February 1, 2011 Staple and turn in lab. PLEASE make sure pages are in order and your name is on it....
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Transcript of February 1, 2011 Staple and turn in lab. PLEASE make sure pages are in order and your name is on it....
February 1, 2011• Staple and turn in lab. PLEASE make sure pages are in
order and your name is on it.• Return graded work• Review/Questions• Test-Organic Chemistry• Lecture-Cell Structure• Lab-Cell Structure• Homework:
– Lab Review Questions– Cell Structure Review– Study for Test
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Basic Structure of a Cell
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Review Facts Review Facts About Living About Living
ThingsThings
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What Are the Main What Are the Main Characteristics of Characteristics of
organisms?organisms?1. Made of CELLS2. Require ENERGY (food)3. REPRODUCE (species)4. Maintain HOMEOSTASIS5. ORGANIZED6. RESPOND to environment7. GROW and DEVELOP8. EXCHANGE materials with
surroundings (water, wastes, gases)
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LEVELS OF ORGANIZATIONLEVELS OF ORGANIZATION
Nonliving Levels:1.ATOM (element)2.MOLECULE (compounds like
carbohydrates & proteins)3.ORGANELLES (nucleus, ER,
Golgi …)
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LEVELS OF ORGANIZATIONLEVELS OF ORGANIZATION
Living Levels:4.CELL (makes up ALL organisms) –
LIFE begins5.TISSUE (cells working together6.ORGAN (heart, brain, stomach …)7.ORGAN SYSTEMS (respiratory,
circulatory …)8.ORGANISM
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LEVELS OF ORGANIZATIONLEVELS OF ORGANIZATIONLiving Levels continued:9.POPULATION (one species in an area)10.COMMUNITY (several populations in
an area11.ECOSYSTEM (forest, prairie …)12.BIOME (Tundra, Tropical Rain
forest…)13.BIOSPHERE (all living and nonliving
things on Earth)
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History of Cells & the History of Cells & the Cell TheoryCell Theory
Cell Specialization
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First to View CellsFirst to View Cells•Robert Hooke -
microscope to examine a thin slice of cork
• looked like small boxes
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First to View CellsFirst to View Cells•Hooke -
responsible for naming cells
•“CELLS” – because small rooms that monks lived in called Cells
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Anton van Leeuwenhoek
• Leeuwenhoek (Dutch microscope maker), - first to view organisms
• used a simple, handheld microscope to view pond water & scrapings from his teeth
MICROSCOPESMICROSCOPES
12Copyright Cmassengale
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Microscopy and Microscopy and MeasurementMeasurement
• Microscopes – produce an produce an enlarged image of an objectenlarged image of an object– Used to study organisms, cells, and cell parts– Increase in apparent size is called
magnificationmagnification– The ability to show details clearly is called
resolutionresolution– Microscopes vary in both magnification and
resolution
Copyright Cmassengale
14Copyright Cmassengale
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Compound Light Compound Light MicroscopesMicroscopes
• SpecimenSpecimen mounted on a glass slideglass slide
• Must be thinly sliced or very small
• Pair of lenseslenses– OcularOcular lens (eye piece)– ObjectiveObjective lens (nose
piece)• Can be used to study
LIVE specimensLIVE specimens
Copyright Cmassengale
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• MagnificationMagnification determined by multiplying power of both lenses
• Eyepiece 10X times Objective power (20X, 40X…)
• HighestHighest Maximum magnification is around 1000X1000X
Copyright Cmassengale
17
Electron MicroscopeElectron Microscope• Transmission EM (TEM)Transmission EM (TEM)
– Uses a beam of electrons beam of electrons to produce an enlarged image of very thinly sliced specimen on screen or photographic plate
– Image focused by magnetic magnetic lenseslenses
– 200,000X magnification– Cannot be used to view living
specimens
Copyright Cmassengale
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• Scanning EM (SEM)Scanning EM (SEM)– 3D3D image– Specimens not sliced for not sliced for
viewingviewing– Surface sprayed with fine Surface sprayed with fine
metal coatingmetal coating– Also uses electron beam and
fluorescent screen or photographic plates
– 100,000X magnification100,000X magnification– Cannot be used to view living Cannot be used to view living
specimensspecimens
Copyright Cmassengale
19
Beginning of the Cell Beginning of the Cell TheoryTheory
•Matthias Schleiden – botanist, concluded all plants were made of cells
•a cofounder of the cell theory
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Beginning of the Cell Beginning of the Cell TheoryTheory
•Theodore Schwann – zoologist, concluded all animals were made of cells
•also cofounded the cell theory
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Beginning of the Cell Beginning of the Cell TheoryTheory
• Rudolph Virchow – doctor, observed cells dividing under the microscope
• reasoned that all cells come from other pre-existing cells by cell division
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CELL THEORY
•All living things are made of cells
•Cells are the basic unit of structure and function in an organism (basic unit of life)
•Cells come from the reproduction of existing cells (cell division)
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Cell Size and TypesCell Size and Types
• Cells can only be observed under microscope
• Three Basic types:
Animal Cell Plant CellBacterial
Cell
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Number of CellsNumber of CellsOrganisms may be:• Unicellular – composed of one cell
• Multicellular- composed of many cells that may organize into tissues, etc.
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CELL SIZECELL SIZE
Typical cells range from 5 – 50 micrometers (microns) in diameter
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Which Cell Type is Which Cell Type is Larger?Larger?
_________ > _____________ > ___________Plant cell Animal cell bacteria
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How Big is a Micron ( µ ) ?How Big is a Micron ( µ ) ?
1 cm = 10,000 microns 1” = 25,000 microns
1 mm = 1000 microns
28
Multicellular OrganismsMulticellular Organisms• Cells in multicellular organisms
often specialize (take on different shapes & functions)
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Cell SpecializationCell Specialization
•Specialization - turning different genes on and off
•DIFFERENTIATION
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Specialized Animal CellsMuscle cells Red blood cells
Cheek cells
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Specialized Plant cells
Xylem cellsPollen
Guard Cells
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Organization Organization Levels of LifeLevels of Life
Atoms to OrganismsAtoms to Organisms
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ATOMS MOLECULES ORGANELLES
Nonliving LevelsNonliving Levels
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CELLS – life starts here
TISSUES – Similar cells working together
Living LevelsLiving Levels
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ORGANSORGAN SYSTEMS ORGANISM
Different tissuesworking together
Different organsworking together
More Living LevelsMore Living Levels
36
Simple or Complex Simple or Complex CellsCells
37
Prokaryotes – The first Prokaryotes – The first CellsCells
• Cells that lack a nucleus or membrane-bound organelles
• Includes bacteria• Simplest type of cell• Single, circular chromosome
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ProkaryotesProkaryotes
• Nucleoid region (center) contains the DNA
• Surrounded by cell membrane & cell wall (peptidoglycan)
• Contain ribosomes (no membrane) in their cytoplasm to make proteins
39
Eukaryotes
• Cells that HAVE a nucleus and membrane-bound organelles
• Includes protists, fungi, plants, and animals
• More complex type of cells
40
Eukaryotic Cell
Contain 3 basic cell structures:
•Nucleus•Cell Membrane•Cytoplasm with
organelles
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Two Main Types of Eukaryotic Cells
Plant Cell
Animal Cell
42
OrganellesOrganelles
43
OrganellesOrganelles
•Very small (Microscopic)•Perform various functions for
a cell•Found in the cytoplasm•May or may not be
membrane-bound
44
Animal Cell OrganellesAnimal Cell Organelles
NucleolusNucleus
Nuclear envelope
Ribosome (attached)Ribosome (free)
Cell Membrane
Rough endoplasmic reticulum
Golgi apparatus
Mitochondrion
Smooth endoplasmicreticulum
Centrioles
45
Plant Cell OrganellesPlant Cell Organelles
46
Cell or Plasma MembraneCell or Plasma Membrane
Outsideof cell
Insideof cell(cytoplasm)
Cellmembrane
Proteins
Proteinchannel Lipid bilayer
Carbohydratechains
• Composed of double layer of phospholipids and proteins
• Surrounds outside of ALL cells• Controls what enters or leaves the cell• Living layer
47
•Lies immediately against the cell wall in plant cells
•Pushes out against the cell wall to maintain cell shape
Cell Membrane in PlantsCell Membrane in PlantsCell membrane
48
•Nonliving layer•Found in plants, fungi, & bacteria
•Made of cellulose in plants
•Made of peptidoglycan in bacteria
•Made of chitin in Fungi
Cell wallCell WallCell Wall
49
Cell WallCell Wall• Supports and
protects cell• Found outside of
the cell membrane
50
•Jelly-like substance enclosed by cell membrane
•Provides a medium for chemical reactions to take place
Cytoplasm of a Cytoplasm of a Cell Cell
cytoplasm
51
•Contains organelles to carry out specific jobs
•Found in ALL cells
More on CytoplasmMore on Cytoplasmcytoplasm
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•Controls the normal activities of the cell
•Contains the DNA in chromosomes
•Bounded by a nuclear envelope (membrane) with pores
•Usually the largest organelle
The Control Organelle - The Control Organelle - NucleusNucleus
53
Nuclear EnvelopeNuclear Envelope
• Double membrane surrounding nucleus
• Also called nuclear membrane
• Contains nuclear pores for materials to enter & leave nucleus
• Connected to the rough ER Nuclear
pores
54
Nucleolus
• Inside nucleusInside nucleus• Cell may have Cell may have 1 1
to 3to 3 nucleoli nucleoli• DisappearsDisappears
when cell when cell dividesdivides
• Makes Makes ribosomesribosomes that that make proteinsmake proteins
55
CytoskeletonCytoskeleton• Helps cell maintain
cell shape• Also help move
organelles around• Made of proteins• Microfilaments are
threadlike & made of ACTIN
• Microtubules are tubelike & made of TUBULIN
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CytoskeletonCytoskeleton
MICROTUBULES
MICROFILAMENTS
57
CentriolesCentrioles• Found only in animal
cells• Paired structures
near nucleus• Made of bundle of
microtubules• Appear during cell
division forming mitotic spindle
• Help to pull chromosome pairs apart to opposite ends of the cell
58
Centrioles & the Mitotic Centrioles & the Mitotic SpindleSpindle
Made of MICROTUBULES (Tubulin)
59
MitochondrionMitochondrion(plural = mitochondria)(plural = mitochondria)
• “Powerhouse” of the cell
• Generate cellular energy (ATP)
• More active cells like muscle cells have MORE mitochondria
• Both plants & animal cells have mitochondria
• Site of CELLULAR RESPIRATION (burning glucose)
60
MITOCHONDRIASurrounded by a
DOUBLE membrane
Folded inner membrane called CRISTAE (increases surface areafor more chemical Reactions)
Has its own DNA
Interior called MATRIX
61
Interesting Fact ---Interesting Fact ---
• Mitochondria Come from cytoplasm in the EGG cell during fertilization
Therefore …• You inherit your
mitochondria from your mother!
62
Rod shape
Cell PowerhouseCell Powerhouse
Mitochondrion( mitochondria )
63
Endoplasmic Reticulum - EREndoplasmic Reticulum - ER
Two kinds of ER ---ROUGH & SMOOTH
• Network of hollow membrane tubules• Connects to nuclear envelope & cell
membrane• Functions in Synthesis of cell products
& Transport
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Rough Endoplasmic Reticulum Rough Endoplasmic Reticulum (Rough ER)(Rough ER)
• Has ribosomes on its surface
• Makes membrane proteins and proteins for EXPORT out of cell
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Smooth Endoplasmic Smooth Endoplasmic ReticulumReticulum
• Smooth ER lacks ribosomes on its surface
• Is attached to the ends of rough ER
• Makes cell products that are USED INSIDE the cell
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Functions of the Smooth Functions of the Smooth ERER• Makes membrane
lipids (steroids)• Regulates calcium
(muscle cells)• Destroys toxic
substances (Liver)
67
Endomembrane System
Includes nuclear membrane connected to ER connected to cell membrane (transport)
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RibosomesRibosomes• Made of PROTEINS and rRNA• “Protein factories” for cell• Join amino acids to make proteins• Process called protein synthesis
69
RibosomesRibosomes
Can be attached to Rough ER
OR
Be free (unattached
) in the cytoplasm
70
Golgi BodiesGolgi Bodies• Stacks of Stacks of flattened flattened
sacssacs• Have a shipping side Have a shipping side
((trans facetrans face) and ) and receiving side (receiving side (cis cis faceface))
• Receive Receive proteinsproteins made by ERmade by ER
• Transport vesiclesTransport vesicles with modified with modified proteins pinch off the proteins pinch off the endsends
Transport vesicle
CIS
TRANS
71
GolgiGolgi
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LysosomesLysosomes• Contain Contain digestive digestive
enzymesenzymes• Break down Break down food, food,
bacteria,bacteria, and and worn out worn out cell partscell parts for cells for cells
• Programmed forProgrammed for cell cell death (apoptosis)death (apoptosis)
• Lyse (break open) & Lyse (break open) & release enzymesrelease enzymes to to break down & recycle break down & recycle cell parts)cell parts)
73
Cilia & FlagellaCilia & Flagella
• Made of protein tubes called microtubules
• Function in moving cells, in moving fluids, or in moving small particles across the cell surface
74
Cilia & FlagellaCilia & Flagella
• Cilia are shorter and more numerous on cells
• Flagella are longer and fewer (usually 1-3) on cells
75
Cell Movement with Cilia Cell Movement with Cilia & Flagella& Flagella
76
Cilia Moving Away Dust Particles Cilia Moving Away Dust Particles from the Lungsfrom the Lungs
Respiratory SystemRespiratory System
77
VacuolesVacuoles• Fluid filled sacks for storage• Small or absent in animal cells• Plant cells have a large Central Vacuole• No vacuoles in bacterial cells
78
VacuolesVacuoles
• In plants, they store Cell Sap
• Includes storage of sugars, proteins, minerals, lipids, wastes, salts, water, and enzymes
79
Contractile Contractile VacuoleVacuole
• Found in unicellular protists like paramecia
• Regulate water intake by pumping out excess (homeostasis)
• Keeps the cell from lysing (bursting)
Contractile vacuole animation
80
ChloroplastsChloroplasts• Found only in producers (organisms
containing chlorophyll)• Use energy from sunlight to make
own food (glucose)• Energy from sun stored in the
Chemical Bonds of Sugars
81
ChloroplastsChloroplasts• Surrounded by DOUBLE
membrane• Outer membrane smooth• Inner membrane
modified into sacs called Thylakoids
• Thylakoids in stacks called Grana & are interconnected
• Stroma – gel like material surrounding thylakoids
82
ChloroplastsChloroplasts
• Contains its own DNA
• Contains enzymes & pigments for Photosynthesis
• Never in animal or bacterial cells
• Photosynthesis – food making process
QUICK REVIEW!
83
central vacuole
storage: food,
water or waste
mitochondriamake ATP in cellular respiration
chloroplastmake ATP & sugars in photosynthesis
cell wallsupport
cell membranecell boundarycontrols movementof materials in & out
recognizes signals
Golgi apparatus
finish & ship proteins
nucleuscontrol cellprotects DNA
endoplasmic reticulum
processes proteinsmakes membranes
lysosomedigestion & clean up
ribosomesmake proteins
cytoplasmjelly-like material
around organelles
nucleolusmake ribosomes
QUESTIONS?
86
87
Cheek Cells
Onion Cells