FINDING 16S

BY JADINE DALEY

New Domain

16S rRNA

rRNA Found in all Bacteria Functional role is same in all ribosomes Structure changes slowly with time Contains variable and conserved regions

Used as standard for classifying and identifying microbes

Technique pioneered by Carl Woese and George E. Fox in 1977

16S in PCR

In our PCR experiment we...

Mixed our purified DNA with Primers and Master Mix to target 16S rRNA gene

Placed in thermocycler to amplify

Loaded amplified DNA into gel

Ran Gel Electrophoresis

Visualized gel

Celia Moore, PhDProfessor of Psychology, College

of Liberal Arts

Areas of Expertise

Developmental Psychobiology

Behavioral Neuroscience

Hormones and Behavior

Sexual Differentiation