Located at the Houston Methodist Research Institute in Houston, TX, the Flow Cytometry Core is staffed by experienced research professionals and laboratory technicians who provide training, instrumentation, technical expertise and software for flow cytometric analysis and cell sorting.

We offer comprehensive analyzer training, assistance in the design of experimental protocols, and ongoing consultation and support. We look forward to serving you with customized projects that will enhance your activities in a reliable and timely manner.

Flow Cytometry Core

Director: David L. Haviland, Ph.D., C. Cy.Email: dlhaviland@houstonmethodist.org713.441.9233

houstonmethodist.org/flowcytometry

FLOW CYTOMETRY CORE

HOUSTON METHODISTRESEARCH INSTITUTE

Houston Methodist Research Institute

6670 Bertner Avenue

Houston, TX 77030

houstonmethodist.org/research

HMRI Communications & External Relations | RICORE-004 | RH | DH | 500 | 02.2013

Flow Cytometry Core

The Houston Methodist Research Institute Flow Cytometry Core provides training, instrumentation, technical expertise and software for flow cytometric analysis and cell sorting.

FACILITY

The Flow Cytometry Core uses fluorescent probes, usually labeled antibodies, to identify cells. Information about the reative size and granularity of a cell is recorded and the result is a visual presentation describing an individual or group of cellular events. If needed, the cells or particles can be separated by sorting and further analyzed.

FLOW CYTOMETRY EQUIPMENT

These instruments allow scientist to make very concise measurements at the cellular level, evaluate a large number of samples in a short time frame, and gather information on very rare populations of cells.

• BD FACS LSRII - A five laser instrument with 355, 405, 488, 561, and 630 nm excitation sources, capable of 14 color analysis

• BD FACS Fortessa - A four laser instrument with 405, 488, 561, and 630 nm excitation sources, capable of 13 color analysis, and equipped with a Forward Scatter PMT in addition to the FSC photodiode

• NIR BD FACS AriaII - A six laser instrument with 355, 405, 488, 550, 635, and 785 nm excitation sources, capable of 18 color analysis. This instrument is equipped with an Forward Scatter PMT in addition to the FSC photodiode. This instrument is capable of high speed sorting, sample depending. Temperature controlled sample and collection chambers with four-way and single cell deposition are available.

• SORP BD FACS AriaII – A four laser instrument with 405, 488, 550, and 635 nm excitation sources capable of 13 color detection. This instrument is also capable of high speed sorting, sample depending. Temperature controlled sample and collection chambers with four-way and single cell deposition are available.

• A Millipore Guava 8-HT – A two laser instrument with 488 and 635 nm excitation sources capable of six color detection. The 8-HT provides rapid 96-well handling capability for screening assays.

OurSpecializedServices

Flow Cytometry core staff will advise on:

• Experimental design

• Sample preparation

• Controls

• Excitation and detection

• Data Interpretation

• Manuscript preparationLSRI

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