Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering
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Transcript of Fabrication of an electrospun nanofibrous scaffold for use in the field of tissue engineering
Fabrication of an electrospun nanofibrous scaffold for use in the
field of tissue engineering
By: Shannon Daily & Tyler Crawford
Purpose
To create a polycaprolactone mesh which enables cell activity and seeks to eventually provide an application in the field of tissue engineering toward biomimetic skin graft.
Progress since last meeting
Spun 4 more meshes
Visited Janelia Farm and used SEM
Changed procedure slightly
Created Timeline
Electrospun Meshes
All 15 wt. % solution using acetic acid as solvent
15 kV 15cm Used syringe pump (flow rate .02 ml/s)
for first three meshes› Now using pipette to reduce beading
SEM ImagesMesh 1, Image aMagnification: 148.685 µm x 111.514 µm
SEM ImagesMesh 1, Image bMagnification: 11.643 µm x 8.732 µm
SEM ImagesMesh 2, Image aMagnification: 173.875 µm x 130.406 µm
SEM ImagesMesh 2, Image bMagnification: 24.607 µm x 18.455 µm
To be worked on:
View SEM images of new PCL meshes› Visiting Janelia Farm on March 9
Begin varying voltage of pure PCL Create chitosan/PCL solution Begin cell work Updates
› ED› wikipage
Bibliography
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