Foliar Nutrient Analysis Foliage collection and interpretation of laboratory results.
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Transcript of Foliar Nutrient Analysis Foliage collection and interpretation of laboratory results.
Foliar Nutrient AnalysisFoliage collection and interpretation of laboratory results
Operational fertilization decision-making
Forest level considerations
Stand level considerations operational factors biological factors
species stand structure crown conditions insect/disease nutrient status
Foliar analysis as a planning tool
Foliar analysis can be used to: confirm N deficiency
Foliar analysis as a planning tool
Foliar analysis can be used to: confirm N deficiency identify secondary nutrient deficiencies (e.g., S, B)
Foliar analysis as a planning tool
Foliar analysis can be used to: confirm N deficiency identify secondary nutrient deficiencies (e.g., S, B) make appropriate fertilizer prescriptions
Foliar analysis as a planning tool
Foliar analysis can be used to: confirm N deficiency identify secondary nutrient deficiencies (e.g., S, B) make appropriate fertilizer prescriptions assess post-fertilization nutrient uptake and foliar
nutrient balance
How much foliar sampling is needed?
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
Strategically allocate foliar sampling expenditures
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
Strategically allocate foliar sampling expenditures Utilize foliar nutrient data and/or fertilization
growth response results from similar nearby stands
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
Strategically allocate foliar sampling expenditures Utilize foliar nutrient data and/or fertilization growth response
results from similar nearby stands Stratify candidate blocks into homogeneous
combinations (species, age, BEC, stand history, stand conditions)
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
Strategically allocate foliar sampling expenditures Utilize foliar nutrient data and/or fertilization growth response
results from similar nearby stands Stratify candidate blocks into homogeneous combinations
(species, age, BEC, stand history, stand conditions) Collect representative composite foliage
samples from each major combination
How much foliar sampling is needed?
Foliar sampling should only be undertaken on candidate sites that satisfy other forest- and stand-level selection criteria
Strategically allocate foliar sampling expenditures Utilize foliar nutrient data and/or fertilization growth response
results from similar nearby stands Stratify candidate blocks into homogeneous combinations
(species, age, BEC, stand history, stand conditions) Collect representative composite foliage samples from each
major combination Operational fertilization projects comprised of a
small number of large and uniform blocks will require a relatively small amount of foliar sampling
Factors affecting interpretation of foliar nutrient data
Factors affecting interpretation of foliar nutrient data
Foliar sampling protocol
Foliar Sampling Protocol
Foliar Sampling Protocol
Sample during the dormant season
Seasonal change in foliar %N in Douglas-fir foliage
0.6
0.8
1
1.2
1.4
1.6
1.8
Fo
liar
N (
%)
May June July Aug Sept Oct Nov Dec
Moderate deficiency
Month
Sampling period
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Collect foliage from between top 1/4 and bottom 1/2 of live crown
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Collect foliage from between top 1/4 and bottom 1/2 of live crown
Collect foliage from healthy, representative trees
Do not collect foliage fromunhealthy trees!!!
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Collect foliage from between top 1/4 and bottom 1/2 of live crown
Collect foliage from representative trees
Collect foliage from at least 20 trees per stand or stratum
Foliar sampling layout
x
x
x
x
x
x
xx
xx
xx
xx
xx
xx
xx
RoadL
ine
1
Line 2
/02
/01
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Collect foliage from between top 1/4 and bottom 1/2 of live crown
Collect foliage from representative trees
Collect foliage from at least 20 trees per stand or stratum
For routine diagnoses, combine equal amounts of foliage from individual trees into one composite sample per stratum
Foliar Sampling Protocol
Sample during the dormant season
Sample current year’s foliage
Collect foliage from between top 1/4 and bottom 1/2 of live crown
Collect foliage from representative trees
Collect foliage from at least 20 trees per stand or stratum
For routine diagnoses, combine equal amounts of foliage from individual trees into one composite sample per stratum
Keep samples cool until foliage is dried
Factors affecting interpretation of foliar nutrient data
Foliar sampling protocol
Site ecological characteristics
from DeLong (2003)
from DeLong (2003)
Factors affecting interpretation of foliar nutrient data
Foliar sampling protocol
Site ecological characteristics
Laboratory analytical methodology
Relationship between foliar N analytical methodologiesdry combustion vs. wet digestion
0.9 1.0 1.1 1.2 1.3 1.4 1.50.8
0.9
1.0
1.1
1.2
1.3
1.4
% N (dry combustion)
% N
(w
et
dig
es
tio
n)
Relationship between foliar S analytical methodologies dry combustion vs. wet digestion
0.06 0.07 0.08 0.09 0.100.05
0.06
0.07
0.08
0.09
% S (dry combustion)
% S
(w
et
dig
es
tio
n)
Accounting for differences in laboratory analytical methodology
Differences may be large enough to affect interpretation
Accounting for differences in laboratory analytical methodology
Differences may be large enough to affect interpretation
Nutrient interpretative criteria do not account for differences in methodology
Accounting for differences in laboratory analytical methodology
Differences may be large enough to affect interpretation
Nutrient interpretative criteria do not account for differences in methodology
Known differences in laboratory analytical results can be used to “normalize” foliar data prior to interpretation
Accounting for differences in laboratory analytical methodology
Differences may be large enough to affect interpretation
Nutrient interpretative criteria do not account for differences in methodology
Known differences in laboratory analytical results can be used to “normalize” foliar data prior to interpretation
“Normalization” requires inter-laboratory comparisons
Accounting for differences in laboratory analytical methodology
Differences may be large enough to affect interpretation
Nutrient interpretative criteria do not account for differences in methodology
Known differences in laboratory analytical results can be used to “normalize” foliar data prior to interpretation
“Normalization” requires inter-laboratory comparisons
The “normalization” process does not make inferences about the quality of foliar nutrient data
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously collected Pl foliage samples were selected
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Each sample was thoroughly mixed and split into two sub-samples
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Each sample was thoroughly mixed and split into two sub-samples
One sub-sample was shipped to each lab in December 2012
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Each sample was thoroughly mixed and split into two sub-samples
One sub-sample was shipped to each lab in December 2012
For each nutrient, laboratory results were reviewed and subjected to regression analysis
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Each sample was thoroughly mixed and split into two sub-samples
One sub-sample was shipped to each lab in December 2012
For each nutrient, laboratory results were reviewed and subjected to regression analysis
Based on previous research, equations were selected to “normalize” foliar nutrient data
Inter-laboratory comparisonPacific Soil Analysis vs. Ministry of Environment
50 previously analyzed foliage samples were used
Samples were selected to cover a broad range of species and foliar nutrient levels
Each sample was thoroughly mixed and split into two sub-samples
One sub-sample was shipped to each lab in December 2012
For each nutrient, laboratory results were reviewed and subjected to regression analysis
Based on previous research, equations were selected to “normalize” foliar nutrient data
An Excel spreadsheet was developed to facilitate “normalization” for practitioners
Laboratory foliar N comparison PSAI vs. MoE
0.9 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2.00.80.91.01.11.21.31.41.51.61.71.81.9
% Nitrogen (MoE)
% N
itro
ge
n (
PS
AI)
y = 0.9584x R2 = 0.959
Laboratory foliar S comparison PSAI vs. MoE
0.08 0.10 0.12 0.14 0.16 0.18 0.200.070.080.090.100.110.120.130.140.150.160.17
% Sulphur (MoE)
% S
ulp
hu
r (P
SA
I)
y = -0.6267x2 + 0.9558xR2 = 0.888
Laboratory foliar SO4-S comparison PSAI vs. MoE
0 100 200 300 400 5000
100
200
300
400
500
600
ppm Sulphate-S (MoE)
pp
m S
ulp
ha
te-S
(P
SA
I)
y = -0.0008x2 + 1.4164xR2 = 0.884
Laboratory foliar P comparison PSAI vs. MoE
0.10 0.15 0.20 0.25 0.30 0.350.10
0.15
0.20
0.25
0.30
0.35
f(x) = 0.949246219750039 xR² = 0.998330009127891
% Phosphorus (MoE)
% P
ho
sp
ho
rus
(P
SA
I)
Laboratory foliar K comparison PSAI vs. MoE
0.10 0.15 0.20 0.25 0.30 0.350.10
0.15
0.20
0.25
0.30
0.35
f(x) = 0.949246219750039 xR² = 0.998330009127891
% Potassium (MoE)
% P
ota
ss
ium
(P
SA
I)
Laboratory foliar Ca comparison PSAI vs. MoE
0.00 0.20 0.40 0.60 0.80 1.00 1.200.00
0.20
0.40
0.60
0.80
1.00
1.20f(x) = 0.359162995512975 x² + 0.734591772839139 xR² = 0.996003359942529
% Calcium (MoE)
% C
alc
ium
(P
SA
I)
Laboratory foliar Mg comparison PSAI vs. MoE
0.06 0.08 0.10 0.12 0.14 0.16 0.18 0.20 0.22 0.240.06
0.08
0.10
0.12
0.14
0.16
0.18
0.20
0.22
0.24f(x) = 1.02490310339437 xR² = 0.99859621254757
% Magnesium (MoE)
% M
ag
ne
siu
m (
PS
AI)
Laboratory foliar B comparison PSAI vs. MoE
0 10 20 30 40 50 60 70 800
10
20
30
40
50
60
f(x) = − 0.0012318676908454 x² + 0.873218070017739 xR² = 0.99735810044827
ppm Boron (MoE)
pp
m B
oro
n (
PS
AI)
Factors affecting interpretation of foliar nutrient data
Foliar sampling protocol
Site ecological characteristics
Laboratory analytical methodology
Availability of reliable foliar nutrient interpretative criteria
Relationship between tree growth and foliar nutrient concentration
Foliar nutrient concentration
Gro
wth
Deficient
Critical Adequate
Toxic
from Carter (1992)
from Brockley (2001)
Revised foliar nutrient interpretative criteria
Revised foliar nutrient interpretative criteria
Information sources: Previously published interpretative criteria
Revised foliar nutrient interpretative criteria
Information sources: Previously published interpretative criteria
Published foliar nutrient and growth response data from Pinus, Picea and Pseudotsuga fertilization studies
Revised foliar nutrient interpretative criteria
Information sources: Previously published interpretative criteria
Published foliar nutrient and growth response data from Pinus, Picea and Pseudotsuga fertilization studies
Unpublished foliar nutrient and growth response data from BC fertilization studies
Revised foliar nutrient interpretative criteria Macronutrients
Foliar concentration (% dry weight)
Element Interpretation Pl Sx Fd N Severely deficient < 1.00 < 0.90 < 1.00 Mod. to severely deficient 1.00 – 1.15 0.90 – 1.10 1.00 – 1.15 Slightly to mod. deficient 1.15 – 1.30 1.10 – 1.30 1.15 – 1.30 Adequate > 1.30 > 1.30 > 1.30 P Severely deficient < 0.08 < 0.10 < 0.11 Mod. to severely deficient 0.08 – 0.10 0.11 – 0.12 0.11 – 0.13 Slightly to mod. deficient 0.10 – 0.12 0.12 – 0.14 0.13 – 0.15 Adequate > 0.12 > 0.14 > 0.15 K Severely deficient < 0.30 < 0.40 < 0.45 Mod. to severely deficient 0.30 – 0.35 0.40 – 0.45 0.45 – 0.55 Slightly to mod. deficient 0.35 – 0.40 0.45 – 0.50 0.55 – 0.60 Adequate > 0.40 > 0.50 > 0.60
Revised March 2012
Revised foliar nutrient interpretative criteria Macronutrients cont’d
Foliar concentration (% dry weight)
Element Interpretation Pl Sx Fd Ca Severely deficient < 0.06 < 0.10 < 0.10 Mod. to severely deficient 0.06 – 0.08 0.10 – 0.15 0.10 – 0.15 Slightly to mod. deficient 0.08 – 0.10 0.15 – 0.20 0.15 – 0.20 Adequate > 0.10 > 0.20 > 0.20 Mg Severely deficient < 0.04 < 0.04 < 0.06 Mod. to severely deficient 0.04 – 0.06 0.04 – 0.06 0.06 – 0.08 Slightly to mod. deficient 0.06 – 0.08 0.06 – 0.08 0.08 – 0.10 Adequate > 0.08 > 0.08 > 0.10 S Severely deficient < 0.06 < 0.06 < 0.06 Mod. to severely deficient 0.06 – 0.08 0.06 – 0.08 0.06 – 0.08 Slightly to mod. deficient 0.08 – 0.10 0.08 – 0.10 0.08 – 0.10 Adequate > 0.10 > 0.10 > 0.10
Revised March 2012
Revised foliar nutrient interpretative criteria Sulphate-S and Boron
Foliar concentration (ppm dry weight)
Element Interpretation Pl Sx Fd SO4-S Severely deficient < 40 < 60 < 100 Mod. to severely deficient 40 – 60 60 – 80 100 – 150 Slightly to mod. deficient 60 – 80 80 – 100 150 – 200 Adequate > 80 > 100 > 200 B Severely deficient < 3 < 3 < 3 Probable deficiency 3 – 6 3 – 6 3 – 6 Possible deficiency 6 – 12 6 – 12 6 – 12 No deficiency > 12 > 12 > 12
Revised March 2012
Revised foliar nutrient interpretative criteria Nutrient ratios
Nutrient ratio
Element Interpretation Pl Sx Fd
N:P Moderate to severe P deficiency > 13 > 11 > 11 Slight to moderate P deficiency 11 – 13 10 – 11 10 – 11 Possible slight P deficiency 10 – 11 9 – 10 9 – 10 No P deficiency < 10 < 9 < 9 N:K Moderate to severe K deficiency > 4.5 > 4.0 > 3.5 Slight to moderate K deficiency 3.5 – 4.5 3.0 – 4.0 2.5 – 3.5 Possible slight K deficiency 2.5 – 3.5 2.0 – 3.0 2.0 – 2.5 No K deficiency < 2.5 < 2.0 < 2.0 N:S Severe S deficiency > 25 > 25 > 25 Moderate to severe S deficiency 20 – 25 20 – 25 20 – 25 Slight to moderate S deficiency 15 – 20 15 – 20 15 – 20 No S deficiencya < 15 < 15 < 15
a Sulphur deficiency will likely be induced by N fertilization if N:S > 13
Revised March 2012
Revised foliar nutrient interpretative criteria Nutrient ratios cont’d
Nutrient ratio
Element Interpretation Pl Sx Fd
N:Mg Moderate to severe Mg deficiency > 30 > 30 > 30 Slight to moderate Mg deficiency 20 – 30 20 – 30 20 – 30 Possible slight Mg deficiency 15 – 20 15 – 20 15 – 20 No Mg deficiency < 15 < 15 < 15
Some basic interpretative rules
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Confirm that N deficiency exists before assessing other nutrients
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Confirm that N deficiency exists before assessing other nutrients
Assess S status in the following order of importance:
SO4 > N:S > SOnly assess N:S or S if SO4 status is marginal
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Confirm that N deficiency exists before assessing other nutrients
Assess S status in the following order of importance:SO4 > N:S > S
N:P, N:K, and N:Mg ratios are more important than absolute levels of P, K, or Mg
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Confirm that N deficiency exists before assessing other nutrients
Assess S status in the following order of importance:SO4 > N:S > S
N:P, N:K, and N:Mg ratios are more important than absolute levels of P, K, or Mg
Don’t be too concerned if possible deficiency is indicated for Cu, Zn, or Fe
Some basic interpretative rules
Confirm that standardized foliar sampling protocol was used
Assess foliar nutrient status within the context of site ecological characteristics
Confirm that N deficiency exists before assessing other nutrients
Assess S status in the following order of importance:SO4 > N:S > S
N:P, N:K, and N:Mg ratios are more important than absolute levels of P, K, or Mg
Don’t be too concerned if possible deficiency is indicated for Cu, Zn, or Fe
Ask for help if uncertain