Fluconazole - eucast
Transcript of Fluconazole - eucast
EUCAST Fluconazole rationale document version 1.0 Page 1 of 13
Fluconazole Rationale for the EUCAST clinical breakpoints, version 1.0 5 April 2007 Introduction Fluconazole is an azole antifungal agent active against Candida species and Cryptococcus species. It is considered appropriate therapy for candidaemia among neutropenic and non-neutropenic patients, chronic disseminated candidosis, disseminated cutaneous neonatal candidosis, urinary tract infections, lower respiratory tract infections, osteomyelitis, arthritis, infections of gallbladder, pancreas and peritoneum, endocarditis, pericarditis, suppurative phlebitis, myocarditis, meningitis and endophthalmitis due to Candida species, non-genital mucocutaneous candidosis and genital candidosis In addition, fluconazole is considered appropriate prophylaxis for neutropenic patients particularly if colonised with Candida tropicalis, allogeneic haematological stem cell transplant (HSCT) recipients and recipients of liver transplants. The activity in vitro of fluconazole against species of Candida is not uniform. The species more frequent associated with human infections include C. albicans, C. parapsilosis and C. tropicalis, which usually exhibit low fluconazole MIC values, but a significant number involve C. glabrata, which usually exhibits high MICs. In addition, fluconazole is not indicated for treating any infection caused by C. krusei, which is considered inherently resistant to fluconazole. Therefore, every attempt should be made to identify Candida to species level. The EUCAST-AFST (European Committee on Antimicrobial susceptibility Testing – subcommittee on Antifungal Susceptibility Testing) has determined breakpoints for fluconazole for Candida species.
EUCAST
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1. Dosage
Aus
tria
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Most common
dose (mg/day)
400 400 400 400 400 400 400 400 400 400 400 400 400
Maximum dose
(mg/day) 800 800 800 800 800 800 800 800 800 800 800 800 800
Available formulations
Capsules, iv
Capsules, iv
Capsules, iv
Capsules, iv
Capsules, iv, oral susp-ension
Capsules, iv
Capsules, iv
Capsules, iv, oral susp-ension
Capsules, iv
Capsules, iv
Capsules, iv
Capsules, iv
Capsules, iv
EUCAST
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2. MIC distributions and epidemiological cut-off (ECOFF) values
Species 0.016 0.032 0.064 0.125 0.25 0.5 1 2 4 8 16 32 64 128 256 512 ECOFF≤ (mg/L)*
Candida albicans 14 57 275 4039 7062 2857 1112 443 141 157 109 92 249 92 35 1 1
Candida glabrata 2 7 5 14 11 48 110 323 917 1271 1414 556 269 166 75 26 32
Candida krusei 0 0 0 0 0 0 0 0 1 9 50 320 238 61 11 0 128
Candida parapsilosis 7 50 10 78 447 1134 791 328 89 62 44 14 5 2 2 1 2
Candida tropicalis 0 13 22 151 522 928 706 418 112 40 37 14 16 39 4 3 2
*ECOFF, epidemiological cut-off value (mg/L) as defined by EUCAST
EUCAST
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3. Breakpoints before harmonisation (mg/L) S< R> DIN NWGA CLSI1 General Breakpoints 4 / 16 4 / 32 8 / 16-32 / 64
1CLSI states that isolates for which the MIC of fluconazole is 16-32 mg/L are considered susceptible dependent upon dose (S-DD), on the basis of data indicating a satisfactory clinical response when at least 100 mg/day of fluconazole is given. The novel category "susceptibility dose/delivery dependent" (S-DD) indicates that maximization of dosage and bioavailability are critical to successful therapy. The CLSI breakpoints do not apply to C. krusei, which is considered inherently resistant to fluconazole.
EUCAST
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4. Pharmacokinetics Dosage (mg) 400/day iv 800/day iv Cmax (mg/L) 18.9-30.6 34 Cmin (mg/L) 21-23 (IV steady state) Total body clearance (L/h) T ½ (h) 31-37.2 31-37.2 AUC24h (mg.h/L) 350 813.27 Fraction unbound (%) 88-89 88-89 Volume of distribution (L/kg) 0.7-0.8 0.7-0.8 Comments
References • Brammer et al., Rev.Infect.Dis. 1990; Suppl 3: S318-S326 • Grant & Clissold, Drugs 1990; 39:877-916 • Goa & Barradell, Drugs1995; 50: 658-690
EUCAST
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5. Pharmacodynamics Candida spp fAUC/MIC for fungistasis 25 – 50 fAUC/MIC for 2 log drop in 24h 500 – 1000 f/AUC/MIC from clinical data 100
Comments
• The trailing effect and a fAUC/MIC of 500-1000 needed to obtain a 2 log reduction in growth in 24h are derived from in vitro and in vivo data showing the fungistatic nature of fluconazole. Therefore, for invasive candidosis it is recommended that an fAUC/MIC for fungistasis of at least 100 is attained. This target is supported by clinical data showed in table 2 of point 7, in this document.
References
• Grant & Clissold. Drugs 1990; 39: 877-916 • Goa & Barradell, Drugs 1995; 50: 658-690 • Andes & van Ogtrop, AAC 1999; 43: 2116-2120 • Andes, AAC 2003; 47:1179-1186
EUCAST
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6. Monte Carlo simulations and Pk/Pd breakpoints Probabilities of Target Attainment for 400 mg/day iv are shown in Figure 1.
Figure 1. Probabilities of Target Attainment for fluconazole for 400 mg/day iv in steady state. The horizontal lines indicate a Pk/Pd target of fAUC/MIC of 100 and 50. The following pharmacokinetic parameters were used to obtain the PTA: Volume of distribution (Vd): 45L, CV 12% Half-life: 32 h, CV 15% Fraction unbound (Fu): 88% Results of simulations for the 400 mg oral dose do not markedly influence conclusions.
1 2 4 8 16 32
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100095% percentile
mean99% percentile
MIC mg/L
fAU
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1 2 4 8 16 32
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1 2 4 8 16 32
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mean99% percentile
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EUCAST
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7. Clinical data There is an almost 1:1 linear relationship between the AUC and the dose of fluconazole. There is also a direct though imperfect relationship between the AUC or dose and a successful clinical response of oral candidosis and to a lesser extent candidaemia to treatment. Similarly cure is less likely for infections caused by strains with a higher MIC. In the tables below the dose/MIC is shown. The data were obtained after correlating the clinical outcome with MICs obtained by means of EUCAST methodology. More than 90% of patients respond to treatment irrespective of the dose when the MIC of the yeast was ≤ 2 mg/L (Table 1). When considering only doses >100 mg/day, all patients with isolates with MICs of ≤ 4 mg/L responded to treatment. When the dose/MIC is ≥100, as shown in Table 2, more than 90% of patients respond to treatment. (Rodriguez-Tudela JL et al, Antimicrob Agents Chmother, in press) Table 1. Correlation of fluconazole MIC with treatment outcome for different fluconazole doses
% Clinical success (number cured/total) for indicated fluconazole dose
100 mg/day >100 mg/day All doses
MIC (mg/L) Candidaemia OPC Candidaemia OPC Candidaemia OPC
All cases % Clinical success
(n cured/n total)
< 0.5 75 (3/4) 100 (21/21) 92 (95/103) 100 (5/5) 91 (98/107) 100 (26/26) 93 (124/133)
1 - 100 (4/4) 100 (6/6) - 100 (6/6) 100 (4/4) 100 (10/10)
2 - 100 (1/1) 100 (1/1) - 100 (1/1) 100 (1/1) 100 (2/2)
4 - 20 (1/5) 100 (3/3) 100 (4/4) 100 (3/3) 69 (5/9) 66 (8/12)
8 - 0 (0/15) 40 (2/5) 41 (7/17) 40 (2/5) 26 (7/32) 24 (9/37)
> 16 -- 0 (0/19) 75 (3/4) 0 (0/41) 75 (3/4) 2 (0/60) 4 (3/64)
EUCAST
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Table 2. Correlation of dose/MIC (and their conversions to AUC/MIC1 and fAUC/MIC2 ratios) and clinical outcome for patients with candidemia or oropharyngeal candidiasis (OPC)
% Clinical success (n cured/n total) Dose/MIC AUC/MIC fAUC/MIC
Range of doses administered
Range of MICs (mg/L) of isolates Candidaemia OPC All
400-4,800 359-4,678 316- 4,117 100-800 0.12-1.0 92 (102/111) 100 (5/5) 92 (107/116)
150-200 146-189 129-166 100-600 0.5-4.0 100 (3/3) 100 (21/21) 100 (24/24)
100 90 79 100-800 1.0-8.0 100 (4/4) 100 (5/5) 100 (9/9)
50 45 40 100-400 2.0-8.0 33 (1/3) 57 (4/7) 50 (5/10)
25 22 20 100-200 4.0-16.0 50 (1/2) 42 (8/19) 42 (9/21)
12.5 11 10 100-400 8.0-32.0 100 (1/1) 0 (0/34) 3 (1/35)
6.25 6 5 200 32.0 100 (1/1) 0 (0/22) 4 (1/23)
3.13 3 2 100-200 32.0-64.0 0 (0/1) 0 (0/19) 0 (0/20)
1The values of AUC/MIC were obtained by applying the following equation: AUC = 0.99 x Dose – 9.2 2fAUC/MIC was calculated taking in consideration that the fraction of unbound fluconazole is 88% EUCAST
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8. Clinical breakpoints
Non-species-related breakpoints
These have been determined mainly on the basis of Pk/Pd data and are independent of MIC distributions of specific species. EUCAST does not advocate terms such as SDD (susceptible dose-dependent) preferring to use “I” (intermediate) to denote strains that are considered neither susceptible nor resistant. The column of non-species related breakpoints is for use only for species not included in the table. They should not be used for species where susceptibility testing is not recommended (marked with -- or IE in the EUCAST breakpoint tables).
Non-species-related breakpoints are S≤2 mg/L, R>4 mg/L.
Species-related breakpoints
The in vitro activity of fluconazole against species of Candida is not uniform. The species most frequently associated with human infections are C. albicans, C. parapsilosis and C. tropicalis. These exhibit MIC values of ≤2 mg/L when without resistance mechanisms to fluconazole. C. albicans S≤2, R>4 mg/L C. tropicalis S≤2, R>4 mg/L C. parapsilosis S≤2, R>4 mg/L
Species without breakpoints
A significant number of infections involve C. glabrata, which exhibits fluconazole MICs of 2-32 mg/L when without resistance mechanisms, and C. krusei, which is considered inherently resistant with even higher MIC values, also when without resistance mechanisms. Any reasonable breakpoint would divide wild type C. glabrata, obviating reproducible susceptibility testing. For these reasons, EUCAST-AFST has refrained from giving C. krusei and C. glabrata breakpoints for fluconazole and advise that other drugs be chosen for treating infections caused by these species. As there are few drugs suitable for the treatment of urinary tract infections caused by Candida spp. fluconazole may be a suitable choice for C. glabrata infections regardless of the MIC, as fluconazole is concentrated in the urine. However, this emphasises the need for correct species identification.
Clinical qualifications
The EUCAST-AFST considers fluconazole appropriate therapy for: • Candidaemia among neutropenic and non-neutropenic patients • Chronic disseminated candidosis • Disseminated cutaneous neonatal candidosis • Urinary tract infections, lower respiratory tract infections, osteomyelitis, arthritis, infections of gallbladder,
pancreas and peritoneum, endocarditis, pericarditis, suppurative phlebitis, myocarditis, meningitis and endophthalmitis due to Candida species
• non-genital mucocutaneous candidosis • Genital candidosis
EUCAST
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The EUCAST-AFST considers fluconazole appropriate prophylaxis for:
• Neutropenic patients particularly if colonised with Candida tropicalis • Allogeneic HSCT recipients • High-risk recipients of liver transplants
Dosage The EUCAST breakpoints apply to oral and intravenous administration of fluconazole of 400–800 mg per day. Additional comment
EUCAST
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9. Fluconazole - EUCAST clinical MIC breakpoints These can be found at http://www.eucast.org.
EUCAST