Filtration - Phenomenexphx.phenomenex.com/lib/br65751208_i.pdf · • Rapid filtration of buffers,...

Mobile Phase Filtration ....................................................................................................................

Sample Preparation ...........................................................................................................................

Column Protection ..............................................................................................................................

Filtration Solutions for Chromatography

The Difference is Filtration

2

Proper sample and mobile phase filtration are essential to preserving the integrity of your sample and system components. Without it, irreparable and costly damage to your expensive system and column can occur and the results of your chromatography can be compromised.

Phenomenex filtration solutions help protect your valuable samples and system components through every step of your analysis.

© 2010 Phenomenex, Inc. All rights reserved.

The Difference is Filtration

FilTereD Samples Can Help You . . .

Increase Column Lifetime•

Improve Your Analytical Results•

Decrease System Downtime and Costly Repairs•

NoN-FilTereD Samples Can Cause . . .

Baseline Noise, Ghost, and Split Peaks•

High Operating Pressures•

Damage to Detectors, Pumps, and Valves•

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2

4

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5 67

8

9

10

11

0 5 6 min

40 5 6 7 min

1

2

34

56

7

8

9

10

11

The Difference is FiltrationThe Difference is Filtration

3

Table of C

ontents

................pp. 4-9

......................p. 6

......................p. 7

......................p. 8

......................p. 8

......................p. 8

......................p. 9

............pp. 10-17

............ pp. 12-13

....................p. 14

....................p. 14

............ pp. 15-17

............pp. 18-22

....................p. 22

............ pp. 20-22

SampleInjector

HPLCPump

MobilePhase

HPLCDetector

HPLC ColumnColumn

Protection

SolventWaste

SampleInjector

HPLCPump

MobilePhase

HPLCDetector

HPLC ColumnColumn

Protection

SolventWaste

SampleInjector

HPLCPump

MobilePhase

HPLCDetector

HPLC ColumnColumn

Protection

SolventWaste

Mobile Phase Filtration

Phenex™ Filter Membranes

FilterSys™ Mobile Phase Vacuum Filtration System

HPLC Mobile Phase Reservoir

Bottle Filter Caps

Replacement Valve Caps

Inlet Filters

Sample Preparation

Syringe Filters

Centrifugal Filters

All-Plastic Disposable Syringes

Solid Phase Extraction

SampleInjector

HPLCPump

MobilePhase

HPLCDetector

HPLC ColumnColumn

Protection

SolventWaste

SampleInjector

HPLCPump

MobilePhase

HPLCDetector

HPLC ColumnColumn

Protection

SolventWaste

Column Protection

In-Line Filters

SecurityGuard™


4

Why is mobile phase filtration important? Unfiltered mobile phase may contain particulates and bacterial

contaminants that can build up and create a chain reaction of

system and column problems. Check valves, pump seals and

the injector may be damaged by particulates passing through

the system. Once these particulates reach your column, the

buildup will result in pressure increases, causing your system

to work harder, or even shut down. Trace amounts of contami-

nants can also produce split peaks or extraneous unidentifi-

able peaks, leading to unacceptable results.

How does mobile phase filtration work?There are two solutions available to transfer solvents for filtration.

• Apickupadaptercanbeusedtodirectlyfeedthesol-

vent from the source container into the filtration system

• Afunnelcanbeusedtoholdandtransferthesolventfor

filtration

With either solution, solvents are passed through a 47 mm di-

ameter Phenex™ filter membrane which is placed between the

FilterSys™ mobile filtration system’s fritted glass support base

and the sample reservoir. The filtered, particulate-free solvents

are then stored in plasticizer-free glass reservoirs for delivery

to the chromatographic system. When the filtered solutions are

ready for use, solvent inlet filters remove any buffer that may

have precipitated or any bacterial growth that has occurred

before they enter the system.

Together, this system allows for the rapid filtration of particu-

late matter along with the removal of undissolved buffers from

your solvents.

Protect your chromatographic equipment from mobile phase elements that can damage components and produce poor results.


Mobile Phase FiltrationMobile Phase Filtration

5

Mob

ile Phase Filtration

Phenex™ Filter Membranesp. 6

FilterSys™ Mobile Phase Vacuum Filtration Systemp. 7

HPLC Mobile Phase Reservoirp. 8

to HPLC Pump

toVacuumLine

Valve Capsp. 8

Bottle Filter Capsp. 8

Solvent Pickup Adapterp. 7

Inlet Filterp. 9

Unfiltered Solvent Filtered Solvent

Flow

6

Mobile Phase FiltrationPhenex™ Filter Membranes

Above filter membranes are non-sterile.*MCE = mixed cellulose estersFor information on Filtration System, please see page 7.

remove particulates from solvents

Phenex™ Filter Membranes reduce sample preparation times and improve chromatography in one step by filtering solvents.

Wide selection of membrane sizes and types •

Virtually free of unwanted plasticizers or contaminants•

Excellent chemical resistance to almost all laboratory solvents•

Choose the proper membrane type for your analysis:

Regenerated Cellulose (RC) is recommended for general filtration, de-gassing •solvents, and HPLC mobile phases (both aqueous and organic)

Nylon 66• ® (NY) is hydrophilic and makes the filtering of aqueous solvents simple, without pre-wetting

Polytetrafluoroethylene (PTFE, Teflon• ®) is hydrophobic and is recommended for the filtration of non-aqueous solutions

Polyethersulfone (PES) has excellent flow speeds with a wide pH range (2-12) •and high filterable volume used for aggressive solvents

Cellulose Acetate (CA) is recommended for buffers and other aqueous •solutions

Cellulose Nitrate Ester (MCE)* is recommended for •particle collection, sample pre-treatment, and chemotaxis

email: [email protected] I web: www.phenomenex.com

Ordering InformationPhenex Filter Membranes

Part No.

Pore Size (μm)

DiscDiameter

(mm)Membrane

Material Unit PriceRegenerated Cellulose (RC) AF0-8440 0.45 13 RC 100/pkAF0-8441 0.2 13 RC 100/pkAF0-8442 0.2 25 RC 100/pkAF0-8443 0.45 47 RC 100/pkAF0-8444 0.2 47 RC 100/pkPTFE (Teflon®)AF0-0509 0.2 13 PTFE 100/pkAF0-0511 0.2 25 PTFE 100/pkAF0-0512 0.45 25 PTFE 100/pkAF0-0513 0.2 47 PTFE 100/pkAF0-0514 0.45 47 PTFE 100/pkCellulose Acetate (CA) AF0-8434 0.45 13 CA 100/pkAF0-8435 0.2 13 CA 100/pkAF0-8436 0.45 25 CA 100/pkAF0-8437 0.2 25 CA 100/pkAF0-8438 0.45 47 CA 100/pkAF0-8439 0.2 47 CA 100/pk

Phenex Filter Membranes

Part No.

Pore Size (μm)

DiscDiameter

(mm)Membrane

Material Unit PriceNylon (NY)AF0-0500 0.45 13 Nylon 100/pkAF0-0501 0.2 25 Nylon 100/pkAF0-0502 0.45 25 Nylon 100/pkAF0-0503 0.2 47 Nylon 100/pkAF0-0504 0.45 47 Nylon 100/pkAF0-0505 0.2 90 Nylon 5/pkAF0-0506 0.45 90 Nylon 5/pkPolyethersulfone (PES)AF0-8445 0.2 25 PES 100/pkAF0-8446 0.45 25 PES 100/pkAF0-8447 0.2 47 PES 100/pkAF0-8448 0.45 47 PES 100/pkCellulose Nitrate Ester (MCE)*AF0-8449 0.80 13 MCE 100/pkAF0-8450 0.45 13 MCE 100/pkAF0-8451 0.80 25 MCE 100/pkAF0-8452 0.45 25 MCE 100/pkAF0-8453 0.80 47 MCE 100/pkAF0-8454 0.45 47 MCE 100/pk

7


Use filter membranes with this rapid filtration system!

Prevents pump and system component damage•

Rapid filtration of buffers, organics and corrosive liquids•

Removes damaging microparticulates and bacterial contaminants•

Use filter membranes with this rapid filtration system!

This glass adapter enables direct pickup of mobile phase solvent using the Phenomenex FilterSys™. It replaces the tedious and dangerous pour-and-wait funnel filtration method by drawing solvent directly from the reagent bottle, which is the safest way to transfer and filter solvents.

4 mm PTFE stopcock with ¼ in. OD outlet•

PTFE connector with compression fittings for ¼ in. OD tubing•

3 feet of ¼ in. OD PTFE tubing•

FilterSys™ Mobile Phase Vacuum Filtration System

Solvent Pickup Adapter

WARNING: The apparatus should be used with a water aspiration line, not a true vacuum line, unless secured behind an appropriate safety shield.


Ordering InformationSolvent Pickup AdapterPart No. Description Unit PriceAH0-2947 Mobile Phase Pickup Adapter, 47 mm ea

Ordering InformationMobile Phase Filtration SystemPart No. Description Unit PriceComplete AssemblyAH0-1566 FilterSys, 47 mm, 300 mL funnel with 1 L vacuum flask eaAH0-3314 FilterSys, 47 mm, 500 mL funnel with 2 L vacuum flask eaAH0-3315 FilterSys, 47 mm, 1000 mL funnel with 4 L vacuum flask eaComponent PartsAH0-1567 Fritted support base, 47 mm, 40/35 taper eaAH0-1568 Funnel, graduated, 300 mL, 47 mm ea AH0-3323 Funnel, graduated, 500 mL, 47 mm ea AH0-3324 Funnel, graduated, 1000 mL, 47 mm ea AH0-1569 1 liter filter flask, 40/35 taper eaAH0-3321 2 liter filter flask, 40/35 taper eaAH0-3322 4 liter filter flask, 40/35 taper eaAH0-1570 Aluminum clamp, 47 mm ea

8

Mobile Phase FiltrationHPlC Mobile Phase reservoir

Low-leaching (low alkali), borosilicate glass•

Chemically inert (no plasticizers), internal PTFE seal•

replacement Valve Cap

For Gl45 Neck Mobile Phase reservoirs

Valve caps provide an inert PTFE (Teflon®) seal against the solvents inside. An all-Teflon block design with stainless steel heli-coils grips the Teflon tubing.

Supplied with • 1/8 in. fitted tubes, will not twist when tightened

Pressure rated to 15 psi (1 bar) and autoclavable•

Bottle Filter Caps

reduce solvent evaporation and gas absorption

Bottle filter caps ensure constant pH levels and increase chromatography reproducibility by minimizing the reabsorption of dissolved gases back into the solvent

All cap parts are compatible with most common HPLC solvents•

Neatly seals to prevent particulates from entering the solvent bottle•


Ordering InformationFilter CapPart No. Description Cap Size Unit PriceAH0-1565 Filter Reservoir Cap 38 mm ea

Ordering InformationReplacement Valve Cap for GL45 Threaded BottlesPart No. Description Unit PriceAH0-4141 3-Way Valve Cap, GL45 threads, solid PTFE (Teflon®) block

constructionea

Ordering InformationReservoir and Valve Cap AssemblyPart No. Description Unit PriceAH0-4142 HPLC Reservoir, 1000 mL clear glass, GL45 wide-mouth, includes

3-way Valve Capea

AH0-4143 HPLC Reservoir, 2000 mL clear glass, GL45 wide-mouth, includes 3-way Valve Cap

ea

9

Mobile Phase Filtrationinlet Filters

Protect HPlC pump heads and check valves

The Solvent Saver™ Inlet Filters minimize costly system repairs and reduce system downtime by cleaning solvents before analysis. Phenomenex offers two types of solvent inlet filters to fit your analysis: Metal-Free/Biocompatible and Stainless Steel.

Metal-Free / Biocompatible

This is a great choice for sensitive biochromatography and ion chromatography applications where metal surfaces may corrode or interact with samples.

Flat bottom design uses all available mobile phase•

High surface area for long filter life•

Stainless Steel

The best option for general applications that use non-corrosive chemicals.

Protects pumps and check valves•

Easy to replace•

Low cost•

Universal fit adapter(sold separately AQ0-8339)

slip-fits onto 1.5,2.2 or 3.0 mm IDtubing

1/4 in.-28 flangelessfitting (included) attachesto 1/8 in. OD tubing

PEEK® Filter


Ordering InformationSolvent Saver Inlet Filter - Metal FreePart No. Description Unit PriceAH0-1562 Solvent Saver Inlet Filter with 10 µm PEEK

filter with Flangeless fitting for 1/8 in. OD tubingea

AQ0-8339 Solvent Saver Unifit Adapter, Tri-Step Tubing Connector, PEEK eaAQ0-2949 Flangeless Nut and Ferrule for 1/16 in. OD tubing,

¼ in.-28 threads, red Delrin10/pk

AQ0-2950 Flangeless Nut and Ferrule for 1/8 in. OD tubing,¼ in.-28 threads, green Delrin

10/pk

AT0-2953 Teflon Tubing, 5 ft. L x 1/16 in. OD x 1/32 in. (0.031 in.) ID eaAT0-2955 Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID ea

Ordering InformationSolvent Inlet Filters - Stainless SteelPart No. Description Unit PriceAF0-0356 Solvent Inlet Filter, 2 µm, for 1/16 in. ID tubing eaAF0-0359 Solvent Inlet Filter, 2 µm, for 1/8 in. ID tubing eaAT0-2953 Teflon Tubing, 5 ft. L x 1/16 in. OD x 1/32 in. (0.031 in.) ID eaAT0-2955 Teflon Tubing, 5 ft. L x 1/8 in. OD x 1/16 in. (0.062 in.) ID ea

Sample Preparation

10

Why is sample preparation important?Sample preparation products protect your column and sys-

tem from sample particulates and endogenous contaminants

which lead to shorter column lifetime, increased system down-

time and unacceptable results.

• Filteringyoursamplesreducestheriskofparticulatecon-

tamination to your column and your system components.

•Cleansamplesextendcolumnlifetimesanddecrease

the incidence of high pressures caused by particulates

and contaminant build up at the head of the column.

•Effectivefiltrationalsosavesyoursystem’srotorseals,

valve stators, and other moving components from un-

necessary wear and damage that can result from undis-

solved sample particulates grinding away at the system

components.

•SPEphasesofferspecificselectivityfortargetanalytes

allowing for an aggressive sample wash to remove en-

dogenous contaminants.

How does sample preparation work?

• Phenexsyringefiltersutilizeafiltermembranetoprevent

particulates from entering the injector and the HPLC

column. This in turn protects your system from over-

pressurization and prevents split peaks in your chroma-

tography. Unfiltered samples are easily pushed through

the filter housing with a syringe and then collected for

analysis.

•Phenexcentrifugalfiltersutilizecentrifugalforcetodrive

the samples through the filter quickly without effort on

the part of the chemist.

•SPEutilizesamulti-stepchromatographicprocessto

separate mixtures into desired and undesired compo-

nents. Analytes of interest are either retained by the

stationary phase for collection at a later step or they are

collected for analysis or further sample preparation.

eliminate contaminants and undesirable components before your samples reach your column or system!

Sample Preparation

Sample PreparationSample Preparation

11

Sam

ple P

reparation

Solid Phase Extractionpp. 15-17

Contaminated Samples

Clean SampleReady for Analysis

Centrifugal Filtersp. 14

Syringe Filterspp. 12-13

All-Plastic Disposable Syringesp. 14

12

Sample PreparationPhenex™ Syringe Filters

Sample Description recommended Filter Pore Size

General aqueous or mixed organic samples prior to HPLC analysis with column packed with > 3 µm particles. General clarification of GC, SFC, CE, and GPC samples. 0.45 µm

Viscous samples or samples containing high levels of particulate matter.

General aqueous or mixed organic samples prior to HPLC analysis with columns packed with ≤ 3 µm particles. Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples. 0.20 µm

Gas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods.

Viscous samples such as serum, plasma, or other biological matrices. Solutions with high particulate load (e.g., some environmental or food and beverage applications). Glass Fiber filter with 0.45 µm filter

2. Select pore size based on the nature of your sample and chromatographic method

Aqueous(Particulate-laden samples may require pre-filtration)

Solvent Mixtures Tissue Culture Media, Buffers

Protein Analysis / Biological Samples

RC(Regenerated Cellulose)

CA(Cellulose Acetate)

PES(Polyethersulfone)

SolventsNon-Aqueous Aqueous Mixtures

Hydrophobic Hydrophilic

PTFE(Polytetrafluoroethylene)

RC(Regenerated Cellulose)

3. Select filter membrane according to the characteristics of your sample and filtering objective

1. Select filter diameter based on sample volume

if your sample volume is:

≤ 2 ml Sample Volume 2 to 10 ml Sample Volume 10 to 100 ml Sample Volume

4 mm Diameter 15 - 17 mm Diameter 25 - 28 mm Diameter

Protect injector valves, prevent costly rework, and reduce system downtimes

Phenex™ syringe filters increase column lifetime and improve chromatographic reproducibility. Phenex offers a variety of chemically compatible syringe filter membranes that are ideal for any application. Proper membrane and size selection are the keys to choosing the best product to maintain sample integrity while protecting your HPLC or GC column system from particulate contamination.

Select your filter in three eASY steps:


13

Sample PreparationNon-Sterile Syringe Filters

Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP). Luer lock inlet/slip outlet connections unless otherwise indicated.1. 17 mm diameter. 2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles > 1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite®. 4. Cellulose acetate is surfactant-free. 5. 26 mm diameter. 6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA. 7. 28 mm diameter. 8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance. 9. Larger quantity purchases at significant savings are available.

Sterile Syringe Filters

Sterile syringe filters from Phenomenex are ready-to-use, individually blister packaged units, offering high flow rates at low inlet pressures, offering rapid sterile filtration.


Ordering Information

Phenex Syringe Filters 4 mm Diameterfor ≤ 2 mL sample volumes

15 - 17 mm Diameterfor 2 – 10 mL sample volumes

25 - 28 mm Diameterfor 10 – 100 mL sample volumes

Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price

0.45 µm

Phenex-RC(Regenerated Cellulose)

AF0-3103-12 100/pk AF0-2103-12 100/pk AF0-8103-12 5 100/pk

AF0-3103-52 500/pk AF0-2103-52 500/pk AF0-8103-52 5 500/pk

Phenex-PES 3

(Polyethersulfone)

— — AF2-5108-12 1 100/pk AF0-8108-12 7 100/pk

— — — — AF0-8108-52 7 500/pk

Phenex-PTFE 6

(Polytetrafluoroethylene)

AF0-3102-12 100/pk AF0-2102-12 100/pk AF0-1102-12 100/pk

AF0-3102-52 500/pk AF0-2102-52 500/pk AF0-1102-52 500/pk

Phenex-NY(Nylon)

AF3-3107-12 100/pk AF0-2107-12 100/pk AF0-1107-12 100/pk

AF3-3107-52 500/pk AF0-2107-52 500/pk AF0-1107-52 500/pk

Phenex-GF/CA 2,3,4

(Glass Fiber/Cellulose Acetate)

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock.

AF0-8B09-12 7 100/pk

AF0-8B09-52 7 500/pk

0.20 µm

Phenex-RC(Regenerated Cellulose)

AF0-3203-12 100/pk AF0-2203-12 100/pk AF0-8203-12 5 100/pk

AF0-3203-52 500/pk AF0-2203-52 500/pk AF0-8203-52 5 500/pkPhenex-PES 3

(Polyethersulfone)— — — — AF0-8208-12 7 100/pk

— — — — AF0-8208-52 7 500/pk

Phenex-PTFE 6

(Polytetrafluoroethylene)

AF0-3202-12 100/pk AF0-2202-12 100/pk AF0-1202-12 100/pk

AF0-3202-52 500/pk AF0-2202-52 500/pk AF0-1202-52 500/pk

Phenex-NY(Nylon)

AF3-3207-12 100/pk AF0-2207-12 100/pk AF0-1207-12 100/pk

AF3-3207-52 500/pk AF0-2207-52 500/pk AF0-1207-52 500/pk

Phenex-CA 4

(Cellulose Acetate)

— — — — — — AF0-8204-12 7 100/pk

— — — — — — AF0-8204-52 7 500/pk

Phenex-GF/CA 2,3,4

(Glass Fiber/Cellulose Acetate)

An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. Excellent for filtration of tissue culture media, general biological sample filtration and clarification. Outlet connection is luer lock.

AF0-8A09-12 7 100/pk

AF0-8A09-52 7 500/pk

1.20 µmPhenex-GF 2,3

(Glass Fiber)Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. Outlet connection is luer lock.

AF0-8515-12 7 100/pk

AF0-8515-52 7 500/pk

Ordering Information Sterile Syringe Filters

Part No.Pore

Size (μm)Disc

Diameter (mm)Membrane

Material Unit PriceAF0-8455 0.2 28 CA Luer/Slip 50/pkAF0-8456 0.45 28 CA Luer/Slip 50/pkAF0-8457 0.2 28 PES Luer/Slip 50/pkAF0-8458 0.45 28 PES Luer/Slip 50/pkAF0-8459 0.2 25 RC Luer/Slip 50/pkAF0-8460 0.45 25 RC Luer/Slip 50/pkAF0-8461 0.2 25 PTFE Luer/Slip 50/pk

14

Sample Preparation

Convenient filtration of multiple HPLC and GC samples•

High recovery for small samples•

Nylon, PTFE (Teflon• ®), and Cellulose Acetate membrane materials

Phenex™ disposable centrifugal filter units provide a convenient means of filtering multiple HPLC and GC samples without the necessity of manually forcing each sample through the filter with a syringe. All you need is a laboratory centrifuge with sufficient capacity for the number of samples to be filtered at one time. Centrifugal force drives the sample through the filter quickly without effort on the part of the chemist.

Samples contact only the Phenex filter unit itself and the polypropylene receiver tube. The upper portion of the tube containing the membrane filter can be discarded. The receiver tube serves as a container for the filtered sample and can be retained as long as desired. Separate caps are supplied for the sample chamber and the receiver tube.

Filter membrane materials available are Nylon 66, PTFE (Teflon®) and Cellulose Acetate (CA). Nylon 66 Phenex filter units are recommended for most HPLC and GC sample filtration work. They are chemically resistant, compatible with all commonly used solvents, and virtually free of extravctable materials that could contaminate samples. PTFE (Teflon®) Phenex filters are designed to handle more aggressive organic solvents, whereas Cellulose Acetate (CA) is especially well-suited for the filtration of proteinaceous samples in aqueous solvents.

Use for all syringe filter applications•

Luer-lock outlet makes connection easy•

Capacities ranging from 3 to 20 mL•

Made of ultra-clean, high-purity plastic•

Phenex™ Disposable Centrifugal Filter

All-Plastic Disposable Syringes

Above centrifugal filter units are non-sterile


Ordering InformationAll-Plastic Disposable SyringesPart No. Description Capacity (mL) Unit PriceAS0-8408 Plastic Disposable Syringes, Luer-lock 3 100/pkAS0-8409 Plastic Disposable Syringes, Luer-lock 5 100/pkAS0-8410 Plastic Disposable Syringes, Luer-lock 10 100/pkAS0-8411 Plastic Disposable Syringes, Luer-lock 20 100/pk

Ordering InformationCentrifugal Filter Units

Part No. Pore Size (μm)

Volumes (mL)Sample/Receiver

MembraneNon-Sterile Unit Price

AF0-0438 0.2 2.0 / 5.0 Nylon 25/pkAF0-0439 0.45 2.0 / 5.0 Nylon 25/pkAF0-0440 0.2 2.0 / 5.0 PTFE 25/pkAF0-0441 0.45 2.0 / 5.0 PTFE 25/pkAF0-8353 0.2 2.0 / 5.0 Cellulose Acetate 25/pkAF0-8354 0.45 2.0 / 5.0 Cellulose Acetate 25/pk

15

Sample Preparation

Quality Solid Phase extraction (SPe) products for your sample pre-treatment needs

Sample Cleanup•

Sample Concentration•

Desalting•

Solvent Switching•

Solid Phase extraction

Choose Your ideal Sample Pre-treatment Solution

SPe Mechanism Analyte Functional Group Sorbent Functional Group Strata Sorbent

reversed Phase

Rhydrocarbon

aromatic

hydrocarbon

aromatic

C18-E, C18-U, C8, C18-T, X, XL

X, Phenyl, SDB-L, XL

Normal Phase

R - OH

hydroxyl

R - NH2

amino

CN

polar

OH

polar

CN, NH2

Si-1, CN

ion-exchange

NR4+ strong base

RNH3+ weak base

RSO3- strong acid

RCO2- weak acid

-O2C—weak cation -O3S—strong cation

+H3N—weak anion

+R3N—strong anion

X-CW, WCX

X-C, SCX, XL-C

X-AW, NH2

SAX, X-AW

+analyte

-analyte

Impurities

Target analyte

Impurities

CONDITION LOAD WASH ELUTE


16

Sample Preparation

Traditional Silica-Based Sorbents

Achieve reproducible results•

Wide variety of phases available for optimal selectivity•

Several formats available for low and high-throughput•

Inert sorbent•

Strata® SPe


Normal Phase Sorbents - Cyano (CN)Format

Sorbent Mass Part Number Unit Price

Tube100 mg 8B-S007-EAK 1 mL (100/box) 200 mg 8B-S007-FBJ 3 mL (50/box) 1000 mg 8B-S007-JCH 6 mL (30/box)

96-Well Plate25 mg 8E-S007-CGB 2 Plates/Box50 mg 8E-S007-DGB 2 Plates/Box

100 mg 8E-S007-EGB 2 Plates/Box

Normal Phase Sorbents - Silica (Si-1)

FormatSorbent

Mass Part Number Unit PriceTube

100 mg 8B-S012-EAK 1 mL (100/box) 200 mg 8B-S012-FBJ 3 mL (50/box)

1 g 8B-S012-JCH 6 mL (30/box)96-Well Plate

25 mg 8E-S012-CGB 2 Plates/Box50 mg 8E-S012-DGB 2 Plates/Box


Normal Phase Sorbents - NH2/WAX

FormatSorbent

Mass Part Number Unit PriceTube 100 mg 8B-S009-EAK 1 mL (100/box)

200 mg 8B-S009-FBJ 3 mL (50/box) 1 g 8B-S009-JCH 6 mL (30/box)

96-Well Plate 25 mg 8E-S009-CGB 2 Plates/Box

50 mg 8E-S009-DGB 2 Plates/Box100 mg 8E-S009-EGB 2 Plates/Box

ion-exchange Sorbents - SCX (strong cation exchange)

FormatSorbent



96-Well Plate 25 mg 8E-S010-CGB 2 Plates/Box 50 mg 8E-S010-DGB 2 Plates/Box


Mixed-Mode Sorbents Screen-A

FormatSorbent


100 mg 8B-S019-EAK 1 mL (100/box) 200 mg 8B-S019-FCH 6 mL (30/box) 500 mg 8B-S019-HCH 6 mL (30/box)


ion-exchange Sorbents - SAX (strong anion exchange)

FormatSorbent





reversed Phase Sorbents - C18-e (endcapped)Format

Sorbent Mass Part Number Unit Price

Tube100 mg 8B-S001-EAK 1 mL (100/box) 200 mg 8B-S001-FBJ 3 mL (50/box) 1000 mg 8B-S001-JCH 6 mL (30/box)

96-Well Plate25 mg 8E-S001-CGB 2 Plates/Box50 mg 8E-S001-DGB 2 Plates/Box


ion-exchange Sorbents - WCX (weak cation exchange)

FormatSorbent


100 mg 8B-S027-EAK 1 mL (100/box) 200 mg 8B-S027-FBJ 3 mL (50/box)

1 g 8B-S027-JCH 6 mL (30/box)96-Well Plate 25 mg 8E-S027-CGB 2 Plates/Box


17

Sample Preparation

SPe Vacuum Manifolds 96-Well Plate Manifold

Functionalized Polymeric Sorbents

Higher loadability than silica-based sorbents•

Less solvent required when compared to silica-based sorbents•

Decreased blow-down time attributed to lower solvent requirements•

pH stable from 1-14•

Strata™-X SPe

For additional sizes and formats, please visit www.phenomenex.com/spe


Part No. Description Unit Price24 - Position Vacuum ManifoldAH0-6024 SPE 24-Position Vacuum Manifold Set, complete assembly ea12 - Position Vacuum ManifoldAH0-6023 SPE 12-Position Vacuum Manifold Set, complete assembly ea10 - Position Vacuum ManifoldAH0-7502 SPE 10-Position Tall-Boy Vacuum Manifold, complete assembly ea

Part No. Description Unit PriceAH0-7284 96-Well Plate Manifold, Acrylic ea

Strata-X-C

FormatSorbent

Mass Part Number Unit PriceTube 30 mg 8B-S029-TAK 1 mL (100/box)

60 mg 8B-S029-UBJ 3 mL (50/box) 500 mg 8B-S029-HCH 6 mL (30/box)

96-Well Plate 10 mg 8E-S029-AGB 2 Plates/Box

30 mg 8E-S029-TGB 2 Plates/Box60 mg 8E-S029-UGB 2 Plates/Box

Strata-X

FormatSorbent


30 mg 8B-S100-TAK 1 mL (100/box) 60 mg 8B-S100-UBJ 3 mL (50/box)

500 mg 8B-S100-HCH 6 mL (30/box)96-Well Plate

10 mg 8E-S100-AGB 2 Plates/Box30 mg 8E-S100-TGB 2 Plates/Box60 mg 8E-S100-UGB 2 Plates/Box

Strata-X-CW

FormatSorbent





Strata-X-AW

FormatSorbent





Strata-Xl

FormatSorbent


30 mg 8B-S043-TAK 1 mL (100/box) 60 mg 8B-S043-UBJ 3 mL (50/box) 500 mg 8B-S043-HCH 6 mL (30/box)

96-Well Plate30 mg 8E-S043-TGB 2 Plates/Box200 mg 8E-S043-FGB 2 Plates/Box400 mg 8E-S043-5GB 2 Plates/Box

Strata-Xl-C

FormatSorbent


30 mg 8B-S044-TAK 1 mL (100/box) 60 mg 8B-S044-UBJ 3 mL (50/box) 500 mg 8B-S044-HCH 6 mL (30/box)

96-Well Plate30 mg 8E-S044-TGB 2 Plates/Box

Column Protection

18

Why is column protection important?Column protection systems reduce the amount of sample

and mobile phase contaminants from building up at the head

of the column, which can otherwise degrade chromatography,

increase system backpressure, and shorten column lifetimes.

Protection systems also safeguard HPLC columns and detec-

tors from damaging pump and rotor seal particulates.

How does column protection work?Column protection systems protect your HPLC columns and

detectors from microparticulates in the sample, solvent, or

wear on the polymeric seals of the pump or injector. These

protection products trap particulates before they can reach the

column or detector. Contaminants can clog frits, irreversibly

bind to column packings, degrade analytical performance, and

even damage expensive detector flow cells.

There are two types:

•TheSecurityGuard™ system protects the HPLC column

and detector by trapping both particulates and chemical

contaminants with inexpensive disposable cartridges.

Short cartridge lengths protect your HPLC columns

without altering chromatography.

• In-linefiltersremovefineparticulatesfromthesolvent

stream. They also remove microparticulates as small as

0.5 µm before they clog your column and degrade your

separation.

Prevent damaging micro-particulates, system particles, and bacterial contaminants from entering your column and detector.

Column Protection

Column ProtectionColumn Protection

19

Colum

n Protection

Analytical

SemiPrep and Preparative

Guard Cartridge System SecurityGuardpp. 20-21

Guard Cartridge System SecurityGuard PREPpp. 20-21

In-Line Filtersp. 22

In-Line Filtersp. 22

20

Column ProtectionSecurityGuard™

it’s protection without any change

SecurityGuard can protect valuable HPLC columns from the damaging effects of chemical contaminants and microparticulates without altering chromatographic results. This protection can lead to an extension of column life. Simply put, every chromatographer must have one.

Trap Contaminants

The SecurityGuard disposible cartridges trap unwanted contami-nants from entering and damaging your column, thereby extending column lifetime.

From Injector G

uard C

artridge S

ystem H

PLC

Colum

n

> >

> >

> >

> >

> >

> >

> >

>

ContaminantsTrapped

Contaminants

Compoundsof Interest

To Detector

Protect your column from particulate matter and chemical attacks with SecurityGuard Cartridge System

Extends your HPLC column lifetime•

Will not alter your chromatography•

Compatible with virtually all HPLC columns•

Easy to determine when to change cartridge•

Easy to use•

SecurityGuard is a universal guard cartridge system that acts as both a chemical and a mechanical filter, which extends HPLC column lifetime. Each SecurityGuard phase is matched to the column stationary phase.


21

Column Protection

Ordering Information

Cartridges and Holders

Use Cartridges (mm): 4.0 x 2.0 4.0 x 3.0 10 x 10.0 15 x 21.2 15 x 30.0

If your HPLC column Internal Diameter, ID (mm) is:

Analytical SemiPrep PREP 2.0 - 3.0 3.2 - 8.0 9.0 - 16.0 18.0 - 29.0 30.0 - 49.0

*For use with saccharide and oligosaccharide columns in Ag+ form.


Material Description pH StabilityCartridges for General Purpose/Pharmaceutical /10pk /10pk /3pk ea eaC18 (ODS, Octadecyl) 1.5 - 10 AJ0-4286 AJ0-4287 AJ0-7221 AJ0-7839 AJ0-8301C12 (Dodecyl) 1.5 - 10 AJ0-6073 AJ0-6074 AJ0-7275 AJ0-7842 AJ0-8304C8 (MOS, Octyl) 1.5 - 10 AJ0-4289 AJ0-4290 AJ0-7222 AJ0-7840 AJ0-8302C5 (Pentyl) 1.5 - 10 AJ0-4292 AJ0-4293 AJ0-7372 — —C1 (TMS) 2 - 9 AJ0-4298 AJ0-4299 AJ0-7373 — —Silica — — AJ0-4347 AJ0-4348 AJ0-7223 AJ0-7229 AJ0-8312HILIC HILIC 1.5 - 8 AJ0-8328 AJ0-8329 — — —NH

2 (Amino, Aminopropyl) 1.5 - 11 AJ0-4301 AJ0-4302 AJ0-7364 AJ0-8162 AJ0-8309CN (Cyano, Cyanopropyl) 2 - 7.5 AJ0-4304 AJ0-4305 AJ0-7313 AJ0-8220 AJ0-8311Phenyl (Phenylhexyl) 1.5 - 10 AJ0-4350 AJ0-4351 AJ0-7314 AJ0-7841 AJ0-8303PFP(2) Pentafluorophenylpropyl 1.5 - 8 AJ0-8326 AJ0-8327 AJ0-8376 AJ0-8377 AJ0-8378SCX (SA, Strong Cation Exchanger) 2.5 - 7.5 AJ0-4307 AJ0-4308 AJ0-7369 — —SAX (SB, Strong Anion Exchanger) 2.5 - 7.5 AJ0-4310 AJ0-4311 AJ0-7370 — —RP-1 (Reversed Phase - Polymer) 0 - 14 AJ0-5808 AJ0-5809 AJ0-7368 AJ0-8358 —Polar-RP (Ether-linked Phenyl) 1.5 - 7 AJ0-6075 AJ0-6076 AJ0-7276 AJ0-7845 AJ0-8307Fusion-RP (C18 Polar Embedded) 1.5 - 10 AJ0-7556 AJ0-7557 AJ0-7558 AJ0-7844 AJ0-8306AQ C18 (Polar Endcapped C18) 1.5 - 7.5 AJ0-7510 AJ0-7511 AJ0-7512 AJ0-7843 AJ0-8305Gemini-NX (C18 TWIN-NX™ Technology) 1 - 12 AJ0-8367 AJ0-8368 AJ0-8369 AJ0-8370 AJ0-8371Gemini C18 (C18 TWIN Technology) 1 - 12 AJ0-7596 AJ0-7597 AJ0-7598 AJ0-7846 AJ0-8308Gemini C6-Phenyl (C6-Phenyl TWIN Technology) 1 - 12 AJ0-7914 AJ0-7915 — — —Oligo-RP (C18 TWIN Technology) 1 - 12 AJ0-8134 AJ0-8135 AJ0-8136 AJ0-8210 AJ0-8310Oligo-WAX (WA, Weak Anion Exchanger) 1.5 - 11 — AJ0-8324 AJ0-8325 — AJ0-8420Cartridges for Protein and Polypeptide Reversed Phase /10pk /10pk /3pk ea eaFor use with silica columns for separation of proteins & peptides, such as Jupiter® (Phenomenex); Vydac® 218TP, 214TP (Alltech Associates, Inc.); SynChropak® 300 C18, C4 (Eprogen, Inc.); Nucleosil® 300 Å C18, C4; HyPErSIl® 300 Å, and other widepore or 300 Å brands.Widepore C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4320 AJ0-4321 AJ0-7224 AJ0-7230 AJ0-8313Widepore C5 (Pentyl) 1.5 - 10 AJ0-4326 AJ0-4327 AJ0-7371 — —Widepore C4 (Butyl) 1.5 - 10 AJ0-4329 AJ0-4330 AJ0-7225 AJ0-7231 AJ0-8314Cartridges for Silica GFC — /10pk /3pk — —(Aqueous SEC) For use with silica GFC columns, such as BioSep (Phenomenex); ZOrBAX® GF-Series; Bio-Sil® (Bio-rad).GFC-2000 — 2 - 7.5 — AJ0-4487 AJ0-7365 — —GFC-3000 — 2 - 7.5 — AJ0-4488 AJ0-7366 — —GFC-4000 — 2 - 7.5 — AJ0-4489 AJ0-7367 — —Cartridges for Chiral /10pk /10pk /3pk ea eaFor use with chiral columns, such as lux™ Cellulose-1,Cellulose-2 & Amylose-2 (Phenomenex); CHIrAlCEl® OD-H® & CHIrAlPAk® AD®-H (DAICEl Chemical Industries ltd.)Lux™ Amylose-2 Amylose tris (5-chloro-2-methyl-

phenylcarbamate)2 - 9 AJ0-8471 AJ0-8470 — AJ0-8473 AJ0-8474

Lux™ Cellulose-1 Cellulose tris (3, 5-dimethyl-phenylcarbamate)

2 - 9 AJ0-8402 AJ0-8403 AJ0-8404 AJ0-8405 AJ0-8406

Lux™ Cellulose-2 Cellulose tris (3-chloro-4-methylphenylcarbamate)

2 - 9 AJ0-8398 AJ0-8366 AJ0-8399 AJ0-8400 AJ0-8401

Cartridges for Carbohydrate/Organic Acid — /10pk — — —For organic acid and carbohydrate analysis, such as rezex™ (Phenomenex); Aminex® (Bio-rad); Interaction; Sugar-Pak™ (Waters).Carbo-H+ — 1 - 8 — AJ0-4490 — — —Carbo-Ag+* — Neutral — AJ0-4491 — — —Carbo-Pb+2 — Neutral — AJ0-4492 — — —Carbo-Ca+2 — Neutral — AJ0-4493 — — —Guard Cartridge Holders (one-time purchase only) /kit /holder /kit /kit

KJ0-4282 AJ0-7220 AJ0-8223 AJ0-8277

22

Column Protectionin-line Filters

remove particulates and eliminate split peaks

In-line filters placed between the injection valve and the column are recommended for all HPLC systems. The in-line filter can also catch pump particulates/debris by placing it between pump and injector.

KrudKatcher™ Ultra Stainless Steel (Analytical)

Fits virtually all UHPLC /HPLC columns 1.0 to 4.6 mm ID•

Pressure rated to 20,000 psi (1,375 bar)•

Extremely low dead volume minimizes sample peak dispersion•

The KrudKatcher Ultra filter body houses an integrated 0.5 µm 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system backpressure or dead volume (< 0.2 µL).

Stainless Steel (Analytical)

The 1.5 mm diameter filter is recommended for use with smaller diameter columns, microbore columns, and very short columns for fast HPLC procedures. The 3 mm diameter filter element is recommended for use with conventional 4.6 mm diameter columns.

KrudKatcher™ Classic PeeK or Biocompatible (Analytical)

Fit pressure rated to 5000 psi. The PEEK® filter body houses an integrated 0.5 µm HiFlo™ 316 stainless steel depth filter that efficiently removes microparticulates from the flow stream without contributing to system dead volume (2 µL). Fits columns 1.0 to 4.6 mm ID.

Stainless Steel (PreP)

The 21.2 mm diameter filter is recommended for use with 18-30 mm diameter columns and is pressure rated to 8,000 psi (551 bar).

Biocompatible (SemiPrep)

The biocompatible filter consists of a stainless steel body, two PEEK® end-fittings, and a separate replaceable PEEK® frit. This filter unit can be placed in the flow path before or after the column with little or no effect on peak shape. Pressure rated to 6,000 psi (413 bar) or columns 6 to 16 mm ID.


Ordering InformationPart No. Description Unit PriceAF0-0375 In-line Filter with 0.5 µm Porosity x 1.5 mm dia. filter eaAF0-0376 Replacement Filter Disks: 0.5 µm x 1.5 mm 5/pkAF0-0377 In-line Filter with 0.5 µm Porosity x 3 mm dia. filter eaAF0-0378 Replacement Filter Disks: 0.5 µm x 3 mm 5/pk

Ordering InformationPart No. Description Unit PriceAF0-5727 KrudKatcher Disposable Pre-Column Filter, 0.5 µm 10/pk

Ordering InformationPart No. Description Unit PriceAF0-7866 HPLC PREP Column In-line Filter, S.S., 2.0 µm

Porosity x 21.2 mm dia.ea

AF0-7867 Replacement In-Line Filter Disks, S.S., 2.0 µm Porosity x 21.2 mm dia.

5/pk

Ordering InformationPart No. Description Unit PriceAF0-8497 KrudKatcher Ultra Column In-Line Filter,

0.5 µm Porosity x 0.004 in. ID3/pk

Wrenches not provided. KrudKatcher Ultra requires 5/16 in ID.

Ordering InformationPart No. Description Unit PriceAF0-8420 HPLC SemiPrep Column In-Line Filter

2.0 µm Porosity x 10 mm dia. filter, Biocompatibleea

AF0-8428 Replacement In-Line Filter Disk, PEEK, 2.0 µm Porosity x 10 mm dia.

5/pk

23


Sample Preparation

Column Protection

Terms and Conditions Subject to Phenomenex standard Terms and Conditions which may be viewed at www.Phenomenex.com/TermsAndConditions.

Trademarks Strata is a registered trademark of Phenomenex, Inc. FilterSys, KrudKatcher, Phenex, SecurityGuard, Solvent Saver, and Strata-X are trademarks of Phenomenex, Inc. Delrin, Nylon66, and Teflon are trademarks of E.I. du Pont de Nemours and Co. PEEK is a registered trademark of ICI Americas. Cyrolite is a registered trademark of CY/RO Industries.

SecurityGuard is patented by Phenomenex. U.S. Patent No. 5,863,428 Strata-X is patented by Phenomenex. U.S. Patent No. 7,119,145

© 2010 Phenomenex, Inc. All rights reserved.

If you are not completely satisfied with any of the products featured in this brochure, return within 45 days for a FULL REFUND.


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All other countries: Corporate office USA (310) 212-0555 (310) 328-7768 [email protected]

www.phenomenex.comPhenomenex products are available worldwide. For the distributor in your country, contact Phenomenex USA, International Department at [email protected]

BR

6575

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8_I

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