Finding a morphogen gradient using forward...
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Finding a morphogen gradient using
forward genetics:
Dorsal-ventral patterning by BMPs in
Drosophila embryos
Laurel RafteryCBRCMGH/Harvard Med. School11/15/2006
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Drosophila melanogasterwww.istoica.com/.../ 20060618174337_genetics.jpg
Artist s conception
Fruit flies…
www.hubbo.com/images/ Fly-and-banana.jpg
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Drosophila life cycle:
two distinct developmental phases
embryonic
development
metamorphosis
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Drosophila development:
two different bodies for two different purposes
feeding
Dispersal and
reproduction
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
•Computational predictions of molecularmechanisms
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
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Why I chose to study Drosophila development:
the most powerful systemto dissect the logic ofdevelopment
Nobel prize
Nusslein-Volhard and Wieschaus
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From: Scott F. Gilbert, 2000. Developmental Biology 6th Edition
Ed Lewis:Homeotic mutations Homeobox genes
“…and when that happened
a revolution occurred in the field of biology”
-Scott Gilbert
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From: Swalla, 2006, Heredity 97: 235-243
Homeobox genes establish body plans for all multicellular organisms
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Why people choose Drosophila today:
•Multicellular organisms use the same tools•e.g. the gang of five for organogenesis:
•TGF ligands: BMPs*•Receptor tyrosine kinase ligands: EGFs and FGFs•Hedgehogs*•Wnts*•Notch ligands*
•It is easier to understand a simple system•e.g. 7 TGF ligands in flies
57 in mice 34 in zebrafish
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Practical reasons to study flies:•They have a 10 day generation time•They are inexpensive to rear•Genetic tools are sophisticated and easy to use
•Because flies are fast and inexpensive you can•Develop a developmental hypothesis•test it•Define a molecular mechanism•Test it
all in one graduate student s thesis project
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Practical reasons to study flies:•They have a 10 day generation time•They are inexpensive to rear•Genetic tools are sophisticated and easy to use
•Regulators don t care about flies•People share reagents, even before publication
•Because flies are fast and inexpensive you can•Develop a developmental hypothesis•test it•Define a molecular mechanism•Test it
all in one graduate student s thesis project
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
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•Different cell fates can bedetermined by differentconcentrations of amorphogen, a diffusiblemolecule present at variableconcentrations(A. Turing, 1952)
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A cell decides its fate basedon its position within thetissue. The pattern of cellfates is established through acoordinate system ofpositional information.(L. Wolpert, 1969)
2002 Curr. Biol.
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Amount
DISTANCE
Gradient
Thresholds for responses
Source Sink
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
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The Drosophila body plan is prepatterned
in the egg
By RNA and protein from the mother
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AnteriorPosterior
Dorsal
Ventral
mouth
hooks
posterior
spiracles
denticle
bands
The Drosophila body plan is visible in the
exoskeleton (cuticle)
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Anterior System head & thorax
Posterior System thorax & abdomen
Terminal System head and tail
D-V System
A set of maternal genes establishes each
aspect of the body plan
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reviewed by St Johnston & N sslein Volhard Cell
Maternal genes were identified by embryo phenotypes
Anteriorposteriortermini anddorsal-ventral
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Maternal genes:NF- B gradient subdivides mesoderm/ectoderm
Zygotic genes:BMP gradient subdivides ectodermal territories
Dorsal-ventral axis is
patterned by sequential
morphogen gradients
St Johnston & N sslein Volhard Cell
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Why a gradient of Dpp in embryos?
•Dpp was known to be a BMP, andhypothesized as a morphogen for appendagedevelopment.
•Uniform RNA levels, protein undetectableAn inference from the genetic analysis ofectoderm patterning
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Why a gradient of Dpp in embryos?
•Different Dpp mutations cause loss of moreor fewer dorsal ectoderm structures•WWharton et al Development
•Dpp was known to be a BMP, andhypothesized as a morphogen for appendagedevelopment.
•Uniform RNA levels, protein undetectableAn inference from the genetic analysis ofectoderm patterning
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-/-
Different mutations indpp eliminate differentamounts of dorsal fates
Irish & Gelbart Genes Dev
Wharton et al Development
+/+
+/-
Dpp is necessary to makedorsal ectoderm andamnioserosa
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The less DPP, the fewer dorsal structures
Mesoderm
AmnioserosaAnterior Posterior
Dorsal Ectoderm
Ventral Neurogenic Ectoderm
Lateral View
Wharton et al Development
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Why a gradient of Dpp in embryos?
•Different Dpp mutations cause loss of moreor fewer dorsal ectoderm structures•WWharton et al Development
•Injections of Dpp RNA induced dorsal fates•FFerguson and Anderson Cell
•Uniform RNA levels, protein undetectable
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Dpp RNA is sufficient to induce dorsal fates
zen is expressed inamnioserosa primordium
DppRNA
dorsal dorsal
ventral ventral
zen expression is induced inventral cells by Dpp RNA
Ferguson & Anderson Cell
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
![Page 30: Finding a morphogen gradient using forward geneticsweb.mit.edu/7.72/restricted/pdfs/772Class11-15-06.pdf · •Why Drosophila genetics? •What is a morphogen gradient? •Inference](https://reader033.fdocuments.in/reader033/viewer/2022052520/6081bec0010d303d2845e015/html5/thumbnails/30.jpg)
Mutational screens identified classes of
genes with shared D-V patterning defects
Arora et al
Development
* *
* * * * *
tld and scware almost assevere as dpp
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tld
sog
Dpp zen
Ferguson & Anderson Development
Dpp activity is modulated by tld and sog
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Mouse 57Fly 7Worm 4
BMPs(bone morphogenetic proteins)
Dpp and Screw are
TGF proteins organizedaccording to sequence similarity:A phylogenetic tree
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Experimental tests saythat the most similarBMPs are from differentspecies:
Fly DPP can induce boneformation in rats.
Mouse BMP2 can replacefly DPP in dorsal-ventralpatterning.
DPP, BMP2, BMP4are orthologs
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BMPs regulate nested patterns of gene expression
Amnio-serosa
Ventral Neurogenic Ectoderm
MS
Dorso-medialEctoderm
Dorso-lateralEctoderm
Race
hindsight
ushtup
pannier
dpp
reviewed by
Raftery & Sutherland Trends Genet
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Model: Sog removes BMPs at low end of gradient
Jazwinska et al
Development
Sog binds Dpp
reviewed by Bier Nature
Screw is everywhere
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[BMP]
Model: a BMP activity gradient at blastoderminduces dorsal ectoderm fates
2 BMPs:Dpp+Screw
Amnioserosa
Dpp
DorsalEctoderm
Sog
VentralNeurectoderm
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How do cells respond to the BMP gradient?Mad and Medea identified in screens for genes that
interact with Dpp
transcription
P P
serinephosphorylation
receptoractivation
tissue-specific transcription factor
ligand binding
?Mad?Medea?
?
?
Raftery et al Genetics
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Smad proteins transduce TGF family
signals to the nucleus
transcription
oligomerization
P P
serinephosphorylation
receptoractivation
PP
PP
Mad=R-Smad
Medea=co-Smad
tissue-specific transcription factor
Reviewed by Raftery & Sutherland Dev Biol
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
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How can we visualize a BMP activity gradient?
•Dpp RNA expression is low•Dpp protein hasn t been visualized•Graded distribution of diffusible antagonist, Sog
Srinavasan et al Dev Cell
Look at distribution of activated signal transducers
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P
P
P
P
type I type II
ligand
R-Smadco-Smad
receptor subunits
P
PP
ligands Dpp Screw
type Ireceptor
Tkv Sax
MadR-Smad
co-Smad Medea
direct patterning ofdorsal ectodermal fates
BMP4 BMP7
Medea nuclear localization and phospho-Madpredicted to indicate level of BMP activity in vivo
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A dorsal midline stripe of strong nuclear Medea
End blastodermSide view
2.75hr
GastrulationDorsal view
3.25hr
Sutherland et al Development
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BUT…?
Stripe of intense response is only wide enough toaccount for amnioserosa induction…
Can we detect a broader signal?
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A low level signal in some blastoderm embryos
D
V
32 cells wide 3-9 cells wide
Medea
DNA
uniform cellular blastoderm cephalic furrow initiated
Sutherland et al
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co-Smad response domain narrows as peakforms at the dorsal midline
strongnuc. Med
Uniform Medno nuclear
signal
32 cells 3-9 cells
transient, oroccasional
every embryo at theonset of gastrulation
Ross et al, 2001 and Rushlow et al, 2001 have similarobservations for PMad
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Both co-Smad and phospho-Madresponses elevate during gastrulation
Sutherland et al
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During gastrulation, peak co-Smad response isfurther elevated and more cells are involved
declining nuc. Med+ PMad
uniform Med
no nuclear signal
peak nuc. Med+P-Mad
about 18 cells
7-9
5-7 cells
3-4
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uniform Medea+ low P-Mad
wanng nuc. Med +P-Mad
No detectable signal
peak nuc. Med +P-Mad
C F I
1st gradient transitionA D G
B E H
Phase I Phase II Phase III2nd gradient
D
V
Raftery and Sutherland
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
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How is the BMP gradient shaped?
Wide band weak signal
Narrow stripe strong signal
Mid-blastoderm
Gastrulation
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type I type II
R-Smadco-Smad
receptor subunits
Sog
Sog is an extracellular BMP binding proteinthat can block signaling
Jazwinska et al, 1999,Development 126, 3323
but, Sog is also requiredto get a normal number ofamnioserosa cells
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[BMP]
How does Sog affect the nuclear co-Smadsignal?
2 BMPs:Dpp+Screw
Amnioserosa
Dpp
DorsalEctoderm
Sog
VentralNeurectoderm
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Sutherland et al
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Sog shapes the BMP activity gradient over time
WT
sog-
quenches promotes
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Modulation of BMP levels: 1992 to 2002tld
sog
Dpp zen
Tld=Metalloprotease
Sog=BMP binding protein
Zen=transcription factor,Partner to Smads
Reviewed by Meinhardt & Roth Dev Biol
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But how does this work?
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O'Connor, M. B. et al. Development 2006;133:183-193
A BMP transport model:
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•Why Drosophila genetics?
•What is a morphogen gradient?
•Inference of a morphogen gradient from genetics•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers•Shaping the gradient
•Computational predictions of molecularmechanisms
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Free ligand concentration :
Ligand bound to receptor:
Sog bound to Tld:
Sog concentration over time:
Tld concentration over time:
Ligand bound to Sog and Tld:
Changing BMP gradient can be describedmathematically
Mizutani et al Dev Cell :
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Mizutani et al Dev Cell :
Computational modeling of the changingpattern of responses…
Can be used to predict important parameters
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•Degradation of ligand-receptor complexesMizutani et al Dev Cell :
•Positive feedback to stabilize narrow, high levelgradientO Connor et al Development :
•Contribution of Screw ligand: heterodimers versus twohomodimersShimmi et al Cell :
Computational modeling to test theimportance of molecular mechanisms…
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We still have questions…
•Pathway genetics
•Prediction of a morphogen gradient
•Identification of signaling pathway from genetics
•Visualization of a gradient from signal transducers
•Computational predictions of molecularmechanisms
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How is a threshold response organized
to make a tissue boundary?
amnioserosa/dorsal ectoderm boundary