8/8/2019 Fishfeed Protien Analysis

1/3

8/8/2019 Fishfeed Protien Analysis

2/3

Protein, Carbohydrate, Fat and Moisture in Fish Feed 2

Initial ingredients

Figure 2 Application of low field NMR in quality control during fish feed production

Figure 3 User interface of an application for the determination of protein, carbohydrate, fat and

moisture in fish feed implemented in the minispec mq10 low field NMR analyser (Bruker Optik, GmbH,

Germany)

Protein and Carboh drate

Fat+Water Fat

8/8/2019 Fishfeed Protien Analysis

3/3

Protein, Carbohydrate, Fat and Moisture in Fish Feed 3

ANALYSIS OF FISH FEEDA new low field NMR method for

determination of protein, carbohydrate, fat and

moisture content in fish feed has been

developed and implemented in the Bruker

minispec mq10 low field NMR analyser. Allthe mentioned parameters are determined

simultaneously in one measurement. The new

method is based on a combination of the well-

known solid echo, free induction decay

and spin echo NMR sequences.

The NMR instrument operates at proton

frequency of 10 MHz and has a 40mm bore

opening. A typical fish feed sample consists of

about 30 or more pellets depending on their

size.

Prior to the measurements, the instrumentresponse has to be calibrated against a set of

reference samples with known protein,

carbohydrate, fat and moisture contents. For

protein and carbohydrate calibrations, the

reference sample set must contain the raw

ingredients as well. The program user interface

and a typical NMR response is shown in

Figure 3.

NMR response is not sensitive to minor

changes in the microscopic properties of the

raw materials used in the production being

directly proportional to the total quantity of themeasured compound. Origin or type of fish oil

and other raw ingredients do not have any

significant impact on the observed NMR

signals. Therefore, it is not necessary to

recalibrate the instrument for new raw material

batches supplied from different sources.

Reliable determination of the protein and

carbohydrate content with this technique

suggests that the fish feed recipe is available.

The method has been successfully tested in the

research fish feed factory (EWOS Innovation

Ltd., Dirdal, Norway) and has proven to be

quick, precise, and robust.

SUMMARYThe new low field NMR technique allows a

quick and non-destructive determination of the

main constituents in fish feed. No toxic

solvents are needed and the analysis can be

performed by ground-floor personnel. The low

field NMR equipment requires nomaintenance. These obvious advantages make

the low field NMR technique highly

competitive with both traditional chemical

analyses and Near Infrared Spectroscopy.

The new technique has a potential to be

implemented for an automated on-line quality

control of fish feed production.

The present low field NMR technique can be

further modified and customised to determine

similar quality-related parameters in other

types of fodder as well as in a wide range ofdry food products.

For more information contact:

SINTEF Fisheries and Aquaculture

N-7465 Trondheim, NORWAY

http://www.sintef.no

Emil Veliyulin

E-mail: emil.veliyulin@sintef.no

Phone: (+47) 73 55 13 56

Fax: (+47) 73 86 77 08

Bruker Optik GmbH

Silberstreifen

D-76287, Rheinstetten, GERMANY

http://www.minispec.com

E-mail: minispec@bruker.de

Phone: (+49) (721) 5161 141

Fax: (+49) (721) 5161 237