Figure_S2
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Figure S2 - Effect of maltotriose on MalT-H131A self-association in the absence of ATP. (A) MalT-H131A (10 µM) was preincubated anfd fitered through a Superdex 200 column in the absence of effectors or in the presence of maltotriose alone, as described in Material and Methods. (B) As in (A) except that the concentration of MalT-H131A in the injected samples was varied. 0.9 1 1.1 1.2 1.3 1.4 1 10 - maltotriose + maltotriose MalT-H131A (µM) 1 1.5 2 Vo Ve (ml) Vt Malt-T-H131A (10 µM ) - maltotriose + maltotriose 0.02 AU A B
description
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Transcript of Figure_S2
Figure S2 - Effect of maltotriose on MalT-H131A self-association in the absence of ATP. (A) MalT-H131A (10 µM) was preincubated anfd fitered through a Superdex 200 column in the absence of effectors or in the presence of maltotriose alone, as described in Material and Methods. (B) As in (A) except that the concentration of MalT-H131A in the injected samples was varied.
0.9
1
1.1
1.2
1.3
1.4
1 10
- maltotriose
+ maltotriose
MalT-H131A (µM)
Elution volume (ml)
1 1.5 2
0.91
Vo
1.12
Ve (ml)
Vt
Malt-T-H131A (10 µM )
- maltotriose
+ maltotriose
1.37
1.02
0.02 AU
A 280
A B
