 254

Eprinomectin 191

Benzimidazoles 0.2Albendazole 100

Albendazole sulphoxide 99

Albendazole sulphone 178

Fenbendazole 10

Oxfendazole (Fenbendazole sulphoxide) 40

Oxibendazole 48

Mebendazole 18

Oxfendazole sulphone (Fenbendazole sulphone) 14

Flubendazole 29

Hydroxy Flubendazole 2

Hydroxy Mebendazole 1

Anthelmintics Array (EV3770)

Specificity (%CR)

LOD(ppb)

Levamisole 3.0Levamisole 100

Moxidectin 3.0Moxidectin 100

Thiabendazole 0.25Thiabendazole 100

5-hydroxythiabendazole 91

Cambendazole 800

Triclabendazole 0.8Triclabendazole (TCBZ) 100

Triclabendazole Sulphoxide 40

Keto-triclabendazole 150

Triclabendazole Sulphone 1

Beta Lactam Array (EV3793)Antibiotic Concentration

(ng/ml) resulting in ≥95% positive

test results

Ampicillin 0.8

Amoxicillin 0.8

Cloxacillin 3

Dicloxacillin 3

Nafcillin 18

Oxacillin 3

Penicillin G 0.4

Penicillin V 0.4

Antibiotic Concentration (ng/ml) resulting in ≥95% positive

test results

Cefacetril 12.5

Cefazolin 25

Cefoperazone 5

Cefquinome 4

Ceftiofur 20

Cephalexin 100

Cephalonium 2

Cephapirin 6

Limits of detection(ppb)

Beta-Lactams (BLACT) 0.6

‘Excellent limits of detection with simple sample preparation’

ANTHELMINTICS ARRAy (EV3770)Amino BenzimidazolesAvermectins (generic)Benzimidazoles LevamisoleMoxidectinThiabendazoleTriclabendazole

ANTIMICROBIAL ARRAy I PLUS (EV3775)SulphachlorpyridazineSulphadiazineSulphadimethoxineSulphadoxineSulphamerazineSulphamethazineSulphamethizoleSulphamethoxazoleSulphamethoxypyridazineSulphamonomethoxineSulphapyridineSulphaquinoxaline SulphathiazoleSulphisoxazoleTrimethoprim

ANTIMICROBIAL ARRAy II (EV3524A)CeftiofurFlorphenicol/ Thiamphenicol Quinolones (generic)Streptomycin/DihydrostreptomycinTetracyclines (generic)Tylosin/ Tilmicosin

ANTIMICROBIAL ARRAy III (EV3695)AHDAMOZAOZSEMChloramphenicolChloramphenicol Glucuronide

ANTIMICROBIAL ARRAy IIICHLORAMPHENICOL ONLy (EV3738)ChloramphenicolChloramphenicol Glucuronide

Multi-analyte screening for

Veterinary Drug Residues

The ultimate cost saving

Drug Residue Screening solution

GROwTH PROMOTER RAPID URINE SCREEN (EV3521)β -AgonistsBoldenoneCorticosteroids RactopamineStanozolol Trenbolone Zeranol

GROwTH PROMOTER MULTIPLE MATRIx SCREEN (EV3726)β - AgonistsBoldenoneCorticosteroidsNandroloneRactopamineStanozololStilbenesTrenbolone Zeranol

SyNTHETIC STEROIDS ARRAy (EV3694)17β - ClostebolEthinylestradiolGestagens (generic)Methyltestosterone

MATRIZ PARA BETA LACTÁMICOS (EV3793)PenicilinasAmoxicilinaAmpicilinaCloxacilinaDicloxacilinaNafcilinaOxacilinaPenetamatePenicilina G

CefalosporinasCefacetrilCefazolinaCefoperazonaCefquinomaCeftiofurCefuroximaCefalexinaCefaloniumCefapirina

ANTIMICROBIAL ARRAYS GROWTH PROMOTERS ARRAYS

MATRIZ PARA BETA LACTÁMICOS

Matriz para Antimicrobianos IVMatriz para β-agonistasMatriz para CoccidiostáticosMatriz para Nitroimidazoles

PRÓXIMOS LANZAMIENTOS

LT31

1RFD

OCT

12

Sulphonamides

Chloramphenicol

Tetracyclines

Nitrofurans

Aminoglycosides

Macrolides

Quinolones

Beta Lactams

Anthelmintics

Gestagens/Androgens/

Corticosteroids

Beta Agonists

Clenbuterol

Ractopamine

Boldenone

Stanozolol

Trenbolone

Nandrolone

Zeranol

Screening test menu includes

Estrogens

FOOD DIAGNOSTICS

Randox Food Diagnostics provides a unique multi-analyte testing platform that will improve the speed of throughput of your food testing laboratory.

• Reduce labour costs• Fast sample turnaround time• Consolidates testing• Simple sample preparation• Quality reassurance• Overall productivity improvements

Multi-analyte Testing Benefits:

Matrices available:• Tissue• Honey• Feed

• Urine• Milk

Tied up doing things the old fashioned way?Switch to a more efficient method

Information correct at time of print. All Randox Food Diagnostics products are made in the UK. Randox Food Diagnostics is a company registered within Northern Ireland with Company Number NI069074. VAT Number GB 151 6827 08. Product availability may vary from country to country. Please contact your local Randox Food Diagnostics representative for information.

Randox Food Diagnostics, 30 Cherryvalley Road, Crumlin, County Antrim, BT29 4QN, United Kingdomt +44 (0) 28 9442 2413 f +44 (0) 28 9445 2912 e enquiries@randoxfooddiagnostics.com I www.randoxfooddiagnostics.com

Distributor: Bentley Instruments, ZA Brunehaut, 840 rue Curie, 62161 Maroeuil-France Contact: bentleyeurope@orange.fr