Accurate Mass TOF LC/MS Systems combined with powerful MassHunter data processing provides answers to your recombinant protein questions.

FAST RECOMBINANT PROTEIN CHARACTERIZATION WITH ACCURATE MASS LC/MS

Robust, Reliable, and Easy to Use

Use TOF LC/MS to determine

• Intact Protein Mass

• Peptide Mapping

• Post-translational Modifications

• Glycan Analysis

For more information visit: www.agilent.com/chem/biopharma

145000 146000

145924.80

147370.11

148816.02

147000 148000 149000

Rapid Glycoform Analysis by TOF LC/MSAccurate Intact Protein AnalysisAgilent Accurate-Mass TOF and Q-TOF systems simplify your routine protein and mAb analyses. Precise protein/peptide masses are displayed quickly with Agilent’s BioConfirm Software.

Glycan Profiling Made EasyFast Glycan Analysis in just ten minutes with Agilent Q-TOF and the Agilent glycan MS database.

Glycan Profiling

GO-G

N-F N

G1N

GON

GO-F

NM

an6N Man

5N

G1-G

N-F N

G1-G

N NG1

N G1N

G2N

G2N

G2N

Agilent Products are for Research Use Only.Not for use in diagnostic procedures.

Information, descriptions and specifications in thispublication are subject to change without notice.

© Agilent Technologies, Inc. 2013Published in the USA, April 3, 2013

5991-2080EN

See how Agilent Accurate Mass LC/MS technology is ideally suited for the routing

analysis of biotherapeutics: www.agilent.com/chem/biopharma

Find the Right System for Your LabLC/TOF or LC/Q-TOF bring the power of accurate mass to your essential protein characterization tasks.

Ensure ConsistencyComparison of Two Batches of mAbs reveals that batch 2 has a higher percentage and greater variety of sialylated glycan forms.

DescriptionAccurate Mass TOF

Accurate Mass Q-TOF

Intact Masses • •Glycosylation State • •Glycoforms • •Oxidation Changes • •Amino Acids Substitutions • •Peptide Mapping •DeNovo (MS/MS) Peptide Sequencing •DeNovo (MS/MS) Glycan Sequencing •

0%

10%

20%

30%

40%

50%

60%

Relat

ive P

erce

nt

Antibody Batch Comparability

G1 G0 G2

G0-fu

c

G1+s

ilalic

acid

NGN

A

G2+s

ilalic

acid

NGN

A

G1-fu

c

Man

5

G1-fu

c-Gl

cNac

+sia

lic a

cid N

GNA

G1-fu

c +sia

lic a

cid N

GNA

G2-fu

c

Man

8

Batch 1Batch 2

Walk-Up MS-TOF Analysis

Simplified set-up with pre-defined methods for routine characterization.

• Load samples, start method, walk-away

• No optimization needed

• Easy Tools for System Set-Up

• Results emailed to your office