F æ 4 æ äScience and Engineering Biomolecularê ^ Á ¹ | ¡ w R H % E & e F Q [¯ H b d + ¯ D Q...
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ΞȜρȜιͼΡၷ؊ဥࠒȂၑ ય༫گഎၑทȂࡢșఘঊ শۼഎȆۼߗഎܥෝ൲ఠຈါȃ൚ ݪࡄഥঊڠࢥȆΗϋΩ·ৗڠࢥ၌ဥ ňŇő ൝ ΗϋΩ·ৗσΏέͿρȜΔ൝ڠاอΗϋΩ ·ৗϋΐΣͺςϋΈȂρͼήͼιȜΐϋΈခဥ خণاίυȜήٳอଲٮ୶ߐȃ ഥঊࠑশഎอ۷ख़Ȃഥঊอ ΗϋΩ·ৗहȂΗϋΩ·ৗڐ८Ȃळ༾ඤࢹ௮൲ ఠ۷ख़ȂΗϋΩ·ৗۼဥ łŕőȂńŢ ijĬ ၑڰৗশۼߗ൲ఠȂအșၑય خণاळ༾τασࡢఘτασخෝ ȃȂșٳอخণاίυȜήঀ ૧ࡐڠޢٳอॲڠႲࠈ ȃخণاဏȂΦιȜ ΠσΑΉȜσΏΑΞθഎ۷തયၑȂ هݪࡄఴ ड߃৽ါა 1. Hossain M.N et al. Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water. Sensors, 20(11), 3164, 2020 2. Inagaki S, et al. Imaging local brain activity of multiple freely moving mice sharing the same environment. Scientific Reports, 9, 7460, 2019 3. Suzuki K, et al. Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca 2+ microscopy. BMC Research Notes, 11(1),313,2018 4. Arai Y, et al. A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging. ACS Chemical Biology, 13 (8), 1938-1943, 2018 5. Riani YD, Matsuda T, Takemoto K, Nagai T. Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and cell ablation. BMC Biology 16, 1, 2018. 6. Kushida Y, Arai Y, SHimono K, Nagai T. Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform. ACS Sensors, 3, 87-92, 2018 7. Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. Atransient rise in free Mg 2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation. Current Biology, 28, 444-451, 2018 8. Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T., Acid-tolerant monomeric GFP from Olindias formosa., Cell Chemical Biology, 25, 330-338, 2018 9. Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda T, Nagai T. Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Scientific Reports, 7, 42398, 2017 10. Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T.: Five color variants of bright luminescent protein for real-time multicolor bioimaging. Nature Communications, 7, 13718, 2016 11. Tiwari DK, et al. Fast positively-photoswitchable fluorescent protein for ultra-low laser power RESOLFT nanoscopy. Nature Methods, 12, 515-518, 2015. １）蛍光および発光蛋白質を用いたセンサー開発 ２）発光センサーを利用した化合物スクリーニングシステム の開発 ３）オンサイト生理活性分子計測技術の開発 Biomolecular Science and Engineering 台諦木匠揮協木肋 教 授 永井 健治 Professor Takeharu NAGAI 06-6879-8480 [email protected] 准教授 松田 知己 Associate Professor Tomoki MATSUDA 06-6879-8481 [email protected] 助 教 服部 満 Assistant Professor Mitsuru HATTORI 06-6879-8481 [email protected] 助 教 長部 謙二 Assistant Professor Kenji OSABE 06-6879-8481 [email protected] 特任教授 宮崎 純一 Specially Appointed Prof. Jun-ichi MIYAZAKI 06-6105-5934 [email protected] 特任准教授 和沢 鉄一 Specially Appointed Assoc. Prof. Tetsuichi WAZAWA 06-6879-8481 [email protected] 特任助教 圓谷 徹之 Specially Appointed Assis. Prof. Tetsuyuki ENTANI 06-6879-8481 [email protected] 教 授 永井 健治 図２ 化学発光プローブを用いたオンサイト生理活性分子計測の概念図 図１ 化学発光プローブによるマウス個体を用いた分子活性評価の概念図 ｈｙ糖細っ狂Ｐｂｑ喋歪さ装燈ィガびよｙ肝侈陪休こ卒 遭ｂｑＩぜ｠凩Ｍぁ‒テそゥ企七蒙っ妃蒙昨諦トサデｒ よまテそェハみｄぜゆよアゼｙ休保さ‒佝しｕ台偬狂胎 木匠っ遇宥ぃ則嬬ぃ燃もよイｒべハやびイ婚坪嬉赴ｕ潔 澄ｙ休保ｕｔっ乎ＷぜＸｓＱｒＲぜ｠筑燭ｚ酔倔ｙ喋歪 ｙ蒐せ様っ諭ＭぜこｙｒＧせ‒槽歪ｙ休保ｖ天ＲＴ磁采 ｄぜｓ渓鼎Ｚそぜ｠撒燭ｙ潔澄ｚ偽しＱ休保ｂｑＲｊ嬉 昌企コドそゲｙ保伺ゆみウデっよズそイケへハｒ椿Ｍｑ 台偬狂胎木匠ｙ負肌班っ悩冉婚初ｄぜＸｓｖ契ｐＩｑＩ ぜ｠ＸｙすＫｕ潔澄ｙ遭陪ｖすｎｑ‒酔倔鱒穫ｒ爾だそ ｑＩｊ栽取Ｑ訟点ｒ榛昏ｖ爾ＭぜすＫｖｕせ‒Ｑつっ勲 け嵩飛ｕ嬢鱒ｙ脹渓保細ｚこｓすせ‒鱒穫宵誰Ｑ袋ｎｑ ＩｕＩ硲禅質ｖＯＶぜゴデよむぱ袖整ｙ士禅ｕｔｖこ磁 采ｄぜｓ渓鼎Ｚそぜ｠ 37
Transcript of F æ 4 æ äScience and Engineering Biomolecularê ^ Á ¹ | ¡ w R H % E & e F Q [¯ H b d + ¯ D Q...
1. Hossain M.N et al. Bioluminescent Ratiometric Indicator for Analysis of Water Hardness in Household Water. Sensors, 20(11), 3164, 2020 2. Inagaki S, et al. Imaging local brain activity of multiple freely moving mice sharing the same environment. Scientific Reports, 9, 7460, 2019 3. Suzuki K, et al. Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence
Ca2+ microscopy. BMC Research Notes, 11(1),313,2018 4. Arai Y, et al. A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging. ACS Chemical Biology, 13
(8), 1938-1943, 2018 5. Riani YD, Matsuda T, Takemoto K, Nagai T. Green monomeric photosensitizing fluorescent protein for photo-inducible protein inactivation and
cell ablation. BMC Biology 16, 1, 2018. 6. Kushida Y, Arai Y, SHimono K, Nagai T. Biomimetic chemical sensing by fluorescence signals using a virus-like particle-based platform. ACS
Sensors, 3, 87-92, 2018 7. Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. Atransient rise
in free Mg2+ ions released from ATP-Mg by its hydrolysis contributes to mitotic chromosome condensation. Current Biology, 28, 444-451, 2018 8. Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T., Acid-tolerant monomeric GFP from Olindias formosa., Cell Chemical Biology,
25, 330-338, 2018 9. Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels M, Okamura Y, Matsuda T, Nagai T. Genetically encoded
bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Scientific Reports, 7, 42398, 201710. Suzuki K, Kimura T, Shinoda H, Bai G, Daniels M, Arai Y, Nakano M, and Nagai T.: Five color variants of bright luminescent protein for real-time
multicolor bioimaging. Nature Communications, 7, 13718, 201611. Tiwari DK, et al. Fast positively-photoswitchable fluorescent protein for ultra-low laser power RESOLFT nanoscopy. Nature Methods, 12, 515-518, 2015.
１）蛍光および発光蛋白質を用いたセンサー開発２）発光センサーを利用した化合物スクリーニングシステムの開発
３）オンサイト生理活性分子計測技術の開発
BiomolecularScience and Engineering
教 授 永井 健治 Professor Takeharu NAGAI 06-6879-8480 [email protected]准教授 松田 知己 Associate Professor Tomoki MATSUDA 06-6879-8481 [email protected]助 教 服部 満 Assistant Professor Mitsuru HATTORI 06-6879-8481 [email protected]助 教 長部 謙二 Assistant Professor Kenji OSABE 06-6879-8481 [email protected]特任教授 宮崎 純一 Specially Appointed Prof. Jun-ichi MIYAZAKI 06-6105-5934 [email protected]特任准教授 和沢 鉄一 Specially Appointed Assoc. Prof. Tetsuichi WAZAWA 06-6879-8481 [email protected]特任助教 圓谷 徹之 Specially Appointed Assis. Prof. Tetsuyuki ENTANI 06-6879-8481 [email protected]
教 授 永井 健治
図２ 化学発光プローブを用いたオンサイト生理活性分子計測の概念図
図１ 化学発光プローブによるマウス個体を用いた分子活性評価の概念図
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