Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed...

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The Evaluation and Expansion of the Solar Disinfection Method for Reclaimed Residential Greywater

Transcript of Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed...

Page 1: Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed Residential Greywater

The Evaluation and Expansion of the Solar

Disinfection Method for Reclaimed Residential

Greywater

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Contents

EXECUTIVE SUMMARY ........................................................................................................................ 3

A. Summary of Phase I Results .................................................................................................................... 9

Background and Problem Definition ........................................................................................................ 9

Purpose, Objective and Scope ................................................................................................................. 10

Data, Findings, Outputs/Outcomes ......................................................................................................... 10

Biotechnology ........................................................................................................................................ 12

Design Optimization and Fabrication ................................................................................................. 16

Discussion, Conclusions, Recommendations .......................................................................................... 18

Assurance that research misconduct has not occurred during the reporting period ............................ 19

B. Proposal for Phase II ............................................................................................................................... 20

P3 Phase II Project Description ............................................................................................................... 20

Quality Assurance Statement ................................................................................................................. 22

Project Schedule ..................................................................................................................................... 23

EPA Human Subjects Research Statement ............................................................................................. 24

C. References .............................................................................................................................................. 25

Attachments ............................................................................................................................................ 28

Budget ......................................................................................................................................................... 30

Budget Justification .................................................................................................................................... 32

Relevance and Past Performance ................................................................................................................ 34

Relevance to EPA ................................................................................................................................... 34

Past Performance of the Principal Investigator: ...................................................................................... 34

Resumes ...................................................................................................................................................... 35

Dr. Patricia Phelps .................................................................................................................................. 35

Kristine Lilly ........................................................................................................................................... 35

Student Bios ........................................................................................................................................... 36

Current and Pending Support ...................................................................................................................... 42

Confidentiality ............................................................................................................................................ 42

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EXECUTIVE SUMMARY

NCER Assistance Agreement Project Report Executive Summary

Date of Project Report: 03/12/2015

EPA Agreement Number: SU835926

Project Title: Evaluation and Expansion of Solar Disinfection Method for Residential

Greywater

Faculty Advisor(s), Departments and Institutions: Dr. Patricia Phelps, Biology, Dr. George

Staff, Department Chair of Earth Sciences, Austin Community College

Student Team Members, Departments and Institutions: Kelli Boydston, Emma Drueke,

Christina Edgar, Mariah Farrar, Stephanie Gage, Andrea Gonzalez, Samer Hasan, Michael

Hixson, Isabelle Jaimes, Ben Jeffries (Lead), Caitlyn Lankford, Katy Leatham, Michelle

McGill, Kristine Lilly (Author - Project Manager), Paul Newcomb, Miranda Peterson, Dylan

Reynolds, Elizabeth Savercool, Matthew Schulze, Marcus Searle, G.P. Selvaggio, (Lead),

Chynna Spangle, Jered Staton, Kevin Strickland, (Lead), Thomas Thompson, Josh Walden

Project Period: 8/31/2014 – 8/30/2015

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The Evaluation and Expansion of the Solar Disinfection

Method for Residential Greywater

Description and Objective of Research: The solar disinfection

of greywater (SODIS), is a method that has been developed and

proven as a means to produce potable water in a low-cost,

effective, and nontoxic manner. Currently over 2 million people

in 28 developing countries use the SODIS method for daily

drinking water (Centers for Disease Control. 2012).

Issues of water scarcity affect every continent on the planet.

More than 1.6 billion people live in areas suffering from water

scarcity, and more than 500 million people are approaching this

situation. This problem encompasses over one fourth of the

…Issues of

Water

Scarcity…

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world’s population (UNDESA,

U. N. 2011). In the United

States, the challenges of water

scarcity issues are being

exacerbated by intense drought

across the entire Southwest,

including California, Texas and

Oklahoma (USDM, U. S. 2015).

Water shortage within the U.S. is

not just an environmental

concern when our current daily

demand for water threatens the

availability of this precious

resource in the future. A crisis

may soon emerge into other

areas of the U.S. when local

surface and groundwater sources

can no longer support our

increasing demand for water

(GreenFacts. 2008). It is

estimated that there will be a 50% shortfall of water supply for many counties in central Texas

within the next two decades based on current water usage and demographics (USDM, U. S.

2015).

In Phase I of this research project, the Austin Community

College S-STEM research team has proposed that solar

disinfection (SODIS) can be used in the United States as a way

to recycle greywater in a residential setting. “Greywater is all

reusable wastewater from residential […] bathroom sinks, bath

tub shower drains, and clothes washing equipment drains”

(EPA Region IX, 1998). Water can be recycled just like any

other recycling medium such as aluminum, paper and plastic.

Its process is aptly named greywater recycling. Recycled

greywater can be used in many applications around the home

and can provide safe irrigation water for lawns and gardens

while also reducing the amount of water each home uses.

While the SODIS method is well established to produce

drinking water without chemicals or energy by using recycled

plastic bottles, the process is limited by the amount of water that

Figure 1: Drought conditions are impacting the majority of

the US mainland. USDA graphic. Photo Credit

http://bigislandnow.com/2014/01/16/

Figure 2: A Woman Using SODIS

(Swiss Federal Institute)

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can be treated at one time. The focus of Phase I was to explore different vessel configurations to

increase the quantity of water produced, and lessen the time it takes to disinfect the water.

Summary of Findings: Using a standardized laboratory

greywater solution (synthetic greywater) that was capable

of maintaining the viability of the E. coli, students

‘spiked’ synthetic greywater and divided it into two tubes:

1.) A solar UV (ultraviolet) transmitting tube and 2.) A

darkened tube of the same material which allowed no

penetration of light, as the control. This approach allowed

students to evaluate the effectiveness of the solar

disinfection prototype vessels and solar collectors. The

use of a standardized laboratory greywater solution

helped to ensure valid analysis of experimental results to

compare the effectiveness of greywater disinfection

between different experimental runs. The Biotechnology

team also worked on culturing a strain of E. coli that is

more resistant to the chemicals normally found in

greywater to simulate the type of E. coli bacteria that is

more commonly found in a residential setting. This hardy

strain of E. coli would serve to improve efficacy of solar

disinfection prototype designs. A comprehensive report of

findings and outputs regarding standardizing laboratory

greywater, culture of a chemically resistant strain of E. coli and final testing procedures,

protocols and analysis are further discussed in the project summary with supporting data and

graphs.

The Design Optimization and Fabrication team was comprised of students with various

disciplines of engineering, math, physics, and environmental majors. At the writing of this report

the Design Optimization team has developed three prototypes for testing standardized (synthetic)

greywater. A one-half-liter (0.5L) borosilicate glass tube with a high reflective parabolic trough

was tested and yielded adequate disinfection, with a 4-log reduction (99.99%) of E. coli cell

viability. A comparison study between borosilicate

glass and UV transmissive acrylic was performed

using a three liter Acryrite® tube and a three liter

borosilicate glass tube with a 3-fold wider diameter.

Again, test analysis demonstrated adequate

disinfection with a 4-log reduction in E. coli cell

viability and indicated a negligible difference in

efficiency of disinfection between borosilicate glass

and Acrylite® brand acrylic. In Phase I, design

Figure 3: ACC S-STEM student Joe

Rodriguez

…4-log

reduction

(99.99%)

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prototypes progressively expanded vessel size while focusing on

modifying reflective accoutrement to decrease the time it takes to

achieve disinfection.

ACC students hope to complete two additional experiments before

the National Sustainable Expo in April. Experiment 4 will be a

comparative study of solar collector design and the effect of

reflective intensity of light based on the design of the solar collector

in Prototype 1. Both the reflectivity of the material and the

parabolic shape of the reflector will be compared. Experiment 5 will be an evaluation of the

expansion of the test vessels and optimal solar collector design determined from Experiment 4.

Students project that the new prototype designs will demonstrate a substantial increase in volume

capacity of the disinfection vessel while significantly reducing disinfection time. Data, Findings,

Outputs/Outcomes are detailed in the project summary with supporting photos of prototypes

designs. SOP’s for the use of Prototype designs are published online and referenced at the end of

this report.

Conclusions: Austin Community College students have

proven the concept of applying the SODIS solar disinfection

approach towards the sanitation of greywater, as described in

the project The Evaluation and Expansion of the Solar

Disinfection Method of Reclaimed Residential Greywater.

Solar disinfection of water can be achieved at larger volumes

and at a more rapid disinfection rate than outlined by the

SODIS method of disinfection used for drinking water. The

use of solar disinfection can be very effective in the

residential setting and has the potential to save the United

State billions of gallons of water per year. Solar power, which

is the energy source on which solar disinfection operates, is

less energy-consuming in comparison to energy costs required

to operate other disinfection systems such as ozonolysis. Solar

disinfection can also reduce the need for toxic chemicals such

as chlorine in residential greywater disinfection processes.

This will lessen water disinfection byproducts from the environment.

Proposed Phase II Objectives and Strategies:

A. Continuation of current research models focused on the expansion of the volume of water

that test vessels can successfully treat and the reduction of exposure time to achieve

disinfection.

Figure 4: ACC S-STEM students perform

testing on solar disinfection prototypes

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B. In addition to expanding the volume and improving the efficiency of our solar system, we

would like to develop a fully-operational prototype that is ready for residential household

use. Further study is needed to determine the design of:

a. Pre- disinfection storage tanks

b. Pre- and post-treatment/filtration

c. The configuration needed to maximize gravity feed of the treatment system

d. The operation control design for automating the filling and emptying of vessels in

response to light intensity.

e. A dye that will photo bleach at the same rate of ultra-violet disinfection. This dye

would be environmentally friendly, and by use of a comparator, the homeowner

would be able to tell if greywater has been sufficiently treated.

f. The design must not only be aesthetically pleasing to a homeowner, it must be robust

enough that it can operate with a minimum of maintenance.

C. The S-STEM team would also like to expand our large-volume and rapid method of solar

disinfection into other applications such as in sanitation of rainwater collection systems

for drinking water. We hope to partner with Sustainacycle of Kyle, Texas who has an

interest in being able to commercialize our design product to replace the conventional

treatment methods currently used for sanitizing rainwater.

D. We feel that consumers using greywater treatment systems will not only become more

aware of their water usage, but will become more concerned about their contribution of

ecologically disruptive components into their environment. We hope to bring awareness

to the consumer of the impact that their selections of cleaning products and detergents

have on the quality of the water being treated by our solar disinfection system. In an

effort to promote educated consumer choices, we hope to test and compare greywater

recipes that use “environmentally friendly” detergents with recipes of “environmentally-

unfriendly” consumer products. These synthetic greywaters will be evaluated both

chemically and biologically.

Supplemental Keywords: Water, physical and biological integrity of the systems,

improvements in water purification and distribution, water conservation, sustainable water

management, urban water planning, water

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A. Summary of Phase I Results

Background and Problem Definition

Water is defined as a renewable resource; however, due to the exponential growth in human

population and subsequent increase in water demand, the availability of sustainable freshwater

sources is becoming increasingly limited (David Pimentel J. H., 1997). This water scarcity,

coupled with mankind’s climate vulnerabilities, has created water deficits affecting all levels of

society (Gleick, 2000). Addressing these hypercritical issues of water scarcity and water demand

necessitates the development of efficient water reuse technologies and more sustainable water

management practices (USGCRP, 2009). During Phase I, this project has focused on two

principle paths towards combing key applications to address this need for more sustainable water

practices: 1. Greywater recycling, 2. Incorporation of the solar, chemical free disinfection

processes. Both of these applications are in accord with current EPA and NSF guidelines, and are

expected to be an integral to future water management plans (EPA Region IX, 1998).

Given the historical dependent relationship between society and the availability of usable water,

the problems of water scarcity are at the crux of all growth and prosperity (Homer-Dixon, 1991).

By increasing the productivity of one unit of water, the results cascade across all sectors affected

by water scarcity; public health, the industries of energy and food production, as well as issues of

civil stability and security (Homer-Dixon, 1991) (David Pimentel J. H., 1997) (Gleick, 2000).

Historically, the benefits of creating more sustainable water use practices have had a positive

exponential effect, where access to water has always translated to prosperity. Without it,

populations are subject to disease, famine, war and collapse. (David Pimentel J. H., 1997).

In relation to people, prosperity and planet, the supplemental application of SODIS establishes

more efficient water use practices and conservation by:

Integrating water reuse / recycling

Dramatically reducing the amount of water each household will demand from their

municipal supplier, or well.

Reduction of potential DBP’s inadvertently created through chlorination.

While the issues of water scarcity and water demand are not new, recent stressor drought events

coupled with future population estimates have generated increased scrutiny into the efficacy of

current water management practices. With drought and other adverse climate events growing in

frequency, it’s becoming ever more important to increase the productivity of one unit of useable

water, while also reducing cost. When current trends of water use models are clear, combined

with expected population estimates, water scarcity will increasingly be defined by the

availability of sustainable water sources. At current levels of water use, increases in global

population will exponentially raise water demand beyond projected capacity. Given the

population increases expected worldwide, from 6.1B in 2000, to just under 10B by 2050, the

acquisition of sustainable water sources, will continue to be at the forefront of any responsible

water budget (EPA, 2010) (U.S. Census Bureau, 2011). There are no indications that water

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demand per capita will decline. Local solutions at point of use, such as water use efficiency and

water reuse, or recycling, “can satisfy most water demands, as long as it’s treated to ensure water

quality appropriate for use” (EPA Region IX, 1998). Incorporating greywater reuse at point of

primary use assuredly reduce demand upon municipal providers, while also empowering

individuals to institute more efficient water use behaviors.

By utilizing solar disinfection and greywater reuse for residential applications, water and

energy demand can be reduced, with substantial financial and water resource savings acquired.

Using a study of 1,188 homes, the 1999 American Water Works Association report, Residential

End Uses of Water, established a national household mean daily use of 409 gallons of water per

day (gpd), where 70 percent was used indoors, and 30 percent was used outdoors (Peter W.

Mayer and William B, 1999). Nationwide the indoor use remains relatively steady at 26.7

percent, with 76.44gpd going towards toilet flushing. As residential greywater represents all

waste water generated by a household excluding the toilet, a greywater reuse system could

recover an average of 209.86gpd. This volume covers the mean 199.14gpd of residential water

demand required for toilet and irrigation, which could be sourced by greywater reuse instead of a

municipal water source. That’s an average of 76 thousand gallons a year, per household (Peter

W. Mayer and William B, 1999).

Phase I also begins to illustrate some of the other benefits of the passive disinfection process

beyond volume. While conventional disinfection methods of chlorination, UV light bulbs, and

ozone all require extensive amounts of energy to perform, SODIS, which uses the sun as its

source of energy, does not. Therefore the benefits of SODIS go beyond reducing water demand,

while also lowering the energy and chemical demand required by the generation of potable

municipal water. Also, there are over 500 known disinfection by-products (DBPs )created

through chlorination, some of which have been found to have carcinogenic properties (EPA,

2012) (PCP, 2008-2009). Supplemental use of solar disinfection would, by substitution, lower

the detrimental health risks associated with DBPs (PCP, 2008-2009).

Purpose, Objective and Scope

Purpose: To expand established known limitations of the solar disinfection method as applied

to residential greywater. The water being treated is equivalent to greywater of a single family

home and is ideally representative of all the greywater which would be collected from showers,

bathtubs, hand washing lavatories, clothes washing machines, etc. Our approach for testing will

be microbial analysis of greywater, before, during, and after solar disinfection treatments. The

total microbial loading will be tested following procedures outlined in the National Primary

Drinking Water Regulations (EPA, 2009) and Standard Methods for the Examination of Water

and Wastewater (2012).

Data, Findings, Outputs/Outcomes

The research team’s primary focus for Phase 1 was to improve time efficacy, and push volume

restrictions of the solar disinfection method of residential greywater.

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Phase I Objectives and Predictions from Original Proposal

1. Objective 1: Fabricate disinfection vessels which replicate current capacity constraints of

the SODIS solar disinfection method.

Actual Accomplishment: ACC S-STEM students satisfied Objective 1 to fabricate

vessels which replicate current capacity constraints of the SODIS method. Students

performed a comparative analysis on two test vessel materials: acrylic and borosilicate

glass. Both test vessels met and exceeded current capacity constraints of SODIS.

2. Objective 2: Assess data after the first round of tests is conducted for each design;

conduct instructor consultations and incorporate their feedback, and; consider design

changes and the possible elimination of the weakest performing design.

Actual Accomplishment: ACC S-STEM students satisfied criteria outlined in Objective

2. Five vessel prototype designs were developed, and as of the writing of this report,

testing and experiments were performed on three. Additionally, five solar collector

designs were developed and fabricated. Comparative observations were made. As of the

writing of this report, a comparative experiment has been scheduled. Data and analysis

will be presented at the National Design Expo in April, 2015.

3. Objective 3: Make preparations for multiple assays to be run on the remaining vessel

designs for consistency (the final run of observations is intended to illuminate poorer

designs).

Actual Accomplishment: ACC S-STEM students satisfied criteria outlined in Objective

3. To date, each prototype design has the capacity to be ‘scaled up’ and expanded to

accommodate a larger volume of water without the use of energy or chemicals and with a

significant shorter exposure time than outlined in the SODIS standard.

Below are the summary reports that detail how objectives specified in the original proposal were

addressed and the outcomes/outputs that were successfully achieved.

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Biotechnology

ACC S-STEM students focused efforts to develop a standardized synthetic greywater that could

maintain cell viability of laboratory E. coli bacteria. Students developed a recipe for synthetic

greywater by combining a standardized bath-greywater recipe that was published in a Clemson

University study with a laundry greywater that was compliant to ANSI-NSF standards.

(Christopher, D. 2012)(NSF. 2013). The final recipe incorporated the most common and popular

household brands of cleaning products and detergents sold in America for each ingredient listed

(Lilly, K. 2014). These recipes were made in concentrate and then combined and diluted as

needed to form the final useable greywater. The full recipe is detailed on the attached “Preparing

1 L of 10x concentrated “mixed-use” and “Laundry” greywater” SOP.

To improve clarity of synthetic greywater that meets the criteria outlined in the SODIS method

with turbidity level less than 30 Nephelometric Turbidity Units (NTU)(Anwendung. 2009).

Synthetic greywater solution was filtered through microfiber clothes. Filtration is a necessary

step for effective solar disinfection because any particulate matter can act as a shield against the

UV rays (Anwendung. 2009). Without filtration the time it takes for ultraviolet radiation to

disinfect the water will increase or not work at all if the particulate matter concentration is too

high. Since the end-user of a residential greywater treatment system might want to be able to

access easily-obtained filtration materials, microfiber cloth products were explored for their

ability to reduce greywater turbidity to the SODIS-specified 30 NTU levels. Microfiber cloths

sold for household cleaning was found to fit this requirement.

Escherichia coli (ATTC 25922) was used to test for solar disinfection, since this is only

microbe currently regulated by the City of Austin, Texas. Students observed that the synthetic

greywater solution had issues with maintaining cell viability when inoculated with low levels of

Escherichia coli. To remedy this, tests were performed to optimize the E. coli inoculation sizes

used for testing over the 6-hour period of solar exposure. A 2% inoculum was found to provide

a low background level of cell death following inoculation. Moreover, students adapted a strain

of E. coli that was more resistant to the chemicals synthesized in the greywater by routinely

subculturing this strain in 20% greywater. The final results showed that a 2.0% inoculation of E.

coli that survived the previous experiments was a good option for the testing of solar disinfection

of greywater. The final solution used in all subsequent testing was therefore a 50:50 mix of

laundry to bath greywater solutions inoculated with 2.0% E. coli.

The standard protocol for preparing the greywater-resistant E. coli for inoculation was as

follows. On the first day, an isolated E. coli colony is selected from a media plate and transferred

to a culture tube containing 10% filtered sterilized greywater and 90% tryptic soy broth (TSB).

The culture was shaken overnight at 32ºC at 180 rpm. During the second day the culture was

transferred to new culture tubes containing 20% filtered sterilized greywater and 80% TSB and

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were shaken overnight at 32ºC at 180 rpm. On the final day the culture was transferred to a final

flask containing the amount of 20:80 greywater-TSB needed to achieve a 2% inoculation.

Optimal amount of greywater for culturing was devised by using a series of different greywater

to TSB concentrations at 2% E. coli inoculations with the A600 measured daily.

Experiment Data, Graphs and Analysis

Figure 5: Solar Disinfection results for 10% E.coli greywater

Results and Analysis of Experiment 1: The 0.5L borosilicate glass vessel in conjunction with the

12” parabolic trough high reflective solar collector in this experiment produced a 4 log reduction

in CFU/mL of E. Coli. (99%). The disinfection of Prototype 1 was more successful than the 3 L

and 0.5 L PET bottles that placed on a reflective surface according to the standard SODIS

method, which did not produce any decrease in CFU/mL when streaked. This first solar

prototype was used, along with a 3 L bottle control, to compare results from experimental runs

that followed, and the same pattern was observed under all circumstances: a rapid decline in E.

coli viability in the prototype, with little loss in dark controls, and substantially less loss of

viability in the light-treated 3L bottle.

Unfortunately, the 10% E. coli inoculum created a high turbidity in the greywater, resulting in a

2-log (99% disinfection) over a period of 6 hours in the sunlight.

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Experiment 2:

Results and Analysis of Experiment 2: Experiment 2 tested a lower inoculation size of E. coli:

1% (v/v) in synthetic greywater. Unfortunately, this inoculatin rate resulted in a drastically-low

viability in the dark controls. Subsequent lab studies showed that pre-conditioning the E. coli

innocula in 20% filter-sterilized greywater in TSB and increasing the inoculation rate to 2% (v/v)

E. coli resulted in a more robust maintenance of cell viability in the dark.

Experiment 3:

Results and Analysis of Experiment 3: For Experiment 3, biotech students cultured a 2.0%

inoculation of greywater resistant E. coli in accordance from data analysis of experiment 2 and

intervening lab studies. The vessels were filled immediately after inoculation. The graph implies

a number of things. There is approximately a 90 minute time period observed for the E. coli to

acclimate to its environment, which is seen in both the initial dip and spike before the samples

even out. Also, the control samples not exposed to UV now either even out or continue to grow.

This implies that the E. coli samples are now able to live in the greywater and that it is the UV

treatment disinfection the water. Note, the original prototype of the 0.5 liter tube never has an

initial spike in E. coli growth. This suggests that the diameter of the test vessel may be one of

the most important factors of the effectiveness of UV treatment of greywater.

Figure 4: Solar Disinfection results for 2.0% E.coli greywater

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This experiment was performed in near freezing temperatures on a very cold and windy day in

Texas, compared to the previous experimental runs. The log-6 (99.9999%) loss in E. coli

viability and a log-4 (99.99%) loss in the wider-diameter Prototype 2 vessels within 4 hours of

sunlight such sub-optimal conditions was very encouraging. This run also demonstrates that

ultraviolet radiation alone, without a synergistic effect of heating, can efficiently kill the

bacteria.

A more stable

initial culture

density might be

result from pre-

incubating the

synthetic

greywater for a

couple of hours

prior to loading

the

photobioreactor

prototypes in

subsequent

experimental

runs. This may

lead to results that

are easier to interpret and to estimate how long it takes for the UV treatment to begin working.

Also, although the initial growth spike appears to be higher in glass than in acrylic, the amount

of time t to reach a 99% kill rate is approximately the same. The glass material appeared to

weather the elements and handling better, as the acrylic material quickly sustained several

scratches of unknown origin. This knowledge, combined with the sustainability factors of glass

vs acrylic, has led to a decision to discontinue use of expensive acrylic models for more eco-

friendly and inexpensive glass models.

There are two additional experiments planned after the submission of this report. The first one is

scheduled for Friday, March 13, in which comparisons will be made between the shape of the

parabolic solar collectors and the intensity of the reflective linings. The goal of this experiment

will be to standardize the shape and the reflective surface of all experiments in the future. The

second experiment will be on Friday, March 27 and will involve upscaling of the current models

to larger vessel size. The purpose of this experiment will be to test the increase in diameter of

test vessels. During this experiment two vessels of different diameter of borosilicate glass will be

compared to determine if future models need to focus on larger volume vessels or on multiple

Figure 5: Temperature Time Series Experiment 3

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thinner vessels connected using modular assembly methods. The effects of increased vessel

diameters and relative sizing of the solar collectors remain to be examined, but is on the agenda

for completing this research project.

Design Optimization and Fabrication

Prototype 1

Borosilicate glass was chosen as the material for Test

Vessel 1. Borosilicate glass is a type of glass with silica

and boron trioxide as the main glass-forming constituents.

Borosilicate glass has a low coefficient of thermal

expansion (~3 × 10−6 /°C at 20 °C), making it resistant to

thermal shock (Borosilicate Glass. (2005). Additionally,

borosilicate glass allows for UV-A (wavelength 320–400

nm) transmission (Varnakavi, N. 2012), making it the

optimal choice of material for the vessels in The

Evaluation and Expansion of the Solar Disinfection

Method of Residential Greywater project. UV-A solar irradiation can inactivate water borne

microorganisms therefore, selection of a material for test vessels that allows for this wavelength

of the ultraviolet spectrum is necessary to achieve optimal results. Test vessel 1 had an outside

diameter of 32.6mm and tube length of 1053mm. The borosilicate glass tube was suspended in a

semi-circular trough (solar collector) with a high-reflective lining of aluminum. The Design

Optimization and Fabrication team used a schedule 40 pvc pipe that was sliced horizontally to

fabricate the solar collector. The shiny side of aluminum foil was used as the lining to make the

interior surface of the solar collector reflective. The width of the solar collector was 6 inches.

The borosilicate glass tube was suspended inside of the solar collector with small pieces of

acrylic to maximize the amount of ultraviolet light penetration. The point at which solar rays

meet after reflection is known as the focal point. This point can be found in a semi-circular

reflective trough with the equation , with r being the radius of the trough.

The frame of the solar collector and test vessel, also referred to as the ‘cradle’, was constructed

out of typical A36 carbon steel and was welded to create a sturdy frame. The frame design was

constructed so that the solar collector would be at the correct geographic latitudinal angle to the

sun, to maximize solar efficiency. Austin, Texas is located at 30.2500° N, so the correct angle for

the solar collector would be 30 degrees.

Figure 6: Prototype 1

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It was recommended that the next experiment involve duplicate versions of every model being

tested with one of each model not being exposed to light. Because of time constraints and

necessity to generate data, the Design Optimization and Fabrication team chose to expand

volume size of the test vessels and also agreed with the Biotech team that more controls were

needed for the next experiment to justify results. Additionally, The Design Optimization and

Fabrication team wanted to determine if borosilicate glass was truly the best material to achieve

maximum results in solar disinfection of greywater. It was decided that Experiment 2 would also

be a simultaneous comparative study and analysis of borosilicate glass to Acrylite® brand acrylic

tubing.

Prototypes 2-A & 2-G

Prototypes 2-A and 2-G were essentially the same design, however the test vessels were of

different materials; Borosilicate glass and Acrylite® brand acrylic. Prototype 2-G consisted of a

test vessel made of borosilicate glass. It was determined that the volume of greywater for

Experiment 2 would be increased to 3L. The borosilicate glass test vessel for Prototype 2-G had

an outside diameter of 80mm and a length of 882mm. The Acrylite® brand acrylic test vessel

for Prototype 2-A had an outside diameter of 110mm and a length of 1500mm.

Figure 7 Prototypes 2 and 3 Borosilicate Glass

And Acrylic Tube Test Vessels

Analysis of Biotech Report: Data suggests that there is a correlation in the diameter of each

vessel and the initial spike of E. coli during experiment. Biotech students hypothesize that once

the diameter of the disinfection vessel reaches a threshold, it takes longer for solar disinfection to

begin working. One of the objectives of the Design Optimization and Fabrication team is to

determine the threshold diameter at which solar disinfection becomes ineffective. This diameter

threshold will take into account the focal point of the solar collector to maximize UV-A

penetration. The comparison analysis of borosilicate glass vs. UV transmissive Acrylite®

acrylic was found to be negligible. Given the petroleum based manufacturing methods of acrylic,

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the Design Optimization and Fabrication team has opted to use eco-friendly borosilicate glass for

future studies.

Experiment 4 is scheduled for March 13, 2015. It will consist of 4 prototypes of 0.5L borosilicate

test vessels for each solar collector design. Data, Graphs and comparative analysis are pending

and will be available for presentation at the P3 National Sustainable Design Expo in Washington.

SOP for Prototypes 1, 2-A & 2-G can be found here. (Lilly, K. 2015).

Discussion, Conclusions, Recommendations

We postulate the evaluation and expansion of the solar disinfection of water will yield benefits

worldwide across all demographics of economic standing. The combined reduction of water

demand and increased productivity of a single unit of water will open opportunity to further

human growth, provide stability in public health and usher in fundamental changes to water

practices of consumer end-users. Current modern systems of water management are inefficient.

They waste water, energy, and money by not matching the quality of water to its applied use.

Appropriately matching water-quality to water-need will allow for the reuse of greywater to

become a more accepted process, where the use of potable water in non-potable applications like

toilet flushing and landscaping to become obsolete. As a key strategy for reducing demand, the

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implementation of the solar disinfection of greywater is an important strategy and will help to

provide more sustainable water management practices. This study proves that inexpensive

materials can be configured to safely, rapidly, and efficiently disinfect greywater. Depending on

diameter sizes of the greywater vessel, the sizes of the solar collector, and the reflectivities of the

solar harvesting surfaces, a greater than 4-log (99.99%) loss in viability of E. coli in less than 4

hours can be achieved. This kill-rate meets regulated standards for E. coli loads, based on

bacterial loadings of household greywater being reported in the literature. The advantages of

low energy-intensity and low-toxicity of this treatment system shows promise for use in the

marketplace for reuse of household greywater for residential irrigation of landscapes and

gardens.

To the betterment of both human prosperity and the planet, the benefits of the solar disinfection

method of greywater spread beyond economic concerns and into environmental and qualitative

value by reducing the demand for the conventional disinfection processes currently required by

the production of high-quality potable water. The supplemental application of the solar

disinfection method of greywater will not eliminate the use of chlorination; however, the

subsequent reduction in chemical demand will lessen the inadvertent introduction of DBP’s into

the environment.

Recommendations:

Better public information and awareness of the opportunities, benefits and risks associated with

greywater will be necessary to expand greywater reuse.

Further study is recommended into the long term effects of conventional cleaning products in use

with a residential greywater irrigation systems.

Assurance that research misconduct has not occurred during the reporting period In an effort to expand scientific knowledge, improve the public-well being and to conserve

limited resources, the 2014-2015 Austin Community College S-STEM research team has

accepted federal funding in good faith, to pursue the call of scientific query. We affirm our

process has been transparent, impartial and conducted with the highest integrity and ethical

considerations. The ACC S-STEM research team is committed to responsible and ethical

research conduct and practices, and assures that no research misconduct has occurred during the

reporting period to include but not limited to: fabrication, falsification, or plagiarism in

proposing, performing, or reviewing research, or in reporting research results.

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B. Proposal for Phase II

P3 Phase II Project Description

Project Description, Novelty and Evaluation

The expansion of the solar disinfection method of residential greywater can have a significant

impact on water consumption and conservation efforts here in the United States. This technique

can be applied to many areas spanning the full range of social and economic demographics. As

this technology was initially developed in parts of the world where drinking water is scarce,

(Simon Dejung, M. W. 2007) new developments can only create additional prospects for

greywater reuse. The research team hopes to show that with proper disinfection, the collection

sources and reuse options for collected residential greywater can be expanded. If successful in

creating an energy and chemical free method of disinfecting residential greywater, the costs of

collecting, treating and reusing this reclaimed water will reduce. In turn, the average household’s

demand from aquifers, surface water, and local municipal sources will lessen, and will ideally

expand EPA and state codes regarding collection/reuse (Simon Dejung, 2007). Current

residential greywater treatment and disinfection systems can be supplemented and improved by

our expanded solar disinfection research and successful prototypes and designs, which will

reduce energy and chemical demand. Ultimately, providing improvements in water reuse for the

home will greatly reduce overall water waste.

Proposed Phase II Objectives and Strategies:

A. Continuation of current research models focused on the expansion of the volume of water

that test vessels can successfully treat and the reduction of exposure time to achieve

disinfection.

B. In addition to expanding the volume and improving the efficiency of our solar system, we

would like to develop a fully-operational prototype that is ready for residential household

use. Further study is needed to determine the design of:

a. Pre- disinfection storage tanks

b. Pre- and post-treatment/filtration

c. The configuration needed to maximize gravity feed of the treatment system

d. Irrigation systems that are best compatible with solar disinfection and volume of

treated water

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e. The operation control design for automating the filling and emptying of vessels in

response to light intensity.

f. A dye that will photo bleach at the same rate of ultra-violet disinfection. This dye

would be environmentally friendly, and by use of a comparator, the homeowner

would be able to tell if greywater has been sufficiently treated.

g. Finally, the design must not only be aesthetically pleasing to a homeowner, it

must be robust enough that it can operate with a minimum of maintenance. We

hope to partner with local companies in the area who support sustainability.

h. The S-STEM team would also like to expand our large-volume and rapid method

of solar disinfection into other applications such as in sanitation of rainwater

collection systems for drinking water. We hope to partner with local companies in

the area who support sustainability who has also have an interest in being able to

commercialize our design product to replace energy consumptive and chemical

dependent treatments currently in use for sanitizing rainwater.

C. Consumers using greywater treatment systems will likely not only become more aware of

their water usage, but will also become more concerned about their contribution of

ecologically disruptive components into their environment. We hope to bring awareness

to the consumer of the impact that their selections of cleaning products and detergents

have on the quality of the water being treated by our solar disinfection system. In an

effort to promote educated consumer choices, we hope to test and compare greywater

recipes that use “environmentally friendly” detergents with recipes of “environmentally-

unfriendly” consumer products. These synthetic greywaters will be evaluated both

chemically and biologically.

The Public Health and Safety Organization and the National Science Foundation have published

guidelines (NSF/ANSI Standard 350 and 350-1) to establish material, design, construction and

performance requirements for onsite residential and commercial water reuse treatment systems.

They have also set water quality requirements for the reduction of chemical and microbiological

contaminants for non-potable water use. S-STEM students will strictly adhere to these guidelines

and parameters set forth by these agencies and supported by the E.P.A. (Environmental

Protection Agency) to ensure the safety of public health. Greywater treated by prototype designs

for solar disinfection will also meet the Standard 350 effluent criteria in order to sustain the

natural resources of the planet and will additionally utilize F.D.A. (Food and Drug

Administration U.S.) approved materials in the construction of all prototypes and storage

equipment and tanks. Following these guidelines will provide a consistent method and measure

of performance and compliance for the final design of the residential greywater solar disinfection

unit.

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Quality Assurance Statement ACC S-STEM students are strongly committed to the application of sound scientific principles in

its analyses, and the production of quality and environmentally protective prototypes with sound

engineering design in the Evaluation and Expansion of the Solar Disinfection Method of

Residential Greywater. All data collection methods will adhere to procedures outlined in the

EPA Requirements for Quality Assurance Project Plans EPA QA/R-5 (EPA, 2001). All sample

designs will adhere to procedures outlined in the EPA Guidance on Choosing a Sampling Design

for Environmental Data Collection EPA QA/G-5S (EPA, 2002).

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Project Schedule Months 1 – 6 (August 2015 – January 2016)

Ordering of all supplies, equipment and tools listed for Year 1 on Budget Justification worksheet.

Continue research, design and expansion of solar disinfection test vessels and collectors until

disinfection of a 25 gallon vessel is achieved.

Design Optimization and Fabrication team will develop 2 modular design concepts for a ‘turn-

key’ residential system.

Automation team will design concept ideas for the automated/operational filling and emptying of

test vessels that can be immediately utilized in scaled up prototypes for testing and analysis.

Biotech team will continue to test and analyze data from test vessels to determine if disinfection

of residential greywater has been achieved and to make recommendations on parameters that

need to be adjusted in solar disinfection design to improve volume constraints and decrease

disinfection time.

Biotech team will begin research, testing and analysis of a photo bleaching dye that will facilitate

end consumer in determining if disinfection of residential greywater has been achieved.

Months 6 – 12 (February 2016 – July 2016)

Design Optimization and Fabrication team will begin to build out first modular disinfection

system capable of processing a volume of 25 gallons of residential greywater per day.

Automation team will integrate automated/operational design so that modular disinfection system

can operate without user control.

Biotech team will continue to test and analyze data from solar disinfection systems to determine

system’s capabilities to achieve disinfection of residential greywater. The biotech team will

continue to make recommendations on parameters that need to be adjusted in solar disinfection

design to improve volume constraints and to decrease disinfection time.

Biotech team will continue research efforts in developing a photo bleaching dye and will test

developed products in lab and in field.

Months 12 – 18 (August 2016 – January 2017)

Ordering of all supplies, equipment and tools listed for Year 2 on Budget Justification worksheet.

Design Optimization and Fabrication team will begin to build out second modular disinfection

system capable of processing of volume minimum of 25 gallons of residential greywater per day

based on success/failure analysis of first modular disinfection system that can be utilized

anywhere in the contiguous United States based on geographic latitude.

A comparative analysis will be performed testing the durability, lifecycle and weight loads

between borosilicate glass and acrylic for larger scaled residential systems.

Fabrication team will develop a solar disinfection system capable of being mass produced.

CAD (Computer Animated Drawing) schematics will be designed and a solar disinfection unit

base will be laser cut out of foam.

A wax mold will be constructed for a fiberglass base that can be mass produced.

Fiberglass base will be integrated into second modular disinfection system.

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Biotech team will continue to test and analyze data from solar disinfection systems capabilities to

achieve disinfection of residential greywater, and continue to make recommendations on

parameters that need to be adjusted in solar disinfection design to improve volume constraints and

to decrease disinfection time.

Biotech team will continue research efforts in developing a photo bleaching dye and will test

developed products in lab and in field.

Biotech team will begin comparative analysis of greywater recipes that are environmentally

friendly against recipes of conventional and popular environmentally “unfriendly” products.

These synthetic greywaters will be evaluated both chemically and biologically. Results will be

published.

Months 18 – 24 (February 2017 – July 2017)

Team final and official reports on all prototype designs (biologic and engineering), data, findings,

outcomes/outputs, presentations, and partnerships will be generated with accompanying

schematics, SOP’s, graphical data and publications.

Teams will prepare to present in Washington at the National Sustainable Design Expo in April.

EPA Human Subjects Research Statement The proposed research does not involve human subjects.” All research is being conducted on

types of SODIS disinfection vessels created by Biotechnology and Environmental Science

students enrolled at Austin Community College District (ACC).

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C. References

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Enforcement and Compliance Assurance (2201A). Retrieved from

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EPA. (2012, March 06). Water: Microbial & Disinfection Byproducts Rules. Retrieved 03 15, 2012, from EPA:

http://water.epa.gov/lawsregs/rulesregs/sdwa/mdbp/chloramines_index.cfm

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EPA Region IX. (1998). Water Recycling and Reuse: The Environmental Benefits. 909-F-98-001. Washington DC:

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Lilly, K. (2014). Popular Market Items. Retrieved from docs.google.com:

https://docs.google.com/document/d/1Xfb6jqlT5z_hTyMzRKB_VCijLEh4k9fbNSEHJTiHdgU/pub

Lilly, K. (2015). SOP for SODIS Prototypes 1, 2-A & 2-G. Retrieved from docs.google.com:

https://docs.google.com/a/g.austincc.edu/document/d/1FJrwgaee11v8ofy6KkJYWCwUHHS-ynL-

at6PbNIQ0Ks/pub

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U.S. Census Bureau. (2011, June NA). International Programs. Retrieved from United States Census Bureau:

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Attachments

Preparation of 1 L of 10x concentrated “mixed-use” and

“Laundry” greywater

Title: Preparing 1 L of 10x concentrated “mixed-use” and “Laundry” greywater

Institution: Austin Community college

Prepared by: Revision Number: 002

Scope and Application

The purpose of this SOP is to give instruction in the creation of 1 liter of concentrated synthetic greywater

Summary of Method

Dissolve substances in minimal Deionized water and stir to dissolve. Then use deionized water to bring each solution to a final volume of 1 L. When needed mix solutions 50:50 and add 18 liters of tap water for a final volume of 20 liters.

Materials Required

Mixed Use Greywater

Colgate Fluoride – Regular Gillette Endurance

Test Dust Commet with Bleach

Head and Shoulders 2 in 1

Dove Shea Butter Softsoat, Antibacterial Deionized (DI) water

Laundry Greywater

All (2x) or Tide (2x) NaHCO3

Na2PO4

(NH4)2SO4

Equipment Required

1 L Beaker Graduated Cylinders

Funnels

pH meter and buffers with HCl and NaOH

Stir Bars and Plates

Weight Boats

Container that can be autoclaved

Page 29: Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed Residential Greywater

Protocol

Protocol Experimental Data

Mixed Use Greywater

1. Measure out the following ingredients and place them into a beaker containing 0.5 L of DI water

a. 0.30g Colgate Fluoride Regular b. 0.20 Gillette Endurance c. 1.00g Test Dust d. 1.90g Head and Shoulders 2 in 1 e. 3.00g Dove Shea Butter f. 2.30g Softsoap, Antibacterial g. 1.00g Comet with Bleach h. 0.30g Lactic Acid

2. Stir to dissolve. Add more water if necessary but do not go over 1 liter.

3. Use a calibrated pH meter and HCl and NaOH to bring the solution to 7.4 pH

4. Transfer the contents of the beaker to a 1 L graduated cylinder and bring to volume with DI water.

5. Transfer to a labeled container and autoclave to sterilize.

6. Hold until ready to use.

Laundry Greywater

7. Measure out the following ingredients and place them into a beaker containing 0.5 L of DI water a. 4.00 mL All (2x) or Tide (2x) b. 0.20g NaHCO3 c. 0.40g Na2PO4 d. 0.40g (NH4)2PO4 8. Stir to dissolve. Add more water if necessary but do not go over 1 liter.

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9. Use a calibrated pH meter and HCl and NaOH to bring the solution to 7.4 pH

10. Transfer the contents of the beaker to a 1 L graduated cylinder and bring to volume with DI water.

11. Transfer to a labeled container and autoclave to sterilize.

12. Hold until ready to use.

Mixing Greywater For Use

1. Mix the “mixed use” and laundry greywater 50:50.

2. Add enough tap water so that the 50:50 greywater mix is 10% of the final volume. Show calculations on the right.

3. Use within 3 days.

Budget

The proposed budget for Phase II development of The Evaluation and Expansion of the Solar

Disinfection Method of Residential greywater is as follows:

Construction Supplies - $22,450.00

Custom Fiberglass Fabrication - $8,500.00

Automation and Robotic Supplies - $4,920.00

Analytical Products - $21,455.30

Travel - $10,504.00

Indirect Cost @ 10% - $6,782.93

Total - $74,612.23

The design of a residential ‘turn-key’ greywater disinfection system will require pre and post-

treated greywater storage, plumbing, fittings, and method/systems of treated water for

irrigational purposes. Operational/automation control designs will facilitate the filling and

emptying of treatment vessels and eliminate consumer operating error by automating correct

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geographic latitudinal angle. Automated sensors will also ensure public safety by ensuring that

an influx of influent greywater is re-directed to appropriate systems.

Finally, this system will need to be engineered and designed at a level that would support mass

production and consumer friendly installation and monitoring, while maintaining a physically

aesthetic and unobtrusive presence.

Instrument kits and analytical products will be used to monitor water quality and microbial

parameters in laboratory setting. These products will determine whether conditions are favorable

for microbial growth and/or if disinfection has been achieved in laboratory and field testing.

Reagents will help calibrate probes and provide standards for the colorimetric assays.

The Budget Justification is in accordance with OMB-approved form SF-424A is itemized in the

Budget Justification.

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Budget Justification

Budget Categories Year 1 Year 2 Total

Construction Supplies

Aluminum/Galvanized Tin Sheets and reflective materials $1,250.00 $1,250.00 $2,500.00

Hardware: Nuts, bolts, clamps, quick connect fittings, food grade gaskets, food grade hosing, food grade tubing, couplings, reducers and extenders, mounting hardware, spigots, shut-off valves, supply lines, screws, adhesive, mirrored film $500.00 $250.00 $750.00

Construction Tools: Propane torch, tongue and groove pliers, hacksaw, metal file, basin wrench, pipe wrench, hand auger, adjustable wrench, tubing cutter, blades, drill, drill bits, sanding material, wood clamps, hosing C-clamps, bracing and brackets $500.00 $500.00

FDA approved Water Storage Tanks - 25 gallons - 4 @ $500.00 each $2,000.00 $2,000.00

Borosilicate Glass Products (Custom order: minimum 0.5 ton melt glass) $2,500.00 $5,000.00 $7,500.00

Acrylite® acrylic tubes $500.00 $500.00 $1,000.00

Lumber (Structural systems support) $500.00 $500.00 $1,000.00

Warehouse Storage Rental @ $300.00 per month $3,600.00 $3,600.00 $7,200.00

Total $11,350.00 $11,100.00 $22,450.00

Custom Fiberglass Fabrication

CAD Laser cut foam mold $1,500.00 $1,500.00

Custom Wax mold Pour (Resin Coat, Seal, Wax and Tooling Gel, Laminate) $3,500.00 $3,500.00

Fiberglass pour (20 Units) $3,500.00 $3,500.00

Total $8,500.00 $8,500.00

Automation and Robotic Supplies

Microcontroller Board (Arduino and compatible) @ 75.00 each $150.00 $150.00 $300.00

Direct Drive (Pan and Tilt) SPT200 Direct Drive Pan & Tilt System @ $50.00 each $100.00 $100.00 $200.00

Water Sensor Kits (Temp/FLow) @ $50.00 each $100.00 $100.00 $200.00

DFRobot Wireless Programming Module for Arduino @ $110.00 each $220.00 $220.00 $440.00

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Digital Linear Actuator (2in) Heavy Duty 115 lbs. @ 250.00 each $500.00 $500.00 $1,000.00

Circuit Board - Snap Circuits Extreme 750-in-1 Kit w/Computer Interface @ $100.00 each $200.00 $200.00 $400.00

Tamiya Planetary Gear Box @ $20.00 each $40.00 $40.00 $80.00

Various Mechanical Parts (Spacers, bars, screws, gear mounts, motor mounts, acrylic enclosures for microcontrollers, cables, guide rails, bearings, clamps, adapters, tracks, sprockets, pulleys) $475.00 $475.00 $950.00

Laptop (for Programming) Toshiba 15.6" Satellite C55D-B5319 Laptop PC with AMD E1-2100 Processor, 4GB Memory, 500GB Hard Drive and Windows 8.1 $250.00 $250.00

400W solar power unit with 1500W power inverter and 12V battery for field-testing prototypes $1,000.00 $1,000.00

Educational Materials/ Necessary Software $100.00 $100.00

Total $3,135.00 $1,785.00 $4,920.00

Analytical Products

Reagent, supplies for mulitmeter (glassware, reagent kits, calibration kits), L-Spreaders $2,400.00 $2,400.00 $4,800.00

Microbiological Growth Media, TSA @ $350.00 each and TSB Powder , $700.00 $700.00 $1,400.00

Vernier LabQuest2 analytical instrument with Optical DO Probe, SpectraVis spectrophotometer/fluorimeter, CO2 sensor, temperature probe, pH meter, and software for field-testing prototypes @ $2700 per pkg $5,400.00 $5,400.00

Petri Dishes 35x10 mm, case @ $250.00 each $500.00 $500.00 $1,000.00

Plastic Cuvettes (UV-VIS) $200.00 $200.00 $400.00

Water Purification Filter DIY Kit Ceramic Carbon Silver Impregnated 4x4 in. @ $50.00 each $500.00

$800.00

$1,300.00

Portable Incubators/Cooler @ 679.00 each $1,358.00 $1,358.00

Escherichia coli lyophilized cells @ 245.00 each $490.00 $490.00 $980.00

Phosphate Buffered Saline @ $100.50 $201.00 $201.00 $402.00

Pipettes @ $119.70 each $239.40 $239.40 $478.80

Coliscan EZ-gel 10 sets or more - $21.05 per set $210.50 $210.50 $421.00

Eppendorf Easypet 3 (2) @ $458.00 $916.00 $916.00

Hannah Combo pH/EC/TDS/Temp Tester $199.50 $199.50

Analytical Standard Pigment @ ~$100.00 each $500.00 $500.00 $1,000.00

Organic Extraction Solvents $500.00 $500.00 $1,000.00

Solvent Safe Pipette tips $200.00 $200.00 $400.00

Page 34: Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed Residential Greywater

Total $14,514.40 $6,940.90 $21,455.30

Travel

Travel to the National Sustainable Design Expo: Hotel: 8 people at 200/night x 3 nights = $4800.00; Airfare: 8 people x $500.00 roundtrip = $4000.00; Per Diem: 8 people x $46/dy x 3 = $1104.00

$9,904.00 $9,904.00

Local Travel $600.00 $600.00

Total $10,504.00 $10,504.00

Total Direct Costs $67,829.30

Total Indirect Costs @ 10% $6,782.93

Total Grant Request $74,612.23

Relevance and Past Performance

Relevance to EPA

“Water research conducted at the EPA provides the science and tools necessary to develop

sustainable solutions to 21st century water resource problems, ensuring water quality and

availability in order to protect human and ecosystem health” (E.P.A. 2014. Water Research).

The research and development of The Evaluation and Expansion of Solar Disinfection Method to

Treat Residential Greywater affords a viable and economical solution to current water

availability issues that affect the majority of the U.S. today.

There is a growing demand for safe, reliable, and cost-effective reclaimed wastewater in the

U.S. (E.P.A. 2014. Water Research) The decrease of water demand and increased productivity

of a single unit of water, will provide insurance for future water availability and guide necessary

changes to consumer water practices spanning a full range of demographics.

Solar disinfection can also further the goals set forth by the E.P.A. of pollution control and

prevention by reducing the need for the use of chlorine in residential greywater disinfection

processes. This will reduce water disinfection byproducts from the environment.

Past Performance of the Principal Investigator:

There is no prior past performance information and/or reporting history that exists for principal

investigators, Dr. Patricia Phelps or Dr. George Staff.

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Resumes

Dr. Patricia Phelps

Kristine Lilly

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Student Bios

Ben Jeffries is a student at Austin Community College in Austin, Texas. Ben is

slated to graduate in 2016 with an A.A.S. in Environmental Science/Technology. He

currently works for the United States Geological Survey as a Hydrologic Technician

under the Department of Interior’s Student Career Pathways program. Honors and

Awards: Lead student investigator and author of the E.P.A. Project P3 - Grant: #SU

835926 Evaluation and Expansion of Solar Disinfection Method of Residential

Greywater. Current License held as C-Class Wastewater Operator TCEQ#

WW0044287. Participant: CCURI National Poster Session, Washington, D.C. Hart

Senate Building, Oct 2014. Awarded for his authorship and presentation of the pilot

study, Insect Biodiversity Loss Due To Automobile Impact. NSF Grant # NSF

1118679. He is devoted towards issues of water quality, watershed analysis and

environmental concerns. He currently volunteers for the USGS Green Team and stream cleanup events.

G.P. Selvaggio is a student at Austin Community College, in Austin, Texas.

G.P. moved to Texas from Los Angeles, California, and began study at ACC to

complete his Physics and Calculus sequences. He plans to transfer to the Cockrell

School of Engineering at the University of Texas in the fall of 2015. G.P. has

functioned as the Team Leader of Fabrication and Design Optimization for the EPA

P3 Project Grant: #SU 835926 Evaluation and Expansion of Solar Disinfection

Method of Residential Greywater. G.P. volunteers as an Algebra tutor for

underprivileged high-school students, and speaks at schools around Austin

regarding drug and alcohol awareness.

Kevin Strickland is a biotechnology student at Austin Community College

located in Austin, Texas. Kevin is currently focusing on various undergraduate research

projects before his expected graduation date in summer 2015. Kevin is currently the

lead of the biotechnology team of the EPA-sponsored undergraduate research project

involving the solar disinfection of greywater for reuse in residential irrigation. This

research will be presented at the EPA P3 National Sustainable Design Expo in

Washington D.C. later this year. Kevin plans to use his scientific background to gain

entrance to graduate school for Microbiology in the fall of 2016.

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Jered Staton is a second year student at Austin Community College (ACC) in

Austin, Tx. Jered will be transferring to a four year university in the fall of 2015 to

pursue a degree in Civil Engineering. Jered was awarded the S-STEM scholarship in

the fall of 2014 and is currently working with fellow students on a sustainability

project. As a former welder and materials enthusiast Jered was selected to join the

Optimization/Fabrication team. As a member of this team he has an opportunity to

offer his knowledge of materials and construction in order to build an ideal

prototype. Jered served as an officer in the United States Marine Corps prior to

returning to school in the pursuit of a second bachelor’s degree. Using skills

obtained through military experience Jered has been able to effectively manage his

team in order to meet all deadlines set forth by project administration. While not in

class or working on the project, Jered serves as an intern for the Texas Commission

of Environmental Quality’s Water Department and as a Math and Physics tutor at

ACC’s learning lab during weekend days.

Kristine Lilly is a student at Austin Community College located in Austin, Texas.

Kristine is majoring in Environmental Science and is slated to graduate in 2015 with

an A.S. and A.A.S. in Environmental Science/Technology. She currently works for

Austin Community College as the S - STEM Project Manager and Student

Coordinator for E.P.A. Project P3 - Grant: #SU 835926 Evaluation and Expansion of

Solar Disinfection Method of Residential Greywater. She has also worked as a tutor at

Austin Community College helping students in the areas of Geographic Information

Systems, College Algebra and Speech - Public Speaking. Honors and

Awards: President's Honor Roll 2011 - Present, Phi Theta Kappa Honor Society 2011

- Present, S.T.E.M. Scholarship Recipient 2012 - 2014 and Women's Independent

Scholarship Program Recipient (W.I.S.P.) 2011 - 2014. Kristine currently volunteers

for the LCRA River Watch program by providing water quality analysis on the

Colorado River monthly. Her work helps to ensure water quality along the Colorado

River in the State of Texas. She is a member of the Sierra Club - Lone Star Chapter

and the Coastal Conservation Association - Aransas Bay Chapter.

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Caitlyn Lankford is a full-time 3rd year student attending Austin Community

College (ACC) located in Austin, Texas. She will be graduating from ACC with an

Associate’s degree in Engineering in the fall of 2016. Caitlyn plans to continue her

education at a four year university where she will eventually work towards a Master’s

degree in Biomedical Engineering. She was awarded the opportunity to be a part of the

S-STEM scholarship project where she is currently a part of the Biotech team. Caitlyn is

also on the President’s Honor Roll at ACC where she has earned and maintained a 4.0

GPA, while also raising her three year old son, Aiden. She is inspired by nature and the

biology of various organisms, and she hopes to translate her observations and

conclusions in ways that can help people.

Chynna Spangle is a student at Austin Community College (ACC) in Austin, Texas.

She is a Geology Major and works as a Lab/Administrative Assistant for the Geology

Department at ACC. She also dedicates many hours as the Vice-President of ACC’s

Geology Club. Chynna is passionate about getting girls involved in STEM fields, and is

an S-STEM scholarship recipient. Chynna plans to transfer to the Jackson School of

Geosciences at UT Austin in Spring 2015. She will likely go into Sedimentology or

Petroleum Geology. Chynna is also working on a GIS (Geographic Information

Systems) certificate. She is dedicated to her two year old son. When not involved with

parenting and academia, Chynna spends her free time drawing stratigraphic columns and

hunting for fossils. She is also a devout fencer.

Andrea Gonzalez is an undergraduate student at Austin Community College in

Austin, Texas. Her major Engineering and her expected graduation date is spring 2016.

She plans to transfer to the University of Texas at Austin to pursue a bachelor’s degree

in Chemical Engineering or Environmental Engineering. Andrea was awarded the S-

STEM scholarship in January 2015 and works on the biotech team. She anticipates

induction into Phi Theta Kappa Honors Society this semester. Andrea enjoys nature

and works to protect our natural resources.

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Elizabeth Savercool is a third year, full time student attending Austin Community

College (ACC) in Austin, TX. She will be graduating from ACC in the spring of 2016

with an Associate's degree in Physics. She will transfer to a four year university to pursue

a PhD in Physics. Elizabeth was awarded the S-Stem scholarship and is currently working

with her fellow scholarship recipients on a sustainability project. She is on the Design

Optimization/Fabrication team. She is an officer in ACC's Society of Physics Students

organization, and a member of ACC's American Chemical Society. She currently works as

a registered nurse for a pediatric home health agency and is raising a teenage daughter.

She is inspired by the dynamic field of Physics and its infinite possibilities.

Kelli Kathleen Boydston is a student at Austin Community College located in

Austin, Texas. She is majoring in Geology and is graduating with an A.A.S. in Spring

2016. Kelli also plans on furthering her education at the University of Texas at Austin,

also majoring in Geology. She is involved in the Public Communications/ Technical

Writing area of the S-STEM scholarship project. Kelli has enjoyed spending time

volunteering throughout her years at Austin Community College while being a member of

Phi Theta Kappa. She is happiest being with her two children, reading, studying, and

being outdoors.

Mariah Farrar is from Dallas, Texas and is currently a sophomore at Austin

Community College in Austin, Texas. Mariah is majoring in Biotechnology and will be

transferring to the University of Texas at Austin - College of Natural Sciences in fall

2015. She is slated to graduate from the University of Texas in 2017. Mariah is a part of

the Biotech team in the S-STEM program and is also a member of the Phi Theta Kappa

Honor Society.

Michael Ryan Hixson is an engineering scholar at Austin Community College. He

will be graduating with an Associate of Science in Engineering (General) from ACC

during May of 2015. He is currently aspiring to graduate from ACC and transfer to the

University of Texas in continuation of his engineering education. Michael is part of the

Honors program as well as the local chapter of the honor society Alpha Gamma Pi. In

addition to his engineering curriculum, Michael provides supplemental instruction for

elementary algebra at ACC Cypress Creek. He also volunteers his problem solving skills

at Bridges to Growth in Georgetown, TX. Michael is Part of ACC’s Design and

Fabrication team that works to produce pro-environment solutions. He was born in

Austin, Texas and has lived and studied in many different parts of the U.S. including;

Los Angeles CA, Asheville NC, Glendale CA, and Las Vegas NV. He is happy to be

back home in Austin with the blessing of time, and the support of many people.

Page 40: Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed Residential Greywater

Michelle Lynne McGill is a student at Austin Community College in Austin,

Texas. Michelle hopes to graduate by the end of 2016 with an A.S. in Biotechnology.

Outside of class, Michelle enjoys reading, going to concerts/comedy shows and

spending time with her family.

Miranda Peterson is a full-time student at Austin Community College. She is

working toward an A.S. in Environmental Science and is slated to graduate in the fall of

2015. Miranda joined S-STEM at the end of 2014. Miranda has played a role in the

public communications and technical writing team for the P3 project regarding the solar

disinfection of residential greywater. Miranda has recently joined the ACC Geology

Club and is enjoying her school club. Outside of school, she enjoys hiking and the

outdoors. She looks forward to combining this passion and education towards a career

as a Park Ranger.

Thomas Thompson is a student at Austin Community College located in Austin,

Texas. Thomas is majoring in Environmental Science and is scheduled to graduate in

2015 with an A.A.S in Environmental Science/ Technology. He is an S-Stem

scholarship recipient working with the Public Communications and Technical Writing

team. Thomas has done volunteer work for the National Forest Service in Summit

County, Colorado and also enjoys volunteering with the Sustainable Food Center in

Austin, Texas.

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Dylan J Reynolds is a student at Austin Community College located in Austin, Texas. Dylan is majoring in

Electrical Engineering and Computer Science and is expected to graduate in 2016 with an A.S. in Engineering.

He is a member of the Phi Theta Kappa Honor Society and a member of the S-STEM research group. Dylan

was awarded the S-STEM scholarship in 2015 and is participating in the Evaluation and Expansion of Solar

Disinfection Method of Residential Greywater E.P.A. Project P3 - Grant: #SU 835926. He is part of the

Biotech Team which is a subgroup of the S-STEM project. His role contributes in data collection and analysis

which involves running experiments as well as analyzing and interpreting collected data.

Katy Jo Leatham is a sophomore student at Austin Community College located in Austin, Texas. Katy is

majoring in Civil Engineering and is projected to graduate in 2017 with a B.S. in Civil Engineering. Katy’s

area of concentration in the S-STEM group is data collection, analysis and graphing. Katy is a Phi Theta Kappa

member and also a Texas Real Estate agent with an interest in structural development.

Other Participating Students:

Emma Drueke

Christina Edgar

Samer Hasan

Stephanie Gage

Isabelle Jaimes

Joe Rodriguez

Michelle Lebeouf

Paul Newcomb

Matthew Schulze

Marcus Searle

Gretchen Smith

Josh Walden

Page 42: Final Project Report - The Evaluation and Expansion of the Solar Disinfection Method of Reclaimed Residential Greywater

Current and Pending Support

Confidentiality

By submitting an application in response to this solicitation Austin Community College District

grants EPA permission to make limited disclosures of the application to technical reviewers both

within and outside the Agency for the express purpose of assisting the Agency with evaluating

the application. Information from a pending or unsuccessful application will be kept confidential

to the fullest extent allowed under law; information from a successful application may be

publicly disclosed to the extent permitted by law.