Fig. S1 Banno et al
description
Transcript of Fig. S1 Banno et al
Fig. S1 Banno et al
549CA91429V. longisporum1773V. dahliae ------------------------------------------------------------
cgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctgcgcgtcccagagccaaactgcctctgggtcgtggatctgcctctgaattcacggcatctg
609CA91489V. longisporum1773V. dahliae ------------------------------------------------------------
cctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgagcctccgaattcagggcaactgcctaacaggcggttggttctgattcaggaccagcctgag
669CA91549V. longisporum1773V. dahliae ------------------------------------------------------------
tcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgcttcacgagagatatggggaaggttcagagacttgacgggggtgggtgaattcactgctgct
729CA91609V. longisporum1773V. dahliae ------------------------------------------------------------
gcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccacgcaacaatataaatggggagagatcctcttcttcttcttccaacaaccaaaccaaaccac
789CA91669V. longisporum1773V. dahliae ------------------------------------------------------------
aactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcctaactgaacctcaaacaagacccacaagctcttcaaaatgtcccattcttctccctctcct
849CA91729V. longisporum1773V. dahliae
gttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataagttctcgctaacgggagcgagtatgtcgtgagggatggaggttcgtctggtcaggcataa------------------------------------------------------------
909CA91789V. longisporum1773V. dahliae ------------------------------------------------------------
ggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccatggaacgagaatgcagtggcgtggtttgcttaagataaagtccgggcttatgggaaaccat
969CA91849V. longisporum1774V. dahliae
aggtagctttgcaatcgaatgcatggccccaaaacgggagcctncggcttaatttgactcaggtagctttgcaatcgaatgcatggccccaaaacgggagcctgcggcttaatttgactc------------------------------------ggagcctgcggcttaatttgactc
1029CA91909V. longisporum1798V. dahliae
aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttcaacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc
aacacggggaaactcaccaggtccagacacaacgaggattgacagattgagagctctttc
1089CA91969V. longisporum1858V. dahliae
ttgattttgtgggtggtggtgcatgttgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgcttattgattttgtgggtggtggtgcatggccgttcttagttggtggagtgatttgtctgctta
1113CA92029V. longisporum1918V. dahliae
attgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgcattgcgataacgaacgagaccttaacctgctaaatagtccgtattaccccggtagtacgc
cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg
1113CA92089V. longisporum1978V. dahliae
cgacttcttagagggactatcggctcaagccgatggaagtttgaggcaataacaggtctg
Fig. S1 Nucleotide sequences of the group I intron in the 18S rDNA gene of Verticillium longisporum to design the detection primers. Grey arrowed underscores mark forward (VlspF2) and reverse (VdspR2) primers used for the first-round of quantitative nested real-time (QNRT) PCR. Black arrowed underscores mark forward (VlspF1) and reverse (VlspR4) primers used for the second-round of QNRT-PCR. GenBank accessions for the nucleotide sequences of the 18S rDNA region: V. longisporum CA9, AB585938; V. longisporum, AF153421; V. dahliae, AF104926.
ttcttagcgaaccattaaaacttttaacaacggatctcttggctctagcatcgatgaaga
ttcttagcgaaccattaaaacttttaacaacggatctcttggctctagcatcgatgaaga
ttcttagcgaactattaaaacttttaacaacggatctcttggctctagcatcgatgaaga
ttcttagcgaactattaaaacttttaacaacggatctcttggctctagcatcgatgaaga
112
111
132
118
CA9
V. longisporum
V. albo-atrum
V. dahliae
172
171
192
178
CA9
V. longisporum
V. albo-atrum
V. dahliae
acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg
acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg
acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg
acgcagcgaaacgcgatatgtagtgtgaattgcagaattcagtgaatcatcgaatctttg
232
231
252
238
CA9
V. longisporum
V. albo-atrum
V. dahliae
aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac
aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgagacgtcgtttcaac
aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac
aacgcacatggcgccttccagtatcctgggaggcatgcctgtccgag-cgtcgtttcaac
291
291
311
297
CA9
V. longisporum
V. albo-atrum
V. dahliae
cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg
cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg
cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg
cctcgagccccagtggcccggtgttggggatctacgtctgtaggcccttaaaagcagtgg
52
51
72
58
CA9
V. longisporum
V. albo-atrum
V. dahliae
cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg
cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg
cgttctgcgagcccgccggtacatcagtctctttatttataccaacgatacttctgagtg
cgttctgcgagcccgccggtccatcagtctctctgtttataccaacgatacttctgagtg
cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca351
351
371
357
CA9
V. longisporum
V. albo-atrum
V. dahliae
cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca
cggacccgcgtggcccttccttgcgtagtaattacagctcgcatcggagtcccgcaggca
cggacccgcgtggcccttccttgcgtagtagttacagctcgcatcggagtcccgcaggcg
Fig. S2 Banno et al
Fig. S2 Nucleotide differences in the 5.8S rDNA-ITS region among Verticillium longisporum, V. albo-atrum and V. dahliae to design the detection primers. Differences in the four sequences are indicated by black boxes. Black arrowed underscores mark forward (VaF1) and reverse (VaR1) primers used for V. longisporum 5.8S rDNA-ITS. Grey arrowed underscores mark forward (VdF1) and reverse (VdR1) primers used for V. dahliae 5.8S rDNA-ITS. GenBank accessions for nucleotide sequences of the 5.8S rDNA-ITS region: V. longisporum CA9, AB585937; V. longisporum, AY555951; V. albo-atrum, GU291258; V. dahliae, EU627007.