Fluorescent Protein Fusion Expression System

8/8/2019 Fluorescent Protein Fusion Expression System

1/17

All l Bi t h I t d i C t Eff ti t R h

Fluorescent Protein

Fusion Expression System

For Research Use Only. Not for Diagnostic or Therapeutic Use. Purchase does not include or carry any right to resell or transferthis product either as a stand-alone product or as a component of another product. Any use of this product other than the permitteduse without the express written authorization of Allele Biotech is strictly prohibited

Box 1 | Product List

Fluorescent Protein Vectors

Vector Name Cat. Number

PagepmWasabi-C ABP-FP-WCNCS 2pmWasabi-N ABP-FP-WNNCS 3pmTFP1-C ABP-FP-TCNCS 4pmTFP1-N ABP-FP-TNNCS 5pLanRFP-C ABP-FP-RCNCS 6pLanRFP-N ABP-FP-RNNCS 7pLanYFP-C ABP-FP-YCNCS 8pLanYFP-N ABP-FP-YNNCS 9Vectors w/ Puromycin Resistance

pmWasabi-C-Puro ABP-FP-WCNCS 10pmWasabi-N-Puro ABP-FP-WNNCS 11pmTFP1-C-Puro ABP-FP-TCNCS 12pmTFP1-N-Puro ABP-FP-TNNCS 13pLanRFP-C-Puro ABP-FP-RCNCS 14pLanRFP-N-Puro ABP-FP-RNNCS 15pLanYFP-C-Puro ABP-FP-YCNCS 16pLanYFP-N-Puro ABP-FP-YNNCS 17

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2/17

CMV Promoter ........ 1-589

Multiple Cloning Sites ........ 591-646

mWasabi GFP ........ 653-1363

SV40 poly(A) Signal ........ 1529-1579

Ampicillin Resistance Gene ........ 2207-3067

pUC Origin ........ 3216-3858

Sequencing Primer:

CMV-F and mWasabi-R(ABP-FP-WASRPRM)

pmWasabi-CT (ABP-FP-WCNCS)

Multiple Cloning Sites

541 ACGGTGGGAG GTCTATATAA GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCTCTA

R * L *

601 GACTGGATCC TCGAATTCGG TACCTCAAGC TTGCGGCCGC CTCGAGGCCA CCATGGTGAG

T G S S N S V P Q A C G R L E A T M V S

661 CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC ATGAAGATCA AGCTGAAGAT

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1321 GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG TAA

NheI

BamHI EcoRI KpnI HindIII NotI XhoI

C


3/17


mWasabi GFP ........ 613-1320


SV40 poly(A) Signal ........ 1560-1610


pUC Origin ........ 3247-3889

Sequencing Primer:

mWasabi-F(ABP-FP-WASFPRM) and SV40pA-R

pmWasabi-NT (ABP-FP-WNNCS)


601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGACCACAA TGGGCGTAAT CAAGCCCGAC

661 ATGAAGATCA AGCTGAAGAT GGAGGGCAAC GTGAATGGCC ACGCCTTCGT GATCGAGGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 ACCGTTTACG AGATCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAGT V Y E I A V A R N S T D G M D E L Y K

1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC CGCTAA

S G L R S R V G S S N S V P S R Q A C G R

BglII XhoI BamH1 EcoRI KpnI HindIII NotI

N


4/17



mTFP1 TFP ........ 653-1363

SV40 poly(A) Signal ........ 1529-1579


pUC Origin ........ 3216-3858

Sequencing Primer:CMV-F and mTFP1-R(ABP-FP-TFPRPRM)


pmTFP1-CT (ABP-FP-TCNCS)




>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CATCGAGATC CTGAACCACG ACAAGGACTA CAACAAGGTG ACCGTTTACG AGAGCGCCGT


C


5/17



>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 ACCGTTTACG AGAGCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG

1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC

1381 CGCTAA


mTFP1 TFP ........ 613-1320


SV40 poly(A) Signal ........ 1560-1610


pUC Origin ........ 3247-3889

Sequencing Primer:mTFP1-F(ABP-FP-TFPFPRM) and SV40pA-R


pmTFP1-NT (ABP-FP-TNNCS)

N


6/17



661 CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC CACGATTTAC ACATCTCCGG

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CGTGTTCCGC AAGGTGGAAC TCAAGCACAC CAAGACTGAG CTCAACTTCA AGCAGTGGCA

1321 GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC AAGTAA



LanRFP ........ 653-1366

SV40 poly(A) Signal ........ 1532-1582


pUC Origin ........ 3219-3861

Sequencing Primer:CMV-F and LanRFP-R(ABP-FP-RFPRPRM)


pLanRFP-CT (ABP-FP-RCNCS)

C


7/17

601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC

661 CACGATTTAC ACATCTCCGG CTCAATCAAT GGACATGAGT TTGACTTGGA AGGCAGTGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CTCAACTTCA AGCAGTGGCA GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC

1321 AAGAGATCTC GAGTCGGATC CTCGAATTCG GTACCCTCTA GACAAGCTTG CGGCCGCTAA


LanRFP ........ 613-1323


SV40 poly(A) Signal ........ 1554-1604


pUC Origin ........ 3241-3883

Sequencing Primer:LanRFP-F(ABP-FP-RFPFPRM) and SV40pA-R


pLanRFP-NT (ABP-FP-RNNCS)

N


8/17


9/17

661 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA

721 CATGAGTTAC ACATCTTTGG CTCCTTCAAC GGTGTGGACT TTGACATGGT GGGTCGTGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1321AACTTCAAGG AGTGGCAAAA GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG

1381 AGATCTCGAG TCGGATCCTC GAATTCGGTA CCCTCTAGAC AAGCTTGCGG CCGCTAA


LanYFP ........ 613-1323


SV40 poly(A) Signal ........ 1554-1604


pUC Origin ........ 3241-3883

Sequencing Primer:LanYFP-F(ABP-FP-YFPFPRM) and SV40pA-R


pLanYFP-NT (ABP-FP-YNNCS)

N


10/17



mWasabi GFP ........ 653-1363

SV40 poly(A) Signal ........ 1529-1579

PGK Promoter ........ 1657-2159

Puromycin Resistance Gene ........ 2242-2841

SV40 poly(A) signal ........ 2965-3191


pUC Origin ........ 4850-5492

Sequencing Primer:

CMV-F and mWasabi-R(ABP-FP-WASRPRM)

pmWasabi-CT-PuroR (ABP-FP-WCNCSP)




>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CATCGAGATC CTGAACCACG ACAAGGACTA CAACAAGGTG ACCGTTTACG AGATCGCCGT



C


11/17


mWasabi GFP ........ 613-1320


SV40 poly(A) Signal ........ 1560-1610

PGK Promoter ........ 1688-2190Puromycin Resistance Gene ........ 2273-2872

SV40 poly(A) signal ........ 2996-3222


pUC Origin ........ 4881-5523

Sequencing Primer:

mWasabi-F(ABP-FP-WASFPRM)

pmWasabi-NT-PuroR (ABP-FP-WNNCSP)




>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>1261 ACCGTTTACG AGATCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG

T V Y E I A V A R N S T D G M D E L Y K

1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC CGCTAA

S G L R S R V G S S N S V P S R Q A C G R

BglII XhoI BamH1 EcoRI KpnI HindIII NotI

N


12/17



mTFP1 TFP ........ 653-1363

SV40 poly(A) Signal ........ 1394-1620

PGK Promoter ........ 1621-2123


SV40 poly(A) signal ........ 2929-3155


pUC Origin ........ 4575-5194

Sequencing Primer:

CMV-F and mTFP1-R(ABP-FP-TFPRPRM)

pmTFP1-CT-PuroR (ABP-FP-TCNCSP )


587 ATCCGCTAGCTCTAGACTGGATCCTCGAATTCGGTACCTCAAGCTTGCGGCCGCCTCGAGNheI XbaI BamHI EcoRI KpnI HindIII NotI XhoI

647 GCCACCATGGTGAGCAAGGGCGAGGAGACCACAATGGGCGTAATCAAGCCCGACATGAAG

707 ATCAAGCTGAAGATGGAGGGCAACGTGAATGGCCACGCCTTCGTGATCGAGGGCGAGGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1247 TTTGTGGACCACCGCATCGAGATCCTGAACCACGACAAGGACTACAACAAGGTGACCGTT

1307 TACGAGAGCGCCGTGGCCCGCAACTCCACCGACGGCATGGACGAGCTGTACAAGTAA


13/17


mTFP1 TFP ........ 613-1320


SV40 poly(A) Signal ........ 1560-1610

PGK Promoter ........ 1688-2190


SV40 poly(A) signal ........ 2996-3222


pUC Origin ........ 4881-5523

Sequencing Primer:

mTFP1-F(ABP-FP-TFPFPRM)


pmTFP1-NT-PuroR (ABP-FP-TNNCSP)



>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 ACCGTTTACG AGAGCGCCGT GGCCCGCAAC TCCACCGACG GCATGGACGA GCTGTACAAG

1321 TCCGGACTCA GATCTCGAGT CGGATCCTCG AATTCGGTAC CCTCTAGACA AGCTTGCGGC

1381 CGCTAA

N


14/17



LanRFP........ 653-1363

SV40 poly(A) Signal ........ 1532-1582

PGK Promoter ........ 1660-2162


SV40 poly(A) signal ........ 2968-3194


pUC Origin ........ 4853-5495

Sequencing Primer:

CMV-F and LanRFP-R(ABP-FP-RFPRPRM)

pLanRFP-CT-PuroR (ABP-FP-RCNCSP)




661 CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC CACGATTTAC ACATCTCCGG

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CGTGTTCCGC AAGGTGGAAC TCAAGCACAC CAAGACTGAG CTCAACTTCA AGCAGTGGCA

1321 GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC AAGTAA

C


15/17


LanRFP ........ 613-1323


SV40 poly(A) Signal ........ 1554-1604

PGK Promoter ........ 1682-2184


SV40 poly(A) signal ........ 2990-3216


pUC Origin ........ 4875-5517

Sequencing Primer:

LanRFP-F(ABP-FP-RFPFPRM)


pLanRFP-NT-PuroR (ABP-FP-RNNCSP)

601 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCCCTCT CCCAGCAACC

661 CACGATTTAC ACATCTCCGG CTCAATCAAT GGACATGAGT TTGACTTGGA AGGCAGTGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 CTCAACTTCA AGCAGTGGCA GAAGGCATTC CAGGACATCG CCGGCATGGA CGAGCTGTAC

1321 AAGAGATCTC GAGTCGGATC CTCGAATTCG GTACCCTCTA GACAAGCTTG CGGCCGCTAA

N


16/17



LanYFP ........ 653-1363

SV40 poly(A) Signal ........ 1529-1579

PGK Promoter ........ 1657-2159


SV40 poly(A) signal ........ 2965-3191


pUC Origin ........ 4850-5492

Sequencing Primer:

CMV-F and LanYFP-R(ABP-FP-YFPRPRM)

pLanYFP-CT-PuroR (ABP-FP-YCNCSP)




661 CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA CATGAGTTAC ACATCTTTGG

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1261 GTTCCGTAAG ACGGAGCTCA AGCACTCCAA GACCGAGCTC AACTTCAAGG AGTGGCAAAA

1321 GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG TAA

C


17/17


LanYFP ........ 613-1323


SV40 poly(A) Signal ........ 1554-1604

PGK Promoter ........ 1682-2184


SV40 poly(A) signal ........ 2990-3216


pUC Origin ........ 4875-5517

Sequencing Primer:

LanYFP-F(ABP-FP-YFPFPRM)


pLanYFP-NT-PuroR (ABP-FP-YNNCSP)

661 CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGGATAACA TGGCCTCTCT CCCAGCGACA

721 CATGAGTTAC ACATCTTTGG CTCCTTCAAC GGTGTGGACT TTGACATGGT GGGTCGTGGC

>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>

1321AACTTCAAGG AGTGGCAAAA GGCCTTTACC GATGTGATGG GCATGGACGA GCTGTACAAG

1381 AGATCTCGAG TCGGATCCTC GAATTCGGTA CCCTCTAGAC AAGCTTGCGG CCGCTAA

N

Fluorescent Protein Fusion Expression System

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