Fig. S1 Banno et al

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Fig. S1 Banno et 549 CA9 1429 V. longisporum 1773 V. dahliae ------------------------------------------------------------ cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg 609 CA9 1489 V. longisporum 1773 V. dahliae ------------------------------------------------------------ cctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgag cctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgag 669 CA9 1549 V. longisporum 1773 V. dahliae ------------------------------------------------------------ tcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgct tcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgct 729 CA9 1609 V. longisporum 1773 V. dahliae ------------------------------------------------------------ gcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccac gcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccac 789 CA9 1669 V. longisporum 1773 V. dahliae ------------------------------------------------------------ aactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcct aactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcct 849 CA9 1729 V. longisporum 1773 V. dahliae gttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataa gttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataa ------------------------------------------------------------ 909 CA9 1789 V. longisporum 1773 V. dahliae ------------------------------------------------------------ ggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccat ggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccat 969 CA9 1849 V. longisporum 1774 V. dahliae aggtagctttgcaatcgaatgcatggccccaaaacgggagcctncggcttaatttgactc aggtagctttgcaatcgaatgcatggccccaaaacgggagcctgcggcttaatttgactc ------------------------------------ggagcctgcggcttaatttgactc 1029 CA9 1909 V. longisporum 1798 V. dahliae aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc 1089 CA9 1969 V. longisporum 1858 V. dahliae ttgattttgtgggtggtggtgcatg ttgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgctta ttgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgctta 1113 CA9 2029 V. longisporum 1918 V. dahliae attgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgc attgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgc cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg 1113 CA9 2089 V. longisporum 1978 V. dahliae cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg Fig. S1 Nucleotide sequences of the group I intron in the 18S rDNA gene of Verticillium longisporum to design the detection primers. Grey arrowed underscores mark forward (VlspF2) and reverse (VdspR2) primers used for the first-round of quantitative nested real-time (QNRT) PCR. Black arrowed underscores mark forward (VlspF1) and reverse (VlspR4) primers used for the second-round of QNRT-PCR. GenBank accessions for the nucleotide sequences of the 18S rDNA region: V. longisporum CA9, AB585938; V. longisporum, AF153421; V. dahliae, AF104926.

description

Fig. S1 Banno et al. CA9. 549. cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg. V. longisporum. 1429. cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg. V. dahliae. 1773. ------------------------------------------------------------. CA9. 609. - PowerPoint PPT Presentation

Transcript of Fig. S1 Banno et al

Page 1: Fig. S1 Banno et al

Fig. S1 Banno et al

549CA91429V. longisporum1773V. dahliae ------------------------------------------------------------

cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctgcgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg

609CA91489V. longisporum1773V. dahliae ------------------------------------------------------------

cctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgagcctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgag

669CA91549V. longisporum1773V. dahliae ------------------------------------------------------------

tcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgcttcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgct

729CA91609V. longisporum1773V. dahliae ------------------------------------------------------------

gcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccacgcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccac

789CA91669V. longisporum1773V. dahliae ------------------------------------------------------------

aactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcctaactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcct

849CA91729V. longisporum1773V. dahliae

gttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataagttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataa------------------------------------------------------------

909CA91789V. longisporum1773V. dahliae ------------------------------------------------------------

ggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccatggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccat

969CA91849V. longisporum1774V. dahliae

aggtagctttgcaatcgaatgcatggccccaaaacgggagcctncggcttaatttgactcaggtagctttgcaatcgaatgcatggccccaaaacgggagcctgcggcttaatttgactc------------------------------------ggagcctgcggcttaatttgactc

1029CA91909V. longisporum1798V. dahliae

aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttcaacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc

aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc

1089CA91969V. longisporum1858V. dahliae

ttgattttgtgggtggtggtgcatgttgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgcttattgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgctta

1113CA92029V. longisporum1918V. dahliae

attgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgcattgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgc

cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg

1113CA92089V. longisporum1978V. dahliae

cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg

Fig. S1 Nucleotide sequences of the group I intron in the 18S rDNA gene of Verticillium longisporum to design the detection primers. Grey arrowed underscores mark forward (VlspF2) and reverse (VdspR2) primers used for the first-round of quantitative nested real-time (QNRT) PCR. Black arrowed underscores mark forward (VlspF1) and reverse (VlspR4) primers used for the second-round of QNRT-PCR. GenBank accessions for the nucleotide sequences of the 18S rDNA region: V. longisporum CA9, AB585938; V. longisporum, AF153421; V. dahliae, AF104926.

Page 2: Fig. S1 Banno et al

ttcttagcgaaccattaaaacttttaacaacggatctcttggctctagcatcgatgaaga

ttcttagcgaaccattaaaacttttaacaacggatctcttggctctagcatcgatgaaga

ttcttagcgaactattaaaacttttaacaacggatctcttggctctagcatcgatgaaga

ttcttagcgaactattaaaacttttaacaacggatctcttggctctagcatcgatgaaga

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132

118

CA9

V. longisporum

V. albo-atrum

V. dahliae

172

171

192

178

CA9

V. longisporum

V. albo-atrum

V. dahliae

acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg

acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg

acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg

acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg

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231

252

238

CA9

V. longisporum

V. albo-atrum

V. dahliae

aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac

aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgagacgtcgtttcaac

aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac

aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac

291

291

311

297

CA9

V. longisporum

V. albo-atrum

V. dahliae

cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg

cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg

cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg

cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg

52

51

72

58

CA9

V. longisporum

V. albo-atrum

V. dahliae

cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg

cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg

cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg

cgttctgcgagcccgccggtccatcagtctctctgtttataccaacgatacttctgagtg

cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca351

351

371

357

CA9

V. longisporum

V. albo-atrum

V. dahliae

cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca

cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca

cggacccgcgtggcccttccttgcgtagtagttacagctcgcatcggagtcccgcaggcg

Fig. S2 Banno et al

Fig. S2 Nucleotide differences in the 5.8S rDNA-ITS region among Verticillium longisporum, V. albo-atrum and V. dahliae to design the detection primers. Differences in the four sequences are indicated by black boxes. Black arrowed underscores mark forward (VaF1) and reverse (VaR1) primers used for V. longisporum 5.8S rDNA-ITS. Grey arrowed underscores mark forward (VdF1) and reverse (VdR1) primers used for V. dahliae 5.8S rDNA-ITS. GenBank accessions for nucleotide sequences of the 5.8S rDNA-ITS region: V. longisporum CA9, AB585937; V. longisporum, AY555951; V. albo-atrum, GU291258; V. dahliae, EU627007.