Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.
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Transcript of Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.
![Page 1: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/1.jpg)
Fig. S1
CytoplasmVc
PeriplasmVpp
MicroenvironmentVe
DppAi App Ae
ad
k
IM OM
*
ad
*
ad
*
D
![Page 2: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/2.jpg)
1
)1(v
1
1impv
A
Type IIaType Ia
Aik Ae
D
Vc
da da
*
Ve
*
B
k DVc
da da
*
Ve
*
Ai Ae
Dimp
Fig. S2
![Page 3: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/3.jpg)
102
103
104
105
106
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Per
cent
effe
ctor
act
ivat
ion
0
40
60
20
i
i
AK
AEE
max
80
Basal Induction
level
c
e
V
Vv
vEE
max
Fig. S3
102 106
Ve/Vc
103 104 105
![Page 4: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/4.jpg)
102
103
104
105
-100
0
100
200
300
400
500
600
E nM hr-1
0
Shutoff Induction
200
400
500
Eopt
OFF
RS
Q
Emax
P
Fig. S4
Ve/Vc
102 105104103
![Page 5: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/5.jpg)
0 100 200 300 400 500 600 700 800 900 10000
50
100
150
200
250
300
Eopt EmaxCost
Benefit fmax
Activation E
Cos
t or
Ben
efit
Fig. S5
![Page 6: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/6.jpg)
A B
v
Low Benefit
vopt= 0
100 101 103 105
Cel
l den
sity
nT c
ells
/ml
102 104
×106
2.2
1
0.8
1.4
1.8
105 106 107 108C
ell d
ensi
ty n
T c
ells
/ml
104
106
108
High Benefit
vopt = inf
v
Fig. S6
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Abe
P
Abi
Sensor
E
Ve
P +
*
Fig. S7
![Page 8: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/8.jpg)
C
OFF
ONv
da hr-110-1 100 101 102
100
101
102
103
10-1
Type I
Type II
Fig. S8
![Page 9: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/9.jpg)
Fig. S9
Aik
Aik
Aik
Ae
D
V VB
D
D
SP
N
+
sensorSP
N
+
sensor
SP
N
+
sensor
A
![Page 10: Fig. S1 Cytoplasm V c Periplasm V pp Microenvironment V e D pp AiAi A pp AeAe k IMOM * * * D.](https://reader036.fdocuments.in/reader036/viewer/2022082805/55142982550346e7488b5bee/html5/thumbnails/10.jpg)
0
1
2
3
4
5
6
7
8
Agr Comx CSF2 CAI-1 CSP LuxS* CSP* LuxS CSF1
0
1
2
3
4
5
6
7
8
Rhl Car Lux Las Cin Tra Cep Ain Sin HAI-1
Log10(v)
vcalculated
vobserved
Log10(v)
vcalculated
vobserved
Fig. S10A
B
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Log10(vcalculated)1 2 3 4 5 6 7
Log 1
0(v o
bser
ved)
1
2
3
4
5
6
7
sin
CSF1
cin
luxrhl
lastra
CSF2
luxS
car
ain
HAI1
agrcomX
CAI-1
CSP*
CSPluxS*
Fig. S11
cep